Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5994 and GFF5995 are separated by 121 nucleotides GFF5995 and GFF5996 overlap by 4 nucleotides
GFF5994 - Chlorogenate esterase, at 51,270 to 54,026
GFF5994
GFF5995 - LysR family transcriptional regulator STM3121, at 54,148 to 55,083
GFF5995
GFF5996 - BUG/TctC family periplasmic protein, at 55,080 to 56,075
GFF5996
Position (kb)
54
55
56 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 53.152 kb on + strand, within GFF5994 at 53.153 kb on - strand, within GFF5994 at 53.153 kb on - strand, within GFF5994 at 53.155 kb on + strand, within GFF5994 at 53.155 kb on + strand, within GFF5994 at 53.201 kb on - strand, within GFF5994 at 53.201 kb on - strand, within GFF5994 at 53.236 kb on + strand, within GFF5994 at 53.236 kb on + strand, within GFF5994 at 53.236 kb on + strand, within GFF5994 at 53.236 kb on + strand, within GFF5994 at 53.236 kb on + strand, within GFF5994 at 53.237 kb on - strand, within GFF5994 at 53.237 kb on - strand, within GFF5994 at 53.260 kb on + strand, within GFF5994 at 53.260 kb on + strand, within GFF5994 at 53.260 kb on + strand, within GFF5994 at 53.260 kb on + strand, within GFF5994 at 53.260 kb on + strand, within GFF5994 at 53.261 kb on - strand, within GFF5994 at 53.261 kb on - strand, within GFF5994 at 53.261 kb on - strand, within GFF5994 at 53.302 kb on + strand, within GFF5994 at 53.302 kb on + strand, within GFF5994 at 53.302 kb on + strand, within GFF5994 at 53.303 kb on - strand, within GFF5994 at 53.303 kb on - strand, within GFF5994 at 53.326 kb on + strand, within GFF5994 at 53.327 kb on - strand, within GFF5994 at 53.327 kb on - strand, within GFF5994 at 53.386 kb on + strand, within GFF5994 at 53.387 kb on - strand, within GFF5994 at 53.465 kb on - strand, within GFF5994 at 53.465 kb on - strand, within GFF5994 at 53.473 kb on + strand, within GFF5994 at 53.474 kb on - strand, within GFF5994 at 53.668 kb on + strand, within GFF5994 at 53.668 kb on + strand, within GFF5994 at 53.669 kb on - strand, within GFF5994 at 54.088 kb on + strand at 54.088 kb on + strand at 54.088 kb on + strand at 54.088 kb on + strand at 54.104 kb on + strand at 54.104 kb on + strand at 54.104 kb on + strand at 54.105 kb on - strand at 54.115 kb on - strand at 54.119 kb on + strand at 54.119 kb on + strand at 54.120 kb on - strand at 54.145 kb on + strand at 54.145 kb on + strand at 54.155 kb on + strand at 54.155 kb on + strand at 54.155 kb on + strand at 54.155 kb on + strand at 54.155 kb on + strand at 54.156 kb on - strand at 54.156 kb on - strand at 54.169 kb on + strand at 54.169 kb on + strand at 54.775 kb on + strand, within GFF5995 at 54.775 kb on + strand, within GFF5995 at 54.776 kb on - strand, within GFF5995 at 54.934 kb on + strand, within GFF5995 at 54.934 kb on + strand, within GFF5995 at 54.934 kb on + strand, within GFF5995 at 55.086 kb on - strand at 55.212 kb on + strand, within GFF5996 at 55.212 kb on + strand, within GFF5996 at 55.212 kb on + strand, within GFF5996 at 55.213 kb on - strand, within GFF5996 at 55.213 kb on - strand, within GFF5996 at 55.213 kb on - strand, within GFF5996 at 55.213 kb on - strand, within GFF5996 at 55.426 kb on - strand, within GFF5996 at 55.737 kb on + strand, within GFF5996 at 55.737 kb on + strand, within GFF5996 at 55.737 kb on + strand, within GFF5996 at 55.738 kb on - strand, within GFF5996 at 55.968 kb on + strand, within GFF5996 at 55.968 kb on + strand, within GFF5996 at 55.968 kb on + strand, within GFF5996 at 55.968 kb on + strand, within GFF5996 at 55.969 kb on - strand, within GFF5996 at 55.969 kb on - strand, within GFF5996 at 55.969 kb on - strand, within GFF5996
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 53,152 + GFF5994 0.68 -0.2 53,153 - GFF5994 0.68 -0.3 53,153 - GFF5994 0.68 -0.5 53,155 + GFF5994 0.68 -0.3 53,155 + GFF5994 0.68 +0.8 53,201 - GFF5994 0.70 +0.6 53,201 - GFF5994 0.70 +0.2 53,236 + GFF5994 0.71 -0.8 53,236 + GFF5994 0.71 -1.2 53,236 + GFF5994 0.71 +0.3 53,236 + GFF5994 0.71 -0.2 53,236 + GFF5994 0.71 -0.5 53,237 - GFF5994 0.71 +0.0 53,237 - GFF5994 0.71 -0.1 53,260 + GFF5994 0.72 -0.9 53,260 + GFF5994 0.72 -1.0 53,260 + GFF5994 0.72 -0.2 53,260 + GFF5994 0.72 +0.0 53,260 + GFF5994 0.72 -1.0 53,261 - GFF5994 0.72 +0.9 53,261 - GFF5994 0.72 -0.9 53,261 - GFF5994 0.72 -1.1 53,302 + GFF5994 0.74 -0.3 53,302 + GFF5994 0.74 -0.7 53,302 + GFF5994 0.74 +0.0 53,303 - GFF5994 0.74 -0.7 53,303 - GFF5994 0.74 -0.6 53,326 + GFF5994 0.75 -0.4 53,327 - GFF5994 0.75 -1.7 53,327 - GFF5994 0.75 -1.1 53,386 + GFF5994 0.77 -1.0 53,387 - GFF5994 0.77 +0.0 53,465 - GFF5994 0.80 +1.0 53,465 - GFF5994 0.80 -1.0 53,473 + GFF5994 0.80 +0.8 53,474 - GFF5994 0.80 -0.8 53,668 + GFF5994 0.87 +0.3 53,668 + GFF5994 0.87 -1.1 53,669 - GFF5994 0.87 +0.1 54,088 + -0.0 54,088 + -1.0 54,088 + -0.8 54,088 + +0.4 54,104 + +1.3 54,104 + -0.5 54,104 + +0.5 54,105 - -0.8 54,115 - -0.6 54,119 + -0.8 54,119 + -0.8 54,120 - -0.7 54,145 + +0.4 54,145 + +0.7 54,155 + +0.5 54,155 + +0.0 54,155 + -0.0 54,155 + +2.2 54,155 + -1.4 54,156 - +0.0 54,156 - -0.4 54,169 + -1.8 54,169 + -0.4 54,775 + GFF5995 0.67 -0.9 54,775 + GFF5995 0.67 -0.0 54,776 - GFF5995 0.67 -0.1 54,934 + GFF5995 0.84 +0.2 54,934 + GFF5995 0.84 +0.6 54,934 + GFF5995 0.84 +0.2 55,086 - +0.9 55,212 + GFF5996 0.13 -0.5 55,212 + GFF5996 0.13 -1.8 55,212 + GFF5996 0.13 -0.2 55,213 - GFF5996 0.13 +0.1 55,213 - GFF5996 0.13 -0.3 55,213 - GFF5996 0.13 -0.1 55,213 - GFF5996 0.13 -0.2 55,426 - GFF5996 0.35 -0.8 55,737 + GFF5996 0.66 +0.6 55,737 + GFF5996 0.66 +0.2 55,737 + GFF5996 0.66 -0.8 55,738 - GFF5996 0.66 -0.3 55,968 + GFF5996 0.89 -1.8 55,968 + GFF5996 0.89 +0.1 55,968 + GFF5996 0.89 -0.2 55,968 + GFF5996 0.89 -0.1 55,969 - GFF5996 0.89 -0.4 55,969 - GFF5996 0.89 -1.6 55,969 - GFF5996 0.89 -1.3
Or see this region's nucleotide sequence