Strain Fitness in Variovorax sp. SCN45 around GFF6262

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6260 and GFF6261 overlap by 4 nucleotidesGFF6261 and GFF6262 are separated by 77 nucleotidesGFF6262 and GFF6263 overlap by 8 nucleotidesGFF6263 and GFF6264 overlap by 4 nucleotides GFF6260 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnH (EC 2.7.8.37), at 53,448 to 54,077 GFF6260 GFF6261 - Alpha-D-ribose 1-methylphosphonate 5-triphosphate synthase subunit PhnG (EC 2.7.8.37), at 54,074 to 54,577 GFF6261 GFF6262 - Transcriptional regulator PhnF, at 54,655 to 55,410 GFF6262 GFF6263 - Uncharacterized protein Atu0170, clustered with phosphonate utilization, at 55,403 to 56,137 GFF6263 GFF6264 - Ribose 1,5-bisphosphate phosphokinase PhnN (EC 2.7.4.23), at 56,134 to 56,727 GFF6264 Position (kb) 54 55 56Strain fitness (log2 ratio) -2 -1 0 1 2at 54.303 kb on + strand, within GFF6261at 54.303 kb on + strand, within GFF6261at 54.304 kb on - strand, within GFF6261at 54.304 kb on - strand, within GFF6261at 54.586 kb on + strandat 54.587 kb on - strandat 54.587 kb on - strandat 54.587 kb on - strandat 54.603 kb on - strandat 54.632 kb on + strandat 54.632 kb on + strandat 54.632 kb on + strandat 54.632 kb on + strandat 54.632 kb on + strandat 54.633 kb on - strandat 54.633 kb on - strandat 54.633 kb on - strandat 54.633 kb on - strandat 54.633 kb on - strandat 54.633 kb on - strandat 54.633 kb on - strandat 54.636 kb on + strandat 54.636 kb on + strandat 54.637 kb on - strandat 54.637 kb on - strandat 54.763 kb on + strand, within GFF6262at 54.763 kb on + strand, within GFF6262at 54.764 kb on - strand, within GFF6262at 54.898 kb on + strand, within GFF6262at 54.898 kb on + strand, within GFF6262at 54.898 kb on + strand, within GFF6262at 54.898 kb on + strand, within GFF6262at 54.898 kb on + strand, within GFF6262at 54.899 kb on - strand, within GFF6262at 55.216 kb on + strand, within GFF6262at 55.216 kb on + strand, within GFF6262at 55.216 kb on + strand, within GFF6262at 55.216 kb on + strand, within GFF6262at 55.352 kb on - strandat 55.917 kb on - strand, within GFF6263at 56.152 kb on + strandat 56.152 kb on + strandat 56.153 kb on - strandat 56.153 kb on - strandat 56.153 kb on - strandat 56.368 kb on + strand, within GFF6264at 56.369 kb on - strand, within GFF6264

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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54,303 + GFF6261 0.45 +0.0
54,303 + GFF6261 0.45 +0.7
54,304 - GFF6261 0.46 +0.0
54,304 - GFF6261 0.46 -1.5
54,586 + -0.4
54,587 - +0.8
54,587 - -0.5
54,587 - +0.0
54,603 - +0.4
54,632 + +0.4
54,632 + -0.2
54,632 + -0.5
54,632 + -0.1
54,632 + +1.8
54,633 - -0.2
54,633 - -0.2
54,633 - -0.5
54,633 - -0.3
54,633 - -0.0
54,633 - -0.4
54,633 - -0.3
54,636 + -0.2
54,636 + +0.2
54,637 - -0.1
54,637 - -0.6
54,763 + GFF6262 0.14 -0.4
54,763 + GFF6262 0.14 -0.5
54,764 - GFF6262 0.14 -1.5
54,898 + GFF6262 0.32 -0.4
54,898 + GFF6262 0.32 +0.8
54,898 + GFF6262 0.32 -1.0
54,898 + GFF6262 0.32 -0.7
54,898 + GFF6262 0.32 +0.7
54,899 - GFF6262 0.32 +0.3
55,216 + GFF6262 0.74 -0.1
55,216 + GFF6262 0.74 -2.7
55,216 + GFF6262 0.74 +0.2
55,216 + GFF6262 0.74 -1.8
55,352 - +1.0
55,917 - GFF6263 0.70 -0.3
56,152 + -0.2
56,152 + -0.2
56,153 - +0.6
56,153 - -1.8
56,153 - -0.5
56,368 + GFF6264 0.39 -0.5
56,369 - GFF6264 0.40 -0.5

Or see this region's nucleotide sequence