Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4720 and GFF4721 are separated by 150 nucleotides GFF4721 and GFF4722 are separated by 16 nucleotides
GFF4720 - Putrescine transport system permease protein potI, at 81,644 to 82,534
GFF4720
GFF4721 - ABC transporter, permease protein 1 (cluster 1, maltose/g3p/polyamine/iron), at 82,685 to 83,602
GFF4721
GFF4722 - ABC transporter, substrate-binding protein (cluster 1, maltose/g3p/polyamine/iron), at 83,619 to 84,887
GFF4722
Position (kb)
82
83
84 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 81.744 kb on + strand, within GFF4720 at 81.744 kb on + strand, within GFF4720 at 81.745 kb on - strand, within GFF4720 at 81.748 kb on + strand, within GFF4720 at 81.748 kb on + strand, within GFF4720 at 81.749 kb on - strand, within GFF4720 at 81.749 kb on - strand, within GFF4720 at 81.749 kb on - strand, within GFF4720 at 81.749 kb on - strand, within GFF4720 at 81.749 kb on - strand, within GFF4720 at 81.960 kb on + strand, within GFF4720 at 81.961 kb on - strand, within GFF4720 at 82.071 kb on + strand, within GFF4720 at 82.194 kb on - strand, within GFF4720 at 82.452 kb on + strand at 82.520 kb on + strand at 82.520 kb on + strand at 82.521 kb on - strand at 82.521 kb on - strand at 82.613 kb on - strand at 82.613 kb on - strand at 82.791 kb on + strand, within GFF4721 at 82.791 kb on + strand, within GFF4721 at 82.791 kb on + strand, within GFF4721 at 82.791 kb on + strand, within GFF4721 at 82.792 kb on - strand, within GFF4721 at 82.792 kb on - strand, within GFF4721 at 82.792 kb on - strand, within GFF4721 at 82.792 kb on - strand, within GFF4721 at 83.046 kb on + strand, within GFF4721 at 83.047 kb on - strand, within GFF4721 at 83.047 kb on - strand, within GFF4721 at 83.047 kb on - strand, within GFF4721 at 83.274 kb on + strand, within GFF4721 at 83.275 kb on - strand, within GFF4721 at 83.343 kb on + strand, within GFF4721 at 83.343 kb on + strand, within GFF4721 at 83.343 kb on + strand, within GFF4721 at 83.343 kb on + strand, within GFF4721 at 83.343 kb on + strand, within GFF4721 at 83.343 kb on + strand, within GFF4721 at 83.343 kb on + strand, within GFF4721 at 83.344 kb on - strand, within GFF4721 at 83.344 kb on - strand, within GFF4721 at 83.413 kb on - strand, within GFF4721 at 83.413 kb on - strand, within GFF4721 at 83.413 kb on - strand, within GFF4721 at 83.445 kb on + strand, within GFF4721 at 83.445 kb on + strand, within GFF4721 at 83.656 kb on + strand at 83.656 kb on + strand at 83.656 kb on + strand at 83.656 kb on + strand at 83.656 kb on + strand at 83.656 kb on + strand at 83.656 kb on + strand at 83.656 kb on + strand at 83.657 kb on - strand at 83.657 kb on - strand at 83.657 kb on - strand at 83.657 kb on - strand at 83.657 kb on - strand at 83.657 kb on - strand at 83.713 kb on + strand at 83.714 kb on - strand at 83.714 kb on - strand at 83.807 kb on - strand, within GFF4722 at 83.818 kb on + strand, within GFF4722 at 83.818 kb on + strand, within GFF4722 at 83.819 kb on - strand, within GFF4722 at 83.819 kb on - strand, within GFF4722 at 83.860 kb on + strand, within GFF4722 at 83.860 kb on + strand, within GFF4722 at 83.860 kb on + strand, within GFF4722 at 83.860 kb on + strand, within GFF4722 at 83.861 kb on - strand, within GFF4722 at 83.861 kb on - strand, within GFF4722 at 83.861 kb on - strand, within GFF4722 at 83.861 kb on - strand, within GFF4722 at 83.861 kb on - strand, within GFF4722 at 83.861 kb on - strand, within GFF4722 at 83.863 kb on + strand, within GFF4722 at 83.864 kb on - strand, within GFF4722 at 83.881 kb on + strand, within GFF4722 at 83.881 kb on + strand, within GFF4722 at 83.923 kb on + strand, within GFF4722 at 83.923 kb on + strand, within GFF4722 at 83.924 kb on - strand, within GFF4722 at 83.990 kb on - strand, within GFF4722 at 84.215 kb on - strand, within GFF4722 at 84.215 kb on - strand, within GFF4722 at 84.382 kb on + strand, within GFF4722 at 84.382 