Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3957 and GFF3958 are separated by 12 nucleotides GFF3958 and GFF3959 are separated by 30 nucleotides GFF3959 and GFF3960 are separated by 60 nucleotides GFF3960 and GFF3961 are separated by 248 nucleotides
GFF3957 - Ribosome small subunit biogenesis RbfA-release protein RsgA, at 157,937 to 158,896
GFF3957
GFF3958 - Pterin-4-alpha-carbinolamine dehydratase (EC 4.2.1.96), at 158,909 to 159,268
GFF3958
GFF3959 - Uncharacterized integral membrane endopeptidase Bmul_2226, at 159,299 to 160,558
GFF3959
GFF3960 - 3'-to-5' oligoribonuclease (orn), at 160,619 to 161,215
GFF3960
GFF3961 - ATP-dependent RNA helicase NGO0650, at 161,464 to 163,347
GFF3961
Position (kb) 159
160
161 Strain fitness (log2 ratio) -2
-1
0
1
2 at 158.385 kb on + strand, within GFF3957 at 158.891 kb on - strand at 158.904 kb on + strand at 158.905 kb on - strand at 158.905 kb on - strand at 159.027 kb on + strand, within GFF3958 at 159.027 kb on + strand, within GFF3958 at 159.027 kb on + strand, within GFF3958 at 159.027 kb on + strand, within GFF3958 at 159.027 kb on + strand, within GFF3958 at 159.027 kb on + strand, within GFF3958 at 159.028 kb on - strand, within GFF3958 at 159.028 kb on - strand, within GFF3958 at 159.108 kb on + strand, within GFF3958 at 159.108 kb on + strand, within GFF3958 at 159.109 kb on - strand, within GFF3958 at 159.349 kb on - strand at 159.351 kb on + strand at 159.351 kb on + strand at 159.351 kb on + strand at 159.351 kb on + strand at 159.352 kb on - strand at 159.352 kb on - strand at 159.352 kb on - strand at 159.352 kb on - strand at 159.352 kb on - strand at 159.352 kb on - strand at 159.403 kb on - strand at 159.606 kb on + strand, within GFF3959 at 159.606 kb on + strand, within GFF3959 at 159.607 kb on - strand, within GFF3959 at 159.774 kb on + strand, within GFF3959 at 159.775 kb on - strand, within GFF3959 at 159.775 kb on - strand, within GFF3959 at 159.775 kb on - strand, within GFF3959 at 159.775 kb on - strand, within GFF3959 at 159.775 kb on - strand, within GFF3959 at 159.813 kb on + strand, within GFF3959 at 159.814 kb on - strand, within GFF3959 at 159.814 kb on - strand, within GFF3959 at 159.814 kb on - strand, within GFF3959 at 159.814 kb on - strand, within GFF3959 at 159.814 kb on - strand, within GFF3959 at 159.970 kb on - strand, within GFF3959 at 159.970 kb on - strand, within GFF3959 at 160.383 kb on + strand, within GFF3959 at 160.383 kb on + strand, within GFF3959 at 160.383 kb on + strand, within GFF3959 at 160.384 kb on - strand, within GFF3959 at 160.384 kb on - strand, within GFF3959 at 160.384 kb on - strand, within GFF3959 at 160.384 kb on - strand, within GFF3959 at 160.552 kb on - strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.558 kb on + strand at 160.559 kb on - strand at 160.559 kb on - strand at 160.559 kb on - strand at 160.559 kb on - strand at 160.559 kb on - strand at 160.559 kb on - strand at 160.559 kb on - strand at 160.559 kb on - strand at 160.559 kb on - strand at 160.584 kb on - strand at 160.584 kb on - strand at 160.584 kb on - strand at 160.949 kb on + strand, within GFF3960 at 160.949 kb on + strand, within GFF3960 at 160.949 kb on + strand, within GFF3960 at 160.949 kb on + strand, within GFF3960 at 160.949 kb on + strand, within GFF3960 at 160.950 kb on - strand, within GFF3960 at 160.950 kb on - strand, within GFF3960 at 160.950 kb on - strand, within GFF3960 at 160.950 kb on - strand, within GFF3960 at 160.950 kb on - strand, within GFF3960 at 161.018 kb on + strand, within GFF3960 at 161.018 kb on + strand, within GFF3960 at 161.018 kb on + strand, within GFF3960 at 161.018 kb