Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3737 and GFF3738 overlap by 4 nucleotides GFF3738 and GFF3739 are separated by 29 nucleotides GFF3739 and GFF3740 are separated by 87 nucleotides GFF3740 and GFF3741 overlap by 4 nucleotides
GFF3737 - DnaA initiator-associating protein DiaA, at 233,577 to 234,221
GFF3737
GFF3738 - Uncharacterized protein YraP, at 234,218 to 235,030
GFF3738
GFF3739 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 235,060 to 235,968
GFF3739
GFF3740 - Transcriptional regulator, MerR family, at 236,056 to 236,538
GFF3740
GFF3741 - Uncharacterized MFS-type transporter, at 236,535 to 238,085
GFF3741
Position (kb)
235
236 Strain fitness (log2 ratio)
-2
-1
0
1 at 234.194 kb on - strand at 234.194 kb on - strand at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.473 kb on + strand, within GFF3738 at 234.473 kb on + strand, within GFF3738 at 234.473 kb on + strand, within GFF3738 at 234.473 kb on + strand, within GFF3738 at 234.474 kb on - strand, within GFF3738 at 234.474 kb on - strand, within GFF3738 at 234.474 kb on - strand, within GFF3738 at 234.474 kb on - strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.629 kb on + strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.630 kb on - strand, within GFF3738 at 234.719 kb on + strand, within GFF3738 at 234.720 kb on - strand, within GFF3738 at 234.720 kb on - strand, within GFF3738 at 235.061 kb on + strand at 235.127 kb on + strand at 235.128 kb on - strand at 235.841 kb on + strand, within GFF3739 at 235.841 kb on + strand, within GFF3739 at 235.842 kb on - strand, within GFF3739 at 235.842 kb on - strand, within GFF3739 at 235.842 kb on - strand, within GFF3739 at 235.842 kb on - strand, within GFF3739 at 235.944 kb on - strand at 235.981 kb on + strand at 235.982 kb on - strand at 235.982 kb on - strand at 236.029 kb on + strand at 236.137 kb on + strand, within GFF3740 at 236.137 kb on + strand, within GFF3740 at 236.137 kb on + strand, within GFF3740 at 236.137 kb on + strand, within GFF3740 at 236.137 kb on + strand, within GFF3740 at 236.138 kb on - strand, within GFF3740 at 236.138 kb on - strand, within GFF3740 at 236.138 kb on - strand, within GFF3740 at 236.138 kb on - strand, within GFF3740 at 236.141 kb on - strand, within GFF3740 at 236.141 kb on - strand, within GFF3740 at 236.194 kb on + strand, within GFF3740 at 236.195 kb on - strand, within GFF3740 at 236.195 kb on - strand, within GFF3740 at 236.195 kb on - strand, within GFF3740 at 236.461 kb on + strand, within GFF3740 at 236.461 kb on + strand, within GFF3740 at 236.461 kb on + strand, within GFF3740 at 236.461 kb on + strand, within GFF3740 at 236.625 kb on + strand at 236.661 kb on + strand at 236.661 kb on + strand at 236.662 kb on - strand at 236.662 kb on - strand at 236.662 kb on - strand at 236.718 kb on + strand, within GFF3741 at 236.718 kb on + strand, within GFF3741 at 236.719 kb on - strand, within GFF3741 at 236.779 kb on - strand, within GFF3741 at 236.788 kb on - strand, within GFF3741 at 236.886 kb on + strand, within GFF3741 at 236.887 kb on - strand, within GFF3741
