Strain Fitness in Variovorax sp. SCN45 around GFF3424

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3423 and GFF3424 are separated by 213 nucleotidesGFF3424 and GFF3425 are separated by 104 nucleotidesGFF3425 and GFF3426 overlap by 4 nucleotides GFF3423 - Adenylate cyclase (EC 4.6.1.1), at 246,851 to 248,785 GFF3423 GFF3424 - FIG00443816: hypothetical protein, at 248,999 to 249,709 GFF3424 GFF3425 - no description, at 249,814 to 250,161 GFF3425 GFF3426 - Predicted kinase, at 250,158 to 250,973 GFF3426 Position (kb) 248 249 250Strain fitness (log2 ratio) -1 0 1 2 3at 248.110 kb on - strand, within GFF3423at 248.110 kb on - strand, within GFF3423at 248.145 kb on + strand, within GFF3423at 248.145 kb on + strand, within GFF3423at 248.145 kb on + strand, within GFF3423at 248.145 kb on + strand, within GFF3423at 248.146 kb on - strand, within GFF3423at 248.146 kb on - strand, within GFF3423at 248.190 kb on + strand, within GFF3423at 248.190 kb on + strand, within GFF3423at 248.190 kb on + strand, within GFF3423at 248.190 kb on + strand, within GFF3423at 248.191 kb on - strand, within GFF3423at 248.191 kb on - strand, within GFF3423at 248.214 kb on + strand, within GFF3423at 248.214 kb on + strand, within GFF3423at 248.214 kb on + strand, within GFF3423at 248.214 kb on + strand, within GFF3423at 248.215 kb on - strand, within GFF3423at 248.215 kb on - strand, within GFF3423at 248.215 kb on - strand, within GFF3423at 248.215 kb on - strand, within GFF3423at 248.250 kb on + strand, within GFF3423at 248.250 kb on + strand, within GFF3423at 248.250 kb on + strand, within GFF3423at 248.292 kb on + strand, within GFF3423at 248.292 kb on + strand, within GFF3423at 248.292 kb on + strand, within GFF3423at 248.293 kb on - strand, within GFF3423at 248.293 kb on - strand, within GFF3423at 248.293 kb on - strand, within GFF3423at 248.356 kb on - strand, within GFF3423at 248.397 kb on + strand, within GFF3423at 248.397 kb on + strand, within GFF3423at 248.397 kb on + strand, within GFF3423at 248.398 kb on - strand, within GFF3423at 248.398 kb on - strand, within GFF3423at 248.398 kb on - strand, within GFF3423at 248.398 kb on - strand, within GFF3423at 248.398 kb on - strand, within GFF3423at 248.582 kb on + strand, within GFF3423at 248.619 kb on + strandat 248.619 kb on + strandat 248.620 kb on - strandat 248.620 kb on - strandat 248.684 kb on + strandat 248.684 kb on + strandat 248.684 kb on + strandat 248.685 kb on - strandat 248.685 kb on - strandat 248.685 kb on - strandat 248.785 kb on + strandat 248.785 kb on + strandat 248.785 kb on + strandat 248.785 kb on + strandat 248.786 kb on - strandat 248.786 kb on - strandat 248.786 kb on - strandat 248.786 kb on - strandat 248.786 kb on - strandat 248.786 kb on - strandat 249.031 kb on + strandat 249.032 kb on - strandat 249.032 kb on - strandat 249.084 kb on + strand, within GFF3424at 249.085 kb on - strand, within GFF3424at 249.303 kb on + strand, within GFF3424at 249.303 kb on + strand, within GFF3424at 249.303 kb on + strand, within GFF3424at 249.304 kb on - strand, within GFF3424at 249.304 kb on - strand, within GFF3424at 249.304 kb on - strand, within GFF3424at 249.319 kb on - strand, within GFF3424at 249.375 kb on + strand, within GFF3424at 249.376 kb on - strand, within GFF3424at 249.456 kb on + strand, within GFF3424at 249.456 kb on + strand, within GFF3424at 249.456 kb on + strand, within GFF3424at 249.457 kb on - strand, within GFF3424at 249.507 kb on + strand, within GFF3424at 249.507 kb on + strand, within GFF3424at 249.507 kb on + strand, within GFF3424at 249.508 kb on - strand, within GFF3424at 249.508 kb on - strand, within GFF3424at 249.513 kb on + strand, within GFF3424at 249.579 kb on + strand, within GFF3424at 249.579 kb on + strand, within GFF3424at 249.579 kb on + strand, within GFF3424at 249.579 kb on + strand, within GFF3424at 249.579 kb on + strand, within GFF3424at 249.579 kb on + strand, within GFF3424at 249.579 kb on + strand, within GFF3424at 249.579 kb on + strand, within GFF3424at 249.580 kb on - strand, within GFF3424at 249.580 kb on - strand, within GFF3424at 249.580 kb on - strand, within GFF3424at 249.580 kb on - strand, within GFF3424at 249.580 kb on - strand, within GFF3424at 249.580 kb on - strand, within GFF3424at 250.057 kb on + strand, within GFF3425at 250.057 kb on + strand, within GFF3425at 250.058 kb on - strand, within GFF3425at 250.269 kb on + strand, within GFF3426at 250.269 kb on + strand, within GFF3426at 250.569 kb on + strand, within GFF3426at 250.569 kb on + strand, within GFF3426

