Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4175 and GFF4176 are separated by 90 nucleotides GFF4176 and GFF4177 overlap by 4 nucleotides GFF4177 and GFF4178 are separated by 11 nucleotides GFF4178 and GFF4179 are separated by 57 nucleotides
GFF4175 - FMN reductase (NADH) (EC 1.5.1.42), at 80,356 to 80,949
GFF4175
GFF4176 - Transcriptional activator MetR, at 81,040 to 81,939
GFF4176
GFF4177 - Epoxyqueuosine reductase (EC 1.17.99.6) QueH, at 81,936 to 82,160
GFF4177
GFF4178 - 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20), at 82,172 to 83,029
GFF4178
GFF4179 - hypothetical protein, at 83,087 to 83,692
GFF4179
Position (kb) 81
82
83 Strain fitness (log2 ratio) -2
-1
0
1
2 at 80.996 kb on + strand at 81.230 kb on - strand, within GFF4176 at 81.349 kb on + strand, within GFF4176 at 81.349 kb on + strand, within GFF4176 at 81.529 kb on + strand, within GFF4176 at 81.529 kb on + strand, within GFF4176 at 81.530 kb on - strand, within GFF4176 at 81.581 kb on - strand, within GFF4176 at 81.623 kb on - strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.652 kb on + strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.653 kb on - strand, within GFF4176 at 81.752 kb on + strand, within GFF4176 at 81.784 kb on + strand, within GFF4176 at 81.784 kb on + strand, within GFF4176 at 81.883 kb on + strand at 81.883 kb on + strand at 81.884 kb on - strand at 81.966 kb on + strand, within GFF4177 at 81.966 kb on + strand, within GFF4177 at 81.978 kb on + strand, within GFF4177 at 81.978 kb on + strand, within GFF4177 at 81.978 kb on + strand, within GFF4177 at 81.979 kb on - strand, within GFF4177 at 81.979 kb on - strand, within GFF4177 at 81.979 kb on - strand, within GFF4177 at 82.065 kb on + strand, within GFF4177 at 82.066 kb on - strand, within GFF4177 at 82.066 kb on - strand, within GFF4177 at 82.206 kb on - strand at 82.284 kb on - strand, within GFF4178 at 82.292 kb on + strand, within GFF4178 at 82.292 kb on + strand, within GFF4178 at 82.701 kb on + strand, within GFF4178 at 82.701 kb on + strand, within GFF4178 at 82.701 kb on + strand, within GFF4178 at 82.701 kb on + strand, within GFF4178 at 82.702 kb on - strand, within GFF4178 at 82.702 kb on - strand, within GFF4178 at 82.785 kb on + strand, within GFF4178 at 82.786 kb on - strand, within GFF4178 at 82.796 kb on + strand, within GFF4178 at 82.844 kb on + strand, within GFF4178 at 82.989 kb on + strand at 82.989 kb on + strand at 83.089 kb on - strand at 83.089 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 80,996 + -0.4 81,230 - GFF4176 0.21 +2.2 81,349 + GFF4176 0.34 -2.2 81,349 + GFF4176 0.34 +1.6 81,529 + GFF4176 0.54 -0.6 81,529 + GFF4176 0.54 -1.0 81,530 - GFF4176 0.54 -0.4 81,581 - GFF4176 0.60 +0.5 81,623 - GFF4176 0.65 -0.9 81,652 + GFF4176 0.68 -2.5 81,652 + GFF4176 0.68 -0.5 81,652 + GFF4176 0.68 +2.0 81,652 + GFF4176 0.68 +0.5 81,652 + GFF4176 0.68 -0.3 81,652 + GFF4176 0.68 +0.5 81,652 + GFF4176 0.68 -0.2 81,652 + GFF4176 0.68 -0.6 81,652 + GFF4176 0.68 +0.8 81,652 + GFF4176 0.68 +0.6 81,652 + GFF4176 0.68 +1.0 81,652 + GFF4176 0.68 +0.1 81,652 + GFF4176 0.68 -0.2 81,652 + GFF4176 0.68 -0.2 81,653 - GFF4176 0.68 -0.3 81,653 - GFF4176 0.68 -0.6 81,653 - GFF4176 0.68 -0.8 81,653 - GFF4176 0.68 -0.1 81,653 - GFF4176 0.68 +0.2 81,653 - GFF4176 0.68 -0.4 81,653 - GFF4176 0.68 -0.2 81,653 - GFF4176 0.68 -0.7 81,653 - GFF4176 0.68 +0.8 81,653 - GFF4176 0.68 -0.8 81,653 - GFF4176 0.68 -0.9 81,653 - GFF4176 0.68 -0.0 81,653 - GFF4176 0.68 +0.0 81,653 - GFF4176 0.68 -1.0 81,752 + GFF4176 0.79 -2.2 81,784 + GFF4176 0.83 -0.9 81,784 + GFF4176 0.83 -0.1 81,883 + -0.3 81,883 + -0.1 81,884 - +0.1 81,966 + GFF4177 0.13 +0.5 81,966 + GFF4177 0.13 +0.5 81,978 + GFF4177 0.19 -0.4 81,978 + GFF4177 0.19 +0.3 81,978 + GFF4177 0.19 -0.2 81,979 - GFF4177 0.19 -0.9 81,979 - GFF4177 0.19 -0.7 81,979 - GFF4177 0.19 +0.1 82,065 + GFF4177 0.57 +1.9 82,066 - GFF4177 0.58 +1.5 82,066 - GFF4177 0.58 -0.2 82,206 - +0.6 82,284 - GFF4178 0.13 -1.9 82,292 + GFF4178 0.14 +0.5 82,292 + GFF4178 0.14 -0.3 82,701 + GFF4178 0.62 +0.2 82,701 + GFF4178 0.62 -0.2 82,701 + GFF4178 0.62 -0.5 82,701 + GFF4178 0.62 -0.5 82,702 - GFF4178 0.62 +1.6 82,702 - GFF4178 0.62 +1.6 82,785 + GFF4178 0.71 -0.5 82,786 - GFF4178 0.72 -0.4 82,796 + GFF4178 0.73 +0.4 82,844 + GFF4178 0.78 -2.4 82,989 + -0.7 82,989 + +0.2 83,089 - +0.8 83,089 - +0.2
Or see this region's nucleotide sequence