Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF327 and GFF328 are separated by 44 nucleotides GFF328 and GFF329 overlap by 4 nucleotides
GFF327 - 5'-methylthioadenosine nucleosidase (EC 3.2.2.16) @ S-adenosylhomocysteine nucleosidase (EC 3.2.2.9), at 361,222 to 362,199
GFF327
GFF328 - hypothetical protein, at 362,244 to 362,939
GFF328
GFF329 - Nucleoside ABC transporter, ATP-binding protein, at 362,936 to 364,537
GFF329
Position (kb) 362
363 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 361.324 kb on + strand, within GFF327 at 361.324 kb on + strand, within GFF327 at 361.324 kb on + strand, within GFF327 at 361.324 kb on + strand, within GFF327 at 361.325 kb on - strand, within GFF327 at 361.666 kb on + strand, within GFF327 at 361.667 kb on - strand, within GFF327 at 361.667 kb on - strand, within GFF327 at 361.708 kb on + strand, within GFF327 at 361.708 kb on + strand, within GFF327 at 361.708 kb on + strand, within GFF327 at 361.709 kb on - strand, within GFF327 at 361.709 kb on - strand, within GFF327 at 361.709 kb on - strand, within GFF327 at 361.975 kb on + strand, within GFF327 at 361.984 kb on + strand, within GFF327 at 361.984 kb on + strand, within GFF327 at 361.984 kb on + strand, within GFF327 at 361.984 kb on + strand, within GFF327 at 361.984 kb on + strand, within GFF327 at 361.984 kb on + strand, within GFF327 at 361.985 kb on - strand, within GFF327 at 361.985 kb on - strand, within GFF327 at 361.985 kb on - strand, within GFF327 at 361.990 kb on + strand, within GFF327 at 361.990 kb on + strand, within GFF327 at 361.990 kb on + strand, within GFF327 at 361.990 kb on + strand, within GFF327 at 361.990 kb on + strand, within GFF327 at 361.990 kb on + strand, within GFF327 at 361.990 kb on + strand, within GFF327 at 361.990 kb on + strand, within GFF327 at 361.990 kb on + strand, within GFF327 at 361.990 kb on + strand, within GFF327 at 361.990 kb on + strand, within GFF327 at 361.990 kb on + strand, within GFF327 at 361.990 kb on + strand, within GFF327 at 361.990 kb on + strand, within GFF327 at 361.990 kb on + strand, within GFF327 at 361.991 kb on - strand, within GFF327 at 361.991 kb on - strand, within GFF327 at 361.991 kb on - strand, within GFF327 at 361.991 kb on - strand, within GFF327 at 361.991 kb on - strand, within GFF