Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3689 and GFF3690 overlap by 4 nucleotides GFF3690 and GFF3691 overlap by 41 nucleotides
GFF3689 - FIG01198523: hypothetical protein, at 187,944 to 189,146
GFF3689
GFF3690 - ABC transporter, ATP-binding protein (cluster 2, ribose/xylose/arabinose/galactose) / ABC transporter, ATP-binding protein (cluster 2, ribose/xylose/arabinose/galactose), at 189,143 to 190,690
GFF3690
GFF3691 - ABC transporter, permease protein (cluster 2, ribose/xylose/arabinose/galactose), at 190,650 to 191,684
GFF3691
Position (kb)
189
190
191 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 188.311 kb on + strand, within GFF3689 at 188.312 kb on - strand, within GFF3689 at 188.312 kb on - strand, within GFF3689 at 188.312 kb on - strand, within GFF3689 at 188.464 kb on - strand, within GFF3689 at 188.464 kb on - strand, within GFF3689 at 188.479 kb on - strand, within GFF3689 at 188.479 kb on - strand, within GFF3689 at 188.622 kb on + strand, within GFF3689 at 188.622 kb on + strand, within GFF3689 at 188.623 kb on - strand, within GFF3689 at 189.012 kb on + strand, within GFF3689 at 189.012 kb on + strand, within GFF3689 at 189.012 kb on + strand, within GFF3689 at 189.013 kb on - strand, within GFF3689 at 189.013 kb on - strand, within GFF3689 at 189.057 kb on + strand at 189.057 kb on + strand at 189.057 kb on + strand at 189.057 kb on + strand at 189.057 kb on + strand at 189.057 kb on + strand at 189.058 kb on - strand at 189.058 kb on - strand at 189.269 kb on + strand at 189.269 kb on + strand at 189.269 kb on + strand at 189.269 kb on + strand at 189.755 kb on + strand, within GFF3690 at 189.755 kb on + strand, within GFF3690 at 189.755 kb on + strand, within GFF3690 at 189.755 kb on + strand, within GFF3690 at 189.755 kb on + strand, within GFF3690 at 189.755 kb on + strand, within GFF3690 at 189.755 kb on + strand, within GFF3690 at 189.756 kb on - strand, within GFF3690 at 189.756 kb on - strand, within GFF3690 at 189.756 kb on - strand, within GFF3690 at 189.756 kb on - strand, within GFF3690 at 189.756 kb on - strand, within GFF3690 at 189.756 kb on - strand, within GFF3690 at 190.174 kb on + strand, within GFF3690 at 190.174 kb on + strand, within GFF3690 at 190.175 kb on - strand, within GFF3690 at 190.175 kb on - strand, within GFF3690 at 190.175 kb on - strand, within GFF3690 at 190.175 kb on - strand, within GFF3690 at 190.175 kb on - strand, within GFF3690 at 190.178 kb on + strand, within GFF3690 at 190.179 kb on - strand, within GFF3690 at 190.179 kb on - strand, within GFF3690 at 190.179 kb on - strand, within GFF3690 at 190.262 kb on + strand, within GFF3690 at 190.263 kb on - strand, within GFF3690 at 190.326 kb on - strand, within GFF3690 at 190.326 kb on - strand, within GFF3690 at 190.484 kb on + strand, within GFF3690 at 190.484 kb on + strand, within GFF3690 at 190.484 kb on + strand, within GFF3690 at 190.484 kb on + strand, within GFF3690 at 190.484 kb on + strand, within GFF3690 at 190.484 kb on + strand, within GFF3690 at 190.485 kb on - strand, within GFF3690 at 191.146 kb on - strand, within GFF3691 at 191.146 kb on - strand, within GFF3691 at 191.146 kb on - strand, within GFF3691 at 191.196 kb on + strand, within GFF3691 at 191.196 kb on + strand, within GFF3691 at 191.196 kb on + strand, within GFF3691 at 191.197 kb on - strand, within GFF3691 at 191.197 kb on - strand, within GFF3691 at 191.197 kb on - strand, within GFF3691 at 191.197 kb on - strand, within GFF3691 at 191.208 kb on + strand, within GFF3691 at 191.208 kb on + strand, within GFF3691 at 191.209 kb on - strand, within GFF3691 at 191.209 kb on - strand, within GFF3691 at 191.211 kb on + strand, within GFF3691 at 191.211 kb on + strand, within GFF3691 at 191.211 kb on + strand, within GFF3691 at 191.211 kb on + strand, within GFF3691 at 191.212 kb on - strand, within GFF3691 at 191.212 kb on - strand, within GFF3691 at 191.281 kb on + strand, within GFF3691 at 191.281 