Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6250 and GFF6251 are separated by 19 nucleotides GFF6251 and GFF6252 are separated by 10 nucleotides GFF6252 and GFF6253 are separated by 31 nucleotides
GFF6250 - Glutamate--cysteine ligase-like protein YbdK, at 44,210 to 45,430
GFF6250
GFF6251 - hypothetical protein, at 45,450 to 46,019
GFF6251
GFF6252 - Phosphonate ABC transporter permease protein PhnE (TC 3.A.1.9.1), at 46,030 to 46,833
GFF6252
GFF6253 - Phosphonate ABC transporter substrate-binding protein PhnD (TC 3.A.1.9.1), at 46,865 to 47,815
GFF6253
Position (kb) 45
46
47 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 44.639 kb on + strand, within GFF6250 at 44.639 kb on + strand, within GFF6250 at 44.639 kb on + strand, within GFF6250 at 44.639 kb on + strand, within GFF6250 at 44.640 kb on - strand, within GFF6250 at 44.675 kb on + strand, within GFF6250 at 44.675 kb on + strand, within GFF6250 at 44.676 kb on - strand, within GFF6250 at 44.676 kb on - strand, within GFF6250 at 44.676 kb on - strand, within GFF6250 at 44.676 kb on - strand, within GFF6250 at 44.778 kb on - strand, within GFF6250 at 44.778 kb on - strand, within GFF6250 at 44.778 kb on - strand, within GFF6250 at 44.781 kb on + strand, within GFF6250 at 44.781 kb on + strand, within GFF6250 at 44.781 kb on + strand, within GFF6250 at 44.781 kb on + strand, within GFF6250 at 44.782 kb on - strand, within GFF6250 at 44.928 kb on - strand, within GFF6250 at 44.928 kb on - strand, within GFF6250 at 45.074 kb on + strand, within GFF6250 at 45.074 kb on + strand, within GFF6250 at 45.074 kb on + strand, within GFF6250 at 45.074 kb on + strand, within GFF6250 at 45.075 kb on - strand, within GFF6250 at 45.075 kb on - strand, within GFF6250 at 45.075 kb on - strand, within GFF6250 at 45.075 kb on - strand, within GFF6250 at 45.137 kb on + strand, within GFF6250 at 45.147 kb on - strand, within GFF6250 at 45.152 kb on + strand, within GFF6250 at 45.152 kb on + strand, within GFF6250 at 45.152 kb on + strand, within GFF6250 at 45.152 kb on + strand, within GFF6250 at 45.152 kb on + strand, within GFF6250 at 45.153 kb on - strand, within GFF6250 at 45.153 kb on - strand, within GFF6250 at 45.153 kb on - strand, within GFF6250 at 45.153 kb on - strand, within GFF6250 at 45.153 kb on - strand, within GFF6250 at 45.194 kb on + strand, within GFF6250 at 45.194 kb on + strand, within GFF6250 at 45.195 kb on - strand, within GFF6250 at 45.493 kb on + strand at 45.494 kb on - strand at 45.494 kb on - strand at 45.545 kb on - strand, within GFF6251 at 45.775 kb on + strand, within GFF6251 at 45.775 kb on + strand, within GFF6251 at 45.775 kb on + strand, within GFF6251 at 45.776 kb on - strand, within GFF6251 at 45.776 kb on - strand, within GFF6251 at 46.031 kb on + strand at 46.031 kb on + strand at 46.032 kb on - strand at 46.032 kb on - strand at 46.616 kb on + strand, within GFF6252 at 46.616 kb on + strand, within GFF6252 at 46.617 kb on - strand, within GFF6252 at 46.617 kb on - strand, within GFF6252
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 44,639 + GFF6250 0.35 -1.5 44,639 + GFF6250 0.35 -2.0 44,639 + GFF6250 0.35 -2.9 44,639 + GFF6250 0.35 -1.1 44,640 - GFF6250 0.35 -0.6 44,675 + GFF6250 0.38 -1.9 44,675 + GFF6250 0.38 -1.6 44,676 - GFF6250 0.38 +0.4 44,676 - GFF6250 0.38 -1.7 44,676 - GFF6250 0.38 -0.4 44,676 - GFF6250 0.38 -2.2 44,778 - GFF6250 0.47 -3.7 44,778 - GFF6250 0.47 -1.4 44,778 - GFF6250 0.47 -0.8 44,781 + GFF6250 0.47 -1.8 44,781 + GFF6250 0.47 -1.5 44,781 + GFF6250 0.47 -0.9 44,781 + GFF6250 0.47 -2.1 44,782 - GFF6250 0.47 -0.8 44,928 - GFF6250 0.59 -0.3 44,928 - GFF6250 0.59 -2.4 45,074 + GFF6250 0.71 -1.0 45,074 + GFF6250 0.71 -1.7 45,074 + GFF6250 0.71 -1.7 45,074 + GFF6250 0.71 -1.1 45,075 - GFF6250 0.71 -1.8 45,075 - GFF6250 0.71 -1.5 45,075 - GFF6250 0.71 -0.4 45,075 - GFF6250 0.71 -1.8 45,137 + GFF6250 0.76 -1.6 45,147 - GFF6250 0.77 -2.2 45,152 + GFF6250 0.77 -2.1 45,152 + GFF6250 0.77 -0.8 45,152 + GFF6250 0.77 -2.1 45,152 + GFF6250 0.77 -0.8 45,152 + GFF6250 0.77 -2.1 45,153 - GFF6250 0.77 +2.8 45,153 - GFF6250 0.77 -0.9 45,153 - GFF6250 0.77 -1.4 45,153 - GFF6250 0.77 -1.2 45,153 - GFF6250 0.77 -2.2 45,194 + GFF6250 0.81 -0.5 45,194 + GFF6250 0.81 -1.5 45,195 - GFF6250 0.81 -0.2 45,493 + +0.4 45,494 - +0.1 45,494 - +0.3 45,545 - GFF6251 0.17 +0.2 45,775 + GFF6251 0.57 -0.0 45,775 + GFF6251 0.57 +0.5 45,775 + GFF6251 0.57 +0.3 45,776 - GFF6251 0.57 -0.1 45,776 - GFF6251 0.57 +0.4 46,031 + -1.1 46,031 + +1.0 46,032 - -0.5 46,032 - -0.6 46,616 + GFF6252 0.73 +0.3 46,616 + GFF6252 0.73 +0.8 46,617 - GFF6252 0.73 -1.0 46,617 - GFF6252 0.73 +1.4
Or see this region's nucleotide sequence