Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6565 and GFF6566 are separated by 188 nucleotides GFF6566 and GFF6567 overlap by 4 nucleotides
GFF6565 - hypothetical protein, at 3,498 to 4,742
GFF6565
GFF6566 - hypothetical protein, at 4,931 to 7,594
GFF6566
GFF6567 - hypothetical protein, at 7,591 to 9,264
GFF6567
Position (kb)
4
5
6
7
8 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4.150 kb on - strand, within GFF6565 at 4.150 kb on - strand, within GFF6565 at 4.166 kb on - strand, within GFF6565 at 4.245 kb on + strand, within GFF6565 at 4.246 kb on - strand, within GFF6565 at 4.246 kb on - strand, within GFF6565 at 4.246 kb on - strand, within GFF6565 at 4.246 kb on - strand, within GFF6565 at 4.260 kb on - strand, within GFF6565 at 4.260 kb on - strand, within GFF6565 at 4.260 kb on - strand, within GFF6565 at 4.426 kb on + strand, within GFF6565 at 4.427 kb on - strand, within GFF6565 at 4.669 kb on - strand at 4.799 kb on + strand at 4.820 kb on + strand at 4.870 kb on - strand at 4.923 kb on + strand at 4.924 kb on - strand at 4.981 kb on - strand at 5.033 kb on + strand at 5.034 kb on - strand at 5.034 kb on - strand at 5.196 kb on + strand at 5.197 kb on - strand at 5.197 kb on - strand at 5.241 kb on + strand, within GFF6566 at 5.244 kb on + strand, within GFF6566 at 5.267 kb on + strand, within GFF6566 at 5.267 kb on + strand, within GFF6566 at 5.268 kb on - strand, within GFF6566 at 5.268 kb on - strand, within GFF6566 at 5.268 kb on - strand, within GFF6566 at 5.287 kb on - strand, within GFF6566 at 5.358 kb on + strand, within GFF6566 at 5.358 kb on + strand, within GFF6566 at 5.358 kb on + strand, within GFF6566 at 5.359 kb on - strand, within GFF6566 at 5.359 kb on - strand, within GFF6566 at 5.395 kb on + strand, within GFF6566 at 5.539 kb on - strand, within GFF6566 at 5.539 kb on - strand, within GFF6566 at 5.574 kb on + strand, within GFF6566 at 5.574 kb on + strand, within GFF6566 at 5.575 kb on - strand, within GFF6566 at 5.601 kb on + strand, within GFF6566 at 5.896 kb on - strand, within GFF6566 at 5.898 kb on + strand, within GFF6566 at 5.898 kb on + strand, within GFF6566 at 5.898 kb on + strand, within GFF6566 at 5.898 kb on + strand, within GFF6566 at 5.899 kb on - strand, within GFF6566 at 5.899 kb on - strand, within GFF6566 at 5.940 kb on + strand, within GFF6566 at 6.189 kb on + strand, within GFF6566 at 6.193 kb on - strand, within GFF6566 at 6.283 kb on - strand, within GFF6566 at 6.379 kb on - strand, within GFF6566 at 6.457 kb on - strand, within GFF6566 at 6.885 kb on + strand, within GFF6566 at 6.885 kb on + strand, within GFF6566 at 6.886 kb on - strand, within GFF6566 at 6.987 kb on + strand, within GFF6566 at 6.987 kb on + strand, within GFF6566 at 6.988 kb on - strand, within GFF6566 at 7.209 kb on + strand, within GFF6566 at 7.210 kb on - strand, within GFF6566 at 7.234 kb on + strand, within