on + strand, within GFF3960 at 161.018 kb on + strand, within GFF3960 at 161.018 kb on + strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.019 kb on - strand, within GFF3960 at 161.039 kb on + strand, within GFF3960 at 161.039 kb on + strand, within GFF3960 at 161.039 kb on + strand, within GFF3960 at 161.039 kb on + strand, within GFF3960 at 161.039 kb on + strand, within GFF3960 at 161.108 kb on + strand, within GFF3960 at 161.108 kb on + strand, within GFF3960 at 161.108 kb on + strand, within GFF3960 at 161.108 kb on + strand, within GFF3960 at 161.108 kb on + strand, within GFF3960 at 161.108 kb on + strand, within GFF3960 at 161.109 kb on - strand, within GFF3960 at 161.223 kb on + strand at 161.312 kb on + strand at 161.312 kb on + strand at 161.410 kb on + strand at 161.410 kb on + strand at 161.410 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 158,385 + GFF3957 0.47 -0.8 158,891 - -0.6 158,904 + -1.0 158,905 - -1.3 158,905 - -0.6 159,027 + GFF3958 0.33 -1.0 159,027 + GFF3958 0.33 -0.2 159,027 + GFF3958 0.33 -0.3 159,027 + GFF3958 0.33 -0.5 159,027 + GFF3958 0.33 -1.0 159,027 + GFF3958 0.33 +1.0 159,028 - GFF3958 0.33 -0.8 159,028 - GFF3958 0.33 -1.1 159,108 + GFF3958 0.55 -0.1 159,108 + GFF3958 0.55 -0.1 159,109 - GFF3958 0.56 +0.1 159,349 - -0.5 159,351 + +0.1 159,351 + +0.2 159,351 + +0.1 159,351 + +0.6 159,352 - -1.0 159,352 - -0.3 159,352 - +0.1 159,352 - -0.2 159,352 - -0.1 159,352 - -0.3 159,403 - -0.7 159,606 + GFF3959 0.24 -0.8 159,606 + GFF3959 0.24 -0.1 159,607 - GFF3959 0.24 +0.0 159,774 + GFF3959 0.38 -1.7 159,775 - GFF3959 0.38 -0.2 159,775 - GFF3959 0.38 -1.0 159,775 - GFF3959 0.38 -0.5 159,775 - GFF3959 0.38 -0.9 159,775 - GFF3959 0.38 -1.4 159,813 + GFF3959 0.41 -1.2 159,814 - GFF3959 0.41 +0.3 159,814 - GFF3959 0.41 +0.0 159,814 - GFF3959 0.41 -1.1 159,814 - GFF3959 0.41 -0.4 159,814 - GFF3959 0.41 +1.3 159,970 - GFF3959 0.53 -1.4 159,970 - GFF3959 0.53 -1.1 160,383 + GFF3959 0.86 -0.9 160,383 + GFF3959 0.86 -1.4 160,383 + GFF3959 0.86 +0.0 160,384 - GFF3959 0.86 -1.8 160,384 - GFF3959 0.86 -1.0 160,384 - GFF3959 0.86 -2.2 160,384 - GFF3959 0.86 -1.8 160,552 - -1.3 160,558 + +0.3 160,558 + +0.2 160,558 + +1.3 160,558 + -1.0 160,558 + -0.6 160,558 + -0.4 160,558 + +0.9 160,558 + +0.3 160,558 + -0.3 160,558 + +0.1 160,559 - +1.4 160,559 - -0.2 160,559 - +0.5 160,559 - -0.1 160,559 - +0.3 160,559 - -1.1 160,559 - -0.3 160,559 - -0.7 160,559 - -0.7 160,584 - +1.6 160,584 - -0.3 160,584 - -0.7 160,949 + GFF3960 0.55 -1.2 160,949 + GFF3960 0.55 -1.6 160,949 + GFF3960 0.55 +0.1 160,949 + GFF3960 0.55 +0.5 160,949 + GFF3960 0.55 -1.4 160,950 - GFF3960 0.55 -0.6 160,950 - GFF3960 0.55 +0.1 160,950 - GFF3960 0.55 -1.3 160,950 - GFF3960 0.55 -0.6 160,950 - GFF3960 0.55 +0.7 161,018 + GFF3960 0.67 +0.0 161,018 + GFF3960 0.67 -0.5 161,018 + GFF3960 0.67 +0.6 161,018 + GFF3960 0.67 +0.1 161,018 + GFF3960 0.67 -1.4 161,018 + GFF3960 0.67 +0.0 161,019 - GFF3960 0.67 -0.4 161,019 - GFF3960 0.67 +0.3 161,019 - GFF3960 0.67 -0.4 161,019 - GFF3960 0.67 +2.6 161,019 - GFF3960 0.67 -0.8 161,019 - GFF3960 0.67 -1.4 161,019 - GFF3960 0.67 -0.6 161,019 - GFF3960 0.67 +0.0 161,019 - GFF3960 0.67 -0.6 161,019 - GFF3960 0.67 -0.4 161,039 + GFF3960 0.70 -2.0 161,039 + GFF3960 0.70 +0.1 161,039 + GFF3960 0.70 -0.6 161,039 + GFF3960 0.70 -0.0 161,039 + GFF3960 0.70 -0.6 161,108 + GFF3960 0.82 -0.9 161,108 + GFF3960 0.82 +0.2 161,108 + GFF3960 0.82 +1.6 161,108 + GFF3960 0.82 -1.0 161,108 + GFF3960 0.82 -0.1 161,108 + GFF3960 0.82 -1.2 161,109 - GFF3960 0.82 -1.2 161,223 + +2.2 161,312 + -1.0 161,312 + -0.1 161,410 + -2.4 161,410 + +0.1 161,410 + -0.2
Or see this region's nucleotide sequence