Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6298 and GFF6299 are separated by 51 nucleotides GFF6299 and GFF6300 are separated by 4 nucleotides
GFF6298 - Transcriptional regulator, LysR family, at 87,109 to 88,035
GFF6298
GFF6299 - BUG/TctC family periplasmic protein, at 88,087 to 89,067
GFF6299
GFF6300 - CaiB/BaiF family protein, at 89,072 to 90,262
GFF6300
Position (kb)
88
89
90 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 87.110 kb on + strand at 87.110 kb on + strand at 87.110 kb on + strand at 87.110 kb on + strand at 87.110 kb on + strand at 87.110 kb on + strand at 87.110 kb on + strand at 87.110 kb on + strand at 87.110 kb on + strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.111 kb on - strand at 87.144 kb on - strand at 87.144 kb on - strand at 87.215 kb on + strand, within GFF6298 at 87.215 kb on + strand, within GFF6298 at 87.215 kb on + strand, within GFF6298 at 87.215 kb on + strand, within GFF6298 at 87.215 kb on + strand, within GFF6298 at 87.215 kb on + strand, within GFF6298 at 87.216 kb on - strand, within GFF6298 at 87.216 kb on - strand, within GFF6298 at 87.216 kb on - strand, within GFF6298 at 87.216 kb on - strand, within GFF6298 at 87.216 kb on - strand, within GFF6298 at 87.418 kb on - strand, within GFF6298 at 87.418 kb on - strand, within GFF6298 at 87.418 kb on - strand, within GFF6298 at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.048 kb on + strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.049 kb on - strand at 88.050 kb on + strand at 88.050 kb on + strand at 88.051 kb on - strand at 88.051 kb on - strand at 88.051 kb on - strand at 88.051 kb on - strand at 88.088 kb on + strand at 88.089 kb on - strand at 88.089 kb on - strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.151 kb on + strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.152 kb on - strand at 88.331 kb on + strand, within GFF6299 at 88.331 kb on + strand, within GFF6299 at 88.331 kb on + strand, within GFF6299 at 88.331 kb on + strand, within GFF6299 at 88.331 kb on + strand, within GFF6299 at 88.331 kb on + strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.332 kb on - strand, within GFF6299 at 88.454 kb on + strand, within GFF6299 at 88.454 kb on + strand, within GFF6299 at 88.454 kb on + strand, within GFF6299 at 88.454 kb on + strand, within GFF6299 at 88.454 kb on + strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.455 kb on - strand, within GFF6299 at 88.505 kb on + strand, within GFF6299 at 88.505 kb on + strand, within GFF6299 at 88.505 kb on + strand, within GFF6299 at 88.505 kb on + strand, within GFF6299 at 88.505 kb on + strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.506 kb on - strand, within GFF6299 at 88.649 kb on + strand, within GFF6299 at 88.649 kb on + strand, within GFF6299 at 88.649 kb on + strand, within GFF6299 at 88.649 kb on + strand, within GFF6299 at 88.649 kb on + strand, within GFF6299 at 88.649 kb on + strand, within GFF6299 at 88.650 kb on - strand, within GFF6299 at 88.650 kb on - strand, within GFF6299 at 88.650 kb on - strand, within GFF6299 at 88.650 kb on - strand, within GFF6299 at 88.730 kb on + strand, within GFF6299 at 88.730 kb on + strand, within GFF6299 at 88.731 kb on - strand, within GFF6299 at 88.731 kb on - strand, within GFF6299 at 88.731 kb on - strand, within GFF6299 at 88.796 kb on + strand, within GFF6299 at 88.796 kb on + strand, within GFF6299 at 88.796 kb on + strand, within GFF6299 at 88.796 kb on + strand, within GFF6299 at 88.796 kb on + strand, within GFF6299 at 88.796 kb on + strand, within GFF6299 at 88.796 kb on + strand, within GFF6299 at 88.797 kb on - strand, within GFF6299 at 88.797 kb on - strand, within GFF6299 at 88.797 kb on - strand, within GFF6299 at 88.797 kb on - strand, within GFF6299 at 88.797 kb on - strand, within GFF6299 at 88.833 kb on + strand, within GFF6299 at 88.946 kb on + strand, within GFF6299 at 88.947 kb on - strand, within GFF6299 at 88.947 kb on - strand, within GFF6299 at 88.947 kb on - strand, within GFF6299 at 88.947 kb on - strand, within GFF6299 at 89.127 kb on + strand at 89.127 kb on + strand at 89.128 kb on - strand at 89.568 kb on + strand, within GFF6300 at 89.568 kb on + strand, within GFF6300 at 89.568 kb on + strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.569 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.818 kb on - strand, within GFF6300 at 89.871 kb on + strand, within GFF6300 at 89.871 kb on + strand, within GFF6300 at 89.871 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.904 kb on + strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.905 kb on - strand, within GFF6300 at 89.940 kb on + strand, within GFF6300 at 89.940 kb on + strand, within GFF6300 at 89.941 kb on - strand, within GFF6300 at 89.941 kb on - strand, within GFF6300 at 89.941 kb on - strand, within GFF6300
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 87,110 + +0.8 87,110 + -1.1 87,110 + +0.2 87,110 + +0.0 87,110 + -0.0 87,110 + -0.5 87,110 + -0.4 87,110 + -0.1 87,110 + -0.4 87,111 - -2.2 87,111 - +1.0 87,111 - +0.0 87,111 - +0.8 87,111 - -0.4 87,111 - +1.2 87,111 - -0.8 87,111 - +0.4 87,111 - +1.0 87,144 - +1.1 87,144 - +0.4 87,215 + GFF6298 0.11 -0.5 87,215 + GFF6298 0.11 +0.3 87,215 + GFF6298 0.11 -0.4 87,215 + GFF6298 0.11 -0.1 87,215 + GFF6298 0.11 -0.2 87,215 + GFF6298 0.11 -0.1 87,216 - GFF6298 0.12 -0.1 87,216 - GFF6298 0.12 -1.3 87,216 - GFF6298 0.12 -0.8 87,216 - GFF6298 0.12 -0.8 87,216 - GFF6298 0.12 +0.6 87,418 - GFF6298 