Strain Fitness in Variovorax sp. SCN45 around GFF6355

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6354 and GFF6355 are separated by 166 nucleotidesGFF6355 and GFF6356 are separated by 129 nucleotidesGFF6356 and GFF6357 are separated by 64 nucleotides GFF6354 - T6SS component TssK (ImpJ/VasE), at 19,435 to 20,811 GFF6354 GFF6355 - T6SS secretion lipoprotein TssJ (VasD), at 20,978 to 21,493 GFF6355 GFF6356 - putative lipoprotein, at 21,623 to 22,033 GFF6356 GFF6357 - T6SS forkhead associated domain protein ImpI/VasC, at 22,098 to 23,984 GFF6357 Position (kb) 20 21 22Strain fitness (log2 ratio) -2 -1 0 1at 20.039 kb on + strand, within GFF6354at 20.214 kb on - strand, within GFF6354at 20.214 kb on - strand, within GFF6354at 20.214 kb on - strand, within GFF6354at 20.617 kb on + strand, within GFF6354at 20.643 kb on - strand, within GFF6354at 20.678 kb on + strandat 20.678 kb on + strandat 20.678 kb on + strandat 20.690 kb on + strandat 20.691 kb on - strandat 20.726 kb on + strandat 20.726 kb on + strandat 20.727 kb on - strandat 20.727 kb on - strandat 20.727 kb on - strandat 20.902 kb on - strandat 20.915 kb on + strandat 20.942 kb on + strandat 20.943 kb on - strandat 20.959 kb on + strandat 20.959 kb on + strandat 20.962 kb on + strandat 20.980 kb on - strandat 20.990 kb on + strandat 20.991 kb on - strandat 21.064 kb on - strand, within GFF6355at 21.276 kb on + strand, within GFF6355at 21.521 kb on + strandat 21.521 kb on + strandat 21.523 kb on + strandat 21.523 kb on + strandat 21.612 kb on - strandat 21.771 kb on - strand, within GFF6356at 22.379 kb on - strand, within GFF6357at 22.386 kb on + strand, within GFF6357at 22.386 kb on + strand, within GFF6357

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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20,039 + GFF6354 0.44 -0.1
20,214 - GFF6354 0.57 -1.7
20,214 - GFF6354 0.57 -0.0
20,214 - GFF6354 0.57 -0.8
20,617 + GFF6354 0.86 +0.5
20,643 - GFF6354 0.88 +1.1
20,678 + +0.6
20,678 + -0.5
20,678 + +0.4
20,690 + +0.3
20,691 - +0.1
20,726 + -1.0
20,726 + -0.7
20,727 - -1.8
20,727 - -1.8
20,727 - -0.6
20,902 - +0.1
20,915 + -1.3
20,942 + -0.5
20,943 - -0.0
20,959 + -1.0
20,959 + +0.3
20,962 + +0.6
20,980 - -0.4
20,990 + +0.3
20,991 - -0.6
21,064 - GFF6355 0.17 -1.4
21,276 + GFF6355 0.58 -0.4
21,521 + -1.4
21,521 + -0.1
21,523 + -0.4
21,523 + -0.1
21,612 - +0.0
21,771 - GFF6356 0.36 +0.5
22,379 - GFF6357 0.15 -0.2
22,386 + GFF6357 0.15 -0.6
22,386 + GFF6357 0.15 -0.1

Or see this region's nucleotide sequence