Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF432 and GFF433 are separated by 220 nucleotides GFF433 and GFF434 are separated by 8 nucleotides GFF434 and GFF435 are separated by 25 nucleotides GFF435 and GFF436 are separated by 46 nucleotides
GFF432 - Probable transcription regulator protein of MDR efflux pump cluster, at 470,876 to 471,790
GFF432
GFF433 - Flagellar brake protein YcgR, at 472,011 to 472,766
GFF433
GFF434 - OsmC/Ohr family protein, at 472,775 to 473,239
GFF434
GFF435 - hypothetical protein, at 473,265 to 473,816
GFF435
GFF436 - LrgA-associated membrane protein LrgB, at 473,863 to 474,594
GFF436
Position (kb) 472
473
474 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 471.834 kb on + strand at 471.834 kb on + strand at 471.834 kb on + strand at 471.835 kb on - strand at 471.835 kb on - strand at 471.835 kb on - strand at 471.835 kb on - strand at 471.835 kb on - strand at 471.835 kb on - strand at 471.851 kb on + strand at 471.851 kb on + strand at 471.851 kb on + strand at 471.875 kb on + strand at 471.876 kb on - strand at 471.883 kb on + strand at 471.883 kb on + strand at 471.883 kb on + strand at 471.883 kb on + strand at 471.884 kb on - strand at 471.884 kb on - strand at 471.884 kb on - strand at 471.884 kb on - strand at 471.884 kb on - strand at 471.884 kb on - strand at 471.884 kb on - strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.996 kb on - strand at 472.016 kb on + strand at 472.016 kb on + strand at 472.016 kb on + strand at 472.017 kb on - strand at 472.017 kb on - strand at 472.017 kb on - strand at 472.146 kb on + strand, within GFF433 at 472.146 kb on + strand, within GFF433 at 472.146 kb on + strand, within GFF433 at 472.146 kb on + strand, within GFF433 at 472.146 kb on + strand, within GFF433 at 472.146 kb on + strand, within GFF433 at 472.146 kb on + strand, within GFF433 at 472.147 kb on - strand, within GFF433 at 472.147 kb on - strand, within GFF433 at 472.380 kb on + strand, within GFF433 at 472.380 kb on + strand, within GFF433 at 472.380 kb on + strand, within GFF433 at 472.381 kb on - strand, within GFF433 at 472.381 kb on - strand, within GFF433 at 472.381 kb on - strand, within GFF433 at 472.381 kb on - strand, within GFF433 at 472.381 kb on - strand, within GFF433 at 472.381 kb on - strand, within GFF433 at 472.381 kb on - strand, within GFF433 at 472.399 kb on - strand, within GFF433 at 472.399 kb on - strand, within GFF433 at 472.657 kb on + strand, within GFF433 at 472.657 kb on + strand, within GFF433 at 472.657 kb on + strand, within GFF433 at 472.657 kb on + strand, within GFF433 at 472.657 kb on + strand, within GFF433 at 472.657 kb on + strand, within GFF433 at 472.657 kb on + strand, within GFF433 at 472.657 kb on + strand, within GFF433 at 472.658 kb on - strand, within GFF433 at 472.658 kb on - strand, within GFF433 at 472.658 kb on - strand, within GFF433 at 472.658 kb on - strand, within GFF433 at 472.658 kb on - strand, within GFF433 at 472.658 kb on - strand, within GFF433 at 472.663 kb on - strand, within GFF433 at 472.663 kb on - strand, within GFF433 at 472.663 kb on - strand, within GFF433 at 472.779 kb on + strand at 472.779 kb on + strand at 472.779 kb on + strand at 472.779 