Strain Fitness in Variovorax sp. SCN45 around GFF3543

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3541 and GFF3542 overlap by 4 nucleotidesGFF3542 and GFF3543 are separated by 5 nucleotidesGFF3543 and GFF3544 overlap by 8 nucleotidesGFF3544 and GFF3545 are separated by 43 nucleotides GFF3541 - hypothetical protein, at 46,515 to 47,342 GFF3541 GFF3542 - hypothetical protein, at 47,339 to 47,851 GFF3542 GFF3543 - no description, at 47,857 to 48,453 GFF3543 GFF3544 - no description, at 48,446 to 49,057 GFF3544 GFF3545 - hypothetical protein, at 49,101 to 51,404 GFF3545 Position (kb) 47 48 49Strain fitness (log2 ratio) -2 -1 0 1 2 3at 46.927 kb on + strand, within GFF3541at 47.102 kb on - strand, within GFF3541at 47.125 kb on + strand, within GFF3541at 47.125 kb on + strand, within GFF3541at 47.125 kb on + strand, within GFF3541at 47.125 kb on + strand, within GFF3541at 47.126 kb on - strand, within GFF3541at 47.126 kb on - strand, within GFF3541at 47.354 kb on + strandat 47.354 kb on + strandat 47.354 kb on + strandat 47.355 kb on - strandat 47.355 kb on - strandat 47.365 kb on - strandat 47.365 kb on - strandat 47.365 kb on - strandat 47.592 kb on + strand, within GFF3542at 47.592 kb on + strand, within GFF3542at 47.592 kb on + strand, within GFF3542at 47.592 kb on + strand, within GFF3542at 47.592 kb on + strand, within GFF3542at 47.592 kb on + strand, within GFF3542at 47.592 kb on + strand, within GFF3542at 47.592 kb on + strand, within GFF3542at 47.592 kb on + strand, within GFF3542at 47.592 kb on + strand, within GFF3542at 47.592 kb on + strand, within GFF3542at 47.592 kb on + strand, within GFF3542at 47.592 kb on + strand, within GFF3542at 47.593 kb on - strand, within GFF3542at 47.593 kb on - strand, within GFF3542at 47.593 kb on - strand, within GFF3542at 47.593 kb on - strand, within GFF3542at 47.593 kb on - strand, within GFF3542at 47.593 kb on - strand, within GFF3542at 47.595 kb on + strand, within GFF3542at 47.595 kb on + strand, within GFF3542at 47.595 kb on + strand, within GFF3542at 47.595 kb on + strand, within GFF3542at 47.595 kb on + strand, within GFF3542at 47.595 kb on + strand, within GFF3542at 47.595 kb on + strand, within GFF3542at 47.595 kb on + strand, within GFF3542at 47.595 kb on + strand, within GFF3542at 47.596 kb on - strand, within GFF3542at 47.596 kb on - strand, within GFF3542at 47.596 kb on - strand, within GFF3542at 47.596 kb on - strand, within GFF3542at 47.596 kb on - strand, within GFF3542at 47.596 kb on - strand, within GFF3542at 47.596 kb on - strand, within GFF3542at 47.596 kb on - strand, within GFF3542at 47.596 kb on - strand, within GFF3542at 47.619 kb on + strand, within GFF3542at 47.619 kb on + strand, within GFF3542at 47.619 kb on + strand, within GFF3542at 47.619 kb on + strand, within GFF3542at 47.619 kb on + strand, within GFF3542at 47.620 kb on - strand, within GFF3542at 47.620 kb on - strand, within GFF3542at 47.620 kb on - strand, within GFF3542at 47.894 kb on + strandat 47.894 kb on + strandat 47.894 kb on + strandat 47.894 kb on + strandat 47.895 kb on - strandat 47.895 kb on - strandat 47.909 kb on + strandat 47.909 kb on + strandat 47.910 kb on - strandat 47.927 kb on + strand, within GFF3543at 47.927 kb on + strand, within