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 234,194 - -0.4 234,194 - +1.2 234,455 + GFF3738 0.29 +0.9 234,455 + GFF3738 0.29 -1.5 234,455 + GFF3738 0.29 +0.2 234,455 + GFF3738 0.29 -1.0 234,455 + GFF3738 0.29 +0.4 234,455 + GFF3738 0.29 -0.7 234,455 + GFF3738 0.29 +0.7 234,455 + GFF3738 0.29 -0.4 234,455 + GFF3738 0.29 -0.1 234,455 + GFF3738 0.29 -0.9 234,455 + GFF3738 0.29 -0.1 234,455 + GFF3738 0.29 -0.5 234,456 - GFF3738 0.29 -0.3 234,456 - GFF3738 0.29 -1.1 234,456 - GFF3738 0.29 +1.1 234,456 - GFF3738 0.29 -0.6 234,456 - GFF3738 0.29 -0.8 234,456 - GFF3738 0.29 +0.2 234,456 - GFF3738 0.29 -0.1 234,456 - GFF3738 0.29 -0.2 234,456 - GFF3738 0.29 -0.6 234,456 - GFF3738 0.29 -0.8 234,456 - GFF3738 0.29 +0.1 234,456 - GFF3738 0.29 -0.1 234,473 + GFF3738 0.31 +1.1 234,473 + GFF3738 0.31 -1.6 234,473 + GFF3738 0.31 +0.0 234,473 + GFF3738 0.31 -1.0 234,474 - GFF3738 0.31 -1.1 234,474 - GFF3738 0.31 -0.1 234,474 - GFF3738 0.31 -0.6 234,474 - GFF3738 0.31 +0.4 234,629 + GFF3738 0.51 -0.4 234,629 + GFF3738 0.51 -1.6 234,629 + GFF3738 0.51 -0.1 234,629 + GFF3738 0.51 -1.9 234,629 + GFF3738 0.51 -0.6 234,629 + GFF3738 0.51 -1.0 234,629 + GFF3738 0.51 -0.5 234,629 + GFF3738 0.51 -0.9 234,629 + GFF3738 0.51 -1.3 234,629 + GFF3738 0.51 -0.2 234,629 + GFF3738 0.51 +0.4 234,629 + GFF3738 0.51 +0.6 234,630 - GFF3738 0.51 -0.9 234,630 - GFF3738 0.51 -1.9 234,630 - GFF3738 0.51 -0.6 234,630 - GFF3738 0.51 -0.3 234,630 - GFF3738 0.51 -0.2 234,630 - GFF3738 0.51 -0.4 234,630 - GFF3738 0.51 +0.1 234,630 - GFF3738 0.51 +0.3 234,630 - GFF3738 0.51 -0.7 234,630 - GFF3738 0.51 -0.5 234,630 - GFF3738 0.51 -1.0 234,630 - GFF3738 0.51 -0.4 234,630 - GFF3738 0.51 -0.3 234,630 - GFF3738 0.51 +0.5 234,630 - GFF3738 0.51 -0.1 234,630 - GFF3738 0.51 -0.2 234,719 + GFF3738 0.62 +0.1 234,720 - GFF3738 0.62 -1.2 234,720 - GFF3738 0.62 -0.4 235,061 + -0.8 235,127 + -0.3 235,128 - -0.0 235,841 + GFF3739 0.86 -0.6 235,841 + GFF3739 0.86 -0.3 235,842 - GFF3739 0.86 -0.5 235,842 - GFF3739 0.86 -1.5 235,842 - GFF3739 0.86 -1.0 235,842 - GFF3739 0.86 -0.4 235,944 - -0.1 235,981 + -0.8 235,982 - +0.8 235,982 - +0.2 236,029 + -0.3 236,137 + GFF3740 0.17 -0.2 236,137 + GFF3740 0.17 +0.0 236,137 + GFF3740 0.17 -0.7 236,137 + GFF3740 0.17 +0.8 236,137 + GFF3740 0.17 -0.1 236,138 - GFF3740 0.17 -0.7 236,138 - GFF3740 0.17 -0.2 236,138 - GFF3740 0.17 +0.0 236,138 - GFF3740 0.17 -0.4 236,141 - GFF3740 0.18 +0.0 236,141 - GFF3740 0.18 -0.1 236,194 + GFF3740 0.29 -0.4 236,195 - GFF3740 0.29 -0.4 236,195 - GFF3740 0.29 +0.2 236,195 - GFF3740 0.29 +0.3 236,461 + GFF3740 0.84 -0.0 236,461 + GFF3740 0.84 -0.6 236,461 + GFF3740 0.84 +0.1 236,461 + GFF3740 0.84 -0.0 236,625 + +0.2 236,661 + -0.3 236,661 + +0.1 236,662 - -0.7 236,662 - -1.4 236,662 - -1.1 236,718 + GFF3741 0.12 -0.8 236,718 + GFF3741 0.12 +1.2 236,719 - GFF3741 0.12 -0.2 236,779 - GFF3741 0.16 -0.8 236,788 - GFF3741 0.16 -0.6 236,886 + GFF3741 0.23 -0.3 236,887 - GFF3741 0.23 -0.5
Or see this region's nucleotide sequence