Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5098 and GFF5099 are separated by 11 nucleotides GFF5099 and GFF5100 are separated by 29 nucleotides GFF5100 and GFF5101 are separated by 37 nucleotides
GFF5098 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 199,699 to 200,502
GFF5098
GFF5099 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.52), at 200,514 to 201,335
GFF5099
GFF5100 - putative membrane protein, at 201,365 to 201,835
GFF5100
GFF5101 - Dihydroxy-acid dehydratase (EC 4.2.1.9), at 201,873 to 203,609
GFF5101
Position (kb) 201
202 Strain fitness (log2 ratio) -2
-1
0
1
2 at 200.598 kb on + strand, within GFF5099 at 200.790 kb on + strand, within GFF5099 at 200.790 kb on + strand, within GFF5099 at 200.790 kb on + strand, within GFF5099 at 200.791 kb on - strand, within GFF5099 at 200.946 kb on + strand, within GFF5099 at 200.946 kb on + strand, within GFF5099 at 200.946 kb on + strand, within GFF5099 at 200.946 kb on + strand, within GFF5099 at 200.946 kb on + strand, within GFF5099 at 200.946 kb on + strand, within GFF5099 at 200.947 kb on - strand, within GFF5099 at 200.947 kb on - strand, within GFF5099 at 200.947 kb on - strand, within GFF5099 at 201.294 kb on + strand at 201.294 kb on + strand at 201.294 kb on + strand at 201.294 kb on + strand at 201.294 kb on + strand at 201.294 kb on + strand at 201.295 kb on - strand at 201.330 kb on + strand at 201.330 kb on + strand at 201.331 kb on - strand at 201.331 kb on - strand at 201.497 kb on + strand, within GFF5100 at 201.497 kb on + strand, within GFF5100 at 201.542 kb on + strand, within GFF5100 at 201.542 kb on + strand, within GFF5100 at 201.543 kb on - strand, within GFF5100 at 201.543 kb on - strand, within GFF5100 at 201.543 kb on - strand, within GFF5100 at 201.543 kb on - strand, within GFF5100 at 201.543 kb on - strand, within GFF5100 at 201.543 kb on - strand, within GFF5100 at 201.543 kb on - strand, within GFF5100 at 201.543 kb on - strand, within GFF5100 at 201.543 kb on - strand, within GFF5100 at 201.557 kb on + strand, within GFF5100 at 201.680 kb on + strand, within GFF5100 at 201.680 kb on + strand, within GFF5100 at 202.089 kb on + strand, within GFF5101 at 202.089 kb on + strand, within GFF5101 at 202.089 kb on + strand, within GFF5101 at 202.090 kb on - strand, within GFF5101 at 202.090 kb on - strand, within GFF5101 at 202.123 kb on + strand, within GFF5101 at 202.123 kb on + strand, within GFF5101 at 202.124 kb on - strand, within GFF5101 at 202.395 kb on + strand, within GFF5101 at 202.395 kb on + strand, within GFF5101 at 202.395 kb on + strand, within GFF5101 at 202.395 kb on + strand, within GFF5101 at 202.395 kb on + strand, within GFF5101 at 202.396 kb on - strand, within GFF5101 at 202.396 kb on - strand, within GFF5101
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 200,598 + GFF5099 0.10 +0.9 200,790 + GFF5099 0.34 +1.2 200,790 + GFF5099 0.34 +0.2 200,790 + GFF5099 0.34 -0.4 200,791 - GFF5099 0.34 +0.7 200,946 + GFF5099 0.53 +0.2 200,946 + GFF5099 0.53 -0.1 200,946 + GFF5099 0.53 +0.6 200,946 + GFF5099 0.53 -0.1 200,946 + GFF5099 0.53 -0.3 200,946 + GFF5099 0.53 -0.8 200,947 - GFF5099 0.53 +1.6 200,947 - GFF5099 0.53 -0.8 200,947 - GFF5099 0.53 +0.1 201,294 + -0.1 201,294 + -0.2 201,294 + -0.7 201,294 + -1.1 201,294 + +1.5 201,294 + -0.3 201,295 - +0.1 201,330 + +0.1 201,330 + +1.0 201,331 - -0.3 201,331 - -0.4 201,497 + GFF5100 0.28 -1.3 201,497 + GFF5100 0.28 -0.4 201,542 + GFF5100 0.38 -2.1 201,542 + GFF5100 0.38 -0.2 201,543 - GFF5100 0.38 -0.2 201,543 - GFF5100 0.38 -0.0 201,543 - GFF5100 0.38 +0.7 201,543 - GFF5100 0.38 +0.8 201,543 - GFF5100 0.38 -1.0 201,543 - GFF5100 0.38 -0.3 201,543 - GFF5100 0.38 -0.2 201,543 - GFF5100 0.38 -0.2 201,543 - GFF5100 0.38 +0.0 201,557 + GFF5100 0.41 -0.7 201,680 + GFF5100 0.67 -0.4 201,680 + GFF5100 0.67 -0.5 202,089 + GFF5101 0.12 -0.7 202,089 + GFF5101 0.12 -0.4 202,089 + GFF5101 0.12 -0.6 202,090 - GFF5101 0.12 -1.7 202,090 - GFF5101 0.12 -1.2 202,123 + GFF5101 0.14 -0.5 202,123 + GFF5101 0.14 -0.5 202,124 - GFF5101 0.14 +0.8 202,395 + GFF5101 0.30 -0.8 202,395 + GFF5101 0.30 +0.3 202,395 + GFF5101 0.30 -0.8 202,395 + GFF5101 0.30 +0.5 202,395 + GFF5101 0.30 -0.7 202,396 - GFF5101 0.30 +2.6 202,396 - GFF5101 0.30 +0.8
Or see this region's nucleotide sequence