Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5056 and GFF5057 are separated by 128 nucleotides GFF5057 and GFF5058 are separated by 54 nucleotides GFF5058 and GFF5059 overlap by 4 nucleotides
GFF5056 - Fumarylacetoacetase (EC 3.7.1.2), at 157,113 to 158,378
GFF5056
GFF5057 - Peptidoglycan D,D-transpeptidase MrdA (EC 3.4.16.4), at 158,507 to 160,480
GFF5057
GFF5058 - Rod shape-determining protein MreD, at 160,535 to 161,056
GFF5058
GFF5059 - Rod shape-determining protein MreC, at 161,053 to 162,012
GFF5059
Position (kb) 158
159
160
161 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 157.557 kb on + strand, within GFF5056 at 157.557 kb on + strand, within GFF5056 at 157.557 kb on + strand, within GFF5056 at 157.557 kb on + strand, within GFF5056 at 157.557 kb on + strand, within GFF5056 at 157.557 kb on + strand, within GFF5056 at 157.557 kb on + strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.558 kb on - strand, within GFF5056 at 157.695 kb on + strand, within GFF5056 at 157.695 kb on + strand, within GFF5056 at 157.695 kb on + strand, within GFF5056 at 157.695 kb on + strand, within GFF5056 at 157.696 kb on - strand, within GFF5056 at 157.696 kb on - strand, within GFF5056 at 157.833 kb on + strand, within GFF5056 at 157.833 kb on + strand, within GFF5056 at 157.833 kb on + strand, within GFF5056 at 157.834 kb on - strand, within GFF5056 at 157.834 kb on - strand, within GFF5056 at 157.834 kb on - strand, within GFF5056 at 158.100 kb on + strand, within GFF5056 at 158.100 kb on + strand, within GFF5056 at 158.101 kb on - strand, within GFF5056 at 158.371 kb on + strand at 158.371 kb on + strand at 158.371 kb on + strand at 158.372 kb on - strand at 158.508 kb on + strand at 158.508 kb on + strand at 158.509 kb on - strand at 158.509 kb on - strand at 158.509 kb on - strand at 158.509 kb on - strand at 158.518 kb on + strand at 158.518 kb on + strand at 158.518 kb on + strand at 158.518 kb on + strand at 158.518 kb on + strand at 158.518 kb on + strand at 158.518 kb on + strand at 158.519 kb on - strand at 158.519 kb on - strand at 158.519 kb on - strand at 158.519 kb on - strand at 158.519 kb on - strand at 158.653 kb on - strand at 158.653 kb on - strand at 158.653 kb on - strand at 158.808 kb on + strand, within GFF5057 at 158.808 kb on + strand, within GFF5057 at 158.808 kb on + strand, within GFF5057 at 158.808 kb on + strand, within GFF5057 at 158.808 kb on + strand, within GFF5057 at 158.808 kb on + strand, within GFF5057 at 158.808 kb on + strand, within GFF5057 at 158.808 kb on + strand, within GFF5057 at 158.808 kb on + strand, within GFF5057 at 158.808 kb on + strand, within GFF5057 at 158.808 kb on + strand, within GFF5057 at 158.808 kb on + strand, within GFF5057 at 158.808 kb on + strand, within GFF5057 at 158.809 kb on - strand, within GFF5057 at 158.809 