Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF621 and GFF622 overlap by 4 nucleotides GFF622 and GFF623 overlap by 4 nucleotides GFF623 and GFF624 are separated by 76 nucleotides GFF624 and GFF625 are separated by 175 nucleotides
GFF621 - Aspartate aminotransferase (EC 2.6.1.1), at 668,367 to 669,545
GFF621
GFF622 - BUG/TctC family periplasmic protein, at 669,542 to 670,528
GFF622
GFF623 - Demethylmenaquinone methyltransferase-like protein, at 670,525 to 671,253
GFF623
GFF624 - hypothetical protein, at 671,330 to 671,680
GFF624
GFF625 - Efflux transport system, outer membrane factor (OMF) lipoprotein, at 671,856 to 673,421
GFF625
Position (kb) 670
671
672 Strain fitness (log2 ratio) -1
0
1 at 669.932 kb on + strand, within GFF622 at 669.933 kb on - strand, within GFF622 at 669.933 kb on - strand, within GFF622 at 669.933 kb on - strand, within GFF622 at 669.933 kb on - strand, within GFF622 at 669.989 kb on + strand, within GFF622 at 669.990 kb on - strand, within GFF622 at 670.106 kb on + strand, within GFF622 at 670.107 kb on - strand, within GFF622 at 670.107 kb on - strand, within GFF622 at 670.107 kb on - strand, within GFF622 at 670.107 kb on - strand, within GFF622 at 670.107 kb on - strand, within GFF622 at 671.090 kb on + strand, within GFF623 at 671.091 kb on - strand, within GFF623 at 671.331 kb on + strand at 671.331 kb on + strand at 671.331 kb on + strand at 671.331 kb on + strand at 671.331 kb on + strand at 671.331 kb on + strand at 671.332 kb on - strand at 671.332 kb on - strand at 671.332 kb on - strand at 671.374 kb on - strand, within GFF624 at 671.520 kb on + strand, within GFF624 at 671.607 kb on + strand, within GFF624 at 672.019 kb on + strand, within GFF625 at 672.019 kb on + strand, within GFF625 at 672.020 kb on - strand, within GFF625 at 672.020 kb on - strand, within GFF625 at 672.020 kb on - strand, within GFF625
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 669,932 + GFF622 0.40 -0.9 669,933 - GFF622 0.40 +0.3 669,933 - GFF622 0.40 +0.7 669,933 - GFF622 0.40 -0.5 669,933 - GFF622 0.40 -1.2 669,989 + GFF622 0.45 -0.2 669,990 - GFF622 0.45 +0.0 670,106 + GFF622 0.57 -0.4 670,107 - GFF622 0.57 -0.3 670,107 - GFF622 0.57 -0.0 670,107 - GFF622 0.57 -0.6 670,107 - GFF622 0.57 +0.8 670,107 - GFF622 0.57 -0.2 671,090 + GFF623 0.78 -0.2 671,091 - GFF623 0.78 +0.6 671,331 + -0.6 671,331 + -0.0 671,331 + -0.6 671,331 + -0.4 671,331 + -0.2 671,331 + -1.1 671,332 - +0.7 671,332 - -0.7 671,332 - -0.0 671,374 - GFF624 0.13 -0.0 671,520 + GFF624 0.54 -0.6 671,607 + GFF624 0.79 +0.6 672,019 + GFF625 0.10 -0.2 672,019 + GFF625 0.10 -0.1 672,020 - GFF625 0.10 +0.5 672,020 - GFF625 0.10 -1.0 672,020 - GFF625 0.10 +1.5
Or see this region's nucleotide sequence