Strain Fitness in Variovorax sp. SCN45 around GFF3718

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3716 and GFF3717 are separated by 8 nucleotidesGFF3717 and GFF3718 are separated by 20 nucleotidesGFF3718 and GFF3719 are separated by 14 nucleotides GFF3716 - Possible hydrolase or acyltransferase RutD in novel pyrimidine catabolism pathway, at 214,142 to 214,948 GFF3716 GFF3717 - Putative aminoacrylate peracid reductase RutC @ RidA/YER057c/UK114 superfamily protein, at 214,957 to 215,349 GFF3717 GFF3718 - Peroxyureidoacrylate/ureidoacrylate amidohydrolase RutB (EC 3.5.1.110), at 215,370 to 216,161 GFF3718 GFF3719 - Pyrimidine monooxygenase RutA (EC 1.14.99.46), at 216,176 to 217,288 GFF3719 Position (kb) 215 216 217Strain fitness (log2 ratio) -2 -1 0 1at 214.522 kb on - strand, within GFF3716at 214.758 kb on + strand, within GFF3716at 214.759 kb on - strand, within GFF3716at 214.759 kb on - strand, within GFF3716at 214.759 kb on - strand, within GFF3716at 215.037 kb on - strand, within GFF3717at 215.264 kb on + strand, within GFF3717at 215.264 kb on + strand, within GFF3717at 215.264 kb on + strand, within GFF3717at 215.264 kb on + strand, within GFF3717at 215.265 kb on - strand, within GFF3717at 215.265 kb on - strand, within GFF3717at 215.265 kb on - strand, within GFF3717at 215.297 kb on + strand, within GFF3717at 215.297 kb on + strand, within GFF3717at 215.298 kb on - strand, within GFF3717at 215.479 kb on + strand, within GFF3718at 215.480 kb on - strand, within GFF3718at 215.480 kb on - strand, within GFF3718at 215.549 kb on - strand, within GFF3718at 215.625 kb on + strand, within GFF3718at 215.677 kb on + strand, within GFF3718at 215.678 kb on - strand, within GFF3718at 215.851 kb on + strand, within GFF3718at 215.852 kb on - strand, within GFF3718at 215.852 kb on - strand, within GFF3718at 215.980 kb on + strand, within GFF3718at 215.980 kb on + strand, within GFF3718at 215.981 kb on - strand, within GFF3718at 215.981 kb on - strand, within GFF3718at 215.998 kb on + strand, within GFF3718at 216.366 kb on + strand, within GFF3719at 216.513 kb on + strand, within GFF3719at 216.513 kb on + strand, within GFF3719at 216.513 kb on + strand, within GFF3719at 216.513 kb on + strand, within GFF3719at 216.513 kb on + strand, within GFF3719at 216.513 kb on + strand, within GFF3719at 216.513 kb on + strand, within GFF3719at 216.513 kb on + strand, within GFF3719at 216.513 kb on + strand, within GFF3719at 216.513 kb on + strand, within GFF3719at 216.514 kb on - strand, within GFF3719at 216.514 kb on - strand, within GFF3719at 216.514 kb on - strand, within GFF3719at 216.576 kb on + strand, within GFF3719at 216.576 kb on + strand, within GFF3719at 216.576 kb on + strand, within GFF3719at 216.576 kb on + strand, within GFF3719at 216.576 kb on + strand, within GFF3719at 216.576 kb on + strand, within GFF3719at 216.576 kb on + strand, within GFF3719at 216.577 kb on - strand, within GFF3719at 216.577 kb on - strand, within GFF3719at 216.577 kb on - strand, within GFF3719at 216.577 kb on - strand, within GFF3719at 216.577 kb on - strand, within GFF3719at 216.681 kb on + strand, within GFF3719at 216.681 kb on + strand, within GFF3719at 216.690 kb on + strand, within GFF3719at 216.691 kb on - strand, within GFF3719at 216.850 kb on - strand, within GFF3719at 216.850 kb on - strand, within GFF3719at 216.850 kb on - strand, within GFF3719at 216.850 kb on - strand, within GFF3719at 216.861 kb on + strand, within GFF3719at 216.862 kb on - strand, within GFF3719at 216.862 kb on - strand, within GFF3719at 216.916 kb on - strand, within GFF3719

