Strain Fitness in Variovorax sp. SCN45 around GFF4974

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4972 and GFF4973 overlap by 4 nucleotidesGFF4973 and GFF4974 overlap by 4 nucleotidesGFF4974 and GFF4975 are separated by 13 nucleotides GFF4972 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 72,473 to 73,237 GFF4972 GFF4973 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 73,234 to 74,217 GFF4973 GFF4974 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 74,214 to 75,116 GFF4974 GFF4975 - Branched-chain amino acid ABC transporter, amino acid-binding protein (TC 3.A.1.4.1), at 75,130 to 76,347 GFF4975 Position (kb) 74 75 76Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 73.352 kb on + strand, within GFF4973at 73.352 kb on + strand, within GFF4973at 73.352 kb on + strand, within GFF4973at 73.353 kb on - strand, within GFF4973at 73.532 kb on + strand, within GFF4973at 73.532 kb on + strand, within GFF4973at 73.532 kb on + strand, within GFF4973at 73.533 kb on - strand, within GFF4973at 73.533 kb on - strand, within GFF4973at 73.533 kb on - strand, within GFF4973at 73.533 kb on - strand, within GFF4973at 73.533 kb on - strand, within GFF4973at 73.727 kb on + strand, within GFF4973at 73.727 kb on + strand, within GFF4973at 73.754 kb on + strand, within GFF4973at 73.754 kb on + strand, within GFF4973at 73.871 kb on + strand, within GFF4973at 73.871 kb on + strand, within GFF4973at 73.871 kb on + strand, within GFF4973at 73.872 kb on - strand, within GFF4973at 73.872 kb on - strand, within GFF4973at 73.997 kb on + strand, within GFF4973at 73.997 kb on + strand, within GFF4973at 74.111 kb on + strand, within GFF4973at 74.111 kb on + strand, within GFF4973at 74.111 kb on + strand, within GFF4973at 74.111 kb on + strand, within GFF4973at 74.112 kb on - strand, within GFF4973at 74.112 kb on - strand, within GFF4973at 74.112 kb on - strand, within GFF4973at 74.120 kb on + strandat 74.218 kb on - strandat 74.218 kb on - strandat 74.623 kb on + strand, within GFF4974at 74.624 kb on - strand, within GFF4974at 74.624 kb on - strand, within GFF4974at 74.782 kb on + strand, within GFF4974at 74.917 kb on + strand, within GFF4974at 75.047 kb on - strandat 75.140 kb on + strandat 75.140 kb on + strandat 75.140 kb on + strandat 75.141 kb on - strandat 75.141 kb on - strandat 75.141 kb on - strandat 75.141 kb on - strandat 75.141 kb on - strandat 75.141 kb on - strandat 75.158 kb on + strandat 75.158 kb on + strandat 75.159 kb on - strandat 75.203 kb on + strandat 75.203 kb on + strandat 75.203 kb on + strandat 75.203 kb on + strandat 75.203 kb on + strandat 75.203 kb on + strandat 75.204 kb on - strandat 75.204 kb on - strandat 75.204 kb on - strandat 75.204 kb on - strandat 75.204 kb on - strandat 75.204 kb on - strandat 75.204 kb on - strandat 75.204 kb on - strandat 75.383 kb on + strand, within GFF4975at 75.383 kb on + strand, within GFF4975at 75.383 kb on + strand, within GFF4975at 75.383 kb on + strand, within GFF4975at 75.383 kb on + strand, within GFF4975at 75.383 kb on + strand, within GFF4975at 75.383 kb on + strand, within GFF4975at 75.384 kb on - strand, within GFF4975at 75.384 kb on - strand, within GFF4975at 75.384 kb on - strand, within GFF4975at 75.384 