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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248,110 - GFF3423 0.65 +0.5
248,110 - GFF3423 0.65 -0.5
248,145 + GFF3423 0.67 +0.7
248,145 + GFF3423 0.67 -0.4
248,145 + GFF3423 0.67 +2.9
248,145 + GFF3423 0.67 -0.1
248,146 - GFF3423 0.67 +0.1
248,146 - GFF3423 0.67 +0.4
248,190 + GFF3423 0.69 +0.3
248,190 + GFF3423 0.69 -1.1
248,190 + GFF3423 0.69 -1.6
248,190 + GFF3423 0.69 -0.8
248,191 - GFF3423 0.69 -0.1
248,191 - GFF3423 0.69 -0.6
248,214 + GFF3423 0.70 -0.1
248,214 + GFF3423 0.70 +0.5
248,214 + GFF3423 0.70 -0.8
248,214 + GFF3423 0.70 -0.8
248,215 - GFF3423 0.70 -1.1
248,215 - GFF3423 0.70 -0.1
248,215 - GFF3423 0.70 +0.1
248,215 - GFF3423 0.70 -0.5
248,250 + GFF3423 0.72 -0.5
248,250 + GFF3423 0.72 -0.0
248,250 + GFF3423 0.72 +1.3
248,292 + GFF3423 0.74 +1.1
248,292 + GFF3423 0.74 +0.1
248,292 + GFF3423 0.74 +0.1
248,293 - GFF3423 0.75 -1.0
248,293 - GFF3423 0.75 +1.9
248,293 - GFF3423 0.75 -0.1
248,356 - GFF3423 0.78 -0.5
248,397 + GFF3423 0.80 +0.3
248,397 + GFF3423 0.80 +0.5
248,397 + GFF3423 0.80 -0.7
248,398 - GFF3423 0.80 +0.1
248,398 - GFF3423 0.80 -0.1
248,398 - GFF3423 0.80 -1.3
248,398 - GFF3423 0.80 -0.7
248,398 - GFF3423 0.80 -1.5
248,582 + GFF3423 0.89 -1.1
248,619 + -0.5
248,619 + -0.4
248,620 - +0.2
248,620 - -0.4
248,684 + +0.4
248,684 + -0.3
248,684 + +0.1
248,685 - +0.2
248,685 - -0.1
248,685 - -1.1
248,785 + -0.7
248,785 + -0.0
248,785 + -1.1
248,785 + +0.5
248,786 - +0.4
248,786 - +0.1
248,786 - -1.5
248,786 - -0.5
248,786 - -0.5
248,786 - -0.7
249,031 + +0.1
249,032 - +0.1
249,032 - -0.2
249,084 + GFF3424 0.12 +0.2
249,085 - GFF3424 0.12 +1.4
249,303 + GFF3424 0.43 -1.3
249,303 + GFF3424 0.43 +1.5
249,303 + GFF3424 0.43 -0.1
249,304 - GFF3424 0.43 -1.4
249,304 - GFF3424 0.43 -0.1
249,304 - GFF3424 0.43 +0.7
249,319 - GFF3424 0.45 -0.2
249,375 + GFF3424 0.53 +0.4
249,376 - GFF3424 0.53 +0.2
249,456 + GFF3424 0.64 -0.6
249,456 + GFF3424 0.64 +0.3
249,456 + GFF3424 0.64 +0.9
249,457 - GFF3424 0.64 -0.3
249,507 + GFF3424 0.71 -0.4
249,507 + GFF3424 0.71 -0.1
249,507 + GFF3424 0.71 +0.1
249,508 - GFF3424 0.72 +0.2
249,508 - GFF3424 0.72 -1.3
249,513 + GFF3424 0.72 +0.6
249,579 + GFF3424 0.82 +0.1
249,579 + GFF3424 0.82 -0.3
249,579 + GFF3424 0.82 -0.8
249,579 + GFF3424 0.82 +0.5
249,579 + GFF3424 0.82 -0.7
249,579 + GFF3424 0.82 +0.6
249,579 + GFF3424 0.82 -0.1
249,579 + GFF3424 0.82 +0.4
249,580 - GFF3424 0.82 +0.7
249,580 - GFF3424 0.82 -1.0
249,580 - GFF3424 0.82 +0.4
249,580 - GFF3424 0.82 -0.8
249,580 - GFF3424 0.82 -0.3
249,580 - GFF3424 0.82 +0.3
250,057 + GFF3425 0.70 -0.9
250,057 + GFF3425 0.70 -0.1
250,058 - GFF3425 0.70 -0.1
250,269 + GFF3426 0.14 -0.1
250,269 + GFF3426 0.14 +0.1
250,569 + GFF3426 0.50 +0.1
250,569 + GFF3426 0.50 -0.8

Or see this region's nucleotide sequence