Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3884 and GFF3885 overlap by 4 nucleotides GFF3885 and GFF3886 are separated by 30 nucleotides GFF3886 and GFF3887 are separated by 168 nucleotides
GFF3884 - Phosphoribosylanthranilate isomerase (EC 5.3.1.24), at 81,230 to 81,937
GFF3884
GFF3885 - tRNA pseudouridine(38-40) synthase (EC 5.4.99.12), at 81,934 to 82,746
GFF3885
GFF3886 - Putative transmembrane protein, at 82,777 to 85,605
GFF3886
GFF3887 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 85,774 to 86,943
GFF3887
Position (kb)
82
83
84
85
86 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.950 kb on + strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 81.951 kb on - strand at 82.007 kb on + strand at 82.008 kb on - strand at 82.025 kb on + strand, within GFF3885 at 82.025 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.415 kb on + strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.416 kb on - strand, within GFF3885 at 82.421 kb on + strand, within GFF3885 at 82.421 kb on + strand, within GFF3885 at 82.421 kb on + strand, within GFF3885 at 82.422 kb on - strand, within GFF3885 at 82.422 kb on - strand, within GFF3885 at 82.706 kb on + strand at 82.706 kb on + strand at 82.707 kb on - strand at 82.707 kb on - strand at 82.707 kb on - strand at 82.707 kb on - strand at 82.707 kb on - strand at 82.721 kb on + strand at 82.722 kb on - strand at 82.722 kb on - strand at 82.732 kb on + strand at 82.733 kb on - strand at 82.733 kb on - strand at 82.733 kb on - strand at 82.733 kb on - strand at 83.237 kb on + strand, within GFF3886 at 83.238 kb on - strand, within GFF3886 at 83.238 kb on - strand, within GFF3886 at 83.238 kb on - strand, within GFF3886 at 83.498 kb on + strand, within GFF3886 at 83.499 kb on - strand, within GFF3886 at 83.499 kb on - strand, within GFF3886 at 83.499 kb on - strand, within GFF3886 at 83.499 kb on - strand, within GFF3886 at 83.603 kb on + strand, within GFF3886 at 83.604 kb on - strand, within GFF3886 at 83.604 kb on - strand, within GFF3886 at 83.604 kb on - strand, within GFF3886 at 83.604 kb on - strand, within GFF3886 at 83.604 kb on - strand, within GFF3886 at 83.632 kb on + strand, within GFF3886 at 83.723 kb on + strand, within GFF3886 at 83.724 kb on - strand, within GFF3886 at 83.724 kb on - strand, within GFF3886 at 83.732 kb on + strand, within GFF3886 at 83.732 kb on + strand, within GFF3886 at 83.733 kb on - strand, within GFF3886 at 83.733 kb on - strand, within GFF3886 at 83.733 kb on - strand, within GFF3886 at 83.733 kb on - strand, within GFF3886 at 83.733 kb on - strand, within GFF3886 at 83.733 kb on - strand, within GFF3886 at 83.789 kb on + strand, within GFF3886 at 83.789 kb on + strand, within GFF3886 at 83.790 kb on - strand, within GFF3886 at 83.790 kb on - strand, within GFF3886 at 83.790 kb on - strand, within GFF3886 at 83.936 kb on + strand, within GFF3886 at 83.936 kb on + strand, within GFF3886 at 83.936 kb on + strand, within GFF3886 at 83.937 kb on - strand, within GFF3886 at 83.937 kb on - strand, within GFF3886 at 84.414 kb on + strand, within GFF3886 at 84.414 kb on + strand, within GFF3886 at 84.415 kb on - strand, within GFF3886 at 84.415 kb on - strand, within GFF3886 at 84.415 kb on - strand, within GFF3886 at 84.415 kb on - strand, within GFF3886 at 84.618 kb on + strand, within GFF3886 at 84.618 kb on + strand, within GFF3886 at 84.618 kb on + strand, within GFF3886 at 84.618 kb on + strand, within GFF3886 at 84.618 kb on + strand, within GFF3886 at 84.618 kb on + strand, within GFF3886 at 84.618 kb on + strand, within GFF3886 at 84.619 kb on - strand, within GFF3886 at 84.619 kb on - strand, within GFF3886 at 84.619 kb on - strand, within GFF3886 at 84.619 kb on - strand, within GFF3886 at 84.675 kb on - strand, within GFF3886 at 85.169 kb on + strand, within GFF3886 at 85.169 kb on + strand, within GFF3886 at 85.169 kb on + strand, within GFF3886 at 85.169 kb on + strand, within GFF3886 at 85.169 kb on + strand, within GFF3886 at 85.169 kb on + strand, within GFF3886 at 85.170 kb on - strand, within GFF3886 at 85.170 kb on - strand, within GFF3886 at 85.170 kb on - strand, within GFF3886 at 85.170 kb on - strand, within GFF3886 at 85.170 kb on - strand, within GFF3886 at 85.170 kb on - strand, within GFF3886 at 85.170 kb on - strand, within GFF3886 at 85.170 kb on - strand, within GFF3886 at 85.170 kb on - strand, within GFF3886 at 85.265 kb on + strand, within GFF3886 at 85.265 kb on + strand, within GFF3886 at 85.265 kb on + strand, within GFF3886 at 85.265 kb on + strand, within GFF3886 at 85.265 kb on + strand, within GFF3886 at 85.265 kb on + strand, within GFF3886 at 85.266 kb on - strand, within GFF3886 at 85.266 kb on - strand, within GFF3886 at 85.266 kb on - strand, within GFF3886 at 85.266 kb on - strand, within GFF3886 at 85.266 kb on - strand, within GFF3886 at 85.266 kb on - strand, within GFF3886 at 85.300 kb on + strand, within GFF3886 at 85.301 kb on - strand, within GFF3886 at 85.301 kb on - strand, within GFF3886 at 85.301 kb on - strand, within GFF3886 at 85.301 kb on - strand, within GFF3886 at 85.301 kb on - strand, within GFF3886 at 85.322 kb on + strand, within GFF3886 at 85.322 kb on + strand, within GFF3886 at 85.322 kb on + strand, within GFF3886 at 85.322 kb on + strand, within GFF3886 at 85.322 kb on + strand, within GFF3886 at 85.322 kb on + strand, within GFF3886 at 85.322 kb on + strand, within GFF3886 at 85.323 kb on - strand at 85.323 kb on - strand at 85.323 kb on - strand at 85.323 kb on - strand at 85.323 kb on - strand at 85.323 kb on - strand at 85.323 kb on - strand at 85.323 kb on - strand at 85.611 kb on + strand at 85.611 kb on + strand at 85.611 kb on + strand at 85.611 kb on + strand at 85.612 kb on - strand at 85.612 kb on - strand at 85.612 kb on - strand at 85.637 kb on + strand at 85.638 kb on - strand at 85.648 kb on + strand at 85.689 kb on + strand at 85.689 kb on + strand at 85.689 kb on + strand at 85.689 kb on + strand at 85.689 kb on + strand at 85.690 kb on - strand at 85.704 kb on + strand at 85.704 kb on + strand at 85.705 kb on - strand at 85.705 kb on - strand at 85.705 kb on - strand at 