Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3901 and GFF3902 are separated by 12 nucleotides GFF3902 and GFF3903 overlap by 4 nucleotides GFF3903 and GFF3904 are separated by 34 nucleotides GFF3904 and GFF3905 are separated by 80 nucleotides
GFF3901 - Malate dehydrogenase (EC 1.1.1.37), at 102,793 to 103,779
GFF3901
GFF3902 - Trans-aconitate 2-methyltransferase (EC 2.1.1.144), at 103,792 to 104,583
GFF3902
GFF3903 - Similar to citrate lyase beta chain, 10, at 104,580 to 105,581
GFF3903
GFF3904 - Probable signal peptide protein, at 105,616 to 106,188
GFF3904
GFF3905 - Aconitate hydratase 2 (EC 4.2.1.3), at 106,269 to 108,854
GFF3905
Position (kb)
104
105
106 Strain fitness (log2 ratio)
-2
-1
0
1 at 103.777 kb on + strand at 103.777 kb on + strand at 103.777 kb on + strand at 103.777 kb on + strand at 103.777 kb on + strand at 103.778 kb on - strand at 103.778 kb on - strand at 103.816 kb on + strand at 103.816 kb on + strand at 103.816 kb on + strand at 103.817 kb on - strand at 104.092 kb on + strand, within GFF3902 at 104.092 kb on + strand, within GFF3902 at 104.092 kb on + strand, within GFF3902 at 104.092 kb on + strand, within GFF3902 at 104.092 kb on + strand, within GFF3902 at 104.093 kb on - strand, within GFF3902 at 104.093 kb on - strand, within GFF3902 at 104.093 kb on - strand, within GFF3902 at 104.093 kb on - strand, within GFF3902 at 104.311 kb on + strand, within GFF3902 at 104.314 kb on + strand, within GFF3902 at 104.314 kb on + strand, within GFF3902 at 104.314 kb on + strand, within GFF3902 at 104.314 kb on + strand, within GFF3902 at 104.314 kb on + strand, within GFF3902 at 104.314 kb on + strand, within GFF3902 at 104.314 kb on + strand, within GFF3902 at 104.315 kb on - strand, within GFF3902 at 104.315 kb on - strand, within GFF3902 at 104.315 kb on - strand, within GFF3902 at 104.315 kb on - strand, within GFF3902 at 104.365 kb on + strand, within GFF3902 at 104.365 kb on + strand, within GFF3902 at 104.365 kb on + strand, within GFF3902 at 104.365 kb on + strand, within GFF3902 at 104.365 kb on + strand, within GFF3902 at 104.365 kb on + strand, within GFF3902 at 104.365 kb on + strand, within GFF3902 at 104.365 kb on + strand, within GFF3902 at 104.366 kb on - strand, within GFF3902 at 104.366 kb on - strand, within GFF3902 at 104.366 kb on - strand, within GFF3902 at 104.366 kb on - strand, within GFF3902 at 104.366 kb on - strand, within GFF3902 at 104.366 kb on - strand, within GFF3902 at 104.366 kb on - strand, within GFF3902 at 104.366 kb on - strand, within GFF3902 at 104.366 kb on - strand, within GFF3902 at 104.366 kb on - strand, within GFF3902 at 104.366 kb on - strand, within GFF3902 at 104.467 kb on + strand, within GFF3902 at 104.467 kb on + strand, within GFF3902 at 104.468 kb on - strand, within GFF3902 at 104.468 kb on - strand, within GFF3902 at 104.468 kb on - strand, within GFF3902 at 104.468 kb on - strand, within GFF3902 at 104.479 kb on + strand, within GFF3902 at 104.480 kb on - strand, within GFF3902 at 104.480 kb on - strand, within GFF3902 at 104.661 kb on + strand at 104.661 kb on + strand at 104.661 kb on + strand at 104.662 kb on - strand at 104.662 kb on - strand at 104.662 kb on - strand at 104.662 kb on - strand at 104.662 kb on - strand at 104.662 kb on - strand at 105.112 kb on - strand, within GFF3903 at 105.228 kb on + strand, within GFF3903 at 105.228 kb on + strand, within GFF3903 at 105.229 kb on - strand, within GFF3903 at 105.229 kb on - strand, within GFF3903 at 105.229 kb on - strand, within GFF3903 at 105.421 kb on + strand, within GFF3903 at 105.421 kb on + strand, within GFF3903 at 105.421 kb on + strand, within GFF3903 at 105.421 kb on + strand, within GFF3903 at 105.421 kb on + strand, within GFF3903 at 105.422 kb on - strand, within GFF3903 at 105.422 kb on - strand, within GFF3903 at 105.422 kb on - strand, within GFF3903 at 105.422 kb on - strand, within GFF3903 at 105.486 kb on + strand at 105.487 kb on - strand at 105.487 kb on - strand at 105.591 kb on + strand at 105.961 kb on + strand, within GFF3904 at 105.961 kb on + strand, within GFF3904 at 105.962 kb on - strand, within GFF3904 at 106.186 kb on + strand at 106.186 kb on + strand at 106.186 kb on + strand at 106.187 kb on - strand at 106.187 kb on - strand at 106.187 kb on - strand at 106.187 kb on - strand at 106.187 kb on - strand at 106.187 kb on - strand at 106.192 kb on + strand at 106.281 kb on + strand at 106.281 kb on + strand at 106.281 kb on + strand at 106.282 kb on - strand at 106.420 kb on - strand at 106.467 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 103,777 + +0.4 103,777 + +1.6 103,777 + -1.0 103,777 + -0.5 103,777 + +1.2 103,778 - +0.8 103,778 - +0.4 103,816 + +0.4 103,816 + -0.3 103,816 + +0.4 103,817 - -0.9 104,092 + GFF3902 0.38 -1.0 104,092 + GFF3902 0.38 -0.4 104,092 + GFF3902 0.38 +0.1 104,092 + GFF3902 0.38 +0.0 104,092 + GFF3902 0.38 -0.2 104,093 - GFF3902 0.38 +0.4 104,093 - GFF3902 0.38 -0.1 104,093 - GFF3902 0.38 -0.9 104,093 - GFF3902 0.38 -1.3 104,311 + GFF3902 0.66 -0.6 104,314 + GFF3902 0.66 -0.4 104,314 + GFF3902 0.66 +1.6 104,314 + GFF3902 0.66 -0.7 104,314 + GFF3902 0.66 -1.1 104,314 + GFF3902 0.66 +1.0 104,314 + GFF3902 0.66 +0.6 104,314 + GFF3902 0.66 -1.8 104,315 - GFF3902 0.66 -0.1 104,315 - GFF3902 0.66 -0.0 104,315 - GFF3902 0.66 -0.7 104,315 - GFF3902 0.66 +0.7 104,365 + GFF3902 0.72 -0.8 104,365 + GFF3902 0.72 -1.7 104,365 + GFF3902 0.72 +0.4 104,365 + GFF3902 0.72 -2.1 104,365 + GFF3902 0.72 -0.2 104,365 + GFF3902 0.72 +0.0 104,365 + GFF3902 0.72 -0.5 104,365 + GFF3902 0.72 -0.2 104,366 - GFF3902 0.72 -0.4 104,366 - GFF3902 0.72 -0.7 104,366 - GFF3902 0.72 -0.5 104,366 - GFF3902 0.72 -0.6 104,366 - GFF3902 0.72 -0.2 104,366 - GFF3902 0.72 +1.6 104,366 - GFF3902 0.72 -1.0 104,366 - GFF3902 0.72 -0.8 104,366 - GFF3902 0.72 +0.4 104,366 - GFF3902 0.72 -0.5 104,366 - GFF3902 0.72 -0.2 104,467 + GFF3902 0.85 -0.5 104,467 + GFF3902 0.85 -1.2 104,468 - GFF3902 0.85 +0.5 104,468 - GFF3902 0.85 -0.5 104,468 - GFF3902 0.85 -0.7 104,468 - GFF3902 0.85 -1.0 104,479 + GFF3902 0.87 +0.3 104,480 - GFF3902 0.87 -0.7 104,480 - GFF3902 0.87 +0.4 104,661 + -0.3 104,661 + +0.5 104,661 + -0.4 104,662 - -0.3 104,662 - +0.4 104,662 - -0.6 104,662 - +0.8 104,662 - -0.4 104,662 - -0.9 105,112 - GFF3903 0.53 -0.1 105,228 + GFF3903 0.65 +0.3 105,228 + GFF3903 0.65 -0.3 105,229 - GFF3903 0.65 -1.0 105,229 - GFF3903 0.65 -0.6 105,229 - GFF3903 0.65 -1.0 105,421 + GFF3903 0.84 +0.4 105,421 + GFF3903 0.84 +0.1 105,421 + GFF3903 0.84 -0.6 105,421 + GFF3903 0.84 -0.6 105,421 + GFF3903 0.84 -1.6 105,422 - GFF3903 0.84 +0.1 105,422 - GFF3903 0.84 +0.3 105,422 - GFF3903 0.84 -0.7 105,422 - GFF3903 0.84 -0.8 105,486 + -0.6 105,487 - +0.1 105,487 - -0.8 105,591 + -2.1 105,961 + GFF3904 0.60 -0.4 105,961 + GFF3904 0.60 -1.5 105,962 - GFF3904 0.60 -0.4 106,186 + -0.2 106,186 + +1.3 106,186 + +0.0 106,187 - -0.4 106,187 - +0.6 106,187 - -0.5 106,187 - +0.0 106,187 - -1.3 106,187 - -0.7 106,192 + +0.5 106,281 + +0.9 106,281 + -0.3 106,281 + -0.7 106,282 - +0.0 106,420 - -0.2 106,467 + +0.2
Or see this region's nucleotide sequence