Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5999 and GFF6000 are separated by 11 nucleotides GFF6000 and GFF6001 are separated by 10 nucleotides
GFF5999 - DNA polymerase IV (EC 2.7.7.7), at 57,501 to 58,688
GFF5999
GFF6000 - hypothetical protein, at 58,700 to 59,389
GFF6000
GFF6001 - Purine nucleoside permease, at 59,400 to 60,455
GFF6001
Position (kb) 58
59
60 Strain fitness (log2 ratio) -2
-1
0
1
2 at 57.702 kb on + strand, within GFF5999 at 57.801 kb on + strand, within GFF5999 at 57.882 kb on + strand, within GFF5999 at 57.882 kb on + strand, within GFF5999 at 57.882 kb on + strand, within GFF5999 at 57.882 kb on + strand, within GFF5999 at 57.882 kb on + strand, within GFF5999 at 57.883 kb on - strand, within GFF5999 at 57.883 kb on - strand, within GFF5999 at 57.883 kb on - strand, within GFF5999 at 57.883 kb on - strand, within GFF5999 at 57.883 kb on - strand, within GFF5999 at 57.883 kb on - strand, within GFF5999 at 58.049 kb on + strand, within GFF5999 at 58.049 kb on + strand, within GFF5999 at 58.050 kb on - strand, within GFF5999 at 58.416 kb on + strand, within GFF5999 at 58.416 kb on + strand, within GFF5999 at 58.417 kb on - strand, within GFF5999 at 58.417 kb on - strand, within GFF5999 at 58.417 kb on - strand, within GFF5999 at 58.417 kb on - strand, within GFF5999 at 58.417 kb on - strand, within GFF5999 at 58.417 kb on - strand, within GFF5999 at 58.417 kb on - strand, within GFF5999 at 58.450 kb on - strand, within GFF5999 at 58.450 kb on - strand, within GFF5999 at 58.450 kb on - strand, within GFF5999 at 58.767 kb on - strand at 58.767 kb on - strand at 58.767 kb on - strand at 58.767 kb on - strand at 58.767 kb on - strand at 58.858 kb on - strand, within GFF6000 at 58.883 kb on + strand, within GFF6000 at 58.883 kb on + strand, within GFF6000 at 58.884 kb on - strand, within GFF6000 at 58.884 kb on - strand, within GFF6000 at 58.884 kb on - strand, within GFF6000 at 58.884 kb on - strand, within GFF6000 at 58.927 kb on - strand, within GFF6000 at 58.927 kb on - strand, within GFF6000 at 58.927 kb on - strand, within GFF6000 at 59.004 kb on - strand, within GFF6000 at 59.093 kb on + strand, within GFF6000 at 59.094 kb on - strand, within GFF6000 at 59.094 kb on - strand, within GFF6000 at 59.094 kb on - strand, within GFF6000 at 59.094 kb on - strand, within GFF6000 at 59.153 kb on + strand, within GFF6000 at 59.247 kb on - strand, within GFF6000 at 59.247 kb on - strand, within GFF6000 at 59.247 kb on - strand, within GFF6000 at 59.247 kb on - strand, within GFF6000 at 59.247 kb on - strand, within GFF6000 at 59.264 kb on + strand, within GFF6000 at 59.264 kb on - strand, within GFF6000 at 59.296 kb on - strand, within GFF6000 at 59.371 kb on - strand at 59.371 kb on - strand at 59.371 kb on - strand at 59.371 kb on - strand at 59.371 kb on - strand at 59.371 kb on - strand at 59.668 kb on + strand, within GFF6001 at 59.668 kb on + strand, within GFF6001 at 59.668 kb on + strand, within GFF6001 at 59.669 kb on - strand, within GFF6001 at 59.683 kb on + strand, within GFF6001 at 59.684 kb on - strand, within GFF6001 at 59.698 kb on + strand, within GFF6001 at 59.698 kb on + strand, within GFF6001 at 59.698 kb on + strand, within GFF6001 at 59.698 kb on + strand, within GFF6001 at 59.698 kb on + strand, within GFF6001 at 59.698 kb on + strand, within GFF6001 at 59.699 kb on - strand, within GFF6001 at 59.699 kb on - strand, within GFF6001 at 59.699 kb on - strand, within GFF6001 at 59.699 kb on - strand, within GFF6001 at 59.750 kb on + strand, within GFF6001 at 59.751 kb on - strand, within GFF6001 at 59.751 kb on - strand, within GFF6001 at 59.751 kb on - strand, within GFF6001 at 59.751 kb on - strand, within GFF6001 at 59.915 kb on - strand, within GFF6001 at 59.987 kb on - strand, within GFF6001 at 59.987 kb on - strand, within GFF6001 at 60.067 kb on + strand, within GFF6001 at 60.067 kb on + strand, within GFF6001 at 60.067 kb on + strand, within GFF6001 at 60.068 kb on - strand, within GFF6001 at 60.068 kb on - strand, within GFF6001 at 60.068 kb on - strand, within GFF6001 at 60.068 kb on - strand, within GFF6001 at 60.070 kb on + strand, within GFF6001 at 60.070 kb on + strand, within GFF6001 at 60.070 kb on + strand, within GFF6001 at 60.070 kb on + strand, within GFF6001 at 60.070 kb on + strand, within GFF6001 at 60.071 kb on - strand, within GFF6001 at 60.071 kb on - strand, within GFF6001 at 60.071 kb on - strand, within GFF6001 at 60.071 kb on - strand, within GFF6001 at 60.071 kb on - strand, within GFF6001 at 60.181 kb on + strand, within GFF6001 at 60.182 kb on - strand, within GFF6001 at 60.182 kb on - strand, within GFF6001 at 60.182 kb on - strand, within GFF6001 at 60.182 kb on - strand, within GFF6001 at 60.233 kb on - strand, within GFF6001 at 60.242 kb on - strand, within GFF6001
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 57,702 + GFF5999 0.17 -0.2 57,801 + GFF5999 0.25 +0.0 57,882 + GFF5999 0.32 -0.7 57,882 + GFF5999 0.32 +0.2 57,882 + GFF5999 0.32 -0.7 57,882 + GFF5999 0.32 -0.4 57,882 + GFF5999 0.32 +0.3 57,883 - GFF5999 0.32 +0.1 57,883 - GFF5999 0.32 +0.8 57,883 - GFF5999 0.32 -0.1 57,883 - GFF5999 0.32 -0.6 57,883 - GFF5999 0.32 -2.1 57,883 - GFF5999 0.32 -0.0 58,049 + GFF5999 0.46 -1.4 58,049 + GFF5999 0.46 -0.1 58,050 - GFF5999 0.46 -0.6 58,416 + GFF5999 0.77 -1.2 58,416 + GFF5999 0.77 -0.5 58,417 - GFF5999 0.77 +0.1 58,417 - GFF5999 0.77 -0.3 58,417 - GFF5999 0.77 +0.1 58,417 - GFF5999 0.77 -0.3 58,417 - GFF5999 0.77 +0.1 58,417 - GFF5999 0.77 +0.9 58,417 - GFF5999 0.77 +0.6 58,450 - GFF5999 0.80 +0.1 58,450 - GFF5999 0.80 -1.2 58,450 - GFF5999 0.80 +1.6 58,767 - +0.7 58,767 - +0.3 58,767 - +0.7 58,767 - -1.2 58,767 - +0.2 58,858 - GFF6000 0.23 -0.2 58,883 + GFF6000 0.27 -0.1 58,883 + GFF6000 0.27 -0.7 58,884 - GFF6000 0.27 -0.6 58,884 - GFF6000 0.27 -0.4 58,884 - GFF6000 0.27 +0.9 58,884 - GFF6000 0.27 +0.6 58,927 - GFF6000 0.33 +0.0 58,927 - GFF6000 0.33 +0.4 58,927 - GFF6000 0.33 +0.1 59,004 - GFF6000 0.44 -0.1 59,093 + GFF6000 0.57 -0.4 59,094 - GFF6000 0.57 -0.8 59,094 - GFF6000 0.57 +0.5 59,094 - GFF6000 0.57 +0.4 59,094 - GFF6000 0.57 +0.4 59,153 + GFF6000 0.66 +0.2 59,247 - GFF6000 0.79 -0.1 59,247 - GFF6000 0.79 -0.4 59,247 - GFF6000 0.79 +0.3 59,247 - GFF6000 0.79 -0.1 59,247 - GFF6000 0.79 +0.1 59,264 + GFF6000 0.82 +0.9 59,264 - GFF6000 0.82 +2.3 59,296 - GFF6000 0.86 -0.5 59,371 - +0.2 59,371 - +0.3 59,371 - +0.6 59,371 - +0.9 59,371 - -1.2 59,371 - -1.1 59,668 + GFF6001 0.25 +0.4 59,668 + GFF6001 0.25 +0.3 59,668 + GFF6001 0.25 +0.5 59,669 - GFF6001 0.25 +0.9 59,683 + GFF6001 0.27 +2.6 59,684 - GFF6001 0.27 -0.4 59,698 + GFF6001 0.28 -0.4 59,698 + GFF6001 0.28 -1.1 59,698 + GFF6001 0.28 -1.7 59,698 + GFF6001 0.28 -0.7 59,698 + GFF6001 0.28 -0.2 59,698 + GFF6001 0.28 -0.3 59,699 - GFF6001 0.28 +0.2 59,699 - GFF6001 0.28 +0.3 59,699 - GFF6001 0.28 -0.1 59,699 - GFF6001 0.28 -0.3 59,750 + GFF6001 0.33 +0.3 59,751 - GFF6001 0.33 -0.4 59,751 - GFF6001 0.33 -1.8 59,751 - GFF6001 0.33 -0.0 59,751 - GFF6001 0.33 -1.2 59,915 - GFF6001 0.49 +0.2 59,987 - GFF6001 0.56 -0.2 59,987 - GFF6001 0.56 +0.3 60,067 + GFF6001 0.63 -0.0 60,067 + GFF6001 0.63 +0.2 60,067 + GFF6001 0.63 -0.0 60,068 - GFF6001 0.63 -0.6 60,068 - GFF6001 0.63 -0.8 60,068 - GFF6001 0.63 -1.1 60,068 - GFF6001 0.63 -0.3 60,070 + GFF6001 0.63 +0.2 60,070 + GFF6001 0.63 +0.1 60,070 + GFF6001 0.63 +0.4 60,070 + GFF6001 0.63 +1.0 60,070 + GFF6001 0.63 -0.5 60,071 - GFF6001 0.64 -0.6 60,071 - GFF6001 0.64 -0.4 60,071 - GFF6001 0.64 -0.2 60,071 - GFF6001 0.64 +1.3 60,071 - GFF6001 0.64 +0.9 60,181 + GFF6001 0.74 -0.4 60,182 - GFF6001 0.74 -0.1 60,182 - GFF6001 0.74 +0.0 60,182 - GFF6001 0.74 -0.4 60,182 - GFF6001 0.74 -0.3 60,233 - GFF6001 0.79 +0.6 60,242 - GFF6001 0.80 -2.1
Or see this region's nucleotide sequence