Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3344 and GFF3345 are separated by 26 nucleotides GFF3345 and GFF3346 overlap by 4 nucleotides GFF3346 and GFF3347 are separated by 121 nucleotides GFF3347 and GFF3348 are separated by 4 nucleotides
GFF3344 - Glucosamine 6-phosphate synthetase, contains amidotransferase and phosphosugar isomerase domains, at 168,411 to 168,929
GFF3344
GFF3345 - ATP:Cob(I)alamin adenosyltransferase (EC 2.5.1.17) @ ATP:Cob(I)alamin adenosyltransferase (EC 2.5.1.17), glycolate utilization, at 168,956 to 169,555
GFF3345
GFF3346 - hypothetical protein, at 169,552 to 169,914
GFF3346
GFF3347 - Adenylate cyclase (EC 4.6.1.1), at 170,036 to 170,557
GFF3347
GFF3348 - Putative lipase, at 170,562 to 171,203
GFF3348
Position (kb) 169
170 Strain fitness (log2 ratio) -2
-1
0
1
2 at 168.606 kb on + strand, within GFF3344 at 168.607 kb on - strand, within GFF3344 at 168.607 kb on - strand, within GFF3344 at 168.607 kb on - strand, within GFF3344 at 168.693 kb on + strand, within GFF3344 at 168.693 kb on + strand, within GFF3344 at 168.693 kb on + strand, within GFF3344 at 168.694 kb on - strand, within GFF3344 at 168.813 kb on + strand, within GFF3344 at 168.813 kb on + strand, within GFF3344 at 168.846 kb on + strand, within GFF3344 at 168.846 kb on + strand, within GFF3344 at 168.846 kb on + strand, within GFF3344 at 168.847 kb on - strand, within GFF3344 at 168.855 kb on - strand, within GFF3344 at 168.855 kb on - strand, within GFF3344 at 168.928 kb on - strand at 168.933 kb on + strand at 168.933 kb on + strand at 168.933 kb on + strand at 168.933 kb on + strand at 168.933 kb on + strand at 168.933 kb on + strand at 168.933 kb on + strand at 168.933 kb on + strand at 168.933 kb on + strand at 168.934 kb on - strand at 168.934 kb on - strand at 168.934 kb on - strand at 168.934 kb on - strand at 168.934 kb on - strand at 168.937 kb on + strand at 168.937 kb on + strand at 168.937 kb on + strand at 168.937 kb on + strand at 168.937 kb on + strand at 168.938 kb on - strand at 168.938 kb on - strand at 168.938 kb on - strand at 168.938 kb on - strand at 168.938 kb on - strand at 168.938 kb on - strand at 168.938 kb on - strand at 168.938 kb on - strand at 168.938 kb on - strand at 169.406 kb on + strand, within GFF3345 at 169.481 kb on + strand, within GFF3345 at 169.913 kb on - strand at 169.924 kb on - strand at 169.924 kb on - strand at 170.125 kb on - strand, within GFF3347 at 170.223 kb on - strand, within GFF3347 at 170.261 kb on + strand, within GFF3347 at 170.262 kb on - strand, within GFF3347 at 170.262 kb on - strand, within GFF3347 at 170.528 kb on + strand at 170.529 kb on - strand at 170.618 kb on - strand at 170.680 kb on + strand, within GFF3348 at 170.680 kb on + strand, within GFF3348 at 170.681 kb on - strand, within GFF3348 at 170.681 kb on - strand, within GFF3348 at 170.728 kb on + strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.729 kb on - strand, within GFF3348 at 170.729 kb on - strand, within GFF3348
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 168,606 + GFF3344 0.38 -1.1 168,607 - GFF3344 0.38 -0.4 168,607 - GFF3344 0.38 -0.3 168,607 - GFF3344 0.38 +0.9 168,693 + GFF3344 0.54 -1.6 168,693 + GFF3344 0.54 +0.2 168,693 + GFF3344 0.54 -0.1 168,694 - GFF3344 0.55 -0.7 168,813 + GFF3344 0.77 +0.2 168,813 + GFF3344 0.77 +0.6 168,846 + GFF3344 0.84 -0.0 168,846 + GFF3344 0.84 -0.1 168,846 + GFF3344 0.84 -0.4 168,847 - GFF3344 0.84 +0.9 168,855 - GFF3344 0.86 +0.2 168,855 - GFF3344 0.86 +0.1 168,928 - -1.6 168,933 + +0.9 168,933 + +1.9 168,933 + -0.9 168,933 + -0.3 168,933 + -1.3 168,933 + -0.6 168,933 + +0.8 168,933 + -0.4 168,933 + -0.6 168,934 - -0.5 168,934 - -0.4 168,934 - +0.1 168,934 - +0.0 168,934 - -0.1 168,937 + -0.7 168,937 + -0.9 168,937 + +0.0 168,937 + -0.7 168,937 + +0.9 168,938 - -0.3 168,938 - +0.2 168,938 - -2.3 168,938 - -0.8 168,938 - -0.4 168,938 - -0.3 168,938 - +0.3 168,938 - -1.1 168,938 - -1.2 169,406 + GFF3345 0.75 -0.7 169,481 + GFF3345 0.88 -0.4 169,913 - +0.3 169,924 - +0.8 169,924 - -0.9 170,125 - GFF3347 0.17 -1.4 170,223 - GFF3347 0.36 +1.5 170,261 + GFF3347 0.43 +0.5 170,262 - GFF3347 0.43 -0.9 170,262 - GFF3347 0.43 -0.6 170,528 + +0.1 170,529 - +0.4 170,618 - -0.1 170,680 + GFF3348 0.18 -0.4 170,680 + GFF3348 0.18 -0.1 170,681 - GFF3348 0.19 -1.5 170,681 - GFF3348 0.19 -0.1 170,728 + GFF3348 0.26 -0.2 170,729 - GFF3348 0.26 +0.2 170,729 - GFF3348 0.26 -0.2 170,729 - GFF3348 0.26 +1.3 170,729 - GFF3348 0.26 +0.4 170,729 - GFF3348 0.26 -0.3
Or see this region's nucleotide sequence