Strain Fitness in Variovorax sp. SCN45 around GFF5690

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5688 and GFF5689 overlap by 25 nucleotidesGFF5689 and GFF5690 overlap by 4 nucleotidesGFF5690 and GFF5691 overlap by 4 nucleotidesGFF5691 and GFF5692 are separated by 157 nucleotides GFF5688 - Transcriptional regulator, LysR family, at 63,422 to 64,345 GFF5688 GFF5689 - Oxidoreductase, 2OG-Fe(II) oxygenase family, at 64,321 to 65,322 GFF5689 GFF5690 - Glutamate/aspartate ABC transporter, ATP-binding protein GltL (TC 3.A.1.3.4), at 65,319 to 66,062 GFF5690 GFF5691 - Glutamate/aspartate ABC transporter, permease protein GltK (TC 3.A.1.3.4), at 66,059 to 66,769 GFF5691 GFF5692 - Glutamate/aspartate ABC transporter, permease protein GltJ (TC 3.A.1.3.4), at 66,927 to 67,622 GFF5692 Position (kb) 65 66 67Strain fitness (log2 ratio) -2 -1 0 1 2 3at 64.376 kb on + strandat 64.376 kb on + strandat 64.376 kb on + strandat 64.376 kb on + strandat 64.376 kb on + strandat 64.377 kb on - strandat 64.377 kb on - strandat 64.377 kb on - strandat 64.572 kb on - strand, within GFF5689at 64.572 kb on - strand, within GFF5689at 64.572 kb on - strand, within GFF5689at 64.778 kb on + strand, within GFF5689at 64.779 kb on - strand, within GFF5689at 64.817 kb on + strand, within GFF5689at 64.818 kb on - strand, within GFF5689at 64.833 kb on - strand, within GFF5689at 64.907 kb on + strand, within GFF5689at 64.907 kb on + strand, within GFF5689at 64.907 kb on + strand, within GFF5689at 64.908 kb on - strand, within GFF5689at 64.908 kb on - strand, within GFF5689at 65.018 kb on + strand, within GFF5689at 65.018 kb on + strand, within GFF5689at 65.019 kb on - strand, within GFF5689at 65.019 kb on - strand, within GFF5689at 65.019 kb on - strand, within GFF5689at 65.207 kb on + strand, within GFF5689at 65.207 kb on + strand, within GFF5689at 65.207 kb on + strand, within GFF5689at 65.207 kb on + strand, within GFF5689at 65.208 kb on - strand, within GFF5689at 65.208 kb on - strand, within GFF5689at 65.208 kb on - strand, within GFF5689at 65.208 kb on - strand, within GFF5689at 65.208 kb on - strand, within GFF5689at 65.446 kb on - strand, within GFF5690at 65.446 kb on - strand, within GFF5690at 66.023 kb on - strandat 66.023 kb on - strandat 66.031 kb on + strandat 66.031 kb on + strandat 66.123 kb on + strandat 66.124 kb on - strandat 66.159 kb on + strand, within GFF5691at 66.159 kb on + strand, within GFF5691at 66.159 kb on + strand, within GFF5691at 66.159 kb on + strand, within GFF5691at 66.160 kb on - strand, within GFF5691at 66.160 kb on - strand, within GFF5691at 66.160 kb on - strand, within GFF5691at 66.173 kb on + strand, within GFF5691at 66.180 kb on + strand, within GFF5691at 66.180 kb on + strand, within GFF5691at 66.180 kb on + strand, within GFF5691at 66.180 kb on + strand, within GFF5691at 66.180 kb on + strand, within GFF5691at 66.181 kb on - strand, within GFF5691at 66.181 kb on - strand, within GFF5691at 66.181 kb on - strand, within GFF5691at 66.181 kb on - strand, within GFF5691at 66.181 kb on - strand, within GFF5691at 66.181 kb on - strand, within GFF5691at 66.258 kb on + strand, within GFF5691at 66.258 kb on + strand, within GFF5691at 66.258 kb on + strand, within GFF5691at 66.258 kb on + strand, within GFF5691at 66.258 kb on + strand, within GFF5691at 66.259 kb on - strand, within GFF5691at 66.259 kb on - strand, within GFF5691at 66.259 kb on - strand, within GFF5691at 66.259 kb on - strand, within GFF5691at 66.364 kb on - strand, within GFF5691at 66.528 kb on + strand, within GFF5691at 66.528 kb on + strand, within GFF5691at 66.529 kb on - strand, within GFF5691at 66.529 kb on - strand, within GFF5691at 66.529 kb on - strand, within GFF5691at 66.738 kb on + strandat 66.738 kb on + strandat 66.738 kb on + strandat 66.738 kb on + strandat 66.739 kb on - strandat 66.747 kb on + strandat 66.958 kb on + strandat 66.958 kb on + strandat 66.958 kb on + strandat 66.959 kb on - strandat 66.959 kb on - strandat 67.000 kb on + strand, within GFF5692at 67.000 kb on + strand, within GFF5692at 67.000 kb on + strand, within GFF5692at 67.001 kb on - strand, within GFF5692at 67.001 kb on - strand, within GFF5692at 67.001 kb on - strand, within GFF5692at 67.001 kb on - strand, within GFF5692