kb on + strand, within GFF3691 at 191.282 kb on - strand, within GFF3691 at 191.282 kb on - strand, within GFF3691 at 191.299 kb on - strand, within GFF3691 at 191.307 kb on + strand, within GFF3691 at 191.308 kb on - strand, within GFF3691 at 191.347 kb on - strand, within GFF3691 at 191.385 kb on + strand, within GFF3691 at 191.385 kb on + strand, within GFF3691 at 191.385 kb on + strand, within GFF3691 at 191.385 kb on + strand, within GFF3691 at 191.385 kb on + strand, within GFF3691 at 191.385 kb on + strand, within GFF3691 at 191.386 kb on - strand, within GFF3691 at 191.386 kb on - strand, within GFF3691 at 191.427 kb on + strand, within GFF3691 at 191.427 kb on + strand, within GFF3691 at 191.427 kb on + strand, within GFF3691 at 191.427 kb on + strand, within GFF3691 at 191.428 kb on - strand, within GFF3691 at 191.428 kb on - strand, within GFF3691 at 191.428 kb on - strand, within GFF3691 at 191.616 kb on + strand at 191.616 kb on + strand at 191.616 kb on + strand at 191.617 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 188,311 + GFF3689 0.31 +0.1 188,312 - GFF3689 0.31 +1.7 188,312 - GFF3689 0.31 +0.0 188,312 - GFF3689 0.31 -0.5 188,464 - GFF3689 0.43 -0.4 188,464 - GFF3689 0.43 -0.3 188,479 - GFF3689 0.44 +0.2 188,479 - GFF3689 0.44 -0.8 188,622 + GFF3689 0.56 -0.2 188,622 + GFF3689 0.56 -0.4 188,623 - GFF3689 0.56 -1.0 189,012 + GFF3689 0.89 +0.2 189,012 + GFF3689 0.89 -0.2 189,012 + GFF3689 0.89 -0.2 189,013 - GFF3689 0.89 +0.8 189,013 - GFF3689 0.89 +0.0 189,057 + -0.2 189,057 + +0.4 189,057 + -0.1 189,057 + -0.6 189,057 + +0.8 189,057 + -0.7 189,058 - -0.4 189,058 - -1.0 189,269 + -0.2 189,269 + +0.1 189,269 + -0.4 189,269 + +0.2 189,755 + GFF3690 0.40 -0.8 189,755 + GFF3690 0.40 -0.6 189,755 + GFF3690 0.40 -0.1 189,755 + GFF3690 0.40 +0.2 189,755 + GFF3690 0.40 -0.1 189,755 + GFF3690 0.40 -1.0 189,755 + GFF3690 0.40 -0.8 189,756 - GFF3690 0.40 +0.2 189,756 - GFF3690 0.40 -0.5 189,756 - GFF3690 0.40 -0.7 189,756 - GFF3690 0.40 +0.6 189,756 - GFF3690 0.40 +0.0 189,756 - GFF3690 0.40 +0.8 190,174 + GFF3690 0.67 -0.3 190,174 + GFF3690 0.67 -0.9 190,175 - GFF3690 0.67 -0.3 190,175 - GFF3690 0.67 +1.0 190,175 - GFF3690 0.67 -0.5 190,175 - GFF3690 0.67 -0.7 190,175 - GFF3690 0.67 -0.3 190,178 + GFF3690 0.67 -0.2 190,179 - GFF3690 0.67 +0.1 190,179 - GFF3690 0.67 +2.4 190,179 - GFF3690 0.67 +0.1 190,262 + GFF3690 0.72 -1.0 190,263 - GFF3690 0.72 -1.1 190,326 - GFF3690 0.76 -0.8 190,326 - GFF3690 0.76 -0.5 190,484 + GFF3690 0.87 -0.2 190,484 + GFF3690 0.87 -1.0 190,484 + GFF3690 0.87 -0.9 190,484 + GFF3690 0.87 -0.0 190,484 + GFF3690 0.87 +0.2 190,484 + GFF3690 0.87 +0.0 190,485 - GFF3690 0.87 -0.2 191,146 - GFF3691 0.48 +0.1 191,146 - GFF3691 0.48 -1.8 191,146 - GFF3691 0.48 -0.3 191,196 + GFF3691 0.53 -0.8 191,196 + GFF3691 0.53 -0.5 191,196 + GFF3691 0.53 -0.7 191,197 - GFF3691 0.53 -0.1 191,197 - GFF3691 0.53 -2.3 191,197 - GFF3691 0.53 -0.2 191,197 - GFF3691 0.53 +1.5 191,208 + GFF3691 0.54 +0.2 191,208 + GFF3691 0.54 -0.2 191,209 - GFF3691 0.54 +0.8 191,209 - GFF3691 0.54 +0.0 191,211 + GFF3691 0.54 -0.4 191,211 + GFF3691 0.54 -0.8 191,211 + GFF3691 0.54 +0.3 191,211 + GFF3691 0.54 -0.2 191,212 - GFF3691 0.54 -1.2 191,212 - GFF3691 0.54 +0.5 191,281 + GFF3691 0.61 -0.1 191,281 + GFF3691 0.61 +1.0 191,282 - GFF3691 0.61 -2.2 191,282 - GFF3691 0.61 +0.2 191,299 - GFF3691 0.63 -1.1 191,307 + GFF3691 0.63 -0.2 191,308 - GFF3691 0.64 +0.2 191,347 - GFF3691 0.67 +0.0 191,385 + GFF3691 0.71 +0.1 191,385 + GFF3691 0.71 +0.0 191,385 + GFF3691 0.71 +0.6 191,385 + GFF3691 0.71 +1.3 191,385 + GFF3691 0.71 +0.2 191,385 + GFF3691 0.71 -0.4 191,386 - GFF3691 0.71 +0.3 191,386 - GFF3691 0.71 +1.2 191,427 + GFF3691 0.75 +0.1 191,427 + GFF3691 0.75 +0.8 191,427 + GFF3691 0.75 +0.3 191,427 + GFF3691 0.75 -1.0 191,428 - GFF3691 0.75 -0.0 191,428 - GFF3691 0.75 +0.6 191,428 - GFF3691 0.75 -1.5 191,616 + -0.4 191,616 + +0.8 191,616 + -0.8 191,617 - -0.9
Or see this region's nucleotide sequence