Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5094 and GFF5095 are separated by 3 nucleotides GFF5095 and GFF5096 are separated by 85 nucleotides GFF5096 and GFF5097 are separated by 13 nucleotides GFF5097 and GFF5098 are separated by 11 nucleotides
GFF5094 - BUG/TctC family periplasmic protein, at 197,046 to 198,014
GFF5094
GFF5095 - hypothetical protein, at 198,018 to 198,353
GFF5095
GFF5096 - similar to protocatechuate 4,5-dioxygenase beta chain, at 198,439 to 199,266
GFF5096
GFF5097 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 199,280 to 199,687
GFF5097
GFF5098 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 199,699 to 200,502
GFF5098
Position (kb) 198
199
200 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 197.593 kb on - strand, within GFF5094 at 197.593 kb on - strand, within GFF5094 at 197.770 kb on + strand, within GFF5094 at 197.771 kb on - strand, within GFF5094 at 197.771 kb on - strand, within GFF5094 at 197.970 kb on + strand at 198.183 kb on + strand, within GFF5095 at 198.183 kb on + strand, within GFF5095 at 198.183 kb on + strand, within GFF5095 at 198.183 kb on + strand, within GFF5095 at 198.184 kb on - strand, within GFF5095 at 198.184 kb on - strand, within GFF5095 at 198.252 kb on + strand, within GFF5095 at 198.285 kb on + strand, within GFF5095 at 198.286 kb on - strand, within GFF5095 at 198.286 kb on - strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.309 kb on + strand, within GFF5095 at 198.310 kb on - strand, within GFF5095 at 198.310 kb on - strand, within GFF5095 at 198.310 kb on - strand, within GFF5095 at 198.349 kb on + strand at 198.450 kb on - strand at 198.640 kb on + strand, within GFF5096 at 198.664 kb on + strand, within GFF5096 at 198.664 kb on + strand, within GFF5096 at 198.665 kb on - strand, within GFF5096 at 198.665 kb on - strand, within GFF5096 at 198.758 kb on + strand, within GFF5096 at 198.758 kb on + strand, within GFF5096 at 199.120 kb on + strand, within GFF5096 at 199.120 kb on + strand, within GFF5096 at 199.121 kb on - strand, within GFF5096 at 199.301 kb on + strand at 199.302 kb on - strand at 199.430 kb on + strand, within GFF5097 at 199.445 kb on + strand, within GFF5097 at 199.485 kb on - strand, within GFF5097 at 199.640 kb on + strand, within GFF5097 at 199.640 kb on + strand, within GFF5097 at 199.641 kb on - strand, within GFF5097 at 199.816 kb on + strand, within GFF5098 at 199.937 kb on - strand, within GFF5098 at 200.095 kb on + strand, within GFF5098 at 200.096 kb on - strand, within GFF5098
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 197,593 - GFF5094 0.56 -1.1 197,593 - GFF5094 0.56 +1.1 197,770 + GFF5094 0.75 -1.2 197,771 - GFF5094 0.75 -0.7 197,771 - GFF5094 0.75 +0.8 197,970 + -0.5 198,183 + GFF5095 0.49 -0.5 198,183 + GFF5095 0.49 +0.8 198,183 + GFF5095 0.49 -1.0 198,183 + GFF5095 0.49 +0.8 198,184 - GFF5095 0.49 -0.2 198,184 - GFF5095 0.49 +0.1 198,252 + GFF5095 0.70 -0.0 198,285 + GFF5095 0.79 -0.3 198,286 - GFF5095 0.80 -1.0 198,286 - GFF5095 0.80 +0.7 198,309 + GFF5095 0.87 +2.8 198,309 + GFF5095 0.87 +0.2 198,309 + GFF5095 0.87 -0.0 198,309 + GFF5095 0.87 -1.9 198,309 + GFF5095 0.87 -0.2 198,309 + GFF5095 0.87 +1.6 198,309 + GFF5095 0.87 +0.7 198,309 + GFF5095 0.87 +0.9 198,310 - GFF5095 0.87 -0.9 198,310 - GFF5095 0.87 +1.1 198,310 - GFF5095 0.87 -1.0 198,349 + -2.6 198,450 - -0.9 198,640 + GFF5096 0.24 -0.4 198,664 + GFF5096 0.27 -0.5 198,664 + GFF5096 0.27 -0.3 198,665 - GFF5096 0.27 -0.6 198,665 - GFF5096 0.27 -0.2 198,758 + GFF5096 0.39 +1.2 198,758 + GFF5096 0.39 -2.3 199,120 + GFF5096 0.82 +1.2 199,120 + GFF5096 0.82 +0.1 199,121 - GFF5096 0.82 -1.3 199,301 + -1.7 199,302 - -1.8 199,430 + GFF5097 0.37 -0.3 199,445 + GFF5097 0.40 -1.0 199,485 - GFF5097 0.50 -0.5 199,640 + GFF5097 0.88 -0.2 199,640 + GFF5097 0.88 -0.5 199,641 - GFF5097 0.88 -0.5 199,816 + GFF5098 0.15 -0.9 199,937 - GFF5098 0.30 +0.8 200,095 + GFF5098 0.49 +1.5 200,096 - GFF5098 0.49 +0.4
Or see this region's nucleotide sequence