Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3959 and GFF3960 are separated by 60 nucleotides GFF3960 and GFF3961 are separated by 248 nucleotides GFF3961 and GFF3962 are separated by 213 nucleotides
GFF3959 - Uncharacterized integral membrane endopeptidase Bmul_2226, at 159,299 to 160,558
GFF3959
GFF3960 - 3'-to-5' oligoribonuclease (orn), at 160,619 to 161,215
GFF3960
GFF3961 - ATP-dependent RNA helicase NGO0650, at 161,464 to 163,347
GFF3961
GFF3962 - Opine dehydrogenase (EC 1.5.1.28), at 163,561 to 164,634
GFF3962
Position (kb)
161
162
163
164 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 160.552 kb on - strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.559 kb on - strand at 160.559 kb on - strand at 160.559 kb on - strand at 160.559 kb on - strand at 160.559 kb on - strand at 160.559 kb on - strand at 160.559 kb on - strand at 160.559 kb on - strand at 160.559 kb on - strand at 160.584 kb on - strand at 160.584 kb on - strand at 160.584 kb on - strand at 160.949 kb on + strand, within GFF3960 at 160.949 kb on + strand, within GFF3960 at 160.949 kb on + strand, within GFF3960 at 160.949 kb on + strand, within GFF3960 at 160.949 kb on + strand, within GFF3960 at 160.950 kb on - strand, within GFF3960 at 160.950 kb on - strand, within GFF3960 at 160.950 kb on - strand, within GFF3960 at 160.950 kb on - strand, within GFF3960 at 160.950 kb on - strand, within GFF3960 at 161.018 kb on + strand, within GFF3960 at 161.018 kb on + strand, within GFF3960 at 161.018 kb on + strand, within GFF3960 at 161.018 kb on + strand, within GFF3960 at 161.018 kb on + strand, within GFF3960 at 161.018 kb on + strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.039 kb on + strand, within GFF3960 at 161.039 kb on + strand, within GFF3960 at 161.039 kb on + strand, within GFF3960 at 161.039 kb on + strand, within GFF3960 at 161.039 kb on + strand, within GFF3960 at 161.108 kb on + strand, within GFF3960 at 161.108 kb on + strand, within GFF3960 at 161.108 kb on + strand, within GFF3960 at 161.108 kb on + strand, within GFF3960 at 161.108 kb on + strand, within GFF3960 at 161.108 kb on + strand, within GFF3960 at 161.109 kb on - strand, within GFF3960 at 161.223 kb on + strand at 161.312 kb on + strand at 161.312 kb on + strand at 161.410 kb on + strand at 161.410 kb on + strand at 161.410 kb on + strand at 163.045 kb on + strand, within GFF3961 at 163.045 kb on + strand, within GFF3961 at 163.045 kb on + strand, within GFF3961 at 163.045 kb on + strand, within GFF3961 at 163.045 kb on + strand, within GFF3961 at 163.045 kb on + strand, within GFF3961 at 163.045 kb on + strand, within GFF3961 at 163.045 kb on + strand, within GFF3961 at 163.045 kb on + strand, within GFF3961 at 163.045 kb on + strand, within GFF3961 at 163.045 kb on + strand, within GFF3961 at 163.046 kb on - strand, within GFF3961 at 163.046 kb on - strand, within GFF3961 at 163.046 kb on - strand, within GFF3961 at 163.046 kb on - strand, within GFF3961 at 163.046 kb on - strand, within GFF3961 at 163.046 kb on - strand, within GFF3961 at 163.098 kb on + strand, within GFF3961 at 163.490 kb on - strand at 163.597 kb on + strand at 163.598 kb on - strand at 163.598 kb on - strand at 163.844 kb on + strand, within GFF3962 at 163.844 kb on + strand, within GFF3962 at 163.845 kb on - strand, within GFF3962 at 163.845 kb on - strand, within GFF3962 at 163.845 kb on - strand, within GFF3962 at 163.891 kb on + strand, within GFF3962 at 163.891 kb on + strand, within GFF3962 at 163.891 kb on + strand, within GFF3962 at 163.891 kb on + strand, within GFF3962 at 163.892 kb on - strand, within GFF3962 at 163.892 kb on - strand, within GFF3962 at 163.892 kb on - strand, within GFF3962 at 163.892 kb on - strand, within GFF3962 at 163.892 