Strain Fitness in Variovorax sp. SCN45 around GFF3821

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
6,665	-		GFF3820	0.67	-1.1
6,665	-		GFF3820	0.67	-0.9
6,665	-		GFF3820	0.67	+0.3
6,665	-		GFF3820	0.67	-0.7
6,721	+		GFF3820	0.71	-1.7
6,722	-		GFF3820	0.71	-0.0
6,749	-		GFF3820	0.73	+0.9
6,749	-		GFF3820	0.73	-0.5
6,749	-		GFF3820	0.73	-0.3
6,862	+		GFF3820	0.80	+0.1
7,051	+				-1.0
7,051	+				+0.9
7,195	+				-1.0
7,195	+				-0.7
7,196	-				-0.3
7,196	-				-0.6
7,533	+		GFF3821	0.12	+1.2
7,534	-		GFF3821	0.12	-0.2
7,674	-		GFF3821	0.17	+1.0
7,680	+		GFF3821	0.17	-0.1
7,680	+		GFF3821	0.17	-0.8
7,680	+		GFF3821	0.17	+0.4
7,681	-		GFF3821	0.17	+0.6
7,681	-		GFF3821	0.17	+0.4
7,741	-		GFF3821	0.19	+0.8
7,836	+		GFF3821	0.22	+0.8
7,837	-		GFF3821	0.22	-0.6
7,854	+		GFF3821	0.22	-0.3
7,878	+		GFF3821	0.23	+0.4
7,878	+		GFF3821	0.23	-1.5
7,878	+		GFF3821	0.23	-0.4
7,878	+		GFF3821	0.23	-0.4
7,878	+		GFF3821	0.23	+0.7
7,878	+		GFF3821	0.23	-0.2
7,879	-		GFF3821	0.23	-0.2
7,995	+		GFF3821	0.27	+0.2
7,995	+		GFF3821	0.27	-2.1
7,995	+		GFF3821	0.27	-0.7
7,995	+		GFF3821	0.27	-0.1
7,995	+		GFF3821	0.27	+0.1
7,995	+		GFF3821	0.27	-2.3
7,995	+		GFF3821	0.27	+1.0
7,996	-		GFF3821	0.27	+0.0
7,996	-		GFF3821	0.27	-0.5
7,996	-		GFF3821	0.27	+0.1
7,996	-		GFF3821	0.27	-0.8
7,996	-		GFF3821	0.27	+1.0
8,037	+		GFF3821	0.28	+0.7
8,037	+		GFF3821	0.28	-1.3
8,037	+		GFF3821	0.28	-0.1
8,037	+		GFF3821	0.28	-0.2
8,037	+		GFF3821	0.28	+0.1
8,038	-		GFF3821	0.28	+1.6
8,038	-		GFF3821	0.28	+0.9
8,038	-		GFF3821	0.28	-0.9
8,038	-		GFF3821	0.28	-0.7
8,502	+		GFF3821	0.43	+1.2
8,502	+		GFF3821	0.43	+0.3
8,502	+		GFF3821	0.43	-1.0
8,502	+		GFF3821	0.43	+0.1
8,502	+		GFF3821	0.43	+0.5
8,503	-		GFF3821	0.43	-0.4
8,503	-		GFF3821	0.43	+0.4
8,503	-		GFF3821	0.43	+0.3
8,658	+		GFF3821	0.48	-0.0
8,658	+		GFF3821	0.48	-0.6
8,658	+		GFF3821	0.48	+0.2
8,658	+		GFF3821	0.48	+0.4
8,658	+		GFF3821	0.48	-0.4
8,659	-		GFF3821	0.48	-0.1
8,659	-		GFF3821	0.48	+0.3
8,659	-		GFF3821	0.48	-0.1
8,848	+		GFF3821	0.54	-0.1
8,913	+		GFF3821	0.56	-1.4
8,913	+		GFF3821	0.56	-0.6
9,150	+		GFF3821	0.63	+0.6
9,246	+		GFF3821	0.66	-0.0
9,246	+		GFF3821	0.66	-0.4
9,246	+		GFF3821	0.66	-0.2
9,246	+		GFF3821	0.66	-0.1
9,246	+		GFF3821	0.66	+0.4
9,246	+		GFF3821	0.66	-1.0
9,246	+		GFF3821	0.66	+1.4
9,246	+		GFF3821	0.66	+0.2
9,246	+		GFF3821	0.66	-0.7
9,246	+		GFF3821	0.66	+0.7
9,247	-		GFF3821	0.66	-0.4
9,247	-		GFF3821	0.66	-0.7
9,247	-		GFF3821	0.66	+0.3
