Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4776 and GFF4777 overlap by 19 nucleotides GFF4777 and GFF4778 overlap by 4 nucleotides GFF4778 and GFF4779 are separated by 10 nucleotides
GFF4776 - Broad-specificity amino acid ABC transporter, substrate-binding protein, at 144,568 to 145,761
GFF4776
GFF4777 - no description, at 145,743 to 145,901
GFF4777
GFF4778 - Broad-specificity amino acid ABC transporter, permease protein 1, at 145,898 to 146,773
GFF4778
GFF4779 - Broad-specificity amino acid ABC transporter, permease protein 2, at 146,784 to 147,776
GFF4779
Position (kb) 145
146
147 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 144.991 kb on + strand, within GFF4776 at 144.992 kb on - strand, within GFF4776 at 144.997 kb on + strand, within GFF4776 at 145.081 kb on + strand, within GFF4776 at 145.082 kb on - strand, within GFF4776 at 145.173 kb on + strand, within GFF4776 at 145.173 kb on + strand, within GFF4776 at 145.173 kb on + strand, within GFF4776 at 145.483 kb on + strand, within GFF4776 at 145.484 kb on - strand, within GFF4776 at 145.501 kb on + strand, within GFF4776 at 145.501 kb on + strand, within GFF4776 at 145.501 kb on + strand, within GFF4776 at 145.585 kb on + strand, within GFF4776 at 145.586 kb on - strand, within GFF4776 at 145.633 kb on + strand, within GFF4776 at 145.633 kb on + strand, within GFF4776 at 145.633 kb on + strand, within GFF4776 at 145.633 kb on + strand, within GFF4776 at 145.633 kb on + strand, within GFF4776 at 145.634 kb on - strand, within GFF4776 at 145.634 kb on - strand, within GFF4776 at 145.634 kb on - strand, within GFF4776 at 145.634 kb on - strand, within GFF4776 at 145.634 kb on - strand, within GFF4776 at 145.634 kb on - strand, within GFF4776 at 145.634 kb on - strand, within GFF4776 at 145.634 kb on - strand, within GFF4776 at 145.681 kb on + strand at 145.681 kb on + strand at 145.682 kb on - strand at 145.682 kb on - strand at 145.682 kb on - strand at 145.684 kb on + strand at 145.684 kb on + strand at 145.684 kb on + strand at 145.684 kb on + strand at 145.684 kb on + strand at 145.684 kb on + strand at 145.685 kb on - strand at 145.685 kb on - strand at 145.685 kb on - strand at 145.685 kb on - strand at 145.685 kb on - strand at 145.685 kb on - strand at 145.685 kb on - strand at 145.685 kb on - strand at 145.760 kb on - strand at 145.891 kb on + strand at 145.892 kb on - strand at 145.892 kb on - strand at 145.892 kb on - strand at 145.892 kb on - strand at 145.897 kb on + strand at 145.898 kb on - strand at 145.898 kb on - strand at 145.903 kb on + strand at 145.904 kb on - strand at 145.961 kb on + strand at 145.961 kb on + strand at 145.961 kb on + strand at 145.962 kb on - strand at 146.090 kb on + strand, within GFF4778 at 146.090 kb on + strand, within GFF4778 at 146.091 kb on - strand, within GFF4778 at 146.171 kb on + strand, within GFF4778 at 146.172 kb on - strand, within GFF4778 at 146.249 kb on + strand, within GFF4778 at 146.249 kb on + strand, within GFF4778 at 146.250 kb on - strand, within GFF4778 at 146.330 kb on + strand, within GFF4778 at 146.381 kb on + strand, within GFF4778 at 146.381 kb on + strand, within GFF4778 at 146.382 kb on - strand, within GFF4778 at 146.411 kb on + strand, within GFF4778 at 146.411 kb on + strand, within GFF4778 at 146.412 kb on - strand, within GFF4778 at 146.489 kb on + strand, within GFF4778 at 146.490 kb on - strand, within GFF4778 at 146.771 kb on + strand at 