kb on - strand, within GFF5057 at 158.809 kb on - strand, within GFF5057 at 158.809 kb on - strand, within GFF5057 at 158.809 kb on - strand, within GFF5057 at 158.809 kb on - strand, within GFF5057 at 158.809 kb on - strand, within GFF5057 at 158.809 kb on - strand, within GFF5057 at 158.809 kb on - strand, within GFF5057 at 158.809 kb on - strand, within GFF5057 at 158.809 kb on - strand, within GFF5057 at 158.809 kb on - strand, within GFF5057 at 158.809 kb on - strand, within GFF5057 at 158.809 kb on - strand, within GFF5057 at 158.809 kb on - strand, within GFF5057 at 158.809 kb on - strand, within GFF5057 at 158.809 kb on - strand, within GFF5057 at 158.869 kb on - strand, within GFF5057 at 158.869 kb on - strand, within GFF5057 at 158.869 kb on - strand, within GFF5057 at 158.899 kb on + strand, within GFF5057 at 158.958 kb on + strand, within GFF5057 at 158.959 kb on - strand, within GFF5057 at 159.111 kb on + strand, within GFF5057 at 159.111 kb on + strand, within GFF5057 at 159.111 kb on + strand, within GFF5057 at 159.111 kb on + strand, within GFF5057 at 159.111 kb on + strand, within GFF5057 at 159.111 kb on + strand, within GFF5057 at 159.111 kb on + strand, within GFF5057 at 159.112 kb on - strand, within GFF5057 at 159.112 kb on - strand, within GFF5057 at 159.112 kb on - strand, within GFF5057 at 159.112 kb on - strand, within GFF5057 at 159.112 kb on - strand, within GFF5057 at 159.112 kb on - strand, within GFF5057 at 159.112 kb on - strand, within GFF5057 at 159.112 kb on - strand, within GFF5057 at 159.112 kb on - strand, within GFF5057 at 159.150 kb on + strand, within GFF5057 at 159.150 kb on + strand, within GFF5057 at 159.150 kb on + strand, within GFF5057 at 159.150 kb on + strand, within GFF5057 at 159.151 kb on - strand, within GFF5057 at 159.151 kb on - strand, within GFF5057 at 159.151 kb on - strand, within GFF5057 at 159.151 kb on - strand, within GFF5057 at 159.177 kb on + strand, within GFF5057 at 159.177 kb on + strand, within GFF5057 at 159.177 kb on + strand, within GFF5057 at 159.177 kb on + strand, within GFF5057 at 159.178 kb on - strand, within GFF5057 at 159.178 kb on - strand, within GFF5057 at 159.178 kb on - strand, within GFF5057 at 159.178 kb on - strand, within GFF5057 at 159.178 kb on - strand, within GFF5057 at 159.316 kb on - strand, within GFF5057 at 159.321 kb on + strand, within GFF5057 at 159.321 kb on + strand, within GFF5057 at 159.321 kb on + strand, within GFF5057 at 159.321 kb on + strand, within GFF5057 at 159.321 kb on + strand, within GFF5057 at 159.321 kb on + strand, within GFF5057 at 159.321 kb on + strand, within GFF5057 at 159.321 kb on + strand, within GFF5057 at 159.321 kb on + strand, within GFF5057 at 159.321 kb on + strand, within GFF5057 at 159.321 kb on + strand, within GFF5057 at 159.321 kb on + strand, within