Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3872 and GFF3873 overlap by 4 nucleotides GFF3873 and GFF3874 are separated by 13 nucleotides GFF3874 and GFF3875 overlap by 4 nucleotides
GFF3872 - DNA recombination and repair protein RecO, at 70,306 to 71,076
GFF3872
GFF3873 - Pyridoxine 5'-phosphate synthase (EC 2.6.99.2), at 71,073 to 71,834
GFF3873
GFF3874 - Holo-[acyl-carrier-protein] synthase (EC 2.7.8.7), at 71,848 to 72,240
GFF3874
GFF3875 - beta-N-acetylglucosaminidase (EC 3.2.1.52), at 72,237 to 73,319
GFF3875
Position (kb) 71
72
73 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 71.014 kb on + strand at 71.014 kb on + strand at 71.014 kb on + strand at 71.014 kb on + strand at 71.014 kb on + strand at 71.014 kb on + strand at 71.014 kb on + strand at 71.015 kb on - strand at 71.015 kb on - strand at 71.015 kb on - strand at 71.015 kb on - strand at 71.015 kb on - strand at 71.015 kb on - strand at 71.015 kb on - strand at 71.015 kb on - strand at 71.015 kb on - strand at 71.040 kb on + strand at 71.040 kb on + strand at 71.041 kb on - strand at 71.072 kb on + strand at 71.072 kb on + strand at 71.072 kb on + strand at 71.072 kb on + strand at 71.072 kb on + strand at 71.072 kb on + strand at 71.072 kb on + strand at 71.072 kb on + strand at 71.073 kb on - strand at 71.073 kb on - strand at 71.073 kb on - strand at 71.073 kb on - strand at 71.073 kb on - strand at 71.073 kb on - strand at 71.854 kb on + strand at 71.854 kb on + strand at 71.854 kb on + strand at 71.855 kb on - strand at 71.856 kb on - strand at 72.004 kb on + strand, within GFF3874 at 72.004 kb on + strand, within GFF3874 at 72.005 kb on - strand, within GFF3874 at 72.005 kb on - strand, within GFF3874 at 72.205 kb on + strand at 72.235 kb on + strand at 72.236 kb on + strand at 72.236 kb on + strand at 72.236 kb on + strand at 72.236 kb on + strand at 72.236 kb on + strand at 72.236 kb on + strand at 72.236 kb on + strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.237 kb on - strand at 72.861 kb on + strand, within GFF3875 at 72.861 kb on + strand, within GFF3875 at 72.861 kb on + strand, within GFF3875 at 72.862 kb on - strand, within GFF3875 at 72.912 kb on + strand, within GFF3875 at 72.913 kb on - strand, within GFF3875 at 73.035 kb on + strand, within GFF3875 at 73.036 kb on - strand, within GFF3875 at 73.036 kb on - strand, within GFF3875 at 73.036 kb on - strand, within GFF3875 at 73.036 kb on - strand, within GFF3875 at 73.036 kb on - strand, within GFF3875 at 73.059 kb on + strand, within GFF3875 at 73.059 kb on + strand, within GFF3875 at 73.060 kb on - strand, within GFF3875
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 71,014 + -0.3 71,014 + -2.9 71,014 + -1.0 71,014 + +0.1 71,014 + -0.3 71,014 + +0.2 71,014 + -0.3 71,015 - -0.7 71,015 - -0.0 71,015 - -1.0 71,015 - -0.2 71,015 - +0.0 71,015 - -1.0 71,015 - +0.0 71,015 - -1.8 71,015 - -0.4 71,040 + -0.5 71,040 + -1.4 71,041 - -0.4 71,072 + -0.6 71,072 + -3.1 71,072 + -0.7 71,072 + -0.9 71,072 + +1.8 71,072 + -0.7 71,072 + -0.3 71,072 + -0.1 71,073 - -2.2 71,073 - -0.4 71,073 - +0.2 71,073 - -0.0 71,073 - +0.2 71,073 - -1.1 71,854 + +0.2 71,854 + -0.3 71,854 + +0.1 71,855 - +0.6 71,856 - +0.0 72,004 + GFF3874 0.40 -0.2 72,004 + GFF3874 0.40 -1.3 72,005 - GFF3874 0.40 -0.6 72,005 - GFF3874 0.40 -0.7 72,205 + +0.8 72,235 + +0.5 72,236 + +0.2 72,236 + -0.4 72,236 + -0.2 72,236 + -0.8 72,236 + +0.7 72,236 + +0.5 72,236 + +0.4 72,237 - +0.9 72,237 - +0.2 72,237 - +0.9 72,237 - +1.0 72,237 - +0.1 72,237 - -0.3 72,237 - -0.2 72,237 - +0.3 72,237 - -0.1 72,237 - -2.0 72,237 - -1.0 72,237 - -2.7 72,237 - -0.5 72,861 + GFF3875 0.58 -1.2 72,861 + GFF3875 0.58 -0.6 72,861 + GFF3875 0.58 +0.1 72,862 - GFF3875 0.58 -0.4 72,912 + GFF3875 0.62 -0.0 72,913 - GFF3875 0.62 +1.1 73,035 + GFF3875 0.74 -0.8 73,036 - GFF3875 0.74 -0.3 73,036 - GFF3875 0.74 +0.7 73,036 - GFF3875 0.74 -0.2 73,036 - GFF3875 0.74 -1.3 73,036 - GFF3875 0.74 -1.7 73,059 + GFF3875 0.76 +2.2 73,059 + GFF3875 0.76 -0.4 73,060 - GFF3875 0.76 -0.8
Or see this region's nucleotide sequence