Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4965 and GFF4966 are separated by 74 nucleotides GFF4966 and GFF4967 are separated by 523 nucleotides GFF4967 and GFF4968 are separated by 19 nucleotides
GFF4965 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 66,283 to 66,996
GFF4965
GFF4966 - no description, at 67,071 to 67,343
GFF4966
GFF4967 - Glutathione S-transferase family protein, at 67,867 to 68,475
GFF4967
GFF4968 - Succinate dehydrogenase/fumarate reductase, flavoprotein subunit, at 68,495 to 70,219
GFF4968
Position (kb)
67
68
69 Strain fitness (log2 ratio)
-1
0
1
2 at 67.031 kb on + strand at 67.031 kb on + strand at 67.174 kb on + strand, within GFF4966 at 67.174 kb on + strand, within GFF4966 at 67.175 kb on - strand, within GFF4966 at 67.175 kb on - strand, within GFF4966 at 67.175 kb on - strand, within GFF4966 at 67.337 kb on + strand at 67.338 kb on - strand at 67.428 kb on + strand at 67.428 kb on + strand at 67.428 kb on + strand at 67.429 kb on - strand at 67.429 kb on - strand at 67.429 kb on - strand at 67.429 kb on - strand at 67.450 kb on + strand at 67.451 kb on - strand at 67.451 kb on - strand at 67.451 kb on - strand at 67.512 kb on + strand at 67.512 kb on + strand at 67.512 kb on + strand at 67.512 kb on + strand at 67.512 kb on + strand at 67.648 kb on + strand at 67.649 kb on - strand at 67.649 kb on - strand at 67.818 kb on - strand at 68.036 kb on + strand, within GFF4967 at 68.631 kb on + strand at 68.631 kb on + strand at 68.631 kb on + strand at 68.632 kb on - strand at 68.632 kb on - strand at 68.737 kb on - strand, within GFF4968 at 68.737 kb on - strand, within GFF4968 at 68.809 kb on - strand, within GFF4968 at 68.943 kb on + strand, within GFF4968 at 68.944 kb on - strand, within GFF4968 at 68.949 kb on + strand, within GFF4968 at 68.949 kb on + strand, within GFF4968 at 69.078 kb on + strand, within GFF4968 at 69.079 kb on - strand, within GFF4968 at 69.103 kb on - strand, within GFF4968 at 69.354 kb on + strand, within GFF4968
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 67,031 + +0.2 67,031 + +1.3 67,174 + GFF4966 0.38 +0.9 67,174 + GFF4966 0.38 -0.3 67,175 - GFF4966 0.38 -0.5 67,175 - GFF4966 0.38 -0.2 67,175 - GFF4966 0.38 -0.1 67,337 + -0.8 67,338 - -0.4 67,428 + +2.2 67,428 + +0.0 67,428 + -0.5 67,429 - +0.8 67,429 - -0.2 67,429 - -0.2 67,429 - -0.3 67,450 + -0.5 67,451 - -0.4 67,451 - +0.0 67,451 - -0.7 67,512 + -0.2 67,512 + +0.5 67,512 + -0.8 67,512 + +1.5 67,512 + +1.2 67,648 + -0.2 67,649 - -0.7 67,649 - -0.7 67,818 - +0.0 68,036 + GFF4967 0.28 +0.7 68,631 + +0.0 68,631 + +0.3 68,631 + -0.1 68,632 - -0.1 68,632 - -0.5 68,737 - GFF4968 0.14 -0.8 68,737 - GFF4968 0.14 +0.4 68,809 - GFF4968 0.18 +0.9 68,943 + GFF4968 0.26 -0.8 68,944 - GFF4968 0.26 -0.6 68,949 + GFF4968 0.26 -0.5 68,949 + GFF4968 0.26 +0.5 69,078 + GFF4968 0.34 +0.7 69,079 - GFF4968 0.34 -0.3 69,103 - GFF4968 0.35 -1.3 69,354 + GFF4968 0.50 -1.4
Or see this region's nucleotide sequence