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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214,522 - GFF3716 0.47 -0.5
214,758 + GFF3716 0.76 -1.0
214,759 - GFF3716 0.76 -0.9
214,759 - GFF3716 0.76 -0.2
214,759 - GFF3716 0.76 +1.2
215,037 - GFF3717 0.20 -0.3
215,264 + GFF3717 0.78 +1.2
215,264 + GFF3717 0.78 +0.4
215,264 + GFF3717 0.78 -0.2
215,264 + GFF3717 0.78 -1.1
215,265 - GFF3717 0.78 +0.4
215,265 - GFF3717 0.78 +0.1
215,265 - GFF3717 0.78 -1.1
215,297 + GFF3717 0.87 -0.6
215,297 + GFF3717 0.87 -1.4
215,298 - GFF3717 0.87 -0.3
215,479 + GFF3718 0.14 -0.6
215,480 - GFF3718 0.14 -0.1
215,480 - GFF3718 0.14 +0.2
215,549 - GFF3718 0.23 -0.8
215,625 + GFF3718 0.32 -0.8
215,677 + GFF3718 0.39 -0.7
215,678 - GFF3718 0.39 -2.5
215,851 + GFF3718 0.61 -0.8
215,852 - GFF3718 0.61 -0.5
215,852 - GFF3718 0.61 +0.8
215,980 + GFF3718 0.77 -0.7
215,980 + GFF3718 0.77 -0.1
215,981 - GFF3718 0.77 +0.8
215,981 - GFF3718 0.77 +0.7
215,998 + GFF3718 0.79 -0.9
216,366 + GFF3719 0.17 -0.1
216,513 + GFF3719 0.30 -1.0
216,513 + GFF3719 0.30 -0.1
216,513 + GFF3719 0.30 -0.5
216,513 + GFF3719 0.30 -0.8
216,513 + GFF3719 0.30 -1.2
216,513 + GFF3719 0.30 -0.6
216,513 + GFF3719 0.30 +0.9
216,513 + GFF3719 0.30 -0.3
216,513 + GFF3719 0.30 -0.1
216,513 + GFF3719 0.30 +0.8
216,514 - GFF3719 0.30 -1.9
216,514 - GFF3719 0.30 -0.1
216,514 - GFF3719 0.30 -1.4
216,576 + GFF3719 0.36 -0.4
216,576 + GFF3719 0.36 +0.0
216,576 + GFF3719 0.36 -0.5
216,576 + GFF3719 0.36 -1.1
216,576 + GFF3719 0.36 -0.1
216,576 + GFF3719 0.36 -0.3
216,576 + GFF3719 0.36 -1.1
216,577 - GFF3719 0.36 -0.2
216,577 - GFF3719 0.36 -0.0
216,577 - GFF3719 0.36 +0.3
216,577 - GFF3719 0.36 -1.4
216,577 - GFF3719 0.36 +0.3
216,681 + GFF3719 0.45 +1.2
216,681 + GFF3719 0.45 +0.4
216,690 + GFF3719 0.46 -0.0
216,691 - GFF3719 0.46 -0.5
216,850 - GFF3719 0.61 -0.2
216,850 - GFF3719 0.61 -1.2
216,850 - GFF3719 0.61 -0.4
216,850 - GFF3719 0.61 -0.3
216,861 + GFF3719 0.62 +0.1
216,862 - GFF3719 0.62 +0.4
216,862 - GFF3719 0.62 +0.1
216,916 - GFF3719 0.66 -1.2

Or see this region's nucleotide sequence