Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6076 and GFF6077 overlap by 1 nucleotides GFF6077 and GFF6078 are separated by 39 nucleotides GFF6078 and GFF6079 are separated by 163 nucleotides GFF6079 and GFF6080 are separated by 131 nucleotides
GFF6076 - Spermidine synthase-like protein, at 139,214 to 139,999
GFF6076
GFF6077 - G:T/U mismatch-specific uracil/thymine DNA-glycosylase (EC 3.2.2.27), at 139,999 to 140,532
GFF6077
GFF6078 - no description, at 140,572 to 141,393
GFF6078
GFF6079 - hypothetical protein, at 141,557 to 141,982
GFF6079
GFF6080 - Putative deoxyribonuclease YjjV, at 142,114 to 142,974
GFF6080
Position (kb)
140
141
142 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 139.860 kb on + strand, within GFF6076 at 139.860 kb on + strand, within GFF6076 at 139.860 kb on + strand, within GFF6076 at 139.860 kb on + strand, within GFF6076 at 139.861 kb on - strand, within GFF6076 at 139.861 kb on - strand, within GFF6076 at 139.861 kb on - strand, within GFF6076 at 139.933 kb on - strand at 140.008 kb on + strand at 140.009 kb on - strand at 140.009 kb on - strand at 140.099 kb on + strand, within GFF6077 at 140.100 kb on - strand, within GFF6077 at 140.100 kb on - strand, within GFF6077 at 140.261 kb on + strand, within GFF6077 at 140.261 kb on + strand, within GFF6077 at 140.261 kb on + strand, within GFF6077 at 140.261 kb on + strand, within GFF6077 at 140.261 kb on + strand, within GFF6077 at 140.261 kb on + strand, within GFF6077 at 140.261 kb on + strand, within GFF6077 at 140.261 kb on + strand, within GFF6077 at 140.262 kb on - strand, within GFF6077 at 140.262 kb on - strand, within GFF6077 at 140.262 kb on - strand, within GFF6077 at 140.262 kb on - strand, within GFF6077 at 140.262 kb on - strand, within GFF6077 at 140.262 kb on - strand, within GFF6077 at 140.262 kb on - strand, within GFF6077 at 140.262 kb on - strand, within GFF6077 at 140.262 kb on - strand, within GFF6077 at 140.262 kb on - strand, within GFF6077 at 140.262 kb on - strand, within GFF6077 at 140.262 kb on - strand, within GFF6077 at 140.315 kb on + strand, within GFF6077 at 140.315 kb on + strand, within GFF6077 at 140.315 kb on + strand, within GFF6077 at 140.316 kb on - strand, within GFF6077 at 140.316 kb on - strand, within GFF6077 at 140.316 kb on - strand, within GFF6077 at 140.316 kb on - strand, within GFF6077 at 140.396 kb on + strand, within GFF6077 at 140.396 kb on + strand, within GFF6077 at 140.396 kb on + strand, within GFF6077 at 140.396 kb on + strand, within GFF6077 at 140.396 kb on + strand, within GFF6077 at 140.396 kb on + strand, within GFF6077 at 140.396 kb on + strand, within GFF6077 at 140.396 kb on + strand, within GFF6077 at 140.396 kb on + strand, within GFF6077 at 140.397 kb on - strand, within GFF6077 at 140.397 kb on - strand, within GFF6077 at 140.397 kb on - strand, within GFF6077 at 140.397 kb on - strand, within GFF6077 at 140.397 kb on - strand, within GFF6077 at 140.399 kb on + strand, within GFF6077 at 140.399 kb on + strand, within GFF6077 at 140.399 kb on + strand, within GFF6077 at 140.399 kb on + strand, within GFF6077 at 140.399 kb on + strand, within GFF6077 at 140.399 kb on + strand, within GFF6077 at 140.399 kb on + strand, within GFF6077 at 140.399 kb on + strand, within GFF6077 at 140.399 kb on + strand, within GFF6077 at 140.400 kb on - strand, within GFF6077 at 140.400 kb on - strand, within GFF6077 at 140.400 kb on - strand, within GFF6077 at 140.400 kb on - strand, within GFF6077 at 140.400 kb on - strand, within GFF6077 at 140.400 kb on - strand, within GFF6077 at 140.400 kb on - strand, within GFF6077 at 140.400 kb on - strand, within GFF6077 at 140.400 kb on - strand, within GFF6077 at 140.400 kb on - strand, within GFF6077 at 140.400 kb on - strand, within GFF6077 at 140.400 kb on - strand, within GFF6077 at 140.449 kb on + strand, within GFF6077 at 140.450 kb on - strand, within GFF6077 at 140.450 kb on - strand, within GFF6077 at 140.450 kb on - strand, within GFF6077 at 140.585 kb on + strand at 140.585 kb on + strand at 140.585 kb on + strand at 140.585 kb on + strand at 140.585 kb on + strand at 140.586 kb on - strand at 140.586 kb on - strand at 140.586 kb on - strand at 140.586 kb on - strand at 140.586 kb on - strand at 140.586 kb on - strand at 140.675 kb on + strand, within GFF6078 at 140.718 kb on - strand, within GFF6078 at 140.768 kb on + strand, within GFF6078 at 140.768 kb on + strand, within GFF6078 at 140.768 kb on + strand, within GFF6078 at 140.768 kb on + strand, within GFF6078 at 140.768 kb on + strand, within GFF6078 at 140.768 kb on + strand, within GFF6078 at 140.768 kb on + strand, within GFF6078 at 140.768 kb on + strand, within GFF6078 at 140.768 kb on + strand, within GFF6078 at 140.769 kb on - strand, within GFF6078 at 140.769 kb on - strand, within GFF6078 at 140.769 kb on - strand, within GFF6078 at 140.769 kb on - strand, within GFF6078 at 140.769 kb on - strand, within GFF6078 at 140.769 kb on - strand, within GFF6078 at 140.808 kb on + strand, within GFF6078 at 140.867 kb on + strand, within GFF6078 at 141.048 kb on - strand, within GFF6078 at 141.057 kb on - strand, within GFF6078 at 141.062 kb on + strand, within GFF6078 at 141.062 kb on + strand, within GFF6078 at 141.062 kb on + strand, within GFF6078 at 141.062 kb on + strand, within GFF6078 at 141.062 kb on + strand, within GFF6078 at 141.062 kb on + strand, within GFF6078 at 141.063 kb on - strand, within GFF6078 at 141.063 kb on - strand, within GFF6078 at 141.063 kb on - strand, within GFF6078 at 141.063 kb on - strand, within GFF6078 at 141.063 kb on - strand, within GFF6078 at 141.063 kb on - strand, within GFF6078 at 141.063 kb on - strand, within GFF6078 at 141.063 kb on - strand, within GFF6078 at 141.063 kb on - strand, within GFF6078 at 141.063 kb on - strand, within GFF6078 at 141.155 kb on + strand, within GFF6078 at 141.155 kb on + strand, within GFF6078 at 141.156 kb on - strand, within GFF6078 at 141.275 kb on + strand, within GFF6078 at 141.275 kb on + strand, within GFF6078 at 141.276 kb on - strand, within GFF6078 at 141.276 kb on - strand, within GFF6078 at 141.276 kb on - strand, within GFF6078 at 141.393 kb on + strand at 141.393 kb on + strand at 141.394 kb on - strand at 141.437 kb on + strand at 141.437 kb on + strand at 141.437 kb on + strand at 141.437 kb on + strand at 141.437 kb on + strand at 141.437 kb on + strand at 141.437 kb on + strand at 141.437 kb on + strand at 141.437 kb on + strand at 141.437 kb on + strand at 