Strain Fitness in Variovorax sp. SCN45 around GFF444

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF442 and GFF443 overlap by 1 nucleotidesGFF443 and GFF444 are separated by 54 nucleotidesGFF444 and GFF445 are separated by 22 nucleotidesGFF445 and GFF446 overlap by 4 nucleotides GFF442 - hypothetical transporter PduT for various metalloporphyrins, at 481,543 to 482,016 GFF442 GFF443 - Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family, at 482,016 to 482,705 GFF443 GFF444 - Cob(I)alamin adenosyltransferase (EC 2.5.1.17) @ Cob(I)alamin adenosyltransferase (EC 2.5.1.17), clustered with cobalamin synthesis, at 482,760 to 483,329 GFF444 GFF445 - Ferrichrome transport ATP-binding protein FhuC, at 483,352 to 484,128 GFF445 GFF446 - Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1), at 484,125 to 485,150 GFF446 Position (kb) 482 483 484Strain fitness (log2 ratio) -2 -1 0 1 2at 481.895 kb on + strand, within GFF442at 481.895 kb on + strand, within GFF442at 481.895 kb on + strand, within GFF442at 481.895 kb on + strand, within GFF442at 481.895 kb on + strand, within GFF442at 481.895 kb on + strand, within GFF442at 481.896 kb on - strand, within GFF442at 481.896 kb on - strand, within GFF442at 481.896 kb on - strand, within GFF442at 481.896 kb on - strand, within GFF442at 481.922 kb on + strand, within GFF442at 481.922 kb on + strand, within GFF442at 481.923 kb on - strand, within GFF442at 481.923 kb on - strand, within GFF442at 481.923 kb on - strand, within GFF442at 481.923 kb on - strand, within GFF442at 481.923 kb on - strand, within GFF442at 481.923 kb on - strand, within GFF442at 481.923 kb on - strand, within GFF442at 481.923 kb on - strand, within GFF442at 482.146 kb on + strand, within GFF443at 482.147 kb on - strand, within GFF443at 482.662 kb on + strandat 482.790 kb on - strandat 482.790 kb on - strandat 482.790 kb on - strandat 482.835 kb on - strand, within GFF444at 482.946 kb on + strand, within GFF444at 482.947 kb on + strand, within GFF444at 482.947 kb on + strand, within GFF444at 482.947 kb on + strand, within GFF444at 482.948 kb on - strand, within GFF444at 482.948 kb on - strand, within GFF444at 482.948 kb on - strand, within GFF444at 482.948 kb on - strand, within GFF444at 482.959 kb on + strand, within GFF444at 482.960 kb on - strand, within GFF444at 482.960 kb on - strand, within GFF444at 483.169 kb on + strand, within GFF444at 483.169 kb on + strand, within GFF444at 483.169 kb on + strand, within GFF444at 483.169 kb on + strand, within GFF444at 483.170 kb on - strand, within GFF444at 483.170 kb on - strand, within GFF444at 483.295 kb on + strandat 483.296 kb on - strandat 483.296 kb on - strandat 483.312 kb on - strandat 483.344 kb on + strandat 483.344 kb on + strandat 483.344 kb on + strandat 483.344 kb on + strandat 483.344 kb on + strandat 483.344 kb on + strandat 483.344 kb on + strandat 483.344 kb on + strandat 483.344 kb on + strandat 483.345 kb on - strandat 483.345 kb on - strandat 483.345 kb on - strandat 483.345 kb on - strandat 483.345 kb on - strandat 483.345 