Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3705 and GFF3706 overlap by 4 nucleotides GFF3706 and GFF3707 overlap by 4 nucleotides GFF3707 and GFF3708 are separated by 19 nucleotides
GFF3705 - Amidases related to nicotinamidase, at 205,086 to 205,760
GFF3705
GFF3706 - ABC transporter, permease protein 2 (cluster 11, riboflavin/purine nucleoside/unknown), at 205,757 to 206,689
GFF3706
GFF3707 - ABC transporter, permease protein 1 (cluster 11, riboflavin/purine nucleoside/unknown), at 206,686 to 207,807
GFF3707
GFF3708 - ABC transporter, ATP-binding protein (cluster 11, riboflavin/purine nucleoside/unknown) / ABC transporter, ATP-binding protein (cluster 11, riboflavin/purine nucleoside/unknown), at 207,827 to 209,353
GFF3708
Position (kb) 206
207
208 Strain fitness (log2 ratio) -2
-1
0
1
2 at 205.703 kb on - strand at 205.779 kb on + strand at 205.779 kb on + strand at 205.779 kb on + strand at 205.779 kb on + strand at 205.779 kb on + strand at 205.780 kb on - strand at 205.780 kb on - strand at 205.780 kb on - strand at 205.780 kb on - strand at 205.780 kb on - strand at 205.863 kb on + strand, within GFF3706 at 205.884 kb on + strand, within GFF3706 at 205.885 kb on - strand, within GFF3706 at 206.070 kb on + strand, within GFF3706 at 206.070 kb on + strand, within GFF3706 at 206.071 kb on - strand, within GFF3706 at 206.071 kb on - strand, within GFF3706 at 206.071 kb on - strand, within GFF3706 at 206.071 kb on - strand, within GFF3706 at 206.071 kb on - strand, within GFF3706 at 206.071 kb on - strand, within GFF3706 at 206.657 kb on - strand at 206.774 kb on - strand at 206.774 kb on - strand at 206.774 kb on - strand at 206.805 kb on - strand, within GFF3707 at 206.805 kb on - strand, within GFF3707 at 206.805 kb on - strand, within GFF3707 at 206.930 kb on + strand, within GFF3707 at 206.930 kb on + strand, within GFF3707 at 207.020 kb on + strand, within GFF3707 at 207.020 kb on + strand, within GFF3707 at 207.089 kb on + strand, within GFF3707 at 207.089 kb on + strand, within GFF3707 at 207.254 kb on + strand, within GFF3707 at 207.255 kb on - strand, within GFF3707 at 207.287 kb on + strand, within GFF3707 at 207.287 kb on + strand, within GFF3707 at 207.287 kb on + strand, within GFF3707 at 207.288 kb on - strand, within GFF3707 at 207.288 kb on - strand, within GFF3707 at 207.727 kb on + strand at 207.728 kb on - strand at 207.728 kb on - strand at 207.728 kb on - strand at 208.203 kb on - strand, within GFF3708 at 208.266 kb on + strand, within GFF3708 at 208.266 kb on + strand, within GFF3708 at 208.709 kb on + strand, within GFF3708 at 208.728 kb on + strand, within GFF3708 at 208.728 kb on + strand, within GFF3708
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 205,703 - +0.5 205,779 + +0.1 205,779 + +0.3 205,779 + -0.5 205,779 + -0.8 205,779 + -0.0 205,780 - -0.8 205,780 - -1.0 205,780 - +0.5 205,780 - +1.0 205,780 - +0.1 205,863 + GFF3706 0.11 +0.5 205,884 + GFF3706 0.14 -1.4 205,885 - GFF3706 0.14 +0.5 206,070 + GFF3706 0.34 -0.0 206,070 + GFF3706 0.34 -0.1 206,071 - GFF3706 0.34 -0.2 206,071 - GFF3706 0.34 -1.3 206,071 - GFF3706 0.34 -0.3 206,071 - GFF3706 0.34 -0.4 206,071 - GFF3706 0.34 -2.0 206,071 - GFF3706 0.34 +1.5 206,657 - +2.1 206,774 - +0.3 206,774 - -1.1 206,774 - +0.1 206,805 - GFF3707 0.11 -0.4 206,805 - GFF3707 0.11 -2.5 206,805 - GFF3707 0.11 -1.0 206,930 + GFF3707 0.22 +0.4 206,930 + GFF3707 0.22 -0.6 207,020 + GFF3707 0.30 +0.2 207,020 + GFF3707 0.30 +0.6 207,089 + GFF3707 0.36 +0.1 207,089 + GFF3707 0.36 +0.3 207,254 + GFF3707 0.51 -0.2 207,255 - GFF3707 0.51 -0.2 207,287 + GFF3707 0.54 +0.1 207,287 + GFF3707 0.54 +0.0 207,287 + GFF3707 0.54 -0.5 207,288 - GFF3707 0.54 -1.2 207,288 - GFF3707 0.54 +0.6 207,727 + +0.1 207,728 - -0.6 207,728 - -1.3 207,728 - -0.8 208,203 - GFF3708 0.25 -1.4 208,266 + GFF3708 0.29 -0.5 208,266 + GFF3708 0.29 -1.1 208,709 + GFF3708 0.58 -0.3 208,728 + GFF3708 0.59 -1.1 208,728 + GFF3708 0.59 -0.4
Or see this region's nucleotide sequence