kb on + strand, within GFF4722 at 84.382 kb on + strand, within GFF4722 at 84.382 kb on + strand, within GFF4722 at 84.382 kb on + strand, within GFF4722 at 84.382 kb on + strand, within GFF4722 at 84.383 kb on - strand, within GFF4722 at 84.383 kb on - strand, within GFF4722 at 84.383 kb on - strand, within GFF4722 at 84.383 kb on - strand, within GFF4722 at 84.383 kb on - strand, within GFF4722 at 84.406 kb on + strand, within GFF4722 at 84.406 kb on + strand, within GFF4722 at 84.406 kb on + strand, within GFF4722 at 84.406 kb on + strand, within GFF4722 at 84.406 kb on + strand, within GFF4722 at 84.406 kb on + strand, within GFF4722 at 84.406 kb on + strand, within GFF4722 at 84.406 kb on + strand, within GFF4722 at 84.407 kb on - strand, within GFF4722 at 84.407 kb on - strand, within GFF4722 at 84.407 kb on - strand, within GFF4722 at 84.407 kb on - strand, within GFF4722 at 84.407 kb on - strand, within GFF4722 at 84.454 kb on + strand, within GFF4722 at 84.455 kb on - strand, within GFF4722
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 81,744 + GFF4720 0.11 -0.3 81,744 + GFF4720 0.11 -0.3 81,745 - GFF4720 0.11 +0.3 81,748 + GFF4720 0.12 +1.6 81,748 + GFF4720 0.12 -0.3 81,749 - GFF4720 0.12 +0.0 81,749 - GFF4720 0.12 +0.2 81,749 - GFF4720 0.12 -0.7 81,749 - GFF4720 0.12 -0.1 81,749 - GFF4720 0.12 +0.0 81,960 + GFF4720 0.35 -1.4 81,961 - GFF4720 0.36 -0.5 82,071 + GFF4720 0.48 -0.6 82,194 - GFF4720 0.62 -0.5 82,452 + -1.0 82,520 + -0.7 82,520 + -0.4 82,521 - -0.6 82,521 - -0.9 82,613 - -0.2 82,613 - -0.4 82,791 + GFF4721 0.12 -1.1 82,791 + GFF4721 0.12 -0.1 82,791 + GFF4721 0.12 -1.2 82,791 + GFF4721 0.12 +0.4 82,792 - GFF4721 0.12 -0.7 82,792 - GFF4721 0.12 +0.2 82,792 - GFF4721 0.12 -1.1 82,792 - GFF4721 0.12 -0.5 83,046 + GFF4721 0.39 +0.9 83,047 - GFF4721 0.39 -0.3 83,047 - GFF4721 0.39 -1.6 83,047 - GFF4721 0.39 -0.1 83,274 + GFF4721 0.64 -0.2 83,275 - GFF4721 0.64 -0.2 83,343 + GFF4721 0.72 -1.0 83,343 + GFF4721 0.72 +0.0 83,343 + GFF4721 0.72 +0.4 83,343 + GFF4721 0.72 +1.4 83,343 + GFF4721 0.72 -0.8 83,343 + GFF4721 0.72 -0.3 83,343 + GFF4721 0.72 -0.6 83,344 - GFF4721 0.72 -0.1 83,344 - GFF4721 0.72 +1.9 83,413 - GFF4721 0.79 -2.6 83,413 - GFF4721 0.79 -0.1 83,413 - GFF4721 0.79 -0.0 83,445 + GFF4721 0.83 -1.7 83,445 + GFF4721 0.83 -2.3 83,656 + +0.9 83,656 + +0.2 83,656 + -0.5 83,656 + +0.3 83,656 + -1.7 83,656 + -0.2 83,656 + -1.1 83,656 + -0.2 83,657 - +0.3 83,657 - -0.3 83,657 - +1.4 83,657 - -0.5 83,657 - -0.4 83,657 - -0.5 83,713 + +3.7 83,714 - -0.0 83,714 - -0.3 83,807 - GFF4722 0.15 -0.5 83,818 + GFF4722 0.16 +0.1 83,818 + GFF4722 0.16 -0.4 83,819 - GFF4722 0.16 +0.1 83,819 - GFF4722 0.16 +1.8 83,860 + GFF4722 0.19 -0.2 83,860 + GFF4722 0.19 +0.3 83,860 + GFF4722 0.19 -0.2 83,860 + GFF4722 0.19 -0.9 83,861 - GFF4722 0.19 +1.1 83,861 - GFF4722 0.19 -0.7 83,861 - GFF4722 0.19 +0.1 83,861 - GFF4722 0.19 +0.1 83,861 - GFF4722 0.19 +0.0 83,861 - GFF4722 0.19 -0.4 83,863 + GFF4722 0.19 -0.7 83,864 - GFF4722 0.19 -0.9 83,881 + GFF4722 0.21 -0.8 83,881 + GFF4722 0.21 +0.1 83,923 + GFF4722 0.24 -2.7 83,923 + GFF4722 0.24 -0.2 83,924 - GFF4722 0.24 +0.3 83,990 - GFF4722 0.29 -0.1 84,215 - GFF4722 0.47 -0.3 84,215 - GFF4722 0.47 -0.2 84,382 + GFF4722 0.60 -0.6 84,382 + GFF4722 0.60 -0.2 84,382 + GFF4722 0.60 +1.7 84,382 + GFF4722 0.60 -0.2 84,382 + GFF4722 0.60 +0.8 84,382 + GFF4722 0.60 -0.0 84,383 - GFF4722 0.60 +0.5 84,383 - GFF4722 0.60 -0.3 84,383 - GFF4722 0.60 -0.9 84,383 - GFF4722 0.60 -0.2 84,383 - GFF4722 0.60 -0.1 84,406 + GFF4722 0.62 -0.6 84,406 + GFF4722 0.62 +0.1 84,406 + GFF4722 0.62 +0.5 84,406 + GFF4722 0.62 -0.1 84,406 + GFF4722 0.62 +0.0 84,406 + GFF4722 0.62 +0.0 84,406 + GFF4722 0.62 +1.7 84,406 + GFF4722 0.62 -1.0 84,407 - GFF4722 0.62 +0.9 84,407 - GFF4722 0.62 -1.1 84,407 - GFF4722 0.62 -1.6 84,407 - GFF4722 0.62 -0.4 84,407 - GFF4722 0.62 +0.2 84,454 + GFF4722 0.66 -0.5 84,455 - GFF4722 0.66 -1.1
Or see this region's nucleotide sequence