GFF5057 at 159.321 kb on + strand, within GFF5057 at 159.321 kb on + strand, within GFF5057 at 159.321 kb on + strand, within GFF5057 at 159.321 kb on + strand, within GFF5057 at 159.322 kb on - strand, within GFF5057 at 159.322 kb on - strand, within GFF5057 at 159.322 kb on - strand, within GFF5057 at 159.322 kb on - strand, within GFF5057 at 159.322 kb on - strand, within GFF5057 at 159.322 kb on - strand, within GFF5057 at 159.322 kb on - strand, within GFF5057 at 159.322 kb on - strand, within GFF5057 at 159.322 kb on - strand, within GFF5057 at 159.322 kb on - strand, within GFF5057 at 159.322 kb on - strand, within GFF5057 at 159.322 kb on - strand, within GFF5057 at 159.322 kb on - strand, within GFF5057 at 159.415 kb on - strand, within GFF5057 at 159.489 kb on + strand, within GFF5057 at 159.489 kb on + strand, within GFF5057 at 159.489 kb on + strand, within GFF5057 at 159.489 kb on + strand, within GFF5057 at 159.489 kb on + strand, within GFF5057 at 159.489 kb on + strand, within GFF5057 at 159.489 kb on + strand, within GFF5057 at 159.489 kb on + strand, within GFF5057 at 159.489 kb on + strand, within GFF5057 at 159.489 kb on + strand, within GFF5057 at 159.489 kb on + strand, within GFF5057 at 159.490 kb on - strand, within GFF5057 at 159.490 kb on - strand, within GFF5057 at 159.490 kb on - strand, within GFF5057 at 159.490 kb on - strand, within GFF5057 at 159.490 kb on - strand, within GFF5057 at 159.490 kb on - strand, within GFF5057 at 159.490 kb on - strand, within GFF5057 at 159.490 kb on - strand, within GFF5057 at 159.490 kb on - strand, within GFF5057 at 159.490 kb on - strand, within GFF5057 at 159.490 kb on - strand, within GFF5057 at 159.490 kb on - strand, within GFF5057 at 159.490 kb on - strand, within GFF5057 at 159.490 kb on - strand, within GFF5057 at 159.490 kb on - strand, within GFF5057 at 159.507 kb on + strand, within GFF5057 at 159.508 kb on - strand, within GFF5057 at 159.508 kb on - strand, within GFF5057 at 159.508 kb on - strand, within GFF5057 at 159.873 kb on + strand, within GFF5057 at 159.888 kb on + strand, within GFF5057 at 159.888 kb on + strand, within GFF5057 at 159.888 kb on + strand, within GFF5057 at 159.888 kb on + strand, within GFF5057 at 159.888 kb on + strand, within GFF5057 at 159.888 kb on + strand, within GFF5057 at 159.888 kb on + strand, within GFF5057 at 159.888 kb on + strand, within GFF5057 at 159.888 kb on + strand, within GFF5057 at 159.888 kb on + strand, within GFF5057 at 159.888 kb on + strand, within GFF5057 at 159.888 kb on + strand, within GFF5057 at 159.888 kb on + strand, within GFF5057 at 159.888 kb on + strand, within GFF5057 at 159.957 kb on + strand, within GFF5057 at 159.957 kb on + strand, within GFF5057 at 159.957 kb on + strand, within GFF5057 at 159.957 kb on + strand, within GFF5057 at 159.957 kb on + strand, within GFF5057 at 159.957 kb on + strand, within GFF5057 at 159.993 kb on + strand, within GFF5057 at 159.993 kb on + strand, within GFF5057 at 159.993 kb on + strand, within GFF5057 at 159.994 kb on - strand, within GFF5057 at 160.233 kb on + strand, within GFF5057 at 160.234 kb on - strand, within GFF5057 at 160.234 kb on - strand, within GFF5057 at 160.242 kb on + strand, within GFF5057 at 160.242 kb on + strand, within GFF5057 at 160.242 kb on + strand, within GFF5057 at 160.242 kb on + strand, within GFF5057 at 160.243 kb on - strand, within GFF5057 at 160.243 kb on - strand, within GFF5057 at 160.243 kb on - strand, within GFF5057 at 160.243 kb on - strand, within GFF5057 at 160.368 kb on + strand at 160.368 kb on + strand at 160.368 kb on + strand at 160.368 kb on + strand at 160.368 kb on + strand at 160.368 kb on + strand at 160.368 kb on + strand at 160.368 kb on + strand at 160.369 kb on - strand at 160.369 kb on - strand at 160.369 kb on - strand at 160.369 kb on - strand at 160.369 kb on - strand at 160.369 kb on - strand at 160.369 kb on - strand at 160.369 kb on - strand at 160.494 kb on + strand at 160.494 kb on + strand at 160.494 kb on + strand at 160.494 kb on + strand at 160.494 kb on + strand at 160.494 kb on + strand at 160.495 kb on - strand at 160.495 kb on - strand at 160.495 kb on - strand at 160.495 kb on - strand at 160.536 kb on + strand at 160.536 kb on + strand at 160.537 kb on - strand at 160.537 kb on - strand at 160.537 kb on - strand at 160.537 kb on - strand at 160.586 kb on + strand at 160.586 kb on + strand at 160.586 kb on + strand at 160.587 kb on - strand at 160.587 kb on - strand at 160.587 kb on - strand at 160.587 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 157,557 + GFF5056 0.35 -0.0 157,557 + GFF5056 0.35 -0.0 157,557 + GFF5056 0.35 -2.0 157,557 + GFF5056 0.35 +0.5 157,557 + GFF5056 0.35 +1.0 157,557 + GFF5056 0.35 -0.5 157,557 + GFF5056 0.35 -0.3 157,558 - GFF5056 0.35 -0.3 157,558 - GFF5056 0.35 +0.2 157,558 - GFF5056 0.35 +0.8 157,558 - GFF5056 0.35 +0.1 157,558 - GFF5056 0.35 -1.9 157,558 - GFF5056 0.35 +0.8 157,558 - GFF5056 0.35 -0.2 157,558 - GFF5056 0.35 +0.0 157,558 - GFF5056 0.35 -0.9 157,558 - GFF5056 0.35 -0.3 157,558 - GFF5056 0.35 -1.2 157,558 - GFF5056 0.35 -0.5 157,558 - GFF5056 0.35 -0.0 157,558 - GFF5056 0.35 -0.4 157,558 - GFF5056 0.35 -2.2 157,558 - GFF5056 0.35 +0.2 157,558 - GFF5056 0.35 -0.2 157,558 - GFF5056 0.35 -0.5 157,695 + GFF5056 0.46 +0.2 157,695 + GFF5056 0.46 -0.9 157,695 + GFF5056 0.46 +0.8 157,695 + GFF5056 0.46 -2.3 157,696 - GFF5056 0.46 +0.4 157,696 - GFF5056 0.46 -0.5 157,833 + GFF5056 0.57 +0.1 157,833 + GFF5056 0.57 -0.4 157,833 + GFF5056 0.57 -0.5 157,834 - GFF5056 0.57 -0.5 157,834 - GFF5056 0.57 +1.2 157,834 - GFF5056 0.57 -0.9 158,100 + GFF5056 0.78 -0.5 158,100 + GFF5056 0.78 -1.7 158,101 - GFF5056 0.78 -1.0 158,371 + +0.3 158,371 + +0.8 158,371 + -0.8 158,372 - +0.0 158,508 + +0.2 158,508 + +0.3 158,509 - -0.4 158,509 - +0.7 158,509 - -0.2 158,509 - -0.4 158,518 + +0.1 158,518 + -0.8 158,518 + -0.1 158,518 + +1.0 158,518 + +0.7 158,518 + +0.6 158,518 + -0.2 158,519 - -0.5 158,519 - -1.5 158,519 - -2.2 158,519 - -0.5 158,519 - -0.0 158,653 - -1.3 158,653 - -1.6 158,653 - -1.3 158,808 + GFF5057 0.15 -1.1 158,808 + GFF5057 0.15 -1.0 158,808 + GFF5057 0.15 -1.6 158,808 + GFF5057 0.15 -2.1 158,808 + GFF5057 0.15 -1.6 158,808 + GFF5057 0.15 -1.2 158,808 + GFF5057 0.15 -1.3 158,808 + GFF5057 0.15 -2.4 158,808 + GFF5057 0.15 -1.3 158,808 + GFF5057 0.15 -0.6 158,808 + GFF5057 0.15 -2.4 158,808 + GFF5057 0.15 -0.3 158,808 + GFF5057 0.15 -1.6 158,809 - GFF5057 0.15 -2.8 158,809 - GFF5057 0.15 -1.5 158,809 - GFF5057 0.15 -0.5 158,809 - GFF5057 0.15 -2.3 158,809 - GFF5057 0.15 -0.8 158,809 - GFF5057 0.15 +0.2 158,809 - GFF5057 0.15 -1.3 158,809 - GFF5057 0.15 -1.6 158,809 - GFF5057 0.15 -1.2 158,809 - GFF5057 0.15 -2.5 158,809 - GFF5057 0.15 -2.1 158,809 - GFF5057 0.15 -0.7 158,809 - GFF5057 0.15 -1.3 158,809 - GFF5057 0.15 -1.2 158,809 - GFF5057 0.15 -2.0 158,809 - GFF5057 0.15 -1.9 158,809 - GFF5057 0.15 -0.4 158,869 - GFF5057 0.18 -0.8 158,869 - GFF5057 0.18 -2.1 158,869 - GFF5057 0.18 -2.0 158,899 + GFF5057 0.20 -1.6 158,958 + GFF5057 0.23 -1.3 158,959 - GFF5057 0.23 -2.2 159,111 + GFF5057 0.31 -1.8 159,111 + GFF5057 0.31 -1.6 159,111 + GFF5057 0.31 -1.6 159,111 + GFF5057 0.31 -1.2 159,111 + GFF5057 0.31 -0.2 159,111 + GFF5057 0.31 -1.5 159,111 + GFF5057 0.31 -1.5 159,112 - GFF5057 0.31 +0.8 159,112 - GFF5057 0.31 +1.2 159,112 - GFF5057 0.31 +0.5 159,112 - GFF5057 0.31 -0.5 159,112 - GFF5057 0.31 -2.2 159,112 - GFF5057 0.31 -1.8 159,112 - GFF5057 0.31 -1.2 159,112 - GFF5057 0.31 -2.0 159,112 - GFF5057 0.31 -1.4 159,150 + GFF5057 0.33 -3.0 159,150 + GFF5057 0.33 -1.3 159,150 + GFF5057 0.33 -0.2 159,150 + GFF5057 0.33 +0.1 159,151 - GFF5057 0.33 -2.7 159,151 - GFF5057 0.33 -1.6 159,151 - GFF5057 0.33 -1.0 159,151 - GFF5057 0.33 -1.3 159,177 + GFF5057 0.34 -2.1 159,177 + GFF5057 0.34 -0.9 159,177 + GFF5057 0.34 -1.2 159,177 + GFF5057 0.34 -1.7 159,178 - GFF5057 0.34 -2.5 159,178 - GFF5057 0.34 -1.2 159,178 - GFF5057 0.34 -2.6 159,178 - GFF5057 0.34 -2.3 159,178 - GFF5057 0.34 -2.1 159,316 - GFF5057 0.41 -2.1 159,321 + GFF5057 0.41 -1.8 159,321 + GFF5057 0.41 -1.9 159,321 + GFF5057 0.41 -1.2 159,321 + GFF5057 0.41 -1.2 159,321 + GFF5057 0.41 -1.8 159,321 + GFF5057 0.41 -0.2 159,321 + GFF5057 0.41 -0.4 159,321 + GFF5057 0.41 -3.5 159,321 + GFF5057 0.41 -1.4 159,321 + GFF5057 0.41 -0.9 159,321 + GFF5057 0.41 -0.9 159,321 + GFF5057 0.41 -1.8 159,321 + GFF5057 0.41 -1.6 159,321 + GFF5057 0.41 -1.3 159,321 + GFF5057 0.41 -0.5 159,321 + GFF5057 0.41 -1.9 159,322 - GFF5057 0.41 -2.5 