kb on - strand, within GFF4975at 75.384 kb on - strand, within GFF4975at 75.384 kb on - strand, within GFF4975at 75.384 kb on - strand, within GFF4975at 75.434 kb on + strand, within GFF4975at 75.434 kb on + strand, within GFF4975at 75.434 kb on + strand, within GFF4975at 75.434 kb on + strand, within GFF4975at 75.434 kb on + strand, within GFF4975at 75.434 kb on + strand, within GFF4975at 75.434 kb on + strand, within GFF4975at 75.434 kb on + strand, within GFF4975at 75.434 kb on + strand, within GFF4975at 75.434 kb on + strand, within GFF4975at 75.434 kb on + strand, within GFF4975at 75.434 kb on + strand, within GFF4975at 75.434 kb on + strand, within GFF4975at 75.435 kb on - strand, within GFF4975at 75.435 kb on - strand, within GFF4975at 75.435 kb on - strand, within GFF4975at 75.435 kb on - strand, within GFF4975at 75.443 kb on + strand, within GFF4975at 75.443 kb on + strand, within GFF4975at 75.444 kb on - strand, within GFF4975at 75.444 kb on - strand, within GFF4975at 75.444 kb on - strand, within GFF4975at 75.497 kb on + strand, within GFF4975at 75.497 kb on + strand, within GFF4975at 75.497 kb on + strand, within GFF4975at 75.497 kb on + strand, within GFF4975at 75.497 kb on + strand, within GFF4975at 75.497 kb on + strand, within GFF4975at 75.497 kb on + strand, within GFF4975at 75.497 kb on + strand, within GFF4975at 75.498 kb on - strand, within GFF4975at 75.498 kb on - strand, within GFF4975at 75.498 kb on - strand, within GFF4975at 75.498 kb on - strand, within GFF4975at 75.498 kb on - strand, within GFF4975at 75.638 kb on + strand, within GFF4975at 75.638 kb on + strand, within GFF4975at 75.638 kb on + strand, within GFF4975at 75.638 kb on + strand, within GFF4975at 75.639 kb on - strand, within GFF4975at 75.639 kb on - strand, within GFF4975at 75.639 kb on - strand, within GFF4975at 75.639 kb on - strand, within GFF4975at 75.639 kb on - strand, within GFF4975at 75.639 kb on - strand, within GFF4975at 75.815 kb on + strand, within GFF4975at 75.815 kb on + strand, within GFF4975at 75.902 kb on + strand, within GFF4975at 75.902 kb on + strand, within GFF4975at 75.902 kb on + strand, within GFF4975at 75.902 kb on + strand, within GFF4975at 75.902 kb on + strand, within GFF4975at 75.903 kb on - strand, within GFF4975at 75.908 kb on + strand, within GFF4975at 75.908 kb on + strand, within GFF4975at 75.908 kb on + strand, within GFF4975at 75.908 kb on + strand, within GFF4975at 75.908 kb on + strand, within GFF4975at 75.908 kb on + strand, within GFF4975at 75.909 kb on - strand, within GFF4975at 75.909 kb on - strand, within GFF4975at 75.909 kb on - strand, within GFF4975at 75.909 kb on - strand, within GFF4975at 75.909 kb on - strand, within GFF4975at 75.983 kb on + strand, within GFF4975at 75.984 kb on - strand, within GFF4975

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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73,352 + GFF4973 0.12 +0.4
73,352 + GFF4973 0.12 -0.3
73,352 + GFF4973 0.12 -0.3
73,353 - GFF4973 0.12 -0.3
73,532 + GFF4973 0.30 -1.6
73,532 + GFF4973 0.30 +0.4
73,532 + GFF4973 0.30 +2.8
73,533 - GFF4973 0.30 +0.7
73,533 - GFF4973 0.30 -0.4
73,533 - GFF4973 0.30 -1.1
73,533 - GFF4973 0.30 -0.1
73,533 - GFF4973 0.30 +0.1