GFF6566 at 7.234 kb on + strand, within GFF6566 at 7.234 kb on + strand, within GFF6566 at 7.234 kb on + strand, within GFF6566 at 7.235 kb on - strand, within GFF6566 at 7.235 kb on - strand, within GFF6566 at 7.235 kb on - strand, within GFF6566 at 7.281 kb on + strand, within GFF6566 at 7.281 kb on + strand, within GFF6566 at 7.312 kb on - strand, within GFF6566 at 7.381 kb on + strand at 7.382 kb on - strand at 7.625 kb on + strand at 7.625 kb on + strand at 7.625 kb on + strand at 7.625 kb on + strand at 7.626 kb on - strand at 7.626 kb on - strand at 7.626 kb on - strand at 7.647 kb on - strand at 7.647 kb on - strand at 7.647 kb on - strand at 7.715 kb on + strand at 7.715 kb on + strand at 7.715 kb on + strand at 7.715 kb on + strand at 7.715 kb on + strand at 7.715 kb on + strand at 7.715 kb on + strand at 7.715 kb on + strand at 7.715 kb on + strand at 7.715 kb on + strand at 7.715 kb on + strand at 7.716 kb on - strand at 7.716 kb on - strand at 7.716 kb on - strand at 7.716 kb on - strand at 7.716 kb on - strand at 7.716 kb on - strand at 7.716 kb on - strand at 7.716 kb on - strand at 7.716 kb on - strand at 7.772 kb on + strand, within GFF6567 at 7.773 kb on - strand, within GFF6567 at 8.171 kb on + strand, within GFF6567 at 8.172 kb on - strand, within GFF6567 at 8.172 kb on - strand, within GFF6567 at 8.172 kb on - strand, within GFF6567 at 8.195 kb on + strand, within GFF6567 at 8.195 kb on + strand, within GFF6567 at 8.196 kb on - strand, within GFF6567 at 8.196 kb on - strand, within GFF6567 at 8.196 kb on - strand, within GFF6567 at 8.196 kb on - strand, within GFF6567 at 8.324 kb on + strand, within GFF6567 at 8.324 kb on + strand, within GFF6567 at 8.324 kb on + strand, within GFF6567 at 8.324 kb on + strand, within GFF6567 at 8.324 kb on + strand, within GFF6567 at 8.324 kb on + strand, within GFF6567 at 8.324 kb on + strand, within GFF6567 at 8.324 kb on + strand, within GFF6567 at 8.324 kb on + strand, within GFF6567 at 8.324 kb on + strand, within GFF6567 at 8.324 kb on + strand, within GFF6567 at 8.325 kb on - strand, within GFF6567 at 8.325 kb on - strand, within GFF6567 at 8.325 kb on - strand, within GFF6567 at 8.325 kb on - strand, within GFF6567 at 8.325 kb on - strand, within GFF6567 at 8.325 kb on - strand, within GFF6567 at 8.325 kb on - strand, within GFF6567 at 8.325 kb on - strand, within GFF6567 at 8.325 kb on - strand, within GFF6567 at 8.325 kb on - strand, within GFF6567 at 8.360 kb on + strand, within GFF6567 at 8.361 kb on - strand, within GFF6567