GFF3543at 47.928 kb on - strand, within GFF3543at 47.928 kb on - strand, within GFF3543at 47.928 kb on - strand, within GFF3543at 48.049 kb on + strand, within GFF3543at 48.049 kb on + strand, within GFF3543at 48.049 kb on + strand, within GFF3543at 48.049 kb on + strand, within GFF3543at 48.049 kb on + strand, within GFF3543at 48.049 kb on + strand, within GFF3543at 48.049 kb on + strand, within GFF3543at 48.049 kb on + strand, within GFF3543at 48.050 kb on - strand, within GFF3543at 48.050 kb on - strand, within GFF3543at 48.050 kb on - strand, within GFF3543at 48.050 kb on - strand, within GFF3543at 48.050 kb on - strand, within GFF3543at 48.050 kb on - strand, within GFF3543at 48.050 kb on - strand, within GFF3543at 48.050 kb on - strand, within GFF3543at 48.050 kb on - strand, within GFF3543at 48.050 kb on - strand, within GFF3543at 48.331 kb on + strand, within GFF3543at 48.331 kb on + strand, within GFF3543at 48.331 kb on + strand, within GFF3543at 48.331 kb on + strand, within GFF3543at 48.332 kb on - strand, within GFF3543at 48.332 kb on - strand, within GFF3543at 48.332 kb on - strand, within GFF3543at 48.350 kb on + strand, within GFF3543at 48.374 kb on + strand, within GFF3543at 48.374 kb on + strand, within GFF3543at 48.374 kb on + strand, within GFF3543at 48.374 kb on + strand, within GFF3543at 48.375 kb on - strand, within GFF3543at 48.375 kb on - strand, within GFF3543at 48.375 kb on - strand, within GFF3543at 48.375 kb on - strand, within GFF3543at 48.465 kb on + strandat 48.465 kb on + strandat 48.465 kb on + strandat 48.465 kb on + strandat 48.465 kb on + strandat 48.465 kb on + strandat 48.465 kb on + strandat 48.466 kb on - strandat 48.466 kb on - strandat 48.466 kb on - strandat 48.466 kb on - strandat 48.483 kb on + strandat 48.483 kb on + strandat 48.483 kb on + strandat 48.483 kb on + strandat 48.483 kb on + strandat 48.483 kb on + strandat 48.484 kb on - strandat 48.484 kb on - strandat 48.484 kb on - strandat 48.687 kb on + strand, within GFF3544at 48.687 kb on + strand, within GFF3544at 48.687 kb on + strand, within GFF3544at 48.687 kb on + strand, within GFF3544at 48.687 kb on + strand, within GFF3544at 48.687 kb on + strand, within GFF3544at 48.688 kb on - strand, within GFF3544at 48.688 kb on - strand, within GFF3544at 48.966 kb on + strand, within GFF3544at 48.966 kb on + strand, within GFF3544at 48.972 kb on + strand, within GFF3544at 48.972 kb on + strand, within GFF3544at 48.973 kb on - strand, within GFF3544at 49.074 kb on + strandat 49.369 kb on + strand, within GFF3545at 49.369 kb on + strand, within GFF3545at 49.369 kb on + strand, within GFF3545at 49.370 kb on - strand, within GFF3545at 49.432 kb on + strand, within GFF3545at 49.432 kb on + strand, within GFF3545at 49.433 kb on - strand, within GFF3545

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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46,927 + GFF3541 0.50 -0.1
47,102 - GFF3541 0.71 -0.1
47,125 + GFF3541 0.74 +0.0
47,125 + GFF3541 0.74 +0.8
47,125 + GFF3541 0.74 -0.8
47,125 + GFF3541 0.74 -0.1
47,126 - GFF3541 0.74 -0.6
47,126 - GFF3541 0.74 -0.0
47,354 + +0.4
47,354 + -1.4
47,354 + -0.4
47,355 - -0.4
47,355 - -0.6
47,365 - -2.0
47,365 - +0.5
47,365 - -1.4
47,592 + GFF3542 0.49 -0.6
