Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3696 and GFF3697 overlap by 4 nucleotides GFF3697 and GFF3698 overlap by 4 nucleotides
GFF3696 - mRNA 3-end processing factor, at 195,299 to 196,381
GFF3696
GFF3697 - ATP-dependent DNA ligase (EC 6.5.1.1) LigC, at 196,378 to 198,033
GFF3697
GFF3698 - ATP-dependent, 3'-5' DNA helicase with strand annealing activity, at 198,030 to 200,786
GFF3698
Position (kb) 196
197
198
199 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 195.452 kb on + strand, within GFF3696 at 195.453 kb on - strand, within GFF3696 at 195.453 kb on - strand, within GFF3696 at 195.527 kb on + strand, within GFF3696 at 195.528 kb on - strand, within GFF3696 at 195.528 kb on - strand, within GFF3696 at 195.647 kb on + strand, within GFF3696 at 195.648 kb on - strand, within GFF3696 at 195.648 kb on - strand, within GFF3696 at 195.830 kb on + strand, within GFF3696 at 195.831 kb on - strand, within GFF3696 at 195.831 kb on - strand, within GFF3696 at 195.831 kb on - strand, within GFF3696 at 195.927 kb on - strand, within GFF3696 at 196.064 kb on + strand, within GFF3696 at 196.290 kb on - strand at 196.399 kb on + strand at 196.457 kb on - strand at 196.457 kb on - strand at 196.457 kb on - strand at 196.498 kb on + strand at 196.499 kb on - strand at 196.603 kb on + strand, within GFF3697 at 196.603 kb on + strand, within GFF3697 at 196.603 kb on + strand, within GFF3697 at 196.801 kb on + strand, within GFF3697 at 196.801 kb on + strand, within GFF3697 at 196.802 kb on - strand, within GFF3697 at 196.802 kb on - strand, within GFF3697 at 196.918 kb on + strand, within GFF3697 at 196.918 kb on + strand, within GFF3697 at 197.368 kb on + strand, within GFF3697 at 197.369 kb on - strand, within GFF3697 at 197.369 kb on - strand, within GFF3697 at 197.369 kb on - strand, within GFF3697 at 197.584 kb on + strand, within GFF3697 at 197.711 kb on - strand, within GFF3697 at 197.711 kb on - strand, within GFF3697 at 197.711 kb on - strand, within GFF3697 at 197.780 kb on - strand, within GFF3697 at 198.118 kb on + strand at 198.119 kb on - strand at 198.122 kb on + strand at 198.123 kb on - strand at 198.315 kb on + strand, within GFF3698 at 198.315 kb on + strand, within GFF3698 at 198.316 kb on - strand, within GFF3698 at 198.316 kb on - strand, within GFF3698 at 198.316 kb on - strand, within GFF3698 at 198.316 kb on - strand, within GFF3698 at 198.997 kb on - strand, within GFF3698 at 198.997 kb on - strand, within GFF3698
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 195,452 + GFF3696 0.14 +0.6 195,453 - GFF3696 0.14 -0.4 195,453 - GFF3696 0.14 +0.3 195,527 + GFF3696 0.21 +0.5 195,528 - GFF3696 0.21 +0.6 195,528 - GFF3696 0.21 +1.8 195,647 + GFF3696 0.32 +2.0 195,648 - GFF3696 0.32 +0.6 195,648 - GFF3696 0.32 -0.1 195,830 + GFF3696 0.49 -0.6 195,831 - GFF3696 0.49 -0.7 195,831 - GFF3696 0.49 -0.6 195,831 - GFF3696 0.49 +0.0 195,927 - GFF3696 0.58 +0.0 196,064 + GFF3696 0.71 +0.5 196,290 - -1.1 196,399 + -0.8 196,457 - -0.6 196,457 - -0.3 196,457 - +0.2 196,498 + +0.1 196,499 - +0.5 196,603 + GFF3697 0.14 -0.4 196,603 + GFF3697 0.14 -1.8 196,603 + GFF3697 0.14 -1.4 196,801 + GFF3697 0.26 +0.3 196,801 + GFF3697 0.26 -1.4 196,802 - GFF3697 0.26 -0.4 196,802 - GFF3697 0.26 +0.4 196,918 + GFF3697 0.33 -0.2 196,918 + GFF3697 0.33 +0.1 197,368 + GFF3697 0.60 -1.5 197,369 - GFF3697 0.60 +0.2 197,369 - GFF3697 0.60 +3.8 197,369 - GFF3697 0.60 +0.4 197,584 + GFF3697 0.73 -1.4 197,711 - GFF3697 0.80 -0.5 197,711 - GFF3697 0.80 -0.1 197,711 - GFF3697 0.80 -0.2 197,780 - GFF3697 0.85 -1.2 198,118 + +0.7 198,119 - -1.0 198,122 + -1.6 198,123 - -0.8 198,315 + GFF3698 0.10 +0.8 198,315 + GFF3698 0.10 +0.8 198,316 - GFF3698 0.10 +0.8 198,316 - GFF3698 0.10 +0.1 198,316 - GFF3698 0.10 -2.2 198,316 - GFF3698 0.10 +0.7 198,997 - GFF3698 0.35 +0.0 198,997 - GFF3698 0.35 +0.1
Or see this region's nucleotide sequence