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 4,150 - GFF6565 0.52 +0.7 4,150 - GFF6565 0.52 +1.4 4,166 - GFF6565 0.54 +0.2 4,245 + GFF6565 0.60 -0.1 4,246 - GFF6565 0.60 +0.2 4,246 - GFF6565 0.60 +0.4 4,246 - GFF6565 0.60 +0.1 4,246 - GFF6565 0.60 -0.4 4,260 - GFF6565 0.61 -0.9 4,260 - GFF6565 0.61 -0.7 4,260 - GFF6565 0.61 -0.4 4,426 + GFF6565 0.75 +0.2 4,427 - GFF6565 0.75 +0.1 4,669 - -0.1 4,799 + -1.1 4,820 + -0.3 4,870 - +0.3 4,923 + -0.4 4,924 - +0.8 4,981 - +0.5 5,033 + +0.3 5,034 - -0.5 5,034 - -0.0 5,196 + -0.4 5,197 - -1.3 5,197 - -0.4 5,241 + GFF6566 0.12 +0.3 5,244 + GFF6566 0.12 -0.6 5,267 + GFF6566 0.13 +0.7 5,267 + GFF6566 0.13 -2.1 5,268 - GFF6566 0.13 +0.5 5,268 - GFF6566 0.13 +0.6 5,268 - GFF6566 0.13 -0.5 5,287 - GFF6566 0.13 -1.5 5,358 + GFF6566 0.16 -0.7 5,358 + GFF6566 0.16 -0.7 5,358 + GFF6566 0.16 +0.1 5,359 - GFF6566 0.16 +0.3 5,359 - GFF6566 0.16 -0.8 5,395 + GFF6566 0.17 +2.0 5,539 - GFF6566 0.23 +0.4 5,539 - GFF6566 0.23 -1.6 5,574 + GFF6566 0.24 -0.2 5,574 + GFF6566 0.24 +0.5 5,575 - GFF6566 0.24 -0.4 5,601 + GFF6566 0.25 -1.6 5,896 - GFF6566 0.36 -1.1 5,898 + GFF6566 0.36 -0.1 5,898 + GFF6566 0.36 +0.6 5,898 + GFF6566 0.36 +0.2 5,898 + GFF6566 0.36 +0.5 5,899 - GFF6566 0.36 +0.1 5,899 - GFF6566 0.36 -2.2 5,940 + GFF6566 0.38 -0.7 6,189 + GFF6566 0.47 -0.2 6,193 - GFF6566 0.47 +1.9 6,283 - GFF6566 0.51 -0.4 6,379 - GFF6566 0.54 +0.3 6,457 - GFF6566 0.57 -0.5 6,885 + GFF6566 0.73 -1.6 6,885 + GFF6566 0.73 +0.2 6,886 - GFF6566 0.73 +1.8 6,987 + GFF6566 0.77 -0.2 6,987 + GFF6566 0.77 -1.2 6,988 - GFF6566 0.77 -0.2 7,209 + GFF6566 0.86 -1.9 7,210 - GFF6566 0.86 -0.8 7,234 + GFF6566 0.86 +0.2 7,234 + GFF6566 0.86 +2.0 7,234 + GFF6566 0.86 -0.3 7,234 + GFF6566 0.86 -1.9 7,235 - GFF6566 0.86 +0.4 7,235 - GFF6566 0.86 +0.1 7,235 - GFF6566 0.86 +0.3 7,281 + GFF6566 0.88 -0.1 7,281 + GFF6566 0.88 +0.1 7,312 - GFF6566 0.89 +1.1 7,381 + -1.0 7,382 - -1.0 7,625 + +0.2 7,625 + -0.8 7,625 + +0.6 7,625 + +0.5 7,626 - +0.2 7,626 - -0.4 7,626 - -0.3 7,647 - -1.9 7,647 - -0.3 7,647 - -0.1 7,715 + -0.4 7,715 + -0.9 7,715 + -0.6 7,715 + -0.5 7,715 + -0.4 7,715 + -0.5 7,715 + -0.8 7,715 + -2.4 7,715 + -0.5 7,715 + -0.3 7,715 + -0.3 7,716 - -0.1 7,716 - +0.5 7,716 - -0.6 7,716 - +0.4 7,716 - +0.9 7,716 - -1.1 7,716 - -1.2 7,716 - +1.4 7,716 - +0.4 7,772 + GFF6567 0.11 -0.2 7,773 - GFF6567 0.11 +0.7 8,171 + GFF6567 0.35 -0.2 8,172 - GFF6567 0.35 +0.4 8,172 - GFF6567 0.35 -0.6 8,172 - GFF6567 0.35 +0.0 8,195 + GFF6567 0.36 +0.0 8,195 + GFF6567 0.36 -0.1 8,196 - GFF6567 0.36 +0.4 8,196 - GFF6567 0.36 -0.1 8,196 - GFF6567 0.36 +0.1 8,196 - GFF6567 0.36 +0.0 8,324 + GFF6567 0.44 -0.3 8,324 + GFF6567 0.44 -0.6 8,324 + GFF6567 0.44 -0.9 8,324 + GFF6567 0.44 -0.2 8,324 + GFF6567 0.44 -1.0 8,324 + GFF6567 0.44 -0.9 8,324 + GFF6567 0.44 +0.2 8,324 + GFF6567 0.44 -1.1 8,324 + GFF6567 0.44 -0.6 8,324 + GFF6567 0.44 -0.3 8,324 + GFF6567 0.44 -0.1 8,325 - GFF6567 0.44 -0.2 8,325 - GFF6567 0.44 -0.2 8,325 - GFF6567 0.44 -0.1 8,325 - GFF6567 0.44 -0.6 8,325 - GFF6567 0.44 +1.4 8,325 - GFF6567 0.44 -0.2 8,325 - GFF6567 0.44 +0.9 8,325 - GFF6567 0.44 -0.1 8,325 - GFF6567 0.44 -0.5 8,325 - GFF6567 0.44 -0.5 8,360 + GFF6567 0.46 -1.2 8,361 - GFF6567 0.46 +0.3
Or see this region's nucleotide sequence