kb on - strandat 483.345 kb on - strandat 483.345 kb on - strandat 483.345 kb on - strandat 483.345 kb on - strandat 483.345 kb on - strandat 483.345 kb on - strandat 483.345 kb on - strandat 483.345 kb on - strandat 483.353 kb on + strandat 483.830 kb on + strand, within GFF445at 483.830 kb on + strand, within GFF445at 483.830 kb on + strand, within GFF445at 483.830 kb on + strand, within GFF445at 483.830 kb on + strand, within GFF445at 483.831 kb on - strand, within GFF445at 483.831 kb on - strand, within GFF445at 483.831 kb on - strand, within GFF445at 483.831 kb on - strand, within GFF445at 483.831 kb on - strand, within GFF445at 483.831 kb on - strand, within GFF445at 483.831 kb on - strand, within GFF445at 484.174 kb on + strandat 484.174 kb on + strandat 484.175 kb on - strandat 484.175 kb on - strandat 484.175 kb on - strandat 484.175 kb on - strandat 484.175 kb on - strandat 484.175 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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481,895 + GFF442 0.74 -0.3
481,895 + GFF442 0.74 -1.6
481,895 + GFF442 0.74 -0.5
481,895 + GFF442 0.74 -0.5
481,895 + GFF442 0.74 -0.9
481,895 + GFF442 0.74 -1.0
481,896 - GFF442 0.74 -0.9
481,896 - GFF442 0.74 -0.2
481,896 - GFF442 0.74 +0.5
481,896 - GFF442 0.74 -0.2
481,922 + GFF442 0.80 -1.1
481,922 + GFF442 0.80 -0.5
481,923 - GFF442 0.80 +0.2
481,923 - GFF442 0.80 +0.9
481,923 - GFF442 0.80 +1.2
481,923 - GFF442 0.80 +0.7
481,923 - GFF442 0.80 +0.2
481,923 - GFF442 0.80 +1.2
481,923 - GFF442 0.80 +0.5
481,923 - GFF442 0.80 -0.3
482,146 + GFF443 0.19 +2.3
482,147 - GFF443 0.19 -0.4
482,662 + -0.3
482,790 - -0.1
482,790 - +0.5
482,790 - +0.5
482,835 - GFF444 0.13 +1.3
482,946 + GFF444 0.33 -1.3
482,947 + GFF444 0.33 +0.3
482,947 + GFF444 0.33 +0.3
482,947 + GFF444 0.33 -0.1
482,948 - GFF444 0.33 +0.0
482,948 - GFF444 0.33 +0.5
482,948 - GFF444 0.33 -1.3
482,948 - GFF444 0.33 +0.1
482,959 + GFF444 0.35 -0.6
482,960 - GFF444 0.35 +0.0
482,960 - GFF444 0.35 -0.4
483,169 + GFF444 0.72 +1.3
483,169 + GFF444 0.72 +0.8
483,169 + GFF444 0.72 -1.3
483,169 + GFF444 0.72 +0.5
483,170 - GFF444 0.72 -0.4
483,170 - GFF444 0.72 +0.1
483,295 + +0.3
483,296 - +0.4
483,296 - -1.7
483,312 - -0.7
483,344 + -0.4
483,344 + -0.6
483,344 + -0.2
483,344 + +0.1
483,344 + +1.7
483,344 + +0.3
483,344 + -1.1
483,344 + -0.2
483,344 + +0.3
483,345 - +0.4
483,345 - -0.6
483,345 - -1.6
483,345 - +0.4
483,345 - -0.3
483,345 - -1.6
483,345 - +0.5
483,345 - -1.6
483,345 - -0.4
483,345 - +0.7
483,345 - -2.0
483,345 - +0.4
483,345 - -1.4
483,345 - -0.3
483,353 + -0.4
483,830 + GFF445 0.62 -0.0
483,830 + GFF445 0.62 -0.3
483,830 + GFF445 0.62 -2.1
483,830 + GFF445 0.62 -0.5
483,830 + GFF445 0.62 -0.5
483,831 - GFF445 0.62 -0.1
483,831 - GFF445 0.62 -0.3
483,831 - GFF445 0.62 -0.3
483,831 - GFF445 0.62 +0.1
483,831 - GFF445 0.62 -1.1
483,831 - GFF445 0.62 +1.1
483,831 - GFF445 0.62 -1.3
484,174 + -0.3
484,174 + +0.1
484,175 - -0.5
484,175 - +0.0
484,175 - -0.7
484,175 - +0.4
484,175 - -0.1
484,175 - -0.3

Or see this region's nucleotide sequence