Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6533 and GFF6534 are separated by 6 nucleotides GFF6534 and GFF6535 are separated by 39 nucleotides GFF6535 and GFF6536 are separated by 13 nucleotides GFF6536 and GFF6537 are separated by 234 nucleotides
GFF6533 - Methylphosphotriester-DNA--protein-cysteine S-methyltransferase (EC 2.1.1.n11) / DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 29,188 to 30,774
GFF6533
GFF6534 - Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63), at 30,781 to 31,278
GFF6534
GFF6535 - Permease of the drug/metabolite transporter (DMT) superfamily, at 31,318 to 32,277
GFF6535
GFF6536 - protein of unknown function DUF1345, at 32,291 to 32,950
GFF6536
GFF6537 - hypothetical protein, at 33,185 to 34,024
GFF6537
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 30.547 kb on + strand, within GFF6533 at 30.547 kb on + strand, within GFF6533 at 30.547 kb on + strand, within GFF6533 at 30.547 kb on + strand, within GFF6533 at 30.548 kb on - strand, within GFF6533 at 30.548 kb on - strand, within GFF6533 at 30.548 kb on - strand, within GFF6533 at 30.548 kb on - strand, within GFF6533 at 30.679 kb on + strand at 30.679 kb on + strand at 30.680 kb on - strand at 30.680 kb on - strand at 30.680 kb on - strand at 30.680 kb on - strand at 30.680 kb on - strand at 30.805 kb on + strand at 30.805 kb on + strand at 30.805 kb on + strand at 30.805 kb on + strand at 30.805 kb on + strand at 30.805 kb on + strand at 30.805 kb on + strand at 30.805 kb on + strand at 30.805 kb on + strand at 30.806 kb on - strand at 30.806 kb on - strand at 30.806 kb on - strand at 30.806 kb on - strand at 30.806 kb on - strand at 31.090 kb on + strand, within GFF6534 at 31.090 kb on + strand, within GFF6534 at 31.090 kb on + strand, within GFF6534 at 31.090 kb on + strand, within GFF6534 at 31.091 kb on - strand, within GFF6534 at 31.091 kb on - strand, within GFF6534 at 31.277 kb on - strand at 31.277 kb on - strand at 31.870 kb on + strand, within GFF6535 at 31.871 kb on - strand, within GFF6535 at 32.062 kb on + strand, within GFF6535 at 32.062 kb on + strand, within GFF6535 at 32.062 kb on + strand, within GFF6535 at 32.062 kb on + strand, within GFF6535 at 32.062 kb on + strand, within GFF6535 at 32.062 kb on + strand, within GFF6535 at 32.063 kb on - strand, within GFF6535 at 32.063 kb on - strand, within GFF6535 at 32.063 kb on - strand, within GFF6535 at 32.063 kb on - strand, within GFF6535 at 32.063 kb on - strand, within GFF6535 at 32.442 kb on + strand, within GFF6536 at 32.442 kb on + strand, within GFF6536 at 32.442 kb on + strand, within GFF6536 at 32.443 kb on - strand, within GFF6536 at 32.443 kb on - strand, within GFF6536 at 32.443 kb on - strand, within GFF6536 at 32.443 kb on - strand, within GFF6536 at 32.443 kb on - strand, within GFF6536 at 32.443 kb on - strand, within GFF6536 at 32.443 kb on - strand, within GFF6536 at 32.448 kb on + strand, within GFF6536 at 32.448 kb on + strand, within GFF6536 at 32.448 kb on + strand, within GFF6536 at 32.449 kb on - strand, within GFF6536 at 32.449 kb on - strand, within GFF6536 at 32.449 kb on - strand, within GFF6536 at 32.449 kb on - strand, within GFF6536 at 32.449 kb on - strand, within GFF6536 at 32.449 kb on - strand, within GFF6536 at 32.449 kb on - strand, within GFF6536 at 32.451 kb on + strand, within GFF6536 at 32.535 kb on + strand, within GFF6536 at 32.535 kb on + strand, within GFF6536 at 32.535 kb on + strand, within GFF6536 at 32.535 kb