Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5811 and GFF5812 are separated by 18 nucleotides GFF5812 and GFF5813 overlap by 4 nucleotides GFF5813 and GFF5814 are separated by 116 nucleotides
GFF5811 - Multidrug efflux system MdtABC-TolC, inner-membrane proton/drug antiporter MdtB-like, at 187,702 to 190,872
GFF5811
GFF5812 - DNA-binding heavy metal response regulator, at 190,891 to 191,631
GFF5812
GFF5813 - Heavy metal sensor histidine kinase, at 191,628 to 193,025
GFF5813
GFF5814 - Rhodanese-related sulfurtransferase, at 193,142 to 194,080
GFF5814
Position (kb) 191
192
193
194 Strain fitness (log2 ratio) -2
-1
0
1 at 190.763 kb on - strand at 190.763 kb on - strand at 190.763 kb on - strand at 190.763 kb on - strand at 190.768 kb on + strand at 190.768 kb on + strand at 190.769 kb on - strand at 190.769 kb on - strand at 190.792 kb on + strand at 190.966 kb on + strand, within GFF5812 at 191.573 kb on - strand at 191.748 kb on + strand at 192.637 kb on + strand, within GFF5813 at 192.637 kb on + strand, within GFF5813 at 192.638 kb on - strand, within GFF5813 at 192.977 kb on + strand at 192.977 kb on + strand at 193.712 kb on + strand, within GFF5814 at 193.943 kb on + strand, within GFF5814 at 193.943 kb on + strand, within GFF5814 at 193.944 kb on - strand, within GFF5814 at 193.944 kb on - strand, within GFF5814
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 190,763 - +0.3 190,763 - +0.4 190,763 - +1.1 190,763 - -0.4 190,768 + -1.1 190,768 + -1.6 190,769 - +1.3 190,769 - +0.7 190,792 + +0.2 190,966 + GFF5812 0.10 -0.2 191,573 - -2.0 191,748 + +0.1 192,637 + GFF5813 0.72 -0.8 192,637 + GFF5813 0.72 -0.0 192,638 - GFF5813 0.72 -0.8 192,977 + +0.2 192,977 + +0.2 193,712 + GFF5814 0.61 -1.1 193,943 + GFF5814 0.85 -0.4 193,943 + GFF5814 0.85 -0.8 193,944 - GFF5814 0.85 -0.7 193,944 - GFF5814 0.85 -0.3
Or see this region's nucleotide sequence