73,727 + GFF4973 0.50 -0.5
73,727 + GFF4973 0.50 +1.6
73,754 + GFF4973 0.53 -1.0
73,754 + GFF4973 0.53 -2.6
73,871 + GFF4973 0.65 -1.2
73,871 + GFF4973 0.65 -0.6
73,871 + GFF4973 0.65 +0.7
73,872 - GFF4973 0.65 +1.8
73,872 - GFF4973 0.65 -1.3
73,997 + GFF4973 0.78 +0.3
73,997 + GFF4973 0.78 +0.1
74,111 + GFF4973 0.89 -0.6
74,111 + GFF4973 0.89 +0.1
74,111 + GFF4973 0.89 -0.7
74,111 + GFF4973 0.89 -0.4
74,112 - GFF4973 0.89 -0.2
74,112 - GFF4973 0.89 -0.5
74,112 - GFF4973 0.89 +1.1
74,120 + -0.8
74,218 - +0.1
74,218 - +0.1
74,623 + GFF4974 0.45 -0.7
74,624 - GFF4974 0.45 -0.8
74,624 - GFF4974 0.45 -0.2
74,782 + GFF4974 0.63 -1.0
74,917 + GFF4974 0.78 -0.3
75,047 - -0.7
75,140 + -0.8
75,140 + +0.3
75,140 + -0.6
75,141 - +0.8
75,141 - -0.6
75,141 - +0.2
75,141 - -0.7
75,141 - -1.5
75,141 - -1.3
75,158 + +2.1
75,158 + -0.9
75,159 - +0.0
75,203 + -0.4
75,203 + +0.2
75,203 + -0.8
75,203 + -2.4
75,203 + -1.4
75,203 + +0.8
75,204 - -1.7
75,204 - +0.2
75,204 - +0.8
75,204 - -1.9
75,204 - -0.3
75,204 - +0.4
75,204 - -0.7
75,204 - -0.9
75,383 + GFF4975 0.21 -0.9
75,383 + GFF4975 0.21 -1.1
75,383 + GFF4975 0.21 +0.4
75,383 + GFF4975 0.21 +0.3
75,383 + GFF4975 0.21 -0.5
75,383 + GFF4975 0.21 -0.2
75,383 + GFF4975 0.21 +0.6
75,384 - GFF4975 0.21 -2.8
75,384 - GFF4975 0.21 +0.8
75,384 - GFF4975 0.21 -0.4
75,384 - GFF4975 0.21 -0.4
75,384 - GFF4975 0.21 +0.3
75,384 - GFF4975 0.21 -1.0
75,384 - GFF4975 0.21 -0.4
75,434 + GFF4975 0.25 -1.0
75,434 + GFF4975 0.25 -0.1
75,434 + GFF4975 0.25 -1.0
75,434 + GFF4975 0.25 +1.1
75,434 + GFF4975 0.25 -0.2
75,434 + GFF4975 0.25 +0.2
75,434 + GFF4975 0.25 -1.3
75,434 + GFF4975 0.25 +0.2
75,434 + GFF4975 0.25 -0.4
75,434 + GFF4975 0.25 +0.8
75,434 + GFF4975 0.25 -0.2
75,434 + GFF4975 0.25 -0.6
75,434 + GFF4975 0.25 -1.0
75,435 - GFF4975 0.25 -0.0
75,435 - GFF4975 0.25 -0.8
75,435 - GFF4975 0.25 -0.4
75,435 - GFF4975 0.25 +0.2
75,443 + GFF4975 0.26 +0.2
75,443 + GFF4975 0.26 +0.1
75,444 - GFF4975 0.26 +0.6
75,444 - GFF4975 0.26 +1.1
75,444 - GFF4975 0.26 -0.5
75,497 + GFF4975 0.30 +1.2
75,497 + GFF4975 0.30 -0.6
75,497 + GFF4975 0.30 +0.3
75,497 + GFF4975 0.30 +0.1
75,497 + GFF4975 0.30 -0.7
75,497 + GFF4975 0.30 -0.1
75,497 + GFF4975 0.30 -0.6
75,497 + GFF4975 0.30 -0.1
75,498 - GFF4975 0.30 -0.1
75,498 - GFF4975 0.30 +0.7
75,498 - GFF4975 0.30 +0.8
75,498 - GFF4975 0.30 -0.2
75,498 - GFF4975 0.30 +0.9
75,638 + GFF4975 0.42 +2.1
75,638 + GFF4975 0.42 +1.8
75,638 + GFF4975 0.42 +0.2
75,638 + GFF4975 0.42 -1.1
75,639 - GFF4975 0.42 -0.3
75,639 - GFF4975 0.42 +0.2
75,639 - GFF4975 0.42 +0.5
75,639 - GFF4975 0.42 -1.0
75,639 - GFF4975 0.42 +0.8
75,639 - GFF4975 0.42 +0.0
75,815 + GFF4975 0.56 +0.2
75,815 + GFF4975 0.56 -0.4
75,902 + GFF4975 0.63 -0.3
75,902 + GFF4975 0.63 -0.2
75,902 + GFF4975 0.63 -2.3
75,902 + GFF4975 0.63 -0.1
75,902 + GFF4975 0.63 -0.6
75,903 - GFF4975 0.63 +0.8
75,908 + GFF4975 0.64 -0.2
75,908 + GFF4975 0.64 +0.7
75,908 + GFF4975 0.64 -0.4
75,908 + GFF4975 0.64 +0.9
75,908 + GFF4975 0.64 -0.2
75,908 + GFF4975 0.64 -1.0
75,909 - GFF4975 0.64 +1.8
75,909 - GFF4975 0.64 +0.7
75,909 - GFF4975 0.64 +0.4
75,909 - GFF4975 0.64 -0.2
75,909 - GFF4975 0.64 -0.6
75,983 + GFF4975 0.70 +0.6
75,984 - GFF4975 0.70 +0.8

Or see this region's nucleotide sequence