kb on + strand at 472.779 kb on + strand at 472.779 kb on + strand at 472.779 kb on + strand at 472.779 kb on + strand at 472.780 kb on - strand at 472.780 kb on - strand at 472.780 kb on - strand at 472.780 kb on - strand at 472.780 kb on - strand at 472.980 kb on + strand, within GFF434 at 472.980 kb on + strand, within GFF434 at 473.175 kb on + strand, within GFF434 at 473.175 kb on + strand, within GFF434 at 473.175 kb on + strand, within GFF434 at 473.175 kb on + strand, within GFF434 at 473.175 kb on + strand, within GFF434 at 473.175 kb on + strand, within GFF434 at 473.175 kb on + strand, within GFF434 at 473.175 kb on + strand, within GFF434 at 473.175 kb on + strand, within GFF434 at 473.175 kb on + strand, within GFF434 at 473.175 kb on + strand, within GFF434 at 473.176 kb on - strand, within GFF434 at 473.176 kb on - strand, within GFF434 at 473.176 kb on - strand, within GFF434 at 473.176 kb on - strand, within GFF434 at 473.176 kb on - strand, within GFF434 at 473.229 kb on + strand at 473.229 kb on + strand at 473.230 kb on - strand at 473.230 kb on - strand at 473.230 kb on - strand at 473.230 kb on - strand at 473.257 kb on - strand at 473.264 kb on - strand at 473.272 kb on + strand at 473.272 kb on + strand at 473.272 kb on + strand at 473.272 kb on + strand at 473.273 kb on - strand at 473.273 kb on - strand at 473.273 kb on - strand at 473.273 kb on - strand at 473.273 kb on - strand at 473.273 kb on - strand at 473.273 kb on - strand at 473.273 kb on - strand at 473.461 kb on + strand, within GFF435 at 473.494 kb on + strand, within GFF435 at 473.494 kb on + strand, within GFF435 at 473.495 kb on - strand, within GFF435 at 473.515 kb on + strand, within GFF435 at 473.515 kb on + strand, within GFF435 at 473.515 kb on + strand, within GFF435 at 473.516 kb on - strand, within GFF435 at 473.516 kb on - strand, within GFF435 at 473.596 kb on + strand, within GFF435 at 473.597 kb on - strand, within GFF435 at 473.597 kb on - strand, within GFF435 at 473.776 kb on + strand at 473.776 kb on + strand at 473.776 kb on + strand at 473.776 kb on + strand at 473.776 kb on + strand at 473.776 kb on + strand at 473.776 kb on + strand at 473.776 kb on + strand at 473.777 kb on - strand at 473.777 kb on - strand at 473.777 kb on - strand at 473.777 kb on - strand at 473.777 kb on - strand at 473.777 kb on - strand at 473.788 kb on + strand at 473.789 kb on - strand at 473.789 kb on - strand at 473.789 kb on - strand at 473.789 kb on - strand at 473.789 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 471,834 + -0.8 471,834 + -0.7 471,834 + +0.4 471,835 - +0.0 471,835 - +0.3 471,835 - +1.2 471,835 - +0.1 471,835 - -0.4 471,835 - -0.3 471,851 + -0.0 471,851 + +0.5 471,851 + +0.0 471,875 + -0.4 471,876 - -1.9 471,883 + -0.1 471,883 + -1.2 471,883 + +0.0 471,883 + -1.0 471,884 - +0.4 471,884 - -0.1 471,884 - -0.1 471,884 - -1.1 471,884 - -1.6 471,884 - +0.2 471,884 - -2.8 471,994 + -0.4 471,994 + -0.3 471,994 + +0.2 471,994 + +0.2 471,994 + +0.2 471,994 + -0.9 471,994 + -0.6 471,994 + -2.0 471,994 + +0.3 471,994 + +0.4 471,994 + +0.1 471,994 + -0.5 471,994 + -0.9 471,994 + -0.1 471,994 + +0.4 471,994 + -0.5 471,994 + -0.7 471,994 + -0.9 471,994 + -0.2 