Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3697 and GFF3698 overlap by 4 nucleotides GFF3698 and GFF3699 are separated by 22 nucleotides GFF3699 and GFF3700 are separated by 59 nucleotides
GFF3697 - ATP-dependent DNA ligase (EC 6.5.1.1) LigC, at 196,378 to 198,033
GFF3697
GFF3698 - ATP-dependent, 3'-5' DNA helicase with strand annealing activity, at 198,030 to 200,786
GFF3698
GFF3699 - Thioredoxin 2, at 200,809 to 201,258
GFF3699
GFF3700 - FIG006285: hypothetical protein, at 201,318 to 202,016
GFF3700
Position (kb)
198
199
200
201 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 197.368 kb on + strand, within GFF3697 at 197.369 kb on - strand, within GFF3697 at 197.369 kb on - strand, within GFF3697 at 197.369 kb on - strand, within GFF3697 at 197.584 kb on + strand, within GFF3697 at 197.711 kb on - strand, within GFF3697 at 197.711 kb on - strand, within GFF3697 at 197.711 kb on - strand, within GFF3697 at 197.780 kb on - strand, within GFF3697 at 198.118 kb on + strand at 198.119 kb on - strand at 198.122 kb on + strand at 198.123 kb on - strand at 198.315 kb on + strand, within GFF3698 at 198.315 kb on + strand, within GFF3698 at 198.316 kb on - strand, within GFF3698 at 198.316 kb on - strand, within GFF3698 at 198.316 kb on - strand, within GFF3698 at 198.316 kb on - strand, within GFF3698 at 198.997 kb on - strand, within GFF3698 at 198.997 kb on - strand, within GFF3698 at 199.431 kb on + strand, within GFF3698 at 199.431 kb on + strand, within GFF3698 at 199.432 kb on - strand, within GFF3698 at 199.432 kb on - strand, within GFF3698 at 199.905 kb on + strand, within GFF3698 at 199.905 kb on + strand, within GFF3698 at 199.906 kb on - strand, within GFF3698 at 199.942 kb on + strand, within GFF3698 at 199.942 kb on + strand, within GFF3698 at 199.942 kb on + strand, within GFF3698 at 199.942 kb on + strand, within GFF3698 at 200.136 kb on + strand, within GFF3698 at 200.412 kb on + strand, within GFF3698 at 200.413 kb on - strand, within GFF3698 at 200.413 kb on - strand, within GFF3698 at 201.483 kb on + strand, within GFF3700
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 197,368 + GFF3697 0.60 -1.5 197,369 - GFF3697 0.60 +0.2 197,369 - GFF3697 0.60 +3.8 197,369 - GFF3697 0.60 +0.4 197,584 + GFF3697 0.73 -1.4 197,711 - GFF3697 0.80 -0.5 197,711 - GFF3697 0.80 -0.1 197,711 - GFF3697 0.80 -0.2 197,780 - GFF3697 0.85 -1.2 198,118 + +0.7 198,119 - -1.0 198,122 + -1.6 198,123 - -0.8 198,315 + GFF3698 0.10 +0.8 198,315 + GFF3698 0.10 +0.8 198,316 - GFF3698 0.10 +0.8 198,316 - GFF3698 0.10 +0.1 198,316 - GFF3698 0.10 -2.2 198,316 - GFF3698 0.10 +0.7 198,997 - GFF3698 0.35 +0.0 198,997 - GFF3698 0.35 +0.1 199,431 + GFF3698 0.51 -0.2 199,431 + GFF3698 0.51 -0.2 199,432 - GFF3698 0.51 +0.8 199,432 - GFF3698 0.51 +0.1 199,905 + GFF3698 0.68 +0.8 199,905 + GFF3698 0.68 -0.0 199,906 - GFF3698 0.68 +0.3 199,942 + GFF3698 0.69 -1.2 199,942 + GFF3698 0.69 -0.4 199,942 + GFF3698 0.69 -0.1 199,942 + GFF3698 0.69 +0.5 200,136 + GFF3698 0.76 +0.0 200,412 + GFF3698 0.86 +0.8 200,413 - GFF3698 0.86 +0.6 200,413 - GFF3698 0.86 -0.9 201,483 + GFF3700 0.24 -0.1
Or see this region's nucleotide sequence