9,247	-		GFF3821	0.66	-1.2
9,247	-		GFF3821	0.66	+0.2
9,247	-		GFF3821	0.66	+0.3
9,247	-		GFF3821	0.66	-0.6
9,247	-		GFF3821	0.66	+0.6
9,247	-		GFF3821	0.66	-0.6
9,333	+		GFF3821	0.69	-0.3
9,333	+		GFF3821	0.69	-0.3
9,334	-		GFF3821	0.69	+0.0
9,334	-		GFF3821	0.69	-1.5
9,726	+		GFF3821	0.81	+0.2
9,726	+		GFF3821	0.81	+0.2
9,726	+		GFF3821	0.81	+0.6
9,727	-		GFF3821	0.82	-0.4
9,727	-		GFF3821	0.82	+0.7
9,769	-		GFF3821	0.83	+0.2
9,769	-		GFF3821	0.83	-1.6
9,843	+		GFF3821	0.85	+0.2
9,843	+		GFF3821	0.85	+0.5
9,843	+		GFF3821	0.85	+0.2
9,843	+		GFF3821	0.85	+0.0
9,844	-		GFF3821	0.85	-0.1
9,844	-		GFF3821	0.85	+0.3
9,844	-		GFF3821	0.85	-0.4
9,844	-		GFF3821	0.85	+0.2
9,844	-		GFF3821	0.85	+0.5
9,844	-		GFF3821	0.85	+0.9
9,844	-		GFF3821	0.85	+0.1
9,885	+		GFF3821	0.87	-0.1
9,885	+		GFF3821	0.87	+0.5
9,885	+		GFF3821	0.87	-0.4
9,886	-		GFF3821	0.87	-0.3
9,886	-		GFF3821	0.87	-0.4
9,886	-		GFF3821	0.87	+0.0
9,971	-		GFF3821	0.89	-0.5
9,971	-		GFF3821	0.89	+0.4
9,971	-		GFF3821	0.89	-1.3
9,971	-		GFF3821	0.89	-1.1
9,971	-		GFF3821	0.89	-0.2
9,971	-		GFF3821	0.89	-0.7
10,083	+				-0.6
10,083	+				-0.3
10,083	+				-0.6
10,083	+				-1.0
10,083	+				-1.7
10,083	+				+0.4
10,083	+				-0.1
10,084	-				-0.7
10,084	-				+0.2
10,084	-				+0.6
10,084	-				-0.5
10,084	-				+0.8
10,084	-				+0.6
10,084	-				-0.1
10,084	-				-0.9
10,084	-				+1.3
10,084	-				-0.0
10,167	+				-0.7
10,167	+				-1.4
10,209	+				-0.8
10,210	-				+1.6
10,296	-				-0.6
10,430	+				+0.4
10,430	+				-0.7
10,431	-				-0.9
10,431	-				+0.0
10,431	-				-1.2
10,431	-				+0.3
10,431	-				-0.3
10,674	+		GFF3822	0.28	-0.1
10,674	+		GFF3822	0.28	-0.1
10,674	+		GFF3822	0.28	-0.7
10,674	+		GFF3822	0.28	-0.6
10,674	+		GFF3822	0.28	-2.2
10,674	+		GFF3822	0.28	+0.3
10,674	+		GFF3822	0.28	-0.6
10,675	-		GFF3822	0.28	-0.4
10,675	-		GFF3822	0.28	+0.6
10,675	-		GFF3822	0.28	+0.5
10,675	-		GFF3822	0.28	+0.0
10,675	-		GFF3822	0.28	-0.1
10,675	-		GFF3822	0.28	+1.0
10,788	+		GFF3822	0.37	+1.0
10,788	+		GFF3822	0.37	+1.0
10,788	+		GFF3822	0.37	-0.2
10,788	+		GFF3822	0.37	-0.5
10,789	-		GFF3822	0.38	+0.9
10,817	+		GFF3822	0.40	-0.9
10,817	+		GFF3822	0.40	+0.3
10,817	+		GFF3822	0.40	+0.1
10,817	+		GFF3822	0.40	-0.7
10,817	+		GFF3822	0.40	-0.4
10,817	+		GFF3822	0.40	-0.5
10,818	-		GFF3822	0.40	-0.3
10,818	-		GFF3822	0.40	+0.6
10,818	-		GFF3822	0.40	+0.5
11,035	-		GFF3822	0.57	+0.4
11,035	-		GFF3822	0.57	-0.2
11,271	+		GFF3822	0.76	+1.1
11,271	+		GFF3822	0.76	-0.5
11,272	-		GFF3822	0.76	-1.4