Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4107 and GFF4108 are separated by 7 nucleotides GFF4108 and GFF4109 overlap by 4 nucleotides GFF4109 and GFF4110 overlap by 4 nucleotides GFF4110 and GFF4111 are separated by 95 nucleotides
GFF4107 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 10,356 to 11,159
GFF4107
GFF4108 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 11,167 to 11,913
GFF4108
GFF4109 - Aspartate aminotransferase (EC 2.6.1.1), at 11,910 to 13,148
GFF4109
GFF4110 - ABC-type amino acid transport/signal transduction system, periplasmic component/domain, at 13,145 to 13,969
GFF4110
GFF4111 - ABC-type amino acid transport/signal transduction system, periplasmic component/domain, at 14,065 to 14,913
GFF4111
Position (kb)
11
12
13
14 Strain fitness (log2 ratio)
-1
0
1 at 11.645 kb on + strand, within GFF4108 at 11.646 kb on - strand, within GFF4108 at 11.853 kb on - strand at 12.004 kb on + strand at 12.005 kb on - strand at 12.143 kb on - strand, within GFF4109 at 12.230 kb on - strand, within GFF4109 at 12.230 kb on - strand, within GFF4109 at 12.404 kb on - strand, within GFF4109 at 12.404 kb on - strand, within GFF4109 at 12.584 kb on - strand, within GFF4109 at 12.884 kb on - strand, within GFF4109
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 11,645 + GFF4108 0.64 -1.3 11,646 - GFF4108 0.64 -0.7 11,853 - -0.8 12,004 + -0.3 12,005 - +0.4 12,143 - GFF4109 0.19 -0.2 12,230 - GFF4109 0.26 -0.3 12,230 - GFF4109 0.26 -0.6 12,404 - GFF4109 0.40 -1.4 12,404 - GFF4109 0.40 -1.3 12,584 - GFF4109 0.54 +0.1 12,884 - GFF4109 0.79 +0.1
Or see this region's nucleotide sequence