on + strand, within GFF6536 at 32.535 kb on + strand, within GFF6536 at 32.535 kb on + strand, within GFF6536 at 32.536 kb on - strand, within GFF6536 at 32.536 kb on - strand, within GFF6536 at 32.536 kb on - strand, within GFF6536 at 32.536 kb on - strand, within GFF6536 at 32.538 kb on + strand, within GFF6536 at 32.539 kb on - strand, within GFF6536 at 32.563 kb on - strand, within GFF6536 at 32.729 kb on - strand, within GFF6536 at 32.904 kb on + strand at 32.904 kb on + strand at 32.904 kb on + strand at 32.961 kb on + strand at 32.961 kb on + strand at 32.962 kb on - strand at 33.023 kb on + strand at 33.024 kb on - strand at 33.050 kb on + strand at 33.050 kb on + strand at 33.050 kb on + strand at 33.050 kb on + strand at 33.050 kb on + strand at 33.050 kb on + strand at 33.050 kb on + strand at 33.051 kb on - strand at 33.051 kb on - strand at 33.051 kb on - strand at 33.051 kb on - strand at 33.051 kb on - strand at 33.069 kb on - strand at 33.072 kb on - strand at 33.157 kb on + strand at 33.158 kb on - strand at 33.158 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 30,547 + GFF6533 0.86 -0.3 30,547 + GFF6533 0.86 +0.3 30,547 + GFF6533 0.86 -0.5 30,547 + GFF6533 0.86 +0.3 30,548 - GFF6533 0.86 -0.7 30,548 - GFF6533 0.86 +1.2 30,548 - GFF6533 0.86 -0.9 30,548 - GFF6533 0.86 -0.5 30,679 + -0.2 30,679 + -0.2 30,680 - +0.7 30,680 - -0.1 30,680 - +0.1 30,680 - -0.2 30,680 - -0.1 30,805 + -1.2 30,805 + +2.0 30,805 + +0.6 30,805 + +0.9 30,805 + -1.3 30,805 + -0.3 30,805 + +0.5 30,805 + +1.5 30,805 + -1.0 30,806 - -0.4 30,806 - +0.3 30,806 - +0.2 30,806 - -0.2 30,806 - +1.0 31,090 + GFF6534 0.62 -0.3 31,090 + GFF6534 0.62 -0.6 31,090 + GFF6534 0.62 +0.2 31,090 + GFF6534 0.62 -0.5 31,091 - GFF6534 0.62 -0.3 31,091 - GFF6534 0.62 -0.5 31,277 - -0.7 31,277 - +0.3 31,870 + GFF6535 0.57 +1.0 31,871 - GFF6535 0.58 -0.2 32,062 + GFF6535 0.78 +0.1 32,062 + GFF6535 0.78 -1.8 32,062 + GFF6535 0.78 -0.6 32,062 + GFF6535 0.78 -0.8 32,062 + GFF6535 0.78 +0.2 32,062 + GFF6535 0.78 -0.8 32,063 - GFF6535 0.78 -0.9 32,063 - GFF6535 0.78 +0.3 32,063 - GFF6535 0.78 +0.7 32,063 - GFF6535 0.78 -0.3 32,063 - GFF6535 0.78 -1.3 32,442 + GFF6536 0.23 +0.0 32,442 + GFF6536 0.23 -0.2 32,442 + GFF6536 0.23 +1.0 32,443 - GFF6536 0.23 +0.8 32,443 - GFF6536 0.23 -1.3 32,443 - GFF6536 0.23 -0.1 32,443 - GFF6536 0.23 -0.0 32,443 - GFF6536 0.23 -0.1 32,443 - GFF6536 0.23 -0.9 32,443 - GFF6536 0.23 -0.1 32,448 + GFF6536 0.24 +0.8 32,448 + GFF6536 0.24 -0.5 32,448 + GFF6536 0.24 -0.1 32,449 - GFF6536 0.24 -0.5 32,449 - GFF6536 0.24 +0.2 32,449 - GFF6536 0.24 -0.0 32,449 - GFF6536 0.24 -0.3 32,449 - GFF6536 0.24 +0.2 32,449 - GFF6536 0.24 -0.5 32,449 - GFF6536 0.24 -0.3 32,451 + GFF6536 0.24 +0.4 32,535 + GFF6536 0.37 -0.0 32,535 + GFF6536 0.37 -0.3 32,535 + GFF6536 0.37 +1.5 32,535 + GFF6536 0.37 +0.4 32,535 + GFF6536 0.37 +0.0 32,535 + GFF6536 0.37 +0.3 32,536 - GFF6536 0.37 -1.0 32,536 - GFF6536 0.37 +0.0 32,536 - GFF6536 0.37 +1.0 32,536 - GFF6536 0.37 -0.5 32,538 + GFF6536 0.37 -0.3 32,539 - GFF6536 0.38 +2.0 32,563 - GFF6536 0.41 -0.2 32,729 - GFF6536 0.66 +0.8 32,904 + -0.1 32,904 + -1.3 32,904 + -1.0 32,961 + -0.1 32,961 + -0.1 32,962 - -0.3 33,023 + +0.1 33,024 - +0.8 33,050 + +0.5 33,050 + +0.1 33,050 + -0.1 33,050 + -0.3 33,050 + -0.2 33,050 + -1.8 33,050 + -0.3 33,051 - -0.9 33,051 - -0.3 33,051 - -0.5 33,051 - +0.1 33,051 - +0.1 33,069 - -1.0 33,072 - -0.5 33,157 + +1.1 33,158 - +0.1 33,158 - -1.5
Or see this region's nucleotide sequence