Strain Fitness in Variovorax sp. SCN45 around GFF4721

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4720 and GFF4721 are separated by 150 nucleotidesGFF4721 and GFF4722 are separated by 16 nucleotides GFF4720 - Putrescine transport system permease protein potI, at 81,644 to 82,534 GFF4720 GFF4721 - ABC transporter, permease protein 1 (cluster 1, maltose/g3p/polyamine/iron), at 82,685 to 83,602 GFF4721 GFF4722 - ABC transporter, substrate-binding protein (cluster 1, maltose/g3p/polyamine/iron), at 83,619 to 84,887 GFF4722 Position (kb) 82 83 84Strain fitness (log2 ratio) -2 -1 0 1 2 3at 81.744 kb on + strand, within GFF4720at 81.744 kb on + strand, within GFF4720at 81.745 kb on - strand, within GFF4720at 81.748 kb on + strand, within GFF4720at 81.748 kb on + strand, within GFF4720at 81.749 kb on - strand, within GFF4720at 81.749 kb on - strand, within GFF4720at 81.749 kb on - strand, within GFF4720at 81.749 kb on - strand, within GFF4720at 81.749 kb on - strand, within GFF4720at 81.960 kb on + strand, within GFF4720at 81.961 kb on - strand, within GFF4720at 82.071 kb on + strand, within GFF4720at 82.194 kb on - strand, within GFF4720at 82.452 kb on + strandat 82.520 kb on + strandat 82.520 kb on + strandat 82.521 kb on - strandat 82.521 kb on - strandat 82.613 kb on - strandat 82.613 kb on - strandat 82.791 kb on + strand, within GFF4721at 82.791 kb on + strand, within GFF4721at 82.791 kb on + strand, within GFF4721at 82.791 kb on + strand, within GFF4721at 82.792 kb on - strand, within GFF4721at 82.792 kb on - strand, within GFF4721at 82.792 kb on - strand, within GFF4721at 82.792 kb on - strand, within GFF4721at 83.046 kb on + strand, within GFF4721at 83.047 kb on - strand, within GFF4721at 83.047 kb on - strand, within GFF4721at 83.047 kb on - strand, within GFF4721at 83.274 kb on + strand, within GFF4721at 83.275 kb on - strand, within GFF4721at 83.343 kb on + strand, within GFF4721at 83.343 kb on + strand, within GFF4721at 83.343 kb on + strand, within GFF4721at 83.343 kb on + strand, within GFF4721at 83.343 kb on + strand, within GFF4721at 83.343 kb on + strand, within GFF4721at 83.343 kb on + strand, within GFF4721at 83.344 kb on - strand, within GFF4721at 83.344 kb on - strand, within GFF4721at 83.413 kb on - strand, within GFF4721at 83.413 kb on - strand, within GFF4721at 83.413 kb on - strand, within GFF4721at 83.445 kb on + strand, within GFF4721at 83.445 kb on + strand, within GFF4721at 83.656 kb on + strandat 83.656 kb on + strandat 83.656 kb on + strandat 83.656 kb on + strandat 83.656 kb on + strandat 83.656 kb on + strandat 83.656 kb on + strandat 83.656 kb on + strandat 83.657 kb on - strandat 83.657 kb on - strandat 83.657 kb on - strandat 83.657 kb on - strandat 83.657 kb on - strandat 83.657 kb on - strandat 83.713 kb on + strandat 83.714 kb on - strandat 83.714 kb on - strandat 83.807 kb on - strand, within GFF4722at 83.818 kb on + strand, within GFF4722at 83.818 kb on + strand, within GFF4722at 83.819 kb on - strand, within GFF4722at 83.819 kb on - strand, within GFF4722at 83.860 kb on + strand, within GFF4722at 83.860 kb on + strand, within GFF4722at 83.860 kb on + strand, within GFF4722at 83.860 kb on + strand, within GFF4722at 83.861 kb on - strand, within GFF4722at 83.861 kb on - strand, within GFF4722at 83.861 kb on - strand, within GFF4722at 83.861 kb on - strand, within GFF4722at 83.861 kb on - strand, within GFF4722at 83.861 kb on - strand, within GFF4722at 83.863 kb on + strand, within GFF4722at 83.864 kb on - strand, within GFF4722at 83.881 kb on + strand, within GFF4722at 83.881 kb on + strand, within GFF4722at 83.923 kb on + strand, within GFF4722at 83.923 kb on + strand, within GFF4722at 83.924 kb on - strand, within GFF4722at 83.990 kb on - strand, within GFF4722at 84.215 kb on - strand, within GFF4722at 84.215 kb on - strand, within GFF4722at 84.382 kb on + strand, within GFF4722at 84.382 kb on + strand, within GFF4722at 84.382 kb on + strand, within GFF4722at 84.382 kb on + strand, within GFF4722at 84.382 kb on + strand, within GFF4722at 84.382 kb on + strand, within GFF4722at 84.383 kb on - strand, within GFF4722at 84.383 kb on - strand, within GFF4722at 84.383 kb on - strand, within GFF4722at 84.383 kb on - strand, within GFF4722at 84.383 kb on - strand, within GFF4722at 84.406 kb on + strand, within GFF4722at 84.406 kb on + strand, within GFF4722at 84.406 kb on + strand, within GFF4722at 84.406 kb on + strand, within GFF4722at 84.406 kb on + strand, within GFF4722at 84.406 kb on + strand, within GFF4722at 84.406 kb on + strand, within GFF4722at 84.406 kb on + strand, within GFF4722at 84.407 kb on - strand, within GFF4722at 84.407 kb on - strand, within GFF4722at 84.407 kb on - strand, within GFF4722at 84.407 kb on - strand, within GFF4722at 84.407 kb on - strand, within GFF4722at 84.454 kb on + strand, within GFF4722at 84.455 kb on - strand, within GFF4722