0.33 +0.6 87,418 - GFF6298 0.33 -1.1 87,418 - GFF6298 0.33 +0.4 88,048 + -0.8 88,048 + -0.0 88,048 + +0.3 88,048 + +0.3 88,048 + -0.5 88,048 + -0.2 88,048 + -0.5 88,048 + -0.1 88,048 + +0.1 88,048 + -1.9 88,048 + -0.8 88,048 + -0.4 88,048 + -0.5 88,048 + +0.8 88,048 + -0.6 88,048 + +0.6 88,048 + -0.4 88,048 + -0.1 88,048 + -1.1 88,048 + -1.0 88,048 + +0.7 88,048 + +0.1 88,048 + -0.3 88,048 + -0.0 88,048 + +0.4 88,048 + -0.4 88,048 + -0.9 88,048 + -0.3 88,048 + +1.2 88,048 + -0.5 88,049 - -0.8 88,049 - -0.2 88,049 - +0.1 88,049 - -0.2 88,049 - -1.1 88,049 - -0.0 88,049 - +1.2 88,049 - -0.2 88,049 - +0.3 88,049 - -1.0 88,049 - +0.9 88,049 - -0.3 88,049 - -1.8 88,049 - -0.7 88,049 - -0.7 88,049 - +0.3 88,049 - -0.9 88,049 - +0.6 88,049 - +0.6 88,049 - -0.4 88,049 - +0.6 88,049 - -1.5 88,049 - +0.0 88,049 - -0.8 88,049 - -0.1 88,049 - -0.1 88,049 - -0.4 88,050 + +0.5 88,050 + +0.0 88,051 - -0.7 88,051 - +0.5 88,051 - +0.8 88,051 - -0.2 88,088 + +0.7 88,089 - -0.4 88,089 - +0.8 88,151 + -0.3 88,151 + -1.1 88,151 + -0.3 88,151 + -0.2 88,151 + +0.7 88,151 + -1.0 88,151 + +0.3 88,151 + -0.5 88,151 + +0.4 88,151 + -0.4 88,151 + -0.9 88,152 - -0.8 88,152 - -0.5 88,152 - -0.7 88,152 - +0.5 88,152 - -1.2 88,152 - -0.8 88,152 - -0.2 88,152 - +0.2 88,152 - +0.4 88,152 - -0.2 88,152 - +0.8 88,152 - -0.0 88,152 - -0.4 88,152 - +0.3 88,152 - -1.5 88,331 + GFF6299 0.25 +1.2 88,331 + GFF6299 0.25 -0.1 88,331 + GFF6299 0.25 +2.8 88,331 + GFF6299 0.25 -0.5 88,331 + GFF6299 0.25 +0.1 88,331 + GFF6299 0.25 -0.0 88,332 - GFF6299 0.25 -0.0 88,332 - GFF6299 0.25 -0.1 88,332 - GFF6299 0.25 -0.5 88,332 - GFF6299 0.25 +1.0 88,332 - GFF6299 0.25 +0.1 88,332 - GFF6299 0.25 -0.1 88,332 - GFF6299 0.25 -0.1 88,332 - GFF6299 0.25 +0.1 88,332 - GFF6299 0.25 -0.3 88,332 - GFF6299 0.25 +0.9 88,332 - GFF6299 0.25 -0.4 88,454 + GFF6299 0.37 -0.3 88,454 + GFF6299 0.37 -0.2 88,454 + GFF6299 0.37 +0.8 88,454 + GFF6299 0.37 +0.0 88,454 + GFF6299 0.37 +0.3 88,455 - GFF6299 0.38 -0.7 88,455 - GFF6299 0.38 -0.2 88,455 - GFF6299 0.38 +0.1 88,455 - GFF6299 0.38 +0.2 88,455 - GFF6299 0.38 +0.5 88,455 - GFF6299 0.38 -0.9 88,455 - GFF6299 0.38 -0.4 88,455 - GFF6299 0.38 +1.2 88,455 - GFF6299 0.38 +0.6 88,455 - GFF6299 0.38 -0.1 88,455 - GFF6299 0.38 -0.5 88,505 + GFF6299 0.43 -0.4 88,505 + GFF6299 0.43 -0.5 88,505 + GFF6299 0.43 -0.5 88,505 + GFF6299 0.43 +0.6 88,505 + GFF6299 0.43 +0.4 88,506 - GFF6299 0.43 +0.5 88,506 - GFF6299 0.43 -0.7 88,506 - GFF6299 0.43 -1.9 88,506 - GFF6299 0.43 -0.7 88,506 - GFF6299 0.43 -0.6 88,506 - GFF6299 0.43 -0.3 88,506 - GFF6299 0.43 -0.2 88,506 - GFF6299 0.43 -0.6 88,506 - GFF6299 0.43 +0.3 88,506 - GFF6299 0.43 -0.6 88,506 - GFF6299 0.43 +3.0 88,506 - GFF6299 0.43 +1.8 88,506 - GFF6299 0.43 -0.2 88,649 + GFF6299 0.57 -0.3 