Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3763 and GFF3764 are separated by 6 nucleotides GFF3764 and GFF3765 overlap by 4 nucleotides GFF3765 and GFF3766 overlap by 11 nucleotides GFF3766 and GFF3767 overlap by 14 nucleotides
GFF3763 - no description, at 255,497 to 256,015
GFF3763
GFF3764 - INTEGRAL MEMBRANE PROTEIN (Rhomboid family), at 256,022 to 256,753
GFF3764
GFF3765 - no description, at 256,750 to 257,751
GFF3765
GFF3766 - hypothetical protein, at 257,741 to 258,472
GFF3766
GFF3767 - 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases, at 258,459 to 259,235
GFF3767
Position (kb)
256
257
258 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 255.752 kb on + strand, within GFF3763 at 255.752 kb on + strand, within GFF3763 at 255.752 kb on + strand, within GFF3763 at 255.752 kb on + strand, within GFF3763 at 255.752 kb on + strand, within GFF3763 at 255.752 kb on + strand, within GFF3763 at 255.752 kb on + strand, within GFF3763 at 255.753 kb on - strand, within GFF3763 at 255.753 kb on - strand, within GFF3763 at 255.753 kb on - strand, within GFF3763 at 255.753 kb on - strand, within GFF3763 at 255.753 kb on - strand, within GFF3763 at 255.753 kb on - strand, within GFF3763 at 255.753 kb on - strand, within GFF3763 at 255.770 kb on + strand, within GFF3763 at 255.770 kb on + strand, within GFF3763 at 255.770 kb on + strand, within GFF3763 at 255.771 kb on - strand, within GFF3763 at 255.771 kb on - strand, within GFF3763 at 255.771 kb on - strand, within GFF3763 at 255.771 kb on - strand, within GFF3763 at 256.040 kb on + strand at 256.040 kb on + strand at 256.040 kb on + strand at 256.040 kb on + strand at 256.041 kb on - strand at 256.041 kb on - strand at 256.041 kb on - strand at 256.041 kb on - strand at 256.041 kb on - strand at 256.112 kb on + strand, within GFF3764 at 256.112 kb on + strand, within GFF3764 at 256.112 kb on + strand, within GFF3764 at 256.113 kb on - strand, within GFF3764 at 256.211 kb on + strand, within GFF3764 at 256.212 kb on - strand, within GFF3764 at 256.212 kb on - strand, within GFF3764 at 256.212 kb on - strand, within GFF3764 at 256.212 kb on - strand, within GFF3764 at 256.212 kb on - strand, within GFF3764 at 256.232 kb on + strand, within GFF3764 at 256.232 kb on + strand, within GFF3764 at 256.233 kb on - strand, within GFF3764 at 256.233 kb on - strand, within GFF3764 at 256.233 kb on - strand, within GFF3764 at 256.233 kb on - strand, within GFF3764 at 256.233 kb on - strand, within GFF3764 at 256.777 kb on + strand at 256.777 kb on + strand at 256.864 kb on + strand, within GFF3765 at 256.909 kb on + strand, within GFF3765 at 256.969 kb on + strand, within GFF3765 at 256.969 kb on + strand, within GFF3765 at 256.969 kb on + strand, within GFF3765 at 256.969 kb on + strand, within GFF3765 at 256.969 kb on + strand, within GFF3765 at 256.969 kb on + strand, within GFF3765 at 256.969 kb on + strand, within GFF3765 at 256.970 kb on - strand, within GFF3765 at 256.985 kb on - strand, within GFF3765 at 257.053 kb on + strand, within GFF3765 at 257.053 kb on + strand, within GFF3765 at 257.053 kb