141.438 kb on - strand at 141.438 kb on - strand at 141.438 kb on - strand at 141.438 kb on - strand at 141.438 kb on - strand at 141.438 kb on - strand at 141.438 kb on - strand at 141.438 kb on - strand at 141.438 kb on - strand at 141.544 kb on + strand at 141.544 kb on + strand at 141.545 kb on - strand at 141.545 kb on - strand at 141.545 kb on - strand at 141.545 kb on - strand at 141.555 kb on - strand at 141.558 kb on + strand at 141.558 kb on + strand at 141.558 kb on + strand at 141.558 kb on + strand at 141.558 kb on + strand at 141.558 kb on + strand at 141.558 kb on + strand at 141.558 kb on + strand at 141.558 kb on + strand at 141.558 kb on + strand at 141.558 kb on + strand at 141.559 kb on - strand at 141.559 kb on - strand at 141.559 kb on - strand at 141.559 kb on - strand at 141.559 kb on - strand at 141.559 kb on - strand at 141.559 kb on - strand at 141.559 kb on - strand at 141.801 kb on + strand, within GFF6079 at 141.801 kb on + strand, within GFF6079 at 141.801 kb on + strand, within GFF6079 at 141.802 kb on - strand, within GFF6079 at 141.802 kb on - strand, within GFF6079 at 141.802 kb on - strand, within GFF6079 at 141.802 kb on - strand, within GFF6079 at 141.802 kb on - strand, within GFF6079 at 142.009 kb on + strand at 142.009 kb on + strand at 142.035 kb on + strand at 142.035 kb on + strand at 142.036 kb on - strand at 142.047 kb on - strand at 142.047 kb on - strand at 142.115 kb on + strand at 142.115 kb on + strand at 142.115 kb on + strand at 142.116 kb on - strand at 142.159 kb on + strand at 142.319 kb on + strand, within GFF6080 at 142.320 kb on - strand, within GFF6080
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 139,860 + GFF6076 0.82 -1.8 139,860 + GFF6076 0.82 -1.5 139,860 + GFF6076 0.82 -0.8 139,860 + GFF6076 0.82 -0.1 139,861 - GFF6076 0.82 -1.0 139,861 - GFF6076 0.82 -0.8 139,861 - GFF6076 0.82 +0.1 139,933 - +0.0 140,008 + +0.0 140,009 - +0.2 140,009 - -0.0 140,099 + GFF6077 0.19 +0.7 140,100 - GFF6077 0.19 -1.0 140,100 - GFF6077 0.19 +0.7 140,261 + GFF6077 0.49 -0.5 140,261 + GFF6077 0.49 +0.8 140,261 + GFF6077 0.49 +0.6 140,261 + GFF6077 0.49 -0.4 140,261 + GFF6077 0.49 -1.8 140,261 + GFF6077 0.49 +0.3 140,261 + GFF6077 0.49 +0.7 140,261 + GFF6077 0.49 +0.4 140,262 - GFF6077 0.49 -0.9 140,262 - GFF6077 0.49 -0.8 140,262 - GFF6077 0.49 -1.8 140,262 - GFF6077 0.49 +1.6 140,262 - GFF6077 0.49 -1.2 140,262 - GFF6077 0.49 +0.5 140,262 - GFF6077 0.49 -0.6 140,262 - GFF6077 0.49 -0.5 140,262 - GFF6077 0.49 -0.2 140,262 - GFF6077 0.49 +0.7 140,262 - GFF6077 0.49 +0.5 140,262 - GFF6077 0.49 -0.5 140,315 + GFF6077 0.59 +1.2 140,315 + GFF6077 0.59 -1.3 140,315 + GFF6077 0.59 -0.4 140,316 - GFF6077 0.59 +0.1 140,316 - GFF6077 0.59 -0.7 140,316 - GFF6077 0.59 -0.2 140,316 - GFF6077 0.59 -0.7 140,396 + GFF6077 0.74 -0.3 140,396 + GFF6077 0.74 +0.3 140,396 + GFF6077 0.74 -2.0 140,396 + GFF6077 0.74 -1.7 140,396 + GFF6077 0.74 -0.6 140,396 + GFF6077 0.74 -0.5 140,396 + GFF6077 0.74 -0.1 140,396 + GFF6077 0.74 +1.4 140,396 + GFF6077 0.74 -0.7 140,397 - GFF6077 0.75 -0.1 140,397 - GFF6077 0.75 +0.2 140,397 - GFF6077 0.75 -0.1 140,397 - GFF6077 0.75 +0.2 140,397 - GFF6077 0.75 -1.2 140,399 + GFF6077 0.75 -1.3 140,399 + GFF6077 0.75 -0.4 140,399 + GFF6077 0.75 +0.4 