Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4044 and GFF4045 are separated by 118 nucleotides GFF4045 and GFF4046 are separated by 66 nucleotides GFF4046 and GFF4047 are separated by 12 nucleotides
GFF4044 - hypothetical protein, at 255,910 to 256,335
GFF4044
GFF4045 - no description, at 256,454 to 256,873
GFF4045
GFF4046 - Cobalt/zinc/cadmium efflux RND transporter, transmembrane protein CzcA, at 256,940 to 260,188
GFF4046
GFF4047 - Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein CzcB, at 260,201 to 261,796
GFF4047
Position (kb) 256
257
258
259
260
261 Strain fitness (log2 ratio) -2
-1
0
1
2 at 256.260 kb on + strand, within GFF4044 at 256.364 kb on + strand at 256.365 kb on - strand at 256.421 kb on + strand at 256.421 kb on + strand at 256.422 kb on - strand at 256.422 kb on - strand at 256.433 kb on + strand at 256.433 kb on + strand at 256.575 kb on - strand, within GFF4045 at 256.611 kb on - strand, within GFF4045 at 256.611 kb on - strand, within GFF4045 at 256.611 kb on - strand, within GFF4045 at 256.668 kb on + strand, within GFF4045 at 256.668 kb on + strand, within GFF4045 at 256.669 kb on - strand, within GFF4045 at 256.704 kb on + strand, within GFF4045 at 256.705 kb on - strand, within GFF4045 at 256.887 kb on + strand at 256.888 kb on - strand at 256.941 kb on + strand at 257.627 kb on + strand, within GFF4046 at 258.101 kb on + strand, within GFF4046 at 258.101 kb on + strand, within GFF4046 at 258.102 kb on - strand, within GFF4046 at 258.105 kb on + strand, within GFF4046 at 258.199 kb on - strand, within GFF4046 at 258.303 kb on + strand, within GFF4046 at 258.303 kb on + strand, within GFF4046 at 258.303 kb on + strand, within GFF4046 at 258.304 kb on - strand, within GFF4046 at 258.304 kb on - strand, within GFF4046 at 258.304 kb on - strand, within GFF4046 at 258.612 kb on + strand, within GFF4046 at 258.613 kb on - strand, within GFF4046 at 258.801 kb on + strand, within GFF4046 at 259.210 kb on + strand, within GFF4046 at 259.211 kb on - strand, within GFF4046 at 259.573 kb on - strand, within GFF4046 at 259.609 kb on - strand, within GFF4046 at 259.620 kb on + strand, within GFF4046 at 259.770 kb on + strand, within GFF4046 at 259.803 kb on - strand, within GFF4046 at 259.879 kb on - strand at 260.023 kb on - strand at 260.039 kb on - strand at 260.062 kb on + strand at 260.062 kb on + strand at 260.098 kb on - strand at 260.103 kb on + strand at 260.103 kb on + strand at 260.103 kb on + strand at 260.103 kb on + strand at 260.210 kb on - strand at 260.268 kb on + strand at 260.269 kb on - strand at 260.522 kb on - strand, within GFF4047 at 260.931 kb on + strand, within GFF4047 at 260.932 kb on - strand, within GFF4047 at 260.932 kb on - strand, within GFF4047 at 261.021 kb on - strand, within GFF4047 at 261.021 kb on - strand, within GFF4047
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 256,260 + GFF4044 0.82 +0.0 256,364 + -0.1 256,365 - -0.1 256,421 + +0.7 256,421 + -0.4 256,422 - +0.1 256,422 - -1.1 256,433 + -1.2 256,433 + -0.2 256,575 - GFF4045 0.29 +0.7 256,611 - GFF4045 0.37 +0.0 256,611 - GFF4045 0.37 -0.0 256,611 - GFF4045 0.37 +0.2 256,668 + GFF4045 0.51 -0.7 256,668 + GFF4045 0.51 -0.4 256,669 - GFF4045 0.51 -0.2 256,704 + GFF4045 0.60 -1.1 256,705 - GFF4045 0.60 +0.6 256,887 + -0.1 256,888 - -0.4 256,941 + -0.3 257,627 + GFF4046 0.21 +0.3 258,101 + GFF4046 0.36 -0.6 258,101 + GFF4046 0.36 -1.1 258,102 - GFF4046 0.36 -0.4 258,105 + GFF4046 0.36 +1.0 258,199 - GFF4046 0.39 +1.1 258,303 + GFF4046 0.42 -0.3 258,303 + GFF4046 0.42 -0.3 258,303 + GFF4046 0.42 -0.2 258,304 - GFF4046 0.42 +2.0 258,304 - GFF4046 0.42 +0.0 258,304 - GFF4046 0.42 -2.0 258,612 + GFF4046 0.51 -0.4 258,613 - GFF4046 0.51 +0.1 258,801 + GFF4046 0.57 -2.0 259,210 + GFF4046 0.70 -0.4 259,211 - GFF4046 0.70 -0.1 259,573 - GFF4046 0.81 -0.3 259,609 - GFF4046 0.82 +1.1 259,620 + GFF4046 0.82 +0.2 259,770 + GFF4046 0.87 -1.6 259,803 - GFF4046 0.88 -0.2 259,879 - -1.1 260,023 - +0.8 260,039 - +0.2 260,062 + -0.8 260,062 + +0.2 260,098 - -0.4 260,103 + -1.2 260,103 + -0.8 260,103 + +0.2 260,103 + +0.3 260,210 - +0.2 260,268 + +0.8 260,269 - -1.2 260,522 - GFF4047 0.20 +0.4 260,931 + GFF4047 0.46 -0.3 260,932 - GFF4047 0.46 +0.1 260,932 - GFF4047 0.46 +1.0 261,021 - GFF4047 0.51 +0.1 261,021 - GFF4047 0.51 +0.9
Or see this region's nucleotide sequence