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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81,744 + GFF4720 0.11 -0.3
81,744 + GFF4720 0.11 -0.3
81,745 - GFF4720 0.11 +0.3
81,748 + GFF4720 0.12 +1.6
81,748 + GFF4720 0.12 -0.3
81,749 - GFF4720 0.12 +0.0
81,749 - GFF4720 0.12 +0.2
81,749 - GFF4720 0.12 -0.7
81,749 - GFF4720 0.12 -0.1
81,749 - GFF4720 0.12 +0.0
81,960 + GFF4720 0.35 -1.4
81,961 - GFF4720 0.36 -0.5
82,071 + GFF4720 0.48 -0.6
82,194 - GFF4720 0.62 -0.5
82,452 + -1.0
82,520 + -0.7
82,520 + -0.4
82,521 - -0.6
82,521 - -0.9
82,613 - -0.2
82,613 - -0.4
82,791 + GFF4721 0.12 -1.1
82,791 + GFF4721 0.12 -0.1
82,791 + GFF4721 0.12 -1.2
82,791 + GFF4721 0.12 +0.4
82,792 - GFF4721 0.12 -0.7
82,792 - GFF4721 0.12 +0.2
82,792 - GFF4721 0.12 -1.1
82,792 - GFF4721 0.12 -0.5
83,046 + GFF4721 0.39 +0.9
83,047 - GFF4721 0.39 -0.3
83,047 - GFF4721 0.39 -1.6
83,047 - GFF4721 0.39 -0.1
83,274 + GFF4721 0.64 -0.2
83,275 - GFF4721 0.64 -0.2
83,343 + GFF4721 0.72 -1.0
83,343 + GFF4721 0.72 +0.0
83,343 + GFF4721 0.72 +0.4
83,343 + GFF4721 0.72 +1.4
83,343 + GFF4721 0.72 -0.8
83,343 + GFF4721 0.72 -0.3
83,343 + GFF4721 0.72 -0.6
83,344 - GFF4721 0.72 -0.1
83,344 - GFF4721 0.72 +1.9
83,413 - GFF4721 0.79 -2.6
83,413 - GFF4721 0.79 -0.1
83,413 - GFF4721 0.79 -0.0
83,445 + GFF4721 0.83 -1.7
83,445 + GFF4721 0.83 -2.3
83,656 + +0.9
83,656 + +0.2
83,656 + -0.5
83,656 + +0.3
83,656 + -1.7
83,656 + -0.2
83,656 + -1.1
83,656 + -0.2
83,657 - +0.3
83,657 - -0.3
83,657 - +1.4
83,657 - -0.5
83,657 - -0.4
83,657 - -0.5
83,713 + +3.7
83,714 - -0.0
83,714 - -0.3
83,807 - GFF4722 0.15 -0.5
83,818 + GFF4722 0.16 +0.1
83,818 + GFF4722 0.16 -0.4
83,819 - GFF4722 0.16 +0.1
83,819 - GFF4722 0.16 +1.8
83,860 + GFF4722 0.19 -0.2
83,860 + GFF4722 0.19 +0.3
83,860 + GFF4722 0.19 -0.2
83,860 + GFF4722 0.19 -0.9
83,861 - GFF4722 0.19 +1.1
83,861 - GFF4722 0.19 -0.7
83,861 - GFF4722 0.19 +0.1
83,861 - GFF4722 0.19 +0.1
83,861 - GFF4722 0.19 +0.0
83,861 - GFF4722 0.19 -0.4
83,863 + GFF4722 0.19 -0.7
83,864 - GFF4722 0.19 -0.9
83,881 + GFF4722 0.21 -0.8
83,881 + GFF4722 0.21 +0.1
83,923 + GFF4722 0.24 -2.7
83,923 + GFF4722 0.24 -0.2
83,924 - GFF4722 0.24 +0.3
83,990 - GFF4722 0.29 -0.1
84,215 - GFF4722 0.47 -0.3
84,215 - GFF4722 0.47 -0.2
84,382 + GFF4722 0.60 -0.6
84,382 + GFF4722 0.60 -0.2
84,382 + GFF4722 0.60 +1.7
84,382 + GFF4722 0.60 -0.2
84,382 + GFF4722 0.60 +0.8
84,382 + GFF4722 0.60 -0.0
84,383 - GFF4722 0.60 +0.5
84,383 - GFF4722 0.60 -0.3
84,383 - GFF4722 0.60 -0.9
84,383 - GFF4722 0.60 -0.2
84,383 - GFF4722 0.60 -0.1
84,406 + GFF4722 0.62 -0.6
84,406 + GFF4722 0.62 +0.1
84,406 + GFF4722 0.62 +0.5
84,406 + GFF4722 0.62 -0.1
84,406 + GFF4722 0.62 +0.0
84,406 + GFF4722 0.62 +0.0
84,406 + GFF4722 0.62 +1.7
84,406 + GFF4722 0.62 -1.0
84,407 - GFF4722 0.62 +0.9
84,407 - GFF4722 0.62 -1.1
84,407 - GFF4722 0.62 -1.6
84,407 - GFF4722 0.62 -0.4
84,407 - GFF4722 0.62 +0.2
84,454 + GFF4722 0.66 -0.5
84,455 - GFF4722 0.66 -1.1

Or see this region's nucleotide sequence