140,399 + GFF6077 0.75 -0.4 140,399 + GFF6077 0.75 -1.7 140,399 + GFF6077 0.75 +0.7 140,399 + GFF6077 0.75 -1.9 140,399 + GFF6077 0.75 +0.2 140,399 + GFF6077 0.75 -0.4 140,400 - GFF6077 0.75 +0.1 140,400 - GFF6077 0.75 -0.2 140,400 - GFF6077 0.75 -0.3 140,400 - GFF6077 0.75 +0.4 140,400 - GFF6077 0.75 -0.3 140,400 - GFF6077 0.75 +0.0 140,400 - GFF6077 0.75 -0.2 140,400 - GFF6077 0.75 -0.7 140,400 - GFF6077 0.75 -0.3 140,400 - GFF6077 0.75 -0.2 140,400 - GFF6077 0.75 +0.0 140,400 - GFF6077 0.75 -0.4 140,449 + GFF6077 0.84 -0.4 140,450 - GFF6077 0.84 +0.9 140,450 - GFF6077 0.84 +0.1 140,450 - GFF6077 0.84 -0.7 140,585 + +0.0 140,585 + +0.9 140,585 + -0.8 140,585 + -0.3 140,585 + -1.6 140,586 - +0.0 140,586 - -0.2 140,586 - -0.2 140,586 - +0.1 140,586 - -0.3 140,586 - -0.9 140,675 + GFF6078 0.13 -1.2 140,718 - GFF6078 0.18 +0.3 140,768 + GFF6078 0.24 -1.0 140,768 + GFF6078 0.24 +0.0 140,768 + GFF6078 0.24 +0.5 140,768 + GFF6078 0.24 +0.1 140,768 + GFF6078 0.24 -0.4 140,768 + GFF6078 0.24 +0.5 140,768 + GFF6078 0.24 -1.0 140,768 + GFF6078 0.24 +0.5 140,768 + GFF6078 0.24 -0.0 140,769 - GFF6078 0.24 +0.4 140,769 - GFF6078 0.24 -0.3 140,769 - GFF6078 0.24 -0.1 140,769 - GFF6078 0.24 +0.0 140,769 - GFF6078 0.24 +0.2 140,769 - GFF6078 0.24 +0.3 140,808 + GFF6078 0.29 +0.3 140,867 + GFF6078 0.36 -0.7 141,048 - GFF6078 0.58 -0.1 141,057 - GFF6078 0.59 -0.7 141,062 + GFF6078 0.60 -0.8 141,062 + GFF6078 0.60 -0.0 141,062 + GFF6078 0.60 -0.2 141,062 + GFF6078 0.60 +1.1 141,062 + GFF6078 0.60 -0.3 141,062 + GFF6078 0.60 -0.6 141,063 - GFF6078 0.60 -0.6 141,063 - GFF6078 0.60 -0.4 141,063 - GFF6078 0.60 -0.2 141,063 - GFF6078 0.60 +1.6 141,063 - GFF6078 0.60 +0.7 141,063 - GFF6078 0.60 +0.2 141,063 - GFF6078 0.60 +0.2 141,063 - GFF6078 0.60 -0.9 141,063 - GFF6078 0.60 +1.1 141,063 - GFF6078 0.60 -0.2 141,155 + GFF6078 0.71 -0.7 141,155 + GFF6078 0.71 +0.4 141,156 - GFF6078 0.71 -0.5 141,275 + GFF6078 0.86 +0.8 141,275 + GFF6078 0.86 -0.9 141,276 - GFF6078 0.86 -0.4 141,276 - GFF6078 0.86 +1.0 141,276 - GFF6078 0.86 +1.0 141,393 + -0.3 141,393 + -0.4 141,394 - +1.9 141,437 + -0.3 141,437 + -1.0 141,437 + +0.4 141,437 + +0.5 141,437 + -0.1 141,437 + -0.7 141,437 + +0.2 141,437 + +0.4 141,437 + -0.4 141,437 + +1.6 141,438 - +0.6 141,438 - +0.5 141,438 - -1.5 141,438 - -0.6 141,438 - +0.7 141,438 - -0.0 141,438 - +2.0 141,438 - +0.2 141,438 - -0.2 141,544 + -0.9 141,544 + -0.7 141,545 - -0.3 141,545 - -0.2 141,545 - -0.1 141,545 - -0.8 141,555 - -1.4 141,558 + +0.1 141,558 + +0.1 141,558 + -0.5 141,558 + -0.8 141,558 + -0.4 141,558 + -0.1 141,558 + +2.0 141,558 + -0.1 141,558 + +1.0 141,558 + -0.0 141,558 + -1.7 141,559 - -0.1 141,559 - -0.2 141,559 - -0.2 141,559 - +0.7 141,559 - +0.1 141,559 - +1.6 141,559 - -2.0 141,559 - -0.7 141,801 + GFF6079 0.57 +0.4 141,801 + GFF6079 0.57 +0.0 141,801 + GFF6079 0.57 -1.9 141,802 - GFF6079 0.58 -0.7 141,802 - GFF6079 0.58 -0.8 141,802 - GFF6079 0.58 -0.6 141,802 - GFF6079 0.58 -0.6 141,802 - GFF6079 0.58 -0.3 142,009 + -0.7 142,009 + -1.3 142,035 + +0.6 142,035 + +0.1 142,036 - -0.6 142,047 - -0.9 142,047 - -1.8 142,115 + +1.3 142,115 + -0.7 142,115 + +0.2 142,116 - +0.8 142,159 + +0.7 142,319 + GFF6080 0.24 +0.3 142,320 - GFF6080 0.24 -2.1
Or see this region's nucleotide sequence