Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5740 and GFF5741 overlap by 4 nucleotides GFF5741 and GFF5742 overlap by 4 nucleotides GFF5742 and GFF5743 overlap by 8 nucleotides GFF5743 and GFF5744 are separated by 27 nucleotides GFF5744 and GFF5745 are separated by 154 nucleotides
GFF5740 - Flagellar biosynthesis protein FliP, at 114,036 to 114,794
GFF5740
GFF5741 - Flagellar biosynthesis protein FliO, at 114,791 to 115,294
GFF5741
GFF5742 - Flagellar motor switch protein FliN, at 115,291 to 115,761
GFF5742
GFF5743 - Flagellar motor switch protein FliM, at 115,754 to 116,758
GFF5743
GFF5744 - Flagellar basal body-associated protein FliL, at 116,786 to 117,289
GFF5744
GFF5745 - Flagellar hook-length control protein FliK, at 117,444 to 118,883
GFF5745
Position (kb)
115
116
117 Strain fitness (log2 ratio)
-2
-1
0
1 at 115.355 kb on + strand, within GFF5742 at 115.356 kb on - strand, within GFF5742 at 115.356 kb on - strand, within GFF5742 at 115.397 kb on + strand, within GFF5742 at 115.819 kb on - strand at 116.215 kb on - strand, within GFF5743 at 116.215 kb on - strand, within GFF5743 at 116.340 kb on + strand, within GFF5743 at 116.341 kb on - strand, within GFF5743 at 116.752 kb on - strand at 117.284 kb on - strand at 117.315 kb on + strand at 117.315 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 115,355 + GFF5742 0.14 +0.3 115,356 - GFF5742 0.14 -0.9 115,356 - GFF5742 0.14 -0.4 115,397 + GFF5742 0.23 -1.1 115,819 - -0.7 116,215 - GFF5743 0.46 -0.9 116,215 - GFF5743 0.46 -0.0 116,340 + GFF5743 0.58 -1.7 116,341 - GFF5743 0.58 -1.8 116,752 - +0.3 117,284 - +0.5 117,315 + -0.3 117,315 + +0.1
Or see this region's nucleotide sequence