471,994 + -2.1 471,994 + +0.3 471,994 + -1.6 471,995 - +0.2 471,995 - -1.3 471,995 - -0.6 471,995 - +0.7 471,995 - -0.8 471,995 - +0.5 471,995 - -1.2 471,995 - -0.2 471,995 - -0.0 471,995 - -0.6 471,995 - +0.7 471,995 - -0.6 471,995 - +0.1 471,995 - -0.6 471,995 - -0.2 471,995 - -0.6 471,995 - +0.9 471,995 - +1.2 471,995 - -0.3 471,995 - +0.2 471,995 - -0.5 471,995 - -0.2 471,995 - -0.2 471,995 - -0.7 471,995 - -2.3 471,995 - -1.3 471,995 - -2.1 471,996 - -0.2 472,016 + -1.3 472,016 + -0.5 472,016 + +0.1 472,017 - -2.2 472,017 - +0.6 472,017 - +0.6 472,146 + GFF433 0.18 -0.6 472,146 + GFF433 0.18 +0.5 472,146 + GFF433 0.18 +0.7 472,146 + GFF433 0.18 -0.2 472,146 + GFF433 0.18 -0.3 472,146 + GFF433 0.18 -0.3 472,146 + GFF433 0.18 +0.2 472,147 - GFF433 0.18 -0.0 472,147 - GFF433 0.18 +0.2 472,380 + GFF433 0.49 -0.4 472,380 + GFF433 0.49 +0.0 472,380 + GFF433 0.49 +0.4 472,381 - GFF433 0.49 +0.5 472,381 - GFF433 0.49 +0.6 472,381 - GFF433 0.49 -1.1 472,381 - GFF433 0.49 -0.6 472,381 - GFF433 0.49 +0.4 472,381 - GFF433 0.49 +1.0 472,381 - GFF433 0.49 -0.2 472,399 - GFF433 0.51 -0.9 472,399 - GFF433 0.51 -0.4 472,657 + GFF433 0.85 +0.6 472,657 + GFF433 0.85 -0.6 472,657 + GFF433 0.85 +0.8 472,657 + GFF433 0.85 -0.3 472,657 + GFF433 0.85 -0.5 472,657 + GFF433 0.85 -0.3 472,657 + GFF433 0.85 +0.0 472,657 + GFF433 0.85 -0.6 472,658 - GFF433 0.86 -0.1 472,658 - GFF433 0.86 +0.0 472,658 - GFF433 0.86 -0.1 472,658 - GFF433 0.86 -0.3 472,658 - GFF433 0.86 +1.1 472,658 - GFF433 0.86 -0.7 472,663 - GFF433 0.86 -0.0 472,663 - GFF433 0.86 -0.6 472,663 - GFF433 0.86 -1.7 472,779 + -0.3 472,779 + -0.3 472,779 + +0.4 472,779 + -0.2 472,779 + +0.3 472,779 + -0.8 472,779 + +0.4 472,779 + -0.3 472,780 - -0.6 472,780 - -0.2 472,780 - +0.8 472,780 - +0.0 472,780 - +0.0 472,980 + GFF434 0.44 -0.4 472,980 + GFF434 0.44 +1.2 473,175 + GFF434 0.86 +0.6 473,175 + GFF434 0.86 -0.6 473,175 + GFF434 0.86 -0.8 473,175 + GFF434 0.86 -0.6 473,175 + GFF434 0.86 +0.8 473,175 + GFF434 0.86 -0.3 473,175 + GFF434 0.86 +0.7 473,175 + GFF434 0.86 +1.2 473,175 + GFF434 0.86 -0.2 473,175 + GFF434 0.86 +0.1 473,175 + GFF434 0.86 -0.3 473,176 - GFF434 0.86 -0.2 473,176 - GFF434 0.86 +0.4 473,176 - GFF434 0.86 -1.3 473,176 - GFF434 0.86 +0.1 473,176 - GFF434 0.86 -0.3 473,229 + +1.2 473,229 + +0.2 473,230 - -0.5 473,230 - -0.1 473,230 - -1.0 473,230 - -0.3 473,257 - +0.1 473,264 - -0.2 473,272 + +0.5 473,272 + -1.4 473,272 + +0.1 473,272 + -0.9 473,273 - -0.3 473,273 - -1.0 473,273 - -0.0 473,273 - +0.1 473,273 - -1.9 473,273 - -1.1 473,273 - +0.3 473,273 - -0.8 473,461 + GFF435 0.36 +0.5 473,494 + GFF435 0.41 -0.2 473,494 + GFF435 0.41 -1.2 473,495 - GFF435 0.42 -0.1 473,515 + GFF435 0.45 +0.7 473,515 + GFF435 0.45 -0.8 473,515 + GFF435 0.45 -0.6 473,516 - GFF435 0.45 +1.8 473,516 - GFF435 0.45 -0.7 473,596 + GFF435 0.60 -0.1 473,597 - GFF435 0.60 -1.0 473,597 - GFF435 0.60 +1.0 473,776 + -0.4 473,776 + -0.6 473,776 + -0.2 473,776 + +0.4 473,776 + +0.1 473,776 + -1.3 473,776 + +0.6 473,776 + -0.8 473,777 - +0.4 473,777 - -0.9 473,777 - -2.1 473,777 - +0.2 473,777 - -0.3 473,777 - +1.1 473,788 + -0.1 473,789 - +0.0 473,789 - -0.1 473,789 - +0.9 473,789 - -1.3 473,789 - +0.9
Or see this region's nucleotide sequence