85.705 kb on - strand at 85.705 kb on - strand at 85.706 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.708 kb on + strand at 85.709 kb on - strand at 85.709 kb on - strand at 85.709 kb on - strand at 85.709 kb on - strand at 85.709 kb on - strand at 85.709 kb on - strand at 85.709 kb on - strand at 85.709 kb on - strand at 85.709 kb on - strand at 85.709 kb on - strand at 85.709 kb on - strand at 85.709 kb on - strand at 85.709 kb on - strand at 85.710 kb on - strand at 85.721 kb on + strand at 85.721 kb on + strand at 85.721 kb on + strand at 85.721 kb on + strand at 85.721 kb on + strand at 85.721 kb on + strand at 85.721 kb on + strand at 85.721 kb on + strand at 85.721 kb on + strand at 85.722 kb on - strand at 85.722 kb on - strand at 85.722 kb on - strand at 85.722 kb on - strand at 85.722 kb on - strand at 85.722 kb on - strand at 85.722 kb on - strand at 85.722 kb on - strand at 85.722 kb on - strand at 85.722 kb on - strand at 85.722 kb on - strand at 85.722 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 81,950 + -0.3 81,950 + -1.6 81,950 + +0.3 81,950 + -0.7 81,950 + +0.3 81,950 + -0.5 81,950 + +0.1 81,950 + +0.6 81,950 + -0.3 81,950 + +0.7 81,951 - -0.1 81,951 - +0.9 81,951 - +0.2 81,951 - -0.6 81,951 - +0.4 81,951 - -1.8 81,951 - -0.1 81,951 - -0.1 81,951 - +0.3 81,951 - -1.3 81,951 - -1.7 81,951 - -0.1 81,951 - -0.7 81,951 - +0.1 81,951 - +2.4 81,951 - -0.1 81,951 - +0.3 82,007 + -0.1 82,008 - -0.9 82,025 + GFF3885 0.11 -1.3 82,025 + GFF3885 0.11 -0.2 82,415 + GFF3885 0.59 +0.6 82,415 + GFF3885 0.59 -0.4 82,415 + GFF3885 0.59 +0.2 82,415 + GFF3885 0.59 -2.3 82,415 + GFF3885 0.59 +0.2 82,415 + GFF3885 0.59 -1.4 82,416 - GFF3885 0.59 +0.9 82,416 - GFF3885 0.59 +0.1 82,416 - GFF3885 0.59 +0.4 82,416 - GFF3885 0.59 +0.1 82,416 - GFF3885 0.59 +0.0 82,416 - GFF3885 0.59 -2.0 82,416 - GFF3885 0.59 -0.3 82,416 - GFF3885 0.59 -1.5 82,416 - GFF3885 0.59 -0.2 82,416 - GFF3885 0.59 +0.3 82,416 - GFF3885 0.59 +1.9 82,421 + GFF3885 0.60 +0.4 82,421 + GFF3885 0.60 -0.1 82,421 + GFF3885 0.60 +0.6 82,422 - GFF3885 0.60 -1.0 82,422 - GFF3885 0.60 +0.1 82,706 + +0.3 82,706 + -0.5 82,707 - -0.8 82,707 - -0.2 82,707 - -1.8 82,707 - +1.0 82,707 - +1.0 82,721 + +0.6 82,722 - -0.4 82,722 - -0.5 82,732 + -1.6 82,733 - -0.4 82,733 - +0.0 82,733 - -0.9 82,733 - -0.5 83,237 + GFF3886 0.16 +0.9 83,238 - GFF3886 0.16 -0.5 83,238 - GFF3886 0.16 -0.0 83,238 - GFF3886 0.16 -1.0 83,498 + GFF3886 0.25 -1.0 83,499 - GFF3886 0.26 -1.4 83,499 - GFF3886 0.26 -0.5 83,499 - GFF3886 0.26 -1.0 83,499 - GFF3886 0.26 -0.8 83,603 + GFF3886 0.29 -0.6 83,604 - GFF3886 0.29 -1.2 83,604 - GFF3886 0.29 -2.7 83,604 - GFF3886 0.29 -0.6 83,604 - GFF3886 0.29 -2.5 83,604 - GFF3886 0.29 -0.5 83,632 + GFF3886 0.30 -1.8 83,723 + GFF3886 0.33 -1.0 83,724 - GFF3886 0.33 -0.9 83,724 - GFF3886 0.33 -0.7 83,732 + GFF3886 0.34 -0.5 83,732 + GFF3886 0.34 +0.0 83,733 - GFF3886 0.34 -1.2 83,733 - GFF3886 0.34 -1.2 83,733 - GFF3886 0.34 -0.8 83,733 - GFF3886 0.34 -1.5 83,733 - GFF3886 0.34 -1.4 83,733 - GFF3886 0.34 +2.0 83,789 + GFF3886 0.36 +0.9 83,789 + GFF3886 0.36 -0.7 83,790 - GFF3886 0.36 -1.0 83,790 - GFF3886 0.36 -1.3 83,790 - GFF3886 0.36 -0.7 83,936 + GFF3886 0.41 -1.6 83,936 + GFF3886 0.41 +0.4 83,936 + GFF3886 0.41 -1.1 83,937 - GFF3886 0.41 -1.3 83,937 - GFF3886 0.41 -0.6 84,414 + GFF3886 0.58 -1.0 84,414 + GFF3886 0.58 -1.4 84,415 - GFF3886 0.58 -0.6 84,415 - GFF3886 0.58 -1.8 84,415 - GFF3886 0.58 -1.8 84,415 - GFF3886 0.58 -1.6 84,618 + GFF3886 0.65 -1.0 84,618 + GFF3886 0.65 -1.8 84,618 + GFF3886 0.65 -1.6 84,618 + GFF3886 0.65 -2.0 84,618 + GFF3886 0.65 -0.9 84,618 + GFF3886 0.65 +0.9 84,618 + GFF3886 0.65 -1.7 84,619 - GFF3886 0.65 -0.8 84,619 - GFF3886 0.65 -1.4 84,619 - GFF3886 0.65 -1.4 84,619 - GFF3886 0.65 +1.8 84,675 - GFF3886 0.67 -0.4 85,169 + GFF3886 0.85 -1.1 85,169 + GFF3886 0.85 -1.0 85,169 + GFF3886 0.85 -0.4 85,169 + GFF3886 0.85 -1.4 85,169 + GFF3886 0.85 +0.3 85,169 + GFF3886 0.85 -0.4 85,170 - GFF3886 0.85 -2.3 85,170 - GFF3886 0.85 -0.6 85,170 - GFF3886 0.85 -0.7 85,170 - GFF3886 0.85 +0.5 85,170 - GFF3886 0.85 +1.6 85,170 - GFF3886 0.85 -0.7 85,170 - GFF3886 0.85 -1.6 85,170 - GFF3886 0.85 -0.3 85,170 - GFF3886 0.85 -1.4 85,265 + GFF3886 0.88 -0.6 85,265 + GFF3886 0.88 -1.6 85,265 + GFF3886 0.88 -0.4 85,265 + GFF3886 0.88 -0.3 85,265 + GFF3886 0.88 -0.3 85,265 + GFF3886 0.88 -1.6 85,266 - GFF3886 0.88 -0.5 85,266 - GFF3886 0.88 -2.4 85,266 - GFF3886 0.88 -1.2 85,266 - GFF3886 0.88 -0.3 85,266 - GFF3886 0.88 +0.0 85,266 - GFF3886 0.88 -1.4 85,300 + GFF3886 0.89 -0.4 85,301 - GFF3886 0.89 -1.5 85,301 - GFF3886 0.89 -1.0 85,301 - GFF3886 0.89 -1.4 85,301 - GFF3886 0.89 -0.4 85,301 - GFF3886 0.89 -0.5 85,322 + GFF3886 0.90 -1.1 85,322 + GFF3886 0.90 -0.4 85,322 + GFF3886 0.90 -0.6 85,322 + GFF3886 0.90 +0.0 85,322 + GFF3886 0.90 +0.6 85,322 + GFF3886 0.90 -0.7 85,322 + GFF3886 0.90 -1.0 85,323 - -1.8 85,323 - -1.0 85,323 - -1.3 85,323 - +0.1 85,323 - -0.9 85,323 - -1.6 85,323 - -0.4 85,323 - -1.4 85,611 + -0.2 85,611 + -1.6 85,611 + -0.5 85,611 + -0.9 85,612 - +0.6 85,612 - -0.3 85,612 - -0.9 85,637 + -0.2 85,638 - -1.4 85,648 + -0.7 85,689 + -0.9 85,689 + -1.2 85,689 + -0.9 85,689 + +0.9 85,689 + -0.7 85,690 - -1.3 85,704 + -0.3 85,704 + -1.5 85,705 - +0.6 85,705 - -0.4 85,705 - -0.2 85,705 - -0.1 85,705 - -1.2 85,706 + -0.8 85,708 + -0.9 85,708 + +0.3 85,708 + -0.5 85,708 + -0.1 85,708 + +0.1 85,708 + -0.7 85,708 + -0.8 85,708 + -2.0 85,708 + +0.2 85,708 + -1.4 85,708 + -0.1 85,708 + +0.1 85,708 + +0.5 85,708 + -0.5 85,708 + +0.8 85,708 + +0.2 85,708 + -1.1 85,708 + -0.9 85,708 + -0.4 85,708 + -1.0 85,708 + +1.3 85,708 + +0.3 85,708 + +1.0 85,709 - +0.6 85,709 - +0.2 85,709 - -0.5 85,709 - +0.0 85,709 - -0.6 85,709 - -1.7 85,709 - +0.2 85,709 - -0.1 85,709 - -1.0 85,709 - +0.1 85,709 - -0.4 85,709 - -0.5 85,709 - -0.5 85,710 - -0.2 85,721 + -0.6 85,721 + +0.7 85,721 + -2.2 85,721 + +0.0 85,721 + -2.1 85,721 + -1.6 85,721 + -0.5 85,721 + +0.5 85,721 + +0.0 85,722 - +0.9 85,722 - +0.0 85,722 - -0.4 85,722 - +0.1 85,722 - -0.4 85,722 - -1.1 85,722 - -0.4 85,722 - +0.3 85,722 - -0.7 85,722 - +0.2 85,722 - -0.5 85,722 - +0.5
Or see this region's nucleotide sequence