Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5415 and GFF5416 are separated by 4 nucleotides GFF5416 and GFF5417 overlap by 4 nucleotides GFF5417 and GFF5418 are separated by 15 nucleotides
GFF5415 - Type IV fimbrial biogenesis protein PilV, at 39,046 to 39,537
GFF5415
GFF5416 - Type IV fimbrial biogenesis protein PilW, at 39,542 to 40,399
GFF5416
GFF5417 - hypothetical protein, at 40,396 to 40,965
GFF5417
GFF5418 - Type IV fimbrial biogenesis protein PilY1, at 40,981 to 45,978
GFF5418
Position (kb) 40
41 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 39.412 kb on - strand, within GFF5415 at 39.505 kb on + strand at 39.505 kb on + strand at 39.505 kb on + strand at 39.505 kb on + strand at 39.505 kb on + strand at 39.505 kb on + strand at 39.505 kb on + strand at 39.506 kb on - strand at 39.506 kb on - strand at 39.506 kb on - strand at 39.506 kb on - strand at 39.506 kb on - strand at 39.506 kb on - strand at 39.506 kb on - strand at 39.506 kb on - strand at 39.506 kb on - strand at 39.514 kb on + strand at 39.514 kb on + strand at 39.514 kb on + strand at 39.514 kb on + strand at 39.514 kb on + strand at 39.514 kb on + strand at 39.514 kb on + strand at 39.514 kb on + strand at 39.514 kb on + strand at 39.515 kb on - strand at 39.515 kb on - strand at 39.515 kb on - strand at 39.515 kb on - strand at 39.515 kb on - strand at 39.515 kb on - strand at 39.515 kb on - strand at 39.515 kb on - strand at 39.680 kb on + strand, within GFF5416 at 39.680 kb on + strand, within GFF5416 at 39.680 kb on + strand, within GFF5416 at 39.680 kb on + strand, within GFF5416 at 39.680 kb on + strand, within GFF5416 at 39.680 kb on + strand, within GFF5416 at 39.680 kb on + strand, within GFF5416 at 39.680 kb on + strand, within GFF5416 at 39.680 kb on + strand, within GFF5416 at 39.680 kb on + strand, within GFF5416 at 39.680 kb on + strand, within GFF5416 at 39.680 kb on + strand, within GFF5416 at 39.680 kb on + strand, within GFF5416 at 39.680 kb on + strand, within GFF5416 at 39.681 kb on - strand, within GFF5416 at 39.681 kb on - strand, within GFF5416 at 39.681 kb on - strand, within GFF5416 at 39.681 kb on - strand, within GFF5416 at 39.681 kb on - strand, within GFF5416 at 39.681 kb on - strand, within GFF5416 at 39.681 kb on - strand, within GFF5416 at 39.681 kb on - strand, within GFF5416 at 39.681 kb on - strand, within GFF5416 at 39.681 kb on - strand, within GFF5416 at 39.681 kb on - strand, within GFF5416 at 39.681 kb on - strand, within GFF5416 at 39.697 kb on + strand, within GFF5416 at 39.697 kb on + strand, within GFF5416 at 39.697 kb on + strand, within GFF5416 at 39.697 kb on + strand, within GFF5416 at 39.698 kb on - strand, within GFF5416 at 39.698 kb on - strand, within GFF5416 at 39.698 kb on - strand, within GFF5416 at 39.698 kb on - strand, within GFF5416 at 39.788 kb on + strand, within GFF5416 at 39.884 kb on + strand, within GFF5416 at 39.884 kb on + strand, within GFF5416 at 39.884 kb on + strand, within GFF5416 at 39.884 kb on + strand, within GFF5416 at 39.884 kb on + strand, within GFF5416 at 39.884 kb on + strand, within GFF5416 at 39.884 kb on + strand, within GFF5416 at 39.884 kb on + strand, within GFF5416 at 39.885 kb on - strand, within GFF5416 at 39.885 kb on - strand, within GFF5416 at 39.885 kb on - strand, within GFF5416 at 39.885 kb on - strand, within GFF5416 at 39.885 kb on - strand, within GFF5416 at 39.885 kb on - strand, within GFF5416 at 39.885 kb on - strand, within GFF5416 at 39.885 kb on - strand, within GFF5416 at 39.885 kb on - strand, within GFF5416 at 39.986 kb on + strand, within GFF5416 at 39.986 kb on + strand, within GFF5416 at 39.987 kb on - strand, within GFF5416 at 39.987 kb on - strand, within GFF5416 at 40.037 kb on + strand, within GFF5416 at 40.037 kb on + strand, within GFF5416 at 40.037 kb on + strand, within GFF5416 at 40.037 kb on + strand, within GFF5416 at 40.037 kb on + strand, within GFF5416 at 40.038 kb on - strand, within GFF5416 at 40.038 kb on - strand, within GFF5416 at 40.229 kb on + strand, within GFF5416 at 40.230 kb on - strand, within GFF5416 at 40.352 kb on + strand at 40.352 kb on + strand at 40.352 kb on + strand at 40.352 kb on + strand at 40.352 kb on + strand at 40.353 kb on - strand at 40.353 kb on - strand at 40.490 kb on + strand, within GFF5417 at 40.490 kb on + strand, within GFF5417 at 40.490 kb on + strand, within GFF5417 at 40.490 kb on + strand, within GFF5417 at 40.491 kb on - strand, within GFF5417 at 40.618 kb on + strand, within GFF5417 at 40.618 kb on + strand, within GFF5417 at 40.618 kb on + strand, within GFF5417 at 40.619 kb on - strand, within GFF5417 at 40.625 kb on + strand, within GFF5417 at 40.625 kb on + strand, within GFF5417 at 40.625 kb on + strand, within GFF5417 at 40.625 kb on + strand, within GFF5417 at 40.625 kb on + strand, within GFF5417 at 40.625 kb on + strand, within GFF5417 at 40.625 kb on + strand, within GFF5417 at 40.625 kb on + strand, within GFF5417 at 40.626 kb on - strand, within GFF5417 at 40.626 kb on - strand, within GFF5417 at 40.626 kb on - strand, within GFF5417 at 40.626 kb on - strand, within GFF5417 at 40.626 kb on - strand, within GFF5417 at 40.626 kb on - strand, within GFF5417 at 40.687 kb on + strand, within GFF5417 at 40.687 kb on + strand, within GFF5417 at 40.687 kb on + strand, within GFF5417 at 40.687 kb on + strand, within GFF5417 at 40.687 kb on + strand, within GFF5417 at 40.687 kb on + strand, within GFF5417 at 40.687 kb on + strand, within GFF5417 at 40.687 kb on + strand, within GFF5417 at 40.688 kb on - strand, within GFF5417 at 40.688 kb on - strand, within GFF5417 at 40.688 kb on - strand, within GFF5417 at 40.688 kb on - strand, within GFF5417 at 40.688 kb on - strand, within GFF5417 at 40.688 kb on - strand, within GFF5417 at 40.688 kb on - strand, within GFF5417 at 40.688 kb on - strand, within GFF5417 at 40.688 kb on - strand, within GFF5417 at 40.688 kb on - strand, within GFF5417 at 40.688 kb on - strand, within GFF5417 at 40.688 kb on - strand, within GFF5417 at 40.688 kb on - strand, within GFF5417 at 40.688 kb on - strand, within GFF5417 at 40.722 kb on + strand, within GFF5417 at 40.722 kb on + strand, within GFF5417 at 40.722 kb on + strand, within GFF5417 at 40.723 kb on - strand, within GFF5417 at 40.723 kb on - strand, within GFF5417 at 40.723 kb on - strand, within GFF5417 at 40.769 kb on - strand, within GFF5417 at 40.769 kb on - strand, within GFF5417 at 40.775 kb on - strand, within GFF5417 at 40.775 kb on - strand, within GFF5417 at 40.780 kb on + strand, within GFF5417 at 40.780 kb on + strand, within GFF5417 at 40.789 kb on + strand, within GFF5417 at 40.789 kb on + strand, within GFF5417 at 40.789 kb on + strand, within GFF5417 at 40.789 kb on + strand, within GFF5417 at 40.790 kb on - strand, within GFF5417 at 40.867 kb on + strand, within GFF5417 at 40.867 kb on + strand, within GFF5417 at 40.868 kb on - strand, within GFF5417 at 40.964 kb on - strand at 41.028 kb on + strand at 41.028 kb on + strand at 41.188 kb on + strand at 41.189 kb on - strand at 41.189 kb on - strand at 41.191 kb on + strand at 41.191 kb on + strand at 41.191 kb on + strand at 41.192 kb on - strand at 41.192 kb on - strand at 41.192 kb on - strand at 41.192 kb on - strand at 41.192 kb on - strand at 41.200 kb on + strand at 41.200 kb on + strand at 41.200 kb on + strand at 41.200 kb on + strand at 41.200 kb on + strand at 41.201 kb on - strand at 41.201 kb on - strand at 41.201 kb on - strand at 41.201 kb on - strand at 41.201 kb on - strand at 41.202 kb on + strand at 41.203 kb on - strand at 41.269 kb on + strand at 41.269 kb on + strand at 41.269 kb on + strand at 41.269 kb on + strand at 41.269 kb on + strand at 41.269 kb on + strand at 41.272 kb on + strand at 41.272 kb on + strand at 41.272 kb on + strand at 41.272 kb on + strand at 41.272 kb on + strand at 41.273 kb on - strand at 41.273 kb on - strand at 41.273 kb on - strand at 41.273 kb on - strand at 41.273 kb on - strand at 41.273 kb on - strand at 41.273 kb on - strand at 41.278 kb on + strand at 41.278 kb on + strand at 41.278 kb on + strand at 41.278 kb on + strand at 41.279 kb on - strand at 41.279 kb on - strand at 41.299 kb on + strand at 41.299 kb on + strand at 41.300 kb on - strand at 41.305 kb on + strand at 41.306 kb on - strand at 41.401 kb on + strand at 41.401 kb on + strand at 41.401 kb on + strand at 41.401 kb on + strand at 41.402 kb on - strand at 41.402 kb on - strand at 41.402 kb on - strand at 41.402 kb on - strand at 41.402 kb on - strand at 41.402 kb on - strand at 41.443 kb on + strand at 41.443 kb on + strand at 41.443 kb on + strand at 41.443 kb on + strand at 41.444 kb on - strand at 41.444 kb on - strand at 41.479 kb on - strand at 41.497 kb on + strand, within GFF5418 at 41.497 kb on + strand, within GFF5418 at 41.497 kb on + strand, within GFF5418 at 41.497 kb on + strand, within GFF5418 at 41.497 kb on + strand, within GFF5418 at 41.497 kb on + strand, within GFF5418 at 41.497 kb on + strand, within GFF5418 at 41.498 kb on - strand, within GFF5418 at 41.498 kb on - strand, within GFF5418 at 41.498 kb on - strand, within GFF5418 at 41.498 kb on - strand, within GFF5418 at 41.498 kb on - strand, within GFF5418 at 41.498 kb on - strand, within GFF5418 at 41.498 kb on - strand, within GFF5418 at 41.498 kb on - strand, within GFF5418 at 41.498 kb on - strand, within GFF5418 at 41.534 kb on - strand, within GFF5418 at 41.534 kb on - strand, within GFF5418 at 41.560 kb on + strand, within GFF5418 at 41.560 kb on + strand, within GFF5418 at 41.561 kb on - strand, within GFF5418 at 41.561 kb on - strand, within GFF5418 at 41.575 kb on + strand, within GFF5418 at 41.576 kb on - strand, within GFF5418 at 41.576 kb on - strand, within GFF5418 at 41.964 kb on + strand, within GFF5418 at 41.964 kb on + strand, within GFF5418 at 41.964 kb on + strand, within GFF5418 at 41.965 kb on - strand, within GFF5418 at 41.965 kb on - strand, within GFF5418 at 41.965 kb on - strand, within GFF5418 at 41.965 kb on - strand, within GFF5418
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 39,412 - GFF5415 0.74 -0.4 39,505 + +0.1 39,505 + -0.8 39,505 + -0.7 39,505 + -0.9 39,505 + -1.6 39,505 + -1.8 39,505 + -2.3 39,506 - -1.1 39,506 - -1.4 39,506 - -0.4 39,506 - -1.8 39,506 - -2.5 39,506 - -2.2 39,506 - +1.4 39,506 - -2.3 39,506 - -2.2 39,514 + -2.7 39,514 + -1.0 39,514 + -1.4 39,514 + +0.9 39,514 + -1.0 39,514 + -1.2 39,514 + -3.3 39,514 + -1.9 39,514 + -1.3 39,515 - -0.9 39,515 - -0.2 39,515 - -1.4 39,515 - -0.9 39,515 - -2.1 39,515 - -1.2 39,515 - -0.9 39,515 - -0.3 39,680 + GFF5416 0.16 -1.0 39,680 + GFF5416 0.16 -0.9 39,680 + GFF5416 0.16 -0.8 39,680 + GFF5416 0.16 -1.8 39,680 + GFF5416 0.16 +0.7 39,680 + GFF5416 0.16 -1.0 39,680 + GFF5416 0.16 +0.3 39,680 + GFF5416 0.16 -0.8 39,680 + GFF5416 0.16 -1.0 39,680 + GFF5416 0.16 -0.4 39,680 + GFF5416 0.16 -1.0 39,680 + GFF5416 0.16 -1.9 39,680 + GFF5416 0.16 -0.4 39,680 + GFF5416 0.16 -1.6 39,681 - GFF5416 0.16 -1.1 39,681 - GFF5416 0.16 -1.7 39,681 - GFF5416 0.16 -1.5 39,681 - GFF5416 0.16 +0.3 39,681 - GFF5416 0.16 -0.1 39,681 - GFF5416 0.16 -1.3 39,681 - GFF5416 0.16 -1.5 39,681 - GFF5416 0.16 -1.6 39,681 - GFF5416 0.16 -1.1 39,681 - GFF5416 0.16 -1.9 39,681 - GFF5416 0.16 -1.5 39,681 - GFF5416 0.16 -1.4 39,697 + GFF5416 0.18 -1.0 39,697 + GFF5416 0.18 -2.6 39,697 + GFF5416 0.18 -0.6 39,697 + GFF5416 0.18 -1.4 39,698 - GFF5416 0.18 -1.3 39,698 - GFF5416 0.18 -0.6 39,698 - GFF5416 0.18 +0.1 39,698 - GFF5416 0.18 -2.3 39,788 + GFF5416 0.29 -1.0 39,884 + GFF5416 0.40 -1.1 39,884 + GFF5416 0.40 -1.9 39,884 + GFF5416 0.40 -1.0 39,884 + GFF5416 0.40 -1.3 39,884 + GFF5416 0.40 -2.7 39,884 + GFF5416 0.40 -0.9 39,884 + GFF5416 0.40 -0.4 39,884 + GFF5416 0.40 +0.5 39,885 - GFF5416 0.40 -1.1 39,885 - GFF5416 0.40 -1.1 39,885 - GFF5416 0.40 -1.6 39,885 - GFF5416 0.40 -1.8 39,885 - GFF5416 0.40 -1.4 39,885 - GFF5416 0.40 -1.2 39,885 - GFF5416 0.40 -1.3 39,885 - GFF5416 0.40 -0.4 39,885 - GFF5416 0.40 -1.4 39,986 + GFF5416 0.52 -1.1 39,986 + GFF5416 0.52 -1.7 39,987 - GFF5416 0.52 -1.4 39,987 - GFF5416 0.52 +0.7 40,037 + GFF5416 0.58 -1.1 40,037 + GFF5416 0.58 -2.2 40,037 + GFF5416 0.58 -2.0 40,037 + GFF5416 0.58 -0.4 40,037 + GFF5416 0.58 -0.8 40,038 - GFF5416 0.58 -1.4 40,038 - GFF5416 0.58 -1.1 40,229 + GFF5416 0.80 -2.1 40,230 - GFF5416 0.80 -2.1 40,352 + -1.3 40,352 + -1.4 40,352 + -0.8 40,352 + -0.7 40,352 + -1.1 40,353 - -1.3 40,353 - -1.0 40,490 + GFF5417 0.16 -0.8 40,490 + GFF5417 0.16 +0.2 40,490 + GFF5417 0.16 -1.2 40,490 + GFF5417 0.16 -1.3 40,491 - GFF5417 0.17 -0.5 40,618 + GFF5417 0.39 +0.3 40,618 + GFF5417 0.39 -0.8 40,618 + GFF5417 0.39 +0.5 40,619 - GFF5417 0.39 -1.3 40,625 + GFF5417 0.40 -2.6 40,625 + GFF5417 0.40 -1.4 40,625 + GFF5417 0.40 -1.3 40,625 + GFF5417 0.40 -1.1 40,625 + GFF5417 0.40 -1.4 40,625 + GFF5417 0.40 -0.8 40,625 + GFF5417 0.40 -1.0 40,625 + GFF5417 0.40 -1.7 40,626 - GFF5417 0.40 -1.6 40,626 - GFF5417 0.40 -0.4 40,626 - GFF5417 0.40 -2.3 40,626 - GFF5417 0.40 +0.3 40,626 - GFF5417 0.40 -0.3 40,626 - GFF5417 0.40 -0.6 40,687 + GFF5417 0.51 -1.4 40,687 + GFF5417 0.51 -1.3 40,687 + GFF5417 0.51 -2.5 40,687 + GFF5417 0.51 -1.7 40,687 + GFF5417 0.51 -1.1 40,687 + GFF5417 0.51 -2.0 40,687 + GFF5417 0.51 -0.7 40,687 + GFF5417 0.51 -1.1 40,688 - GFF5417 0.51 -0.4 40,688 - GFF5417 0.51 -1.6 40,688 - GFF5417 0.51 -1.1 40,688 - GFF5417 0.51 -1.3 40,688 - GFF5417 0.51 -1.3 40,688 - GFF5417 0.51 +0.3 40,688 - GFF5417 0.51 +0.3 40,688 - GFF5417 0.51 -1.4 40,688 - GFF5417 0.51 -1.1 40,688 - GFF5417 0.51 -0.9 40,688 - GFF5417 0.51 -1.7 40,688 - GFF5417 0.51 -0.2 40,688 - GFF5417 0.51 -1.7 40,688 - GFF5417 0.51 -0.5 40,722 + GFF5417 0.57 -1.4 40,722 + GFF5417 0.57 -2.2 40,722 + GFF5417 0.57 -1.6 40,723 - GFF5417 0.57 +0.9 40,723 - GFF5417 0.57 -1.5 40,723 - GFF5417 0.57 -1.6 40,769 - GFF5417 0.65 -3.7 40,769 - GFF5417 0.65 -1.1 40,775 - GFF5417 0.66 -0.1 40,775 - GFF5417 0.66 -0.8 40,780 + GFF5417 0.67 -1.5 40,780 + GFF5417 0.67 -1.6 40,789 + GFF5417 0.69 -1.3 40,789 + GFF5417 0.69 -0.6 40,789 + GFF5417 0.69 +0.4 40,789 + GFF5417 0.69 -0.8 40,790 - GFF5417 0.69 -1.3 40,867 + GFF5417 0.83 -1.3 40,867 + GFF5417 0.83 -1.1 40,868 - GFF5417 0.83 -0.1 40,964 - +1.0 41,028 + -2.4 41,028 + -1.1 41,188 + -1.7 41,189 - -0.8 41,189 - -2.6 41,191 + -2.0 41,191 + -2.4 41,191 + -2.1 41,192 - -1.1 41,192 - -0.1 41,192 - -0.7 41,192 - -1.4 41,192 - -0.7 41,200 + -0.6 41,200 + -2.6 41,200 + -1.5 41,200 + -0.5 41,200 + -1.2 41,201 - +0.3 41,201 - -2.3 41,201 - +0.9 41,201 - -1.4 41,201 - -1.1 41,202 + -1.6 41,203 - -0.8 41,269 + -1.4 41,269 + -1.5 41,269 + -1.0 41,269 + -1.0 41,269 + -1.8 41,269 + -1.2 41,272 + -1.1 41,272 + -1.7 41,272 + -1.5 41,272 + -0.7 41,272 + -1.8 41,273 - -1.4 41,273 - -0.9 41,273 - -0.7 41,273 - +0.3 41,273 - -1.0 41,273 - -0.0 41,273 - -2.7 41,278 + -0.4 41,278 + -1.6 41,278 + -2.1 41,278 + -0.7 41,279 - -0.7 41,279 - -2.4 41,299 + -0.7 41,299 + -2.8 41,300 - -1.3 41,305 + -1.1 41,306 - -0.8 41,401 + -0.9 41,401 + -1.8 41,401 + -1.9 41,401 + -1.5 41,402 - +2.3 41,402 - -1.3 41,402 - -1.2 41,402 - -1.1 41,402 - -0.8 41,402 - +0.7 41,443 + -1.2 41,443 + -2.1 41,443 + -0.4 41,443 + -0.4 41,444 - -1.3 41,444 - -0.9 41,479 - -2.0 41,497 + GFF5418 0.10 -1.1 41,497 + GFF5418 0.10 -0.8 41,497 + GFF5418 0.10 -1.6 41,497 + GFF5418 0.10 -1.8 41,497 + GFF5418 0.10 -1.0 41,497 + GFF5418 0.10 -1.0 41,497 + GFF5418 0.10 -1.5 41,498 - GFF5418 0.10 -1.3 41,498 - GFF5418 0.10 +1.3 41,498 - GFF5418 0.10 -1.1 41,498 - GFF5418 0.10 -2.0 41,498 - GFF5418 0.10 -1.5 41,498 - GFF5418 0.10 +0.3 41,498 - GFF5418 0.10 -0.7 41,498 - GFF5418 0.10 -4.2 41,498 - GFF5418 0.10 -1.5 41,534 - GFF5418 0.11 -1.5 41,534 - GFF5418 0.11 -1.1 41,560 + GFF5418 0.12 -1.4 41,560 + GFF5418 0.12 -0.8 41,561 - GFF5418 0.12 -1.5 41,561 - GFF5418 0.12 -1.4 41,575 + GFF5418 0.12 -0.4 41,576 - GFF5418 0.12 -0.6 41,576 - GFF5418 0.12 -0.3 41,964 + GFF5418 0.20 -0.1 41,964 + GFF5418 0.20 -1.2 41,964 + GFF5418 0.20 -1.8 41,965 - GFF5418 0.20 -1.2 41,965 - GFF5418 0.20 -1.7 41,965 - GFF5418 0.20 -2.3 41,965 - GFF5418 0.20 -2.2
Or see this region's nucleotide sequence