88,649 + GFF6299 0.57 -0.1 88,649 + GFF6299 0.57 -2.0 88,649 + GFF6299 0.57 -0.2 88,649 + GFF6299 0.57 +0.6 88,649 + GFF6299 0.57 -0.3 88,650 - GFF6299 0.57 -0.2 88,650 - GFF6299 0.57 -0.2 88,650 - GFF6299 0.57 +0.5 88,650 - GFF6299 0.57 +1.5 88,730 + GFF6299 0.66 -0.1 88,730 + GFF6299 0.66 -0.1 88,731 - GFF6299 0.66 -0.2 88,731 - GFF6299 0.66 +0.4 88,731 - GFF6299 0.66 +0.2 88,796 + GFF6299 0.72 -0.9 88,796 + GFF6299 0.72 +1.0 88,796 + GFF6299 0.72 +0.6 88,796 + GFF6299 0.72 -0.5 88,796 + GFF6299 0.72 -0.1 88,796 + GFF6299 0.72 +0.4 88,796 + GFF6299 0.72 -0.7 88,797 - GFF6299 0.72 -0.2 88,797 - GFF6299 0.72 -0.6 88,797 - GFF6299 0.72 -1.2 88,797 - GFF6299 0.72 +0.4 88,797 - GFF6299 0.72 -0.9 88,833 + GFF6299 0.76 -0.0 88,946 + GFF6299 0.88 -0.5 88,947 - GFF6299 0.88 -0.8 88,947 - GFF6299 0.88 +0.0 88,947 - GFF6299 0.88 +0.2 88,947 - GFF6299 0.88 +0.4 89,127 + +0.2 89,127 + -1.0 89,128 - +1.8 89,568 + GFF6300 0.42 -0.8 89,568 + GFF6300 0.42 -2.2 89,568 + GFF6300 0.42 +0.2 89,569 - GFF6300 0.42 +0.2 89,569 - GFF6300 0.42 -0.6 89,569 - GFF6300 0.42 -0.8 89,569 - GFF6300 0.42 +0.9 89,569 - GFF6300 0.42 -1.0 89,569 - GFF6300 0.42 +0.7 89,569 - GFF6300 0.42 -0.2 89,569 - GFF6300 0.42 +1.1 89,569 - GFF6300 0.42 +0.8 89,569 - GFF6300 0.42 -1.3 89,818 - GFF6300 0.63 -0.1 89,818 - GFF6300 0.63 -0.2 89,818 - GFF6300 0.63 -1.9 89,818 - GFF6300 0.63 -0.1 89,818 - GFF6300 0.63 +0.5 89,818 - GFF6300 0.63 -0.5 89,818 - GFF6300 0.63 +0.1 89,818 - GFF6300 0.63 +0.1 89,818 - GFF6300 0.63 -0.4 89,818 - GFF6300 0.63 -0.1 89,818 - GFF6300 0.63 +0.5 89,818 - GFF6300 0.63 +0.2 89,818 - GFF6300 0.63 -1.3 89,871 + GFF6300 0.67 -0.1 89,871 + GFF6300 0.67 -1.7 89,871 + GFF6300 0.67 +1.2 89,904 + GFF6300 0.70 +0.5 89,904 + GFF6300 0.70 +0.4 89,904 + GFF6300 0.70 +0.1 89,904 + GFF6300 0.70 -0.9 89,904 + GFF6300 0.70 +0.4 89,904 + GFF6300 0.70 +0.1 89,904 + GFF6300 0.70 -1.5 89,904 + GFF6300 0.70 -0.2 89,904 + GFF6300 0.70 -0.1 89,904 + GFF6300 0.70 +0.2 89,904 + GFF6300 0.70 -2.6 89,904 + GFF6300 0.70 +0.6 89,904 + GFF6300 0.70 -0.7 89,904 + GFF6300 0.70 -1.3 89,904 + GFF6300 0.70 -0.3 89,904 + GFF6300 0.70 +1.3 89,904 + GFF6300 0.70 -1.9 89,905 - GFF6300 0.70 -0.1 89,905 - GFF6300 0.70 -1.5 89,905 - GFF6300 0.70 -0.4 89,905 - GFF6300 0.70 -0.5 89,905 - GFF6300 0.70 +0.6 89,905 - GFF6300 0.70 +0.1 89,905 - GFF6300 0.70 -0.2 89,905 - GFF6300 0.70 +0.3 89,905 - GFF6300 0.70 +0.9 89,905 - GFF6300 0.70 +0.5 89,905 - GFF6300 0.70 -0.1 89,905 - GFF6300 0.70 -0.2 89,905 - GFF6300 0.70 -0.0 89,905 - GFF6300 0.70 -0.4 89,905 - GFF6300 0.70 -0.7 89,905 - GFF6300 0.70 -0.1 89,940 + GFF6300 0.73 -1.4 89,940 + GFF6300 0.73 +0.9 89,941 - GFF6300 0.73 -0.7 89,941 - GFF6300 0.73 +0.1 89,941 - GFF6300 0.73 -0.5
Or see this region's nucleotide sequence