146.800 kb on + strand at 146.982 kb on + strand, within GFF4779 at 146.982 kb on + strand, within GFF4779 at 147.108 kb on + strand, within GFF4779 at 147.108 kb on + strand, within GFF4779 at 147.255 kb on + strand, within GFF4779 at 147.255 kb on + strand, within GFF4779 at 147.255 kb on + strand, within GFF4779 at 147.255 kb on + strand, within GFF4779 at 147.255 kb on + strand, within GFF4779 at 147.255 kb on + strand, within GFF4779 at 147.256 kb on - strand, within GFF4779 at 147.256 kb on - strand, within GFF4779 at 147.258 kb on + strand, within GFF4779 at 147.258 kb on + strand, within GFF4779 at 147.258 kb on + strand, within GFF4779 at 147.258 kb on + strand, within GFF4779 at 147.258 kb on + strand, within GFF4779 at 147.258 kb on + strand, within GFF4779 at 147.259 kb on - strand, within GFF4779 at 147.259 kb on - strand, within GFF4779 at 147.259 kb on - strand, within GFF4779 at 147.259 kb on - strand, within GFF4779
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 144,991 + GFF4776 0.35 +0.6 144,992 - GFF4776 0.36 +0.9 144,997 + GFF4776 0.36 +0.1 145,081 + GFF4776 0.43 +0.2 145,082 - GFF4776 0.43 +0.2 145,173 + GFF4776 0.51 +0.4 145,173 + GFF4776 0.51 -0.0 145,173 + GFF4776 0.51 -0.3 145,483 + GFF4776 0.77 -0.1 145,484 - GFF4776 0.77 +0.1 145,501 + GFF4776 0.78 -0.5 145,501 + GFF4776 0.78 -1.0 145,501 + GFF4776 0.78 -0.8 145,585 + GFF4776 0.85 -0.5 145,586 - GFF4776 0.85 -0.4 145,633 + GFF4776 0.89 -0.1 145,633 + GFF4776 0.89 +1.4 145,633 + GFF4776 0.89 +0.6 145,633 + GFF4776 0.89 -0.0 145,633 + GFF4776 0.89 +0.6 145,634 - GFF4776 0.89 -0.2 145,634 - GFF4776 0.89 -0.1 145,634 - GFF4776 0.89 -0.2 145,634 - GFF4776 0.89 +1.7 145,634 - GFF4776 0.89 -1.8 145,634 - GFF4776 0.89 +0.1 145,634 - GFF4776 0.89 +1.9 145,634 - GFF4776 0.89 -0.3 145,681 + -0.1 145,681 + -0.6 145,682 - -0.4 145,682 - +0.4 145,682 - -0.7 145,684 + -0.5 145,684 + +0.0 145,684 + +0.4 145,684 + -0.2 145,684 + +0.1 145,684 + +1.9 145,685 - -1.7 145,685 - -0.7 145,685 - -1.0 145,685 - +0.5 145,685 - -0.2 145,685 - +0.4 145,685 - -0.5 145,685 - -1.0 145,760 - +0.0 145,891 + +1.4 145,892 - -0.3 145,892 - +0.6 145,892 - -0.7 145,892 - -0.2 145,897 + -0.9 145,898 - -0.6 145,898 - +0.1 145,903 + -0.1 145,904 - -1.3 145,961 + +0.3 145,961 + -1.2 145,961 + -1.1 145,962 - +0.1 146,090 + GFF4778 0.22 -1.3 146,090 + GFF4778 0.22 -0.7 146,091 - GFF4778 0.22 -1.3 146,171 + GFF4778 0.31 +0.3 146,172 - GFF4778 0.31 +0.2 146,249 + GFF4778 0.40 +0.4 146,249 + GFF4778 0.40 -0.6 146,250 - GFF4778 0.40 -0.2 146,330 + GFF4778 0.49 +0.4 146,381 + GFF4778 0.55 -1.2 146,381 + GFF4778 0.55 +1.0 146,382 - GFF4778 0.55 -0.8 146,411 + GFF4778 0.59 +0.9 146,411 + GFF4778 0.59 -1.3 146,412 - GFF4778 0.59 +0.2 146,489 + GFF4778 0.67 -1.2 146,490 - GFF4778 0.68 -0.2 146,771 + -1.0 146,800 + -0.2 146,982 + GFF4779 0.20 -0.9 146,982 + GFF4779 0.20 -0.3 147,108 + GFF4779 0.33 -0.3 147,108 + GFF4779 0.33 -0.3 147,255 + GFF4779 0.47 +0.2 147,255 + GFF4779 0.47 -0.1 147,255 + GFF4779 0.47 +0.5 147,255 + GFF4779 0.47 +2.8 147,255 + GFF4779 0.47 -0.6 147,255 + GFF4779 0.47 +1.1 147,256 - GFF4779 0.48 +0.8 147,256 - GFF4779 0.48 -0.0 147,258 + GFF4779 0.48 +0.1 147,258 + GFF4779 0.48 +0.4 147,258 + GFF4779 0.48 +0.4 147,258 + GFF4779 0.48 -2.3 147,258 + GFF4779 0.48 +0.4 147,258 + GFF4779 0.48 -0.8 147,259 - GFF4779 0.48 +3.4 147,259 - GFF4779 0.48 -1.8 147,259 - GFF4779 0.48 +1.2 147,259 - GFF4779 0.48 +0.8
Or see this region's nucleotide sequence