47,592 + GFF3542 0.49 +0.4
47,592 + GFF3542 0.49 -1.0
47,592 + GFF3542 0.49 +0.8
47,592 + GFF3542 0.49 +0.8
47,592 + GFF3542 0.49 -0.1
47,592 + GFF3542 0.49 +0.1
47,592 + GFF3542 0.49 -0.6
47,592 + GFF3542 0.49 -1.4
47,592 + GFF3542 0.49 +2.8
47,592 + GFF3542 0.49 -0.7
47,592 + GFF3542 0.49 -0.8
47,592 + GFF3542 0.49 -0.4
47,593 - GFF3542 0.50 -1.2
47,593 - GFF3542 0.50 +0.2
47,593 - GFF3542 0.50 +0.2
47,593 - GFF3542 0.50 +0.2
47,593 - GFF3542 0.50 -0.5
47,593 - GFF3542 0.50 -0.1
47,595 + GFF3542 0.50 -0.0
47,595 + GFF3542 0.50 -0.1
47,595 + GFF3542 0.50 -0.0
47,595 + GFF3542 0.50 +0.2
47,595 + GFF3542 0.50 +0.2
47,595 + GFF3542 0.50 -0.3
47,595 + GFF3542 0.50 -0.5
47,595 + GFF3542 0.50 +1.1
47,595 + GFF3542 0.50 +0.1
47,596 - GFF3542 0.50 -0.1
47,596 - GFF3542 0.50 -0.2
47,596 - GFF3542 0.50 -0.4
47,596 - GFF3542 0.50 +0.4
47,596 - GFF3542 0.50 -0.4
47,596 - GFF3542 0.50 -0.5
47,596 - GFF3542 0.50 -1.8
47,596 - GFF3542 0.50 -0.4
47,596 - GFF3542 0.50 -1.2
47,619 + GFF3542 0.55 +0.1
47,619 + GFF3542 0.55 -0.7
47,619 + GFF3542 0.55 -0.2
47,619 + GFF3542 0.55 -0.5
47,619 + GFF3542 0.55 -0.8
47,620 - GFF3542 0.55 -0.1
47,620 - GFF3542 0.55 +0.2
47,620 - GFF3542 0.55 -0.5
47,894 + -0.8
47,894 + -0.1
47,894 + -0.4
47,894 + -0.5
47,895 - +0.4
47,895 - +0.9
47,909 + -0.1
47,909 + -0.0
47,910 - +0.1
47,927 + GFF3543 0.12 -1.0
47,927 + GFF3543 0.12 -1.0
47,928 - GFF3543 0.12 -0.0
47,928 - GFF3543 0.12 -0.5
47,928 - GFF3543 0.12 +0.0
48,049 + GFF3543 0.32 +1.1
48,049 + GFF3543 0.32 -0.6
48,049 + GFF3543 0.32 -0.2
48,049 + GFF3543 0.32 +0.5
48,049 + GFF3543 0.32 -0.5
48,049 + GFF3543 0.32 -0.2
48,049 + GFF3543 0.32 +1.8
48,049 + GFF3543 0.32 -1.0
48,050 - GFF3543 0.32 -0.0
48,050 - GFF3543 0.32 +0.8
48,050 - GFF3543 0.32 +1.0
48,050 - GFF3543 0.32 +0.5
48,050 - GFF3543 0.32 -0.4
48,050 - GFF3543 0.32 -0.8
48,050 - GFF3543 0.32 +0.4
48,050 - GFF3543 0.32 -0.8
48,050 - GFF3543 0.32 +0.7
48,050 - GFF3543 0.32 -0.2
48,331 + GFF3543 0.79 -0.2
48,331 + GFF3543 0.79 -0.3
48,331 + GFF3543 0.79 -0.6
48,331 + GFF3543 0.79 +0.3
48,332 - GFF3543 0.80 +0.2
48,332 - GFF3543 0.80 +0.4
48,332 - GFF3543 0.80 +0.2
48,350 + GFF3543 0.83 +0.3
48,374 + GFF3543 0.87 +0.5
48,374 + GFF3543 0.87 -0.3
48,374 + GFF3543 0.87 +0.1
48,374 + GFF3543 0.87 +1.2
48,375 - GFF3543 0.87 -0.1
48,375 - GFF3543 0.87 -0.7
48,375 - GFF3543 0.87 +1.5
48,375 - GFF3543 0.87 -0.2
48,465 + +1.8
48,465 + -0.5
48,465 + -1.5
48,465 + -0.5
48,465 + -0.2
48,465 + +0.1
48,465 + +0.5
48,466 - -2.1
48,466 - +0.5
48,466 - -0.8
48,466 - +0.1
48,483 + -0.2
48,483 + +0.1
48,483 + +0.4
48,483 + +0.0
48,483 + -0.2
48,483 + -0.3
48,484 - -0.3
48,484 - -0.6
48,484 - -0.5
48,687 + GFF3544 0.39 +0.8
48,687 + GFF3544 0.39 -0.3
48,687 + GFF3544 0.39 -0.3
48,687 + GFF3544 0.39 -0.5
48,687 + GFF3544 0.39 -0.8
48,687 + GFF3544 0.39 -0.0
48,688 - GFF3544 0.40 -1.5
48,688 - GFF3544 0.40 +0.4
48,966 + GFF3544 0.85 +0.0
48,966 + GFF3544 0.85 +0.3
48,972 + GFF3544 0.86 -1.2
48,972 + GFF3544 0.86 -0.6
48,973 - GFF3544 0.86 -1.0
49,074 + -0.5
49,369 + GFF3545 0.12 +0.3
49,369 + GFF3545 0.12 +0.4
49,369 + GFF3545 0.12 +0.0
49,370 - GFF3545 0.12 +0.4
49,432 + GFF3545 0.14 -0.4
49,432 + GFF3545 0.14 +0.2
49,433 - GFF3545 0.14 +0.2

Or see this region's nucleotide sequence