on + strand, within GFF3765 at 257.053 kb on + strand, within GFF3765 at 257.054 kb on - strand, within GFF3765 at 257.054 kb on - strand, within GFF3765 at 257.179 kb on + strand, within GFF3765 at 257.179 kb on + strand, within GFF3765 at 257.179 kb on + strand, within GFF3765 at 257.180 kb on - strand, within GFF3765 at 257.180 kb on - strand, within GFF3765 at 257.180 kb on - strand, within GFF3765 at 257.180 kb on - strand, within GFF3765 at 257.180 kb on - strand, within GFF3765 at 257.180 kb on - strand, within GFF3765 at 257.248 kb on + strand, within GFF3765 at 257.248 kb on + strand, within GFF3765 at 257.248 kb on + strand, within GFF3765 at 257.248 kb on + strand, within GFF3765 at 257.248 kb on + strand, within GFF3765 at 257.249 kb on - strand, within GFF3765 at 257.249 kb on - strand, within GFF3765 at 257.249 kb on - strand, within GFF3765 at 257.365 kb on + strand, within GFF3765 at 257.366 kb on - strand, within GFF3765 at 257.476 kb on + strand, within GFF3765 at 257.554 kb on + strand, within GFF3765 at 257.555 kb on - strand, within GFF3765 at 257.555 kb on - strand, within GFF3765 at 257.592 kb on - strand, within GFF3765 at 257.638 kb on + strand, within GFF3765 at 257.639 kb on - strand, within GFF3765 at 257.677 kb on + strand at 257.678 kb on - strand at 257.678 kb on - strand at 257.678 kb on - strand at 257.678 kb on - strand at 257.678 kb on - strand at 257.678 kb on - strand at 257.678 kb on - strand at 257.861 kb on + strand, within GFF3766 at 257.861 kb on + strand, within GFF3766 at 257.861 kb on + strand, within GFF3766 at 257.861 kb on + strand, within GFF3766 at 257.861 kb on + strand, within GFF3766 at 257.861 kb on + strand, within GFF3766 at 257.861 kb on + strand, within GFF3766 at 257.862 kb on - strand, within GFF3766 at 257.862 kb on - strand, within GFF3766 at 257.862 kb on - strand, within GFF3766 at 257.862 kb on - strand, within GFF3766 at 257.862 kb on - strand, within GFF3766 at 257.862 kb on - strand, within GFF3766 at 257.862 kb on - strand, within GFF3766 at 258.218 kb on + strand, within GFF3766 at 258.347 kb on + strand, within GFF3766 at 258.348 kb on - strand, within GFF3766 at 258.348 kb on - strand, within GFF3766 at 258.348 kb on - strand, within GFF3766 at 258.348 kb on - strand, within GFF3766 at 258.380 kb on + strand, within GFF3766 at 258.381 kb on - strand, within GFF3766 at 258.381 kb on - strand, within GFF3766 at 258.404 kb on + strand at 258.404 kb on + strand at 258.404 kb on + strand at 258.404 kb on + strand at 258.404 kb on + strand at 258.404 kb on + strand at 258.404 kb on + strand at 258.404 kb on + strand at 258.405 kb on - strand at 258.405 kb on - strand at 258.405 kb on - strand at 258.405 kb on - strand at 258.405 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 255,752 + GFF3763 0.49 +0.8 255,752 + GFF3763 0.49 -0.8 255,752 + GFF3763 0.49 -1.2 255,752 + GFF3763 0.49 -0.6 255,752 + GFF3763 0.49 -1.3 255,752 + GFF3763 0.49 -0.1 255,752 + GFF3763 0.49 +0.8 255,753 - GFF3763 0.49 -0.1 255,753 - GFF3763 0.49 -0.2 255,753 - GFF3763 0.49 -0.1 255,753 - GFF3763 0.49 +0.4 255,753 - GFF3763 0.49 -0.2 255,753 - GFF3763 0.49 +1.8 255,753 - GFF3763 0.49 -1.4 255,770 + GFF3763 0.53 -1.4 255,770 + GFF3763 0.53 -0.3 255,770 + GFF3763 0.53 +0.1 255,771 - GFF3763 0.53 -0.7 255,771 - GFF3763 0.53 +0.8 255,771 - GFF3763 0.53 +0.6 255,771 - GFF3763 0.53 -0.7 256,040 + +0.3 256,040 + +0.9 256,040 + +0.1 256,040 + +1.8 256,041 - -0.4 256,041 - +0.4 256,041 - +0.4 256,041 - -0.6 256,041 - +1.1 256,112 + GFF3764 0.12 -1.1 256,112 + GFF3764 0.12 +0.2 256,112 + GFF3764 0.12 -0.1 256,113 - GFF3764 0.12 +0.4 256,211 + GFF3764 0.26 +1.5 256,212 - GFF3764 0.26 +0.2 256,212 - GFF3764 0.26 -0.9 256,212 - GFF3764 0.26 +1.6 256,212 - GFF3764 0.26 +0.0 256,212 - GFF3764 0.26 -0.8 256,232 + GFF3764 0.29 +0.8 256,232 + GFF3764 0.29 -0.2 256,233 - GFF3764 0.29 -0.5 256,233 - GFF3764 0.29 -0.7 256,233 - GFF3764 0.29 -1.1 256,233 - GFF3764 0.29 -0.6 256,233 - GFF3764 0.29 -0.2 256,777 + -0.6 256,777 + +1.5 256,864 + GFF3765 0.11 +0.5 256,909 + GFF3765 0.16 -0.2 256,969 + GFF3765 0.22 -0.4 256,969 + GFF3765 0.22 +0.6 256,969 + GFF3765 0.22 +0.2 256,969 + GFF3765 0.22 +0.2 256,969 + GFF3765 0.22 +0.1 256,969 + GFF3765 0.22 -0.2 256,969 + GFF3765 0.22 +0.6 256,970 - GFF3765 0.22 +1.2 256,985 - GFF3765 0.23 +0.7 257,053 + GFF3765 0.30 +0.4 257,053 + GFF3765 0.30 -0.3 257,053 + GFF3765 0.30 +0.9 257,053 + GFF3765 0.30 -0.2 257,054 - GFF3765 0.30 -0.9 257,054 - GFF3765 0.30 +0.4 257,179 + GFF3765 0.43 +0.2 257,179 + GFF3765 0.43 -0.5 257,179 + GFF3765 0.43 -0.1 257,180 - GFF3765 0.43 -0.5 257,180 - GFF3765 0.43 -0.7 257,180 - GFF3765 0.43 -1.0 257,180 - GFF3765 0.43 -0.8 257,180 - GFF3765 0.43 -0.3 257,180 - GFF3765 0.43 +0.8 257,248 + GFF3765 0.50 +0.4 257,248 + GFF3765 0.50 +1.0 257,248 + GFF3765 0.50 +0.6 257,248 + GFF3765 0.50 +0.5 257,248 + GFF3765 0.50 +1.3 257,249 - GFF3765 0.50 -0.3 257,249 - GFF3765 0.50 -1.0 257,249 - GFF3765 0.50 -0.8 257,365 + GFF3765 0.61 -0.3 257,366 - GFF3765 0.61 -0.5 257,476 + GFF3765 0.72 +0.8 257,554 + GFF3765 0.80 +0.3 257,555 - GFF3765 0.80 +0.1 257,555 - GFF3765 0.80 -1.2 257,592 - GFF3765 0.84 -0.5 257,638 + GFF3765 0.89 -2.0 257,639 - GFF3765 0.89 +1.2 257,677 + -0.1 257,678 - +0.4 257,678 - +0.2 257,678 - -1.0 257,678 - -0.3 257,678 - -1.9 257,678 - +0.6 257,678 - +0.8 257,861 + GFF3766 0.16 +0.1 257,861 + GFF3766 0.16 -0.5 257,861 + GFF3766 0.16 -0.9 257,861 + GFF3766 0.16 -0.1 257,861 + GFF3766 0.16 +0.0 257,861 + GFF3766 0.16 -0.3 257,861 + GFF3766 0.16 +0.2 257,862 - GFF3766 0.17 -0.7 257,862 - GFF3766 0.17 +0.0 257,862 - GFF3766 0.17 +1.0 257,862 - GFF3766 0.17 -0.4 257,862 - GFF3766 0.17 +0.4 257,862 - GFF3766 0.17 -0.1 257,862 - GFF3766 0.17 +1.2 258,218 + GFF3766 0.65 -1.6 258,347 + GFF3766 0.83 -0.1 258,348 - GFF3766 0.83 +0.5 258,348 - GFF3766 0.83 -0.1 258,348 - GFF3766 0.83 -0.7 258,348 - GFF3766 0.83 -1.1 258,380 + GFF3766 0.87 -0.7 258,381 - GFF3766 0.87 +0.6 258,381 - GFF3766 0.87 -0.2 258,404 + -1.2 258,404 + +0.0 258,404 + -0.2 258,404 + -0.5 258,404 + +1.5 258,404 + -0.3 258,404 + +0.7 258,404 + -0.2 258,405 - -0.2 258,405 - -0.4 258,405 - +0.2 258,405 - -0.8 258,405 - -0.1
Or see this region's nucleotide sequence