159,322 - GFF5057 0.41 -0.3 159,322 - GFF5057 0.41 -1.5 159,322 - GFF5057 0.41 +0.4 159,322 - GFF5057 0.41 -1.6 159,322 - GFF5057 0.41 -1.9 159,322 - GFF5057 0.41 -2.4 159,322 - GFF5057 0.41 -1.6 159,322 - GFF5057 0.41 -1.5 159,322 - GFF5057 0.41 -2.8 159,322 - GFF5057 0.41 -0.3 159,322 - GFF5057 0.41 -3.0 159,322 - GFF5057 0.41 -1.5 159,415 - GFF5057 0.46 -1.7 159,489 + GFF5057 0.50 -2.1 159,489 + GFF5057 0.50 -0.7 159,489 + GFF5057 0.50 -1.8 159,489 + GFF5057 0.50 -1.6 159,489 + GFF5057 0.50 -1.2 159,489 + GFF5057 0.50 +0.4 159,489 + GFF5057 0.50 -3.5 159,489 + GFF5057 0.50 -0.3 159,489 + GFF5057 0.50 -1.5 159,489 + GFF5057 0.50 -1.0 159,489 + GFF5057 0.50 -1.2 159,490 - GFF5057 0.50 -0.6 159,490 - GFF5057 0.50 -1.5 159,490 - GFF5057 0.50 -1.4 159,490 - GFF5057 0.50 -0.2 159,490 - GFF5057 0.50 -0.5 159,490 - GFF5057 0.50 -0.9 159,490 - GFF5057 0.50 -2.2 159,490 - GFF5057 0.50 -2.1 159,490 - GFF5057 0.50 -0.8 159,490 - GFF5057 0.50 -3.1 159,490 - GFF5057 0.50 -1.6 159,490 - GFF5057 0.50 -2.6 159,490 - GFF5057 0.50 -2.2 159,490 - GFF5057 0.50 +0.2 159,490 - GFF5057 0.50 -2.5 159,507 + GFF5057 0.51 -0.8 159,508 - GFF5057 0.51 -1.8 159,508 - GFF5057 0.51 -1.3 159,508 - GFF5057 0.51 -2.0 159,873 + GFF5057 0.69 -1.5 159,888 + GFF5057 0.70 -0.8 159,888 + GFF5057 0.70 -0.8 159,888 + GFF5057 0.70 -0.9 159,888 + GFF5057 0.70 -0.5 159,888 + GFF5057 0.70 -2.1 159,888 + GFF5057 0.70 -0.6 159,888 + GFF5057 0.70 -1.3 159,888 + GFF5057 0.70 -1.0 159,888 + GFF5057 0.70 -1.4 159,888 + GFF5057 0.70 -1.0 159,888 + GFF5057 0.70 -0.7 159,888 + GFF5057 0.70 -2.6 159,888 + GFF5057 0.70 -3.1 159,888 + GFF5057 0.70 +0.2 159,957 + GFF5057 0.73 -1.2 159,957 + GFF5057 0.73 -2.4 159,957 + GFF5057 0.73 -2.0 159,957 + GFF5057 0.73 -1.3 159,957 + GFF5057 0.73 -2.2 159,957 + GFF5057 0.73 +0.4 159,993 + GFF5057 0.75 -3.2 159,993 + GFF5057 0.75 -2.1 159,993 + GFF5057 0.75 -1.2 159,994 - GFF5057 0.75 -0.2 160,233 + GFF5057 0.87 -1.5 160,234 - GFF5057 0.87 -2.4 160,234 - GFF5057 0.87 -0.4 160,242 + GFF5057 0.88 -1.4 160,242 + GFF5057 0.88 -1.1 160,242 + GFF5057 0.88 -0.9 160,242 + GFF5057 0.88 +0.6 160,243 - GFF5057 0.88 -1.8 160,243 - GFF5057 0.88 +0.4 160,243 - GFF5057 0.88 -1.7 160,243 - GFF5057 0.88 -0.8 160,368 + -1.7 160,368 + -2.0 160,368 + -2.6 160,368 + -2.6 160,368 + -1.1 160,368 + -1.0 160,368 + -1.6 160,368 + -0.8 160,369 - -0.8 160,369 - -1.0 160,369 - -1.3 160,369 - -1.6 160,369 - -1.7 160,369 - -1.6 160,369 - -1.2 160,369 - -0.4 160,494 + -0.7 160,494 + -0.1 160,494 + +0.1 160,494 + +1.5 160,494 + +0.5 160,494 + -0.6 160,495 - -0.2 160,495 - -0.8 160,495 - -0.5 160,495 - +0.8 160,536 + +0.0 160,536 + +0.4 160,537 - -0.7 160,537 - +0.7 160,537 - -0.5 160,537 - -1.9 160,586 + -2.3 160,586 + -0.3 160,586 + -1.2 160,587 - -0.6 160,587 - -0.8 160,587 - +0.3 160,587 - -0.5
Or see this region's nucleotide sequence