Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4968 and GFF4969 overlap by 1 nucleotides GFF4969 and GFF4970 are separated by 24 nucleotides GFF4970 and GFF4971 are separated by 24 nucleotides GFF4971 and GFF4972 overlap by 8 nucleotides
GFF4968 - Succinate dehydrogenase/fumarate reductase, flavoprotein subunit, at 68,495 to 70,219
GFF4968
GFF4969 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 70,219 to 70,962
GFF4969
GFF4970 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 70,987 to 71,736
GFF4970
GFF4971 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 71,761 to 72,480
GFF4971
GFF4972 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 72,473 to 73,237
GFF4972
Position (kb) 70
71
72 Strain fitness (log2 ratio) -2
-1
0
1 at 70.484 kb on + strand, within GFF4969 at 70.514 kb on + strand, within GFF4969 at 70.514 kb on + strand, within GFF4969 at 70.514 kb on + strand, within GFF4969 at 70.515 kb on - strand, within GFF4969 at 70.515 kb on - strand, within GFF4969 at 70.637 kb on + strand, within GFF4969 at 70.637 kb on + strand, within GFF4969 at 70.637 kb on + strand, within GFF4969 at 70.844 kb on + strand, within GFF4969 at 70.844 kb on + strand, within GFF4969 at 71.036 kb on + strand at 71.036 kb on + strand at 71.036 kb on + strand at 71.036 kb on + strand at 71.036 kb on + strand at 71.037 kb on - strand at 71.037 kb on - strand at 71.037 kb on - strand at 71.040 kb on + strand at 71.041 kb on - strand at 71.041 kb on - strand at 71.041 kb on - strand at 71.121 kb on + strand, within GFF4970 at 71.121 kb on + strand, within GFF4970 at 71.121 kb on + strand, within GFF4970 at 71.122 kb on - strand, within GFF4970 at 71.122 kb on - strand, within GFF4970 at 71.122 kb on - strand, within GFF4970 at 71.123 kb on + strand, within GFF4970 at 71.123 kb on + strand, within GFF4970 at 71.123 kb on + strand, within GFF4970 at 71.123 kb on + strand, within GFF4970 at 71.124 kb on - strand, within GFF4970 at 71.124 kb on - strand, within GFF4970 at 71.277 kb on - strand, within GFF4970 at 71.277 kb on - strand, within GFF4970 at 71.853 kb on - strand, within GFF4971 at 71.853 kb on - strand, within GFF4971 at 71.858 kb on + strand, within GFF4971 at 71.859 kb on - strand, within GFF4971 at 71.859 kb on - strand, within GFF4971 at 72.117 kb on - strand, within GFF4971 at 72.117 kb on - strand, within GFF4971 at 72.165 kb on - strand, within GFF4971 at 72.165 kb on - strand, within GFF4971 at 72.165 kb on - strand, within GFF4971 at 72.438 kb on - strand at 72.501 kb on + strand at 72.501 kb on + strand at 72.502 kb on - strand at 72.503 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 70,484 + GFF4969 0.36 +1.5 70,514 + GFF4969 0.40 -0.2 70,514 + GFF4969 0.40 -1.0 70,514 + GFF4969 0.40 +0.0 70,515 - GFF4969 0.40 +0.0 70,515 - GFF4969 0.40 -1.2 70,637 + GFF4969 0.56 -0.8 70,637 + GFF4969 0.56 +0.1 70,637 + GFF4969 0.56 -0.6 70,844 + GFF4969 0.84 +1.2 70,844 + GFF4969 0.84 +1.2 71,036 + -0.8 71,036 + +0.3 71,036 + +0.3 71,036 + +0.1 71,036 + +0.0 71,037 - +0.6 71,037 - -0.8 71,037 - +0.8 71,040 + -0.2 71,041 - -0.1 71,041 - +0.5 71,041 - -0.7 71,121 + GFF4970 0.18 +0.6 71,121 + GFF4970 0.18 +0.4 71,121 + GFF4970 0.18 +0.0 71,122 - GFF4970 0.18 +0.1 71,122 - GFF4970 0.18 +1.6 71,122 - GFF4970 0.18 +0.4 71,123 + GFF4970 0.18 -0.2 71,123 + GFF4970 0.18 +0.6 71,123 + GFF4970 0.18 +0.1 71,123 + GFF4970 0.18 -0.3 71,124 - GFF4970 0.18 +0.2 71,124 - GFF4970 0.18 -2.7 71,277 - GFF4970 0.39 -0.2 71,277 - GFF4970 0.39 -0.3 71,853 - GFF4971 0.13 -0.7 71,853 - GFF4971 0.13 -0.6 71,858 + GFF4971 0.13 +0.5 71,859 - GFF4971 0.14 -0.8 71,859 - GFF4971 0.14 +0.1 72,117 - GFF4971 0.49 +0.2 72,117 - GFF4971 0.49 +1.2 72,165 - GFF4971 0.56 -0.4 72,165 - GFF4971 0.56 -0.2 72,165 - GFF4971 0.56 +1.3 72,438 - +0.5 72,501 + +0.4 72,501 + -0.3 72,502 - +0.8 72,503 - -0.4
Or see this region's nucleotide sequence