Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5224 and GFF5225 are separated by 106 nucleotides GFF5225 and GFF5226 overlap by 4 nucleotides GFF5226 and GFF5227 are separated by 14 nucleotides
GFF5224 - Uncharacterized MFS-type transporter, at 87,257 to 88,528
GFF5224
GFF5225 - hypothetical protein, at 88,635 to 88,823
GFF5225
GFF5226 - Probable cytochrome c2, at 88,820 to 89,536
GFF5226
GFF5227 - Putative diheme cytochrome c-553, at 89,551 to 90,837
GFF5227
Position (kb) 88
89
90 Strain fitness (log2 ratio) -1
0
1
2 at 88.022 kb on + strand, within GFF5224 at 88.022 kb on + strand, within GFF5224 at 88.022 kb on + strand, within GFF5224 at 88.220 kb on + strand, within GFF5224 at 88.221 kb on - strand, within GFF5224 at 88.271 kb on + strand, within GFF5224 at 88.386 kb on + strand, within GFF5224 at 88.406 kb on + strand at 88.406 kb on + strand at 88.566 kb on - strand at 88.566 kb on - strand at 88.566 kb on - strand at 89.021 kb on + strand, within GFF5226 at 89.081 kb on + strand, within GFF5226 at 89.081 kb on + strand, within GFF5226 at 89.081 kb on + strand, within GFF5226 at 89.082 kb on - strand, within GFF5226 at 89.082 kb on - strand, within GFF5226 at 89.082 kb on - strand, within GFF5226 at 89.082 kb on - strand, within GFF5226 at 89.082 kb on - strand, within GFF5226 at 89.130 kb on - strand, within GFF5226 at 89.153 kb on + strand, within GFF5226 at 89.153 kb on + strand, within GFF5226 at 89.327 kb on + strand, within GFF5226 at 89.328 kb on - strand, within GFF5226 at 89.328 kb on - strand, within GFF5226 at 89.714 kb on - strand, within GFF5227 at 89.714 kb on - strand, within GFF5227 at 89.771 kb on - strand, within GFF5227 at 89.812 kb on + strand, within GFF5227 at 89.812 kb on + strand, within GFF5227 at 89.812 kb on + strand, within GFF5227 at 89.812 kb on + strand, within GFF5227 at 89.813 kb on - strand, within GFF5227 at 89.980 kb on + strand, within GFF5227 at 89.981 kb on - strand, within GFF5227 at 89.981 kb on - strand, within GFF5227 at 89.981 kb on - strand, within GFF5227 at 89.986 kb on + strand, within GFF5227 at 89.986 kb on + strand, within GFF5227 at 89.987 kb on - strand, within GFF5227 at 90.148 kb on + strand, within GFF5227 at 90.148 kb on + strand, within GFF5227 at 90.262 kb on + strand, within GFF5227 at 90.263 kb on - strand, within GFF5227 at 90.407 kb on - strand, within GFF5227 at 90.442 kb on + strand, within GFF5227
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 88,022 + GFF5224 0.60 +0.9 88,022 + GFF5224 0.60 +0.7 88,022 + GFF5224 0.60 -0.4 88,220 + GFF5224 0.76 -0.8 88,221 - GFF5224 0.76 +0.4 88,271 + GFF5224 0.80 -0.3 88,386 + GFF5224 0.89 -0.2 88,406 + +0.1 88,406 + -0.8 88,566 - -0.5 88,566 - +0.7 88,566 - +0.4 89,021 + GFF5226 0.28 +1.2 89,081 + GFF5226 0.36 -0.8 89,081 + GFF5226 0.36 +0.0 89,081 + GFF5226 0.36 -0.1 89,082 - GFF5226 0.37 -0.2 89,082 - GFF5226 0.37 -0.2 89,082 - GFF5226 0.37 +0.7 89,082 - GFF5226 0.37 +1.7 89,082 - GFF5226 0.37 +0.0 89,130 - GFF5226 0.43 -0.3 89,153 + GFF5226 0.46 +0.6 89,153 + GFF5226 0.46 -0.3 89,327 + GFF5226 0.71 +0.1 89,328 - GFF5226 0.71 -0.5 89,328 - GFF5226 0.71 +0.3 89,714 - GFF5227 0.13 +0.9 89,714 - GFF5227 0.13 -0.7 89,771 - GFF5227 0.17 -0.3 89,812 + GFF5227 0.20 -1.0 89,812 + GFF5227 0.20 -0.5 89,812 + GFF5227 0.20 +1.7 89,812 + GFF5227 0.20 +0.5 89,813 - GFF5227 0.20 -0.5 89,980 + GFF5227 0.33 -0.1 89,981 - GFF5227 0.33 +0.1 89,981 - GFF5227 0.33 -0.6 89,981 - GFF5227 0.33 -0.4 89,986 + GFF5227 0.34 -0.1 89,986 + GFF5227 0.34 +0.7 89,987 - GFF5227 0.34 -1.2 90,148 + GFF5227 0.46 -0.9 90,148 + GFF5227 0.46 -0.5 90,262 + GFF5227 0.55 +0.3 90,263 - GFF5227 0.55 -0.3 90,407 - GFF5227 0.67 +2.2 90,442 + GFF5227 0.69 -0.2
Or see this region's nucleotide sequence