kb on - strand, within GFF3962 at 163.966 kb on + strand, within GFF3962 at 163.966 kb on + strand, within GFF3962 at 163.967 kb on - strand, within GFF3962 at 164.039 kb on - strand, within GFF3962 at 164.086 kb on + strand, within GFF3962 at 164.087 kb on - strand, within GFF3962 at 164.087 kb on - strand, within GFF3962 at 164.311 kb on + strand, within GFF3962 at 164.312 kb on - strand, within GFF3962 at 164.329 kb on + strand, within GFF3962 at 164.329 kb on + strand, within GFF3962 at 164.329 kb on + strand, within GFF3962 at 164.329 kb on + strand, within GFF3962 at 164.330 kb on - strand, within GFF3962 at 164.330 kb on - strand, within GFF3962 at 164.330 kb on - strand, within GFF3962 at 164.330 kb on - strand, within GFF3962
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 160,552 - -1.3 160,558 + +0.3 160,558 + +0.2 160,558 + +1.3 160,558 + -1.0 160,558 + -0.6 160,558 + -0.4 160,558 + +0.9 160,558 + +0.3 160,558 + -0.3 160,558 + +0.1 160,559 - +1.4 160,559 - -0.2 160,559 - +0.5 160,559 - -0.1 160,559 - +0.3 160,559 - -1.1 160,559 - -0.3 160,559 - -0.7 160,559 - -0.7 160,584 - +1.6 160,584 - -0.3 160,584 - -0.7 160,949 + GFF3960 0.55 -1.2 160,949 + GFF3960 0.55 -1.6 160,949 + GFF3960 0.55 +0.1 160,949 + GFF3960 0.55 +0.5 160,949 + GFF3960 0.55 -1.4 160,950 - GFF3960 0.55 -0.6 160,950 - GFF3960 0.55 +0.1 160,950 - GFF3960 0.55 -1.3 160,950 - GFF3960 0.55 -0.6 160,950 - GFF3960 0.55 +0.7 161,018 + GFF3960 0.67 +0.0 161,018 + GFF3960 0.67 -0.5 161,018 + GFF3960 0.67 +0.6 161,018 + GFF3960 0.67 +0.1 161,018 + GFF3960 0.67 -1.4 161,018 + GFF3960 0.67 +0.0 161,019 - GFF3960 0.67 -0.4 161,019 - GFF3960 0.67 +0.3 161,019 - GFF3960 0.67 -0.4 161,019 - GFF3960 0.67 +2.6 161,019 - GFF3960 0.67 -0.8 161,019 - GFF3960 0.67 -1.4 161,019 - GFF3960 0.67 -0.6 161,019 - GFF3960 0.67 +0.0 161,019 - GFF3960 0.67 -0.6 161,019 - GFF3960 0.67 -0.4 161,039 + GFF3960 0.70 -2.0 161,039 + GFF3960 0.70 +0.1 161,039 + GFF3960 0.70 -0.6 161,039 + GFF3960 0.70 -0.0 161,039 + GFF3960 0.70 -0.6 161,108 + GFF3960 0.82 -0.9 161,108 + GFF3960 0.82 +0.2 161,108 + GFF3960 0.82 +1.6 161,108 + GFF3960 0.82 -1.0 161,108 + GFF3960 0.82 -0.1 161,108 + GFF3960 0.82 -1.2 161,109 - GFF3960 0.82 -1.2 161,223 + +2.2 161,312 + -1.0 161,312 + -0.1 161,410 + -2.4 161,410 + +0.1 161,410 + -0.2 163,045 + GFF3961 0.84 +0.6 163,045 + GFF3961 0.84 +1.2 163,045 + GFF3961 0.84 +0.3 163,045 + GFF3961 0.84 -0.4 163,045 + GFF3961 0.84 +1.4 163,045 + GFF3961 0.84 +0.4 163,045 + GFF3961 0.84 +0.0 163,045 + GFF3961 0.84 -0.7 163,045 + GFF3961 0.84 -0.4 163,045 + GFF3961 0.84 -0.7 163,045 + GFF3961 0.84 +0.4 163,046 - GFF3961 0.84 -0.9 163,046 - GFF3961 0.84 -0.9 163,046 - GFF3961 0.84 +0.5 163,046 - GFF3961 0.84 +0.4 163,046 - GFF3961 0.84 -0.3 163,046 - GFF3961 0.84 -0.0 163,098 + GFF3961 0.87 +0.3 163,490 - +0.6 163,597 + +0.0 163,598 - -0.3 163,598 - -1.2 163,844 + GFF3962 0.26 -0.6 163,844 + GFF3962 0.26 -0.3 163,845 - GFF3962 0.26 -1.0 163,845 - GFF3962 0.26 +0.7 163,845 - GFF3962 0.26 -0.3 163,891 + GFF3962 0.31 -0.3 163,891 + GFF3962 0.31 -0.2 163,891 + GFF3962 0.31 +0.8 163,891 + GFF3962 0.31 -0.3 163,892 - GFF3962 0.31 +0.9 163,892 - GFF3962 0.31 +0.2 163,892 - GFF3962 0.31 -0.7 163,892 - GFF3962 0.31 +0.7 163,892 - GFF3962 0.31 -1.1 163,966 + GFF3962 0.38 -0.4 163,966 + GFF3962 0.38 -0.4 163,967 - GFF3962 0.38 +0.4 164,039 - GFF3962 0.45 +0.0 164,086 + GFF3962 0.49 +0.7 164,087 - GFF3962 0.49 +0.9 164,087 - GFF3962 0.49 -0.2 164,311 + GFF3962 0.70 +0.0 164,312 - GFF3962 0.70 -0.1 164,329 + GFF3962 0.72 -1.0 164,329 + GFF3962 0.72 -1.2 164,329 + GFF3962 0.72 +1.6 164,329 + GFF3962 0.72 -0.5 164,330 - GFF3962 0.72 -0.9 164,330 - GFF3962 0.72 -0.6 164,330 - GFF3962 0.72 +0.8 164,330 - GFF3962 0.72 +1.6
Or see this region's nucleotide sequence