Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6354 and GFF6355 are separated by 166 nucleotides GFF6355 and GFF6356 are separated by 129 nucleotides GFF6356 and GFF6357 are separated by 64 nucleotides
GFF6354 - T6SS component TssK (ImpJ/VasE), at 19,435 to 20,811
GFF6354
GFF6355 - T6SS secretion lipoprotein TssJ (VasD), at 20,978 to 21,493
GFF6355
GFF6356 - putative lipoprotein, at 21,623 to 22,033
GFF6356
GFF6357 - T6SS forkhead associated domain protein ImpI/VasC, at 22,098 to 23,984
GFF6357
Position (kb)
20
21
22 Strain fitness (log2 ratio)
-2
-1
0
1 at 20.039 kb on + strand, within GFF6354 at 20.214 kb on - strand, within GFF6354 at 20.214 kb on - strand, within GFF6354 at 20.214 kb on - strand, within GFF6354 at 20.617 kb on + strand, within GFF6354 at 20.643 kb on - strand, within GFF6354 at 20.678 kb on + strand at 20.678 kb on + strand at 20.678 kb on + strand at 20.690 kb on + strand at 20.691 kb on - strand at 20.726 kb on + strand at 20.726 kb on + strand at 20.727 kb on - strand at 20.727 kb on - strand at 20.727 kb on - strand at 20.902 kb on - strand at 20.915 kb on + strand at 20.942 kb on + strand at 20.943 kb on - strand at 20.959 kb on + strand at 20.959 kb on + strand at 20.962 kb on + strand at 20.980 kb on - strand at 20.990 kb on + strand at 20.991 kb on - strand at 21.064 kb on - strand, within GFF6355 at 21.276 kb on + strand, within GFF6355 at 21.521 kb on + strand at 21.521 kb on + strand at 21.523 kb on + strand at 21.523 kb on + strand at 21.612 kb on - strand at 21.771 kb on - strand, within GFF6356 at 22.379 kb on - strand, within GFF6357 at 22.386 kb on + strand, within GFF6357 at 22.386 kb on + strand, within GFF6357
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 20,039 + GFF6354 0.44 -0.1 20,214 - GFF6354 0.57 -1.7 20,214 - GFF6354 0.57 -0.0 20,214 - GFF6354 0.57 -0.8 20,617 + GFF6354 0.86 +0.5 20,643 - GFF6354 0.88 +1.1 20,678 + +0.6 20,678 + -0.5 20,678 + +0.4 20,690 + +0.3 20,691 - +0.1 20,726 + -1.0 20,726 + -0.7 20,727 - -1.8 20,727 - -1.8 20,727 - -0.6 20,902 - +0.1 20,915 + -1.3 20,942 + -0.5 20,943 - -0.0 20,959 + -1.0 20,959 + +0.3 20,962 + +0.6 20,980 - -0.4 20,990 + +0.3 20,991 - -0.6 21,064 - GFF6355 0.17 -1.4 21,276 + GFF6355 0.58 -0.4 21,521 + -1.4 21,521 + -0.1 21,523 + -0.4 21,523 + -0.1 21,612 - +0.0 21,771 - GFF6356 0.36 +0.5 22,379 - GFF6357 0.15 -0.2 22,386 + GFF6357 0.15 -0.6 22,386 + GFF6357 0.15 -0.1
Or see this region's nucleotide sequence