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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64,376 + -0.2
64,376 + -0.2
64,376 + -1.6
64,376 + +3.3
64,376 + +0.0
64,377 - +0.2
64,377 - +1.7
64,377 - +0.4
64,572 - GFF5689 0.25 +0.7
64,572 - GFF5689 0.25 -0.1
64,572 - GFF5689 0.25 -0.7
64,778 + GFF5689 0.46 +0.4
64,779 - GFF5689 0.46 -0.5
64,817 + GFF5689 0.50 -0.4
64,818 - GFF5689 0.50 -0.0
64,833 - GFF5689 0.51 -0.7
64,907 + GFF5689 0.58 -0.9
64,907 + GFF5689 0.58 +1.7
64,907 + GFF5689 0.58 -0.5
64,908 - GFF5689 0.59 -0.1
64,908 - GFF5689 0.59 +0.0
65,018 + GFF5689 0.70 -0.4
65,018 + GFF5689 0.70 -0.1
65,019 - GFF5689 0.70 -1.5
65,019 - GFF5689 0.70 -0.5
65,019 - GFF5689 0.70 -0.8
65,207 + GFF5689 0.88 -0.1
65,207 + GFF5689 0.88 -1.2
65,207 + GFF5689 0.88 +1.3
65,207 + GFF5689 0.88 -1.3
65,208 - GFF5689 0.89 -0.3
65,208 - GFF5689 0.89 -0.1
65,208 - GFF5689 0.89 -0.9
65,208 - GFF5689 0.89 +0.0
65,208 - GFF5689 0.89 -0.1
65,446 - GFF5690 0.17 -1.2
65,446 - GFF5690 0.17 -0.3
66,023 - -0.4
66,023 - -0.1
66,031 + +0.7
66,031 + -1.6
66,123 + -0.6
66,124 - -0.7
66,159 + GFF5691 0.14 -0.0
66,159 + GFF5691 0.14 -0.1
66,159 + GFF5691 0.14 +0.4
66,159 + GFF5691 0.14 -0.1
66,160 - GFF5691 0.14 -1.1
66,160 - GFF5691 0.14 -0.8
66,160 - GFF5691 0.14 -1.3
66,173 + GFF5691 0.16 -0.1
66,180 + GFF5691 0.17 -0.6
66,180 + GFF5691 0.17 -0.4
66,180 + GFF5691 0.17 -0.3
66,180 + GFF5691 0.17 -2.0
66,180 + GFF5691 0.17 -0.7
66,181 - GFF5691 0.17 +0.2
66,181 - GFF5691 0.17 +0.5
66,181 - GFF5691 0.17 -1.3
66,181 - GFF5691 0.17 +0.3
66,181 - GFF5691 0.17 +0.8
66,181 - GFF5691 0.17 +0.0
66,258 + GFF5691 0.28 +0.1
66,258 + GFF5691 0.28 -1.2
66,258 + GFF5691 0.28 -1.0
66,258 + GFF5691 0.28 +0.5
66,258 + GFF5691 0.28 -0.9
66,259 - GFF5691 0.28 -2.5
66,259 - GFF5691 0.28 -1.1
66,259 - GFF5691 0.28 +0.5
66,259 - GFF5691 0.28 +0.4
66,364 - GFF5691 0.43 +0.5
66,528 + GFF5691 0.66 -0.7
66,528 + GFF5691 0.66 -0.8
66,529 - GFF5691 0.66 -0.6
66,529 - GFF5691 0.66 +0.3
66,529 - GFF5691 0.66 -0.3
66,738 + +0.2
66,738 + +0.1
66,738 + +0.7
66,738 + -0.5
66,739 - +1.3
66,747 + -0.1
66,958 + +0.3
66,958 + -0.7
66,958 + -0.2
66,959 - +0.6
66,959 - +0.4
67,000 + GFF5692 0.10 -1.0
67,000 + GFF5692 0.10 -0.4
67,000 + GFF5692 0.10 -0.3
67,001 - GFF5692 0.11 -0.0
67,001 - GFF5692 0.11 -0.8
67,001 - GFF5692 0.11 +1.7
67,001 - GFF5692 0.11 -2.3

Or see this region's nucleotide sequence