Strain Fitness in Variovorax sp. SCN45 around GFF5303

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5302 and GFF5303 are separated by 174 nucleotidesGFF5303 and GFF5304 are separated by 1 nucleotides GFF5302 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 177,712 to 178,752 GFF5302 GFF5303 - Tricarboxylate transport transcriptional regulator TctD, at 178,927 to 179,601 GFF5303 GFF5304 - no description, at 179,603 to 181,039 GFF5304 Position (kb) 178 179 180Strain fitness (log2 ratio) -2 -1 0 1 2at 177.971 kb on + strand, within GFF5302at 177.971 kb on + strand, within GFF5302at 177.971 kb on + strand, within GFF5302at 177.971 kb on + strand, within GFF5302at 177.972 kb on - strand, within GFF5302at 177.972 kb on - strand, within GFF5302at 177.972 kb on - strand, within GFF5302at 177.972 kb on - strand, within GFF5302at 177.972 kb on - strand, within GFF5302at 178.049 kb on + strand, within GFF5302at 178.049 kb on + strand, within GFF5302at 178.049 kb on + strand, within GFF5302at 178.050 kb on - strand, within GFF5302at 178.050 kb on - strand, within GFF5302at 178.067 kb on + strand, within GFF5302at 178.067 kb on + strand, within GFF5302at 178.245 kb on - strand, within GFF5302at 178.280 kb on + strand, within GFF5302at 178.475 kb on + strand, within GFF5302at 178.475 kb on + strand, within GFF5302at 178.475 kb on + strand, within GFF5302at 178.475 kb on + strand, within GFF5302at 178.476 kb on - strand, within GFF5302at 178.476 kb on - strand, within GFF5302at 178.476 kb on - strand, within GFF5302at 178.580 kb on + strand, within GFF5302at 178.580 kb on + strand, within GFF5302at 178.580 kb on + strand, within GFF5302at 178.581 kb on - strand, within GFF5302at 178.613 kb on + strand, within GFF5302at 178.614 kb on - strand, within GFF5302at 178.770 kb on + strandat 178.770 kb on + strandat 178.770 kb on + strandat 178.770 kb on + strandat 178.771 kb on - strandat 178.771 kb on - strandat 178.817 kb on + strandat 178.818 kb on - strandat 178.871 kb on + strandat 178.897 kb on + strandat 178.897 kb on + strandat 178.897 kb on + strandat 178.898 kb on - strandat 179.059 kb on + strand, within GFF5303at 179.059 kb on + strand, within GFF5303at 179.060 kb on - strand, within GFF5303at 179.219 kb on - strand, within GFF5303at 179.219 kb on - strand, within GFF5303at 179.219 kb on - strand, within GFF5303at 179.326 kb on + strand, within GFF5303at 179.326 kb on + strand, within GFF5303at 179.327 kb on - strand, within GFF5303at 179.459 kb on + strand, within GFF5303at 179.459 kb on + strand, within GFF5303at 179.503 kb on + strand, within GFF5303at 179.503 kb on + strand, within GFF5303at 179.503 kb on + strand, within GFF5303at 179.503 kb on + strand, within GFF5303at 179.503 kb on + strand, within GFF5303at 179.503 kb on + strand, within GFF5303at 179.503 kb on + strand, within GFF5303at 179.503 kb on + strand, within GFF5303at 179.503 kb on + strand, within GFF5303at 179.503 kb on + strand, within GFF5303at 179.503 kb on + strand, within GFF5303at 179.503 kb on + strand, within GFF5303at 179.503 kb on + strand, within GFF5303at 179.503 kb on + strand, within GFF5303at 179.503 kb on + strand, within GFF5303at 179.504 kb on - strand, within GFF5303at 179.504 kb on - strand, within GFF5303at 179.504 kb on - strand, within GFF5303at 179.504 kb on - strand, within GFF5303at 179.504 kb on - strand, within GFF5303at 179.504 kb on - strand, within GFF5303at 179.504 kb on - strand, within GFF5303at 179.504 kb on - strand, within GFF5303at 179.504 kb on - strand, within GFF5303at 179.504 kb on - strand, within GFF5303at 179.504 kb on - strand, within GFF5303at 179.573 kb on - strandat 179.599 kb on + strandat 179.599 kb on + strandat 179.600 kb on - strandat 179.721 kb on - strandat 179.721 kb on - strandat 179.958 kb on - strand, within GFF5304at 179.958 kb on - strand, within GFF5304at 179.958 kb on - strand, within GFF5304at 180.014 kb on + strand, within GFF5304at 180.014 kb on + strand, within GFF5304at 180.113 kb on + strand, within GFF5304at 180.113 kb on + strand, within GFF5304at 180.113 kb on + strand, within GFF5304at 180.113 kb on + strand, within GFF5304at 180.114 kb on - strand, within GFF5304at 180.114 kb on - strand, within GFF5304at 180.410 kb on + strand, within GFF5304

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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177,971 + GFF5302 0.25 +0.7
177,971 + GFF5302 0.25 +0.2
177,971 + GFF5302 0.25 +0.3
177,971 + GFF5302 0.25 +0.0
177,972 - GFF5302 0.25 -0.2
177,972 - GFF5302 0.25 -0.9
177,972 - GFF5302 0.25 -0.5
177,972 - GFF5302 0.25 -0.1
177,972 - GFF5302 0.25 -0.9
178,049 + GFF5302 0.32 +0.5
178,049 + GFF5302 0.32 +0.1
178,049 + GFF5302 0.32 +1.0
178,050 - GFF5302 0.32 -0.5
178,050 - GFF5302 0.32 -0.7
178,067 + GFF5302 0.34 +1.0
178,067 + GFF5302 0.34 +1.0
178,245 - GFF5302 0.51 -1.1
178,280 + GFF5302 0.55 +2.7
178,475 + GFF5302 0.73 -0.7
178,475 + GFF5302 0.73 -1.1
178,475 + GFF5302 0.73 -1.0
178,475 + GFF5302 0.73 -1.5
178,476 - GFF5302 0.73 -0.2
178,476 - GFF5302 0.73 +0.2
178,476 - GFF5302 0.73 -1.5
178,580 + GFF5302 0.83 -0.5
178,580 + GFF5302 0.83 -0.6
178,580 + GFF5302 0.83 +0.1
178,581 - GFF5302 0.83 -0.1
178,613 + GFF5302 0.87 +0.3
178,614 - GFF5302 0.87 -0.1
178,770 + +0.1
178,770 + -0.1
178,770 + -1.5
178,770 + -0.5
178,771 - +1.7
178,771 - +0.9
178,817 + +0.1
178,818 - +1.4
178,871 + -0.3
178,897 + -1.3
178,897 + -0.7
178,897 + -0.6
178,898 - -1.0
179,059 + GFF5303 0.20 -0.8
179,059 + GFF5303 0.20 +0.4
179,060 - GFF5303 0.20 +0.6
179,219 - GFF5303 0.43 -0.1
179,219 - GFF5303 0.43 -0.5
179,219 - GFF5303 0.43 +0.1
179,326 + GFF5303 0.59 -1.9
179,326 + GFF5303 0.59 -0.1
179,327 - GFF5303 0.59 -0.7
179,459 + GFF5303 0.79 +0.3
179,459 + GFF5303 0.79 +0.7
179,503 + GFF5303 0.85 +1.0
179,503 + GFF5303 0.85 +0.9
179,503 + GFF5303 0.85 +0.5
179,503 + GFF5303 0.85 -0.1
179,503 + GFF5303 0.85 -1.2
179,503 + GFF5303 0.85 +0.5
179,503 + GFF5303 0.85 -0.1
179,503 + GFF5303 0.85 +0.1
179,503 + GFF5303 0.85 +0.1
179,503 + GFF5303 0.85 -0.5
179,503 + GFF5303 0.85 +1.4
179,503 + GFF5303 0.85 +0.3
179,503 + GFF5303 0.85 +0.1
179,503 + GFF5303 0.85 +1.7
179,503 + GFF5303 0.85 -0.1
179,504 - GFF5303 0.85 -0.3
179,504 - GFF5303 0.85 -0.7
179,504 - GFF5303 0.85 -0.9
179,504 - GFF5303 0.85 -0.7
179,504 - GFF5303 0.85 -0.7
179,504 - GFF5303 0.85 -0.7
179,504 - GFF5303 0.85 +0.1
179,504 - GFF5303 0.85 +0.7
179,504 - GFF5303 0.85 -0.7
179,504 - GFF5303 0.85 +0.1
179,504 - GFF5303 0.85 -0.4
179,573 - -0.1
179,599 + -0.3
179,599 + +0.9
179,600 - -0.2
179,721 - -1.5
179,721 - -0.7
179,958 - GFF5304 0.25 +0.1
179,958 - GFF5304 0.25 +1.1
179,958 - GFF5304 0.25 +0.5
180,014 + GFF5304 0.29 -1.1
180,014 + GFF5304 0.29 -0.2
180,113 + GFF5304 0.35 -0.5
180,113 + GFF5304 0.35 +0.1
180,113 + GFF5304 0.35 -1.1
180,113 + GFF5304 0.35 +0.2
180,114 - GFF5304 0.36 -0.2
180,114 - GFF5304 0.36 +0.2
180,410 + GFF5304 0.56 +0.8

Or see this region's nucleotide sequence