Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5163 and GFF5164 are separated by 9 nucleotides GFF5164 and GFF5165 are separated by 9 nucleotides
GFF5163 - Soluble lytic murein transglycosylase (EC 4.2.2.n1), at 15,895 to 17,925
GFF5163
GFF5164 - 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2), at 17,935 to 18,552
GFF5164
GFF5165 - Transcriptional regulator, LysR family, at 18,562 to 19,545
GFF5165
Position (kb)
17
18
19 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 16.994 kb on + strand, within GFF5163 at 16.994 kb on + strand, within GFF5163 at 16.994 kb on + strand, within GFF5163 at 16.995 kb on - strand, within GFF5163 at 16.995 kb on - strand, within GFF5163 at 17.483 kb on + strand, within GFF5163 at 17.483 kb on + strand, within GFF5163 at 17.483 kb on + strand, within GFF5163 at 17.483 kb on + strand, within GFF5163 at 17.484 kb on - strand, within GFF5163 at 17.484 kb on - strand, within GFF5163 at 17.484 kb on - strand, within GFF5163 at 17.484 kb on - strand, within GFF5163 at 17.484 kb on - strand, within GFF5163 at 17.491 kb on + strand, within GFF5163 at 17.491 kb on + strand, within GFF5163 at 17.491 kb on + strand, within GFF5163 at 17.491 kb on + strand, within GFF5163 at 17.491 kb on + strand, within GFF5163 at 17.491 kb on + strand, within GFF5163 at 17.491 kb on + strand, within GFF5163 at 17.491 kb on + strand, within GFF5163 at 17.491 kb on + strand, within GFF5163 at 17.491 kb on + strand, within GFF5163 at 17.491 kb on + strand, within GFF5163 at 17.492 kb on - strand, within GFF5163 at 17.492 kb on - strand, within GFF5163 at 17.492 kb on - strand, within GFF5163 at 17.492 kb on - strand, within GFF5163 at 17.492 kb on - strand, within GFF5163 at 17.603 kb on + strand, within GFF5163 at 17.603 kb on + strand, within GFF5163 at 17.603 kb on + strand, within GFF5163 at 17.603 kb on + strand, within GFF5163 at 17.603 kb on + strand, within GFF5163 at 17.604 kb on - strand, within GFF5163 at 17.604 kb on - strand, within GFF5163 at 17.604 kb on - strand, within GFF5163 at 17.604 kb on - strand, within GFF5163 at 17.615 kb on + strand, within GFF5163 at 17.615 kb on + strand, within GFF5163 at 17.616 kb on - strand, within GFF5163 at 17.616 kb on - strand, within GFF5163 at 17.616 kb on - strand, within GFF5163 at 17.681 kb on + strand, within GFF5163 at 17.681 kb on + strand, within GFF5163 at 17.681 kb on + strand, within GFF5163 at 17.682 kb on - strand, within GFF5163 at 17.934 kb on + strand at 17.934 kb on + strand at 17.934 kb on + strand at 17.935 kb on - strand at 17.935 kb on - strand at 17.935 kb on - strand at 17.935 kb on - strand at 18.112 kb on + strand, within GFF5164 at 18.112 kb on + strand, within GFF5164 at 18.113 kb on - strand, within GFF5164 at 18.113 kb on - strand, within GFF5164 at 18.113 kb on - strand, within GFF5164 at 18.113 kb on - strand, within GFF5164 at 18.259 kb on + strand, within GFF5164 at 18.290 kb on - strand, within GFF5164 at 18.290 kb on - strand, within GFF5164 at 18.358 kb on + strand, within GFF5164 at 18.358 kb on + strand, within GFF5164 at 18.359 kb on - strand, within GFF5164 at 18.359 kb on - strand, within GFF5164 at 18.359 kb on - strand, within GFF5164 at 18.359 kb on - strand, within GFF5164 at 18.359 kb on - strand, within GFF5164 at 18.400 kb on + strand, within GFF5164 at 18.400 kb on + strand, within GFF5164 at 18.401 kb on - strand, within GFF5164 at 18.401 kb on - strand, within GFF5164 at 18.401 kb on - strand, within GFF5164 at 18.401 kb on - strand, within GFF5164 at 18.401 kb on - strand, within GFF5164 at 18.701 kb on + strand, within GFF5165 at 18.701 kb on + strand, within GFF5165 at 18.702 kb on - strand, within GFF5165 at 18.702 kb on - strand, within GFF5165 at 18.702 kb on - strand, within GFF5165 at 18.833 kb on + strand, within GFF5165 at 18.833 kb on + strand, within GFF5165 at 18.834 kb on - strand, within GFF5165 at 18.839 kb on + strand, within GFF5165 at 18.839 kb on + strand, within GFF5165 at 18.839 kb on + strand, within GFF5165 at 18.839 kb on + strand, within GFF5165 at 18.839 kb on + strand, within GFF5165 at 18.839 kb on + strand, within GFF5165 at 18.839 kb on + strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.840 kb on - strand, within GFF5165 at 18.848 kb on + strand, within GFF5165 at 18.848 kb on + strand, within GFF5165 at 18.848 kb on + strand, within GFF5165 at 18.849 kb on - strand, within GFF5165 at 18.849 kb on - strand, within GFF5165 at 18.849 kb on - strand, within GFF5165 at 18.849 kb on - strand, within GFF5165 at 19.157 kb on - strand, within GFF5165 at 19.264 kb on + strand, within GFF5165 at 19.265 kb on - strand, within GFF5165 at 19.265 kb on - strand, within GFF5165 at 19.265 kb on - strand, within GFF5165 at 19.265 kb on - strand, within GFF5165 at 19.265 kb on - strand, within GFF5165 at 19.265 kb on - strand, within GFF5165
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 16,994 + GFF5163 0.54 -0.3 16,994 + GFF5163 0.54 -0.4 16,994 + GFF5163 0.54 +0.1 16,995 - GFF5163 0.54 +0.6 16,995 - GFF5163 0.54 +0.0 17,483 + GFF5163 0.78 -0.3 17,483 + GFF5163 0.78 -0.1 17,483 + GFF5163 0.78 +0.2 17,483 + GFF5163 0.78 -0.2 17,484 - GFF5163 0.78 +0.2 17,484 - GFF5163 0.78 +0.1 17,484 - GFF5163 0.78 -0.9 17,484 - GFF5163 0.78 +1.7 17,484 - GFF5163 0.78 -0.4 17,491 + GFF5163 0.79 -0.2 17,491 + GFF5163 0.79 +0.5 17,491 + GFF5163 0.79 -0.1 17,491 + GFF5163 0.79 +0.4 17,491 + GFF5163 0.79 -0.3 17,491 + GFF5163 0.79 -0.3 17,491 + GFF5163 0.79 +0.3 17,491 + GFF5163 0.79 -0.5 17,491 + GFF5163 0.79 -0.6 17,491 + GFF5163 0.79 -1.3 17,491 + GFF5163 0.79 +0.6 17,492 - GFF5163 0.79 +0.0 17,492 - GFF5163 0.79 +0.4 17,492 - GFF5163 0.79 -0.4 17,492 - GFF5163 0.79 +0.1 17,492 - GFF5163 0.79 -0.2 17,603 + GFF5163 0.84 -0.3 17,603 + GFF5163 0.84 -0.6 17,603 + GFF5163 0.84 -0.3 17,603 + GFF5163 0.84 -0.1 17,603 + GFF5163 0.84 -0.8 17,604 - GFF5163 0.84 -1.6 17,604 - GFF5163 0.84 -1.5 17,604 - GFF5163 0.84 +0.5 17,604 - GFF5163 0.84 -0.1 17,615 + GFF5163 0.85 -0.2 17,615 + GFF5163 0.85 +0.2 17,616 - GFF5163 0.85 +2.2 17,616 - GFF5163 0.85 -1.5 17,616 - GFF5163 0.85 +0.1 17,681 + GFF5163 0.88 -0.2 17,681 + GFF5163 0.88 -1.1 17,681 + GFF5163 0.88 -0.3 17,682 - GFF5163 0.88 +0.7 17,934 + -0.1 17,934 + +0.0 17,934 + -0.5 17,935 - +0.2 17,935 - -0.7 17,935 - -0.4 17,935 - +1.7 18,112 + GFF5164 0.29 -0.9 18,112 + GFF5164 0.29 -0.6 18,113 - GFF5164 0.29 -0.7 18,113 - GFF5164 0.29 +0.3 18,113 - GFF5164 0.29 +0.3 18,113 - GFF5164 0.29 -2.6 18,259 + GFF5164 0.52 -0.5 18,290 - GFF5164 0.57 -1.3 18,290 - GFF5164 0.57 -1.1 18,358 + GFF5164 0.68 -0.8 18,358 + GFF5164 0.68 -1.2 18,359 - GFF5164 0.69 +0.2 18,359 - GFF5164 0.69 -0.9 18,359 - GFF5164 0.69 -0.3 18,359 - GFF5164 0.69 -0.8 18,359 - GFF5164 0.69 +0.3 18,400 + GFF5164 0.75 +0.1 18,400 + GFF5164 0.75 -1.1 18,401 - GFF5164 0.75 +0.3 18,401 - GFF5164 0.75 -0.5 18,401 - GFF5164 0.75 -2.5 18,401 - GFF5164 0.75 -0.1 18,401 - GFF5164 0.75 -0.7 18,701 + GFF5165 0.14 -0.3 18,701 + GFF5165 0.14 -0.4 18,702 - GFF5165 0.14 -0.8 18,702 - GFF5165 0.14 -0.6 18,702 - GFF5165 0.14 -1.2 18,833 + GFF5165 0.28 +0.0 18,833 + GFF5165 0.28 -0.7 18,834 - GFF5165 0.28 -1.9 18,839 + GFF5165 0.28 +0.7 18,839 + GFF5165 0.28 +0.3 18,839 + GFF5165 0.28 +0.9 18,839 + GFF5165 0.28 -0.1 18,839 + GFF5165 0.28 -0.7 18,839 + GFF5165 0.28 -0.6 18,839 + GFF5165 0.28 +0.4 18,840 - GFF5165 0.28 -0.2 18,840 - GFF5165 0.28 +1.1 18,840 - GFF5165 0.28 -1.0 18,840 - GFF5165 0.28 -0.0 18,840 - GFF5165 0.28 -0.3 18,840 - GFF5165 0.28 -0.2 18,840 - GFF5165 0.28 +1.7 18,840 - GFF5165 0.28 +2.5 18,840 - GFF5165 0.28 -0.7 18,840 - GFF5165 0.28 -0.5 18,840 - GFF5165 0.28 +0.1 18,840 - GFF5165 0.28 -0.1 18,848 + GFF5165 0.29 -0.0 18,848 + GFF5165 0.29 +0.0 18,848 + GFF5165 0.29 +0.7 18,849 - GFF5165 0.29 +0.4 18,849 - GFF5165 0.29 +0.3 18,849 - GFF5165 0.29 -0.1 18,849 - GFF5165 0.29 -2.0 19,157 - GFF5165 0.60 -0.7 19,264 + GFF5165 0.71 +0.1 19,265 - GFF5165 0.71 -0.3 19,265 - GFF5165 0.71 +0.1 19,265 - GFF5165 0.71 +0.7 19,265 - GFF5165 0.71 -0.1 19,265 - GFF5165 0.71 +0.4 19,265 - GFF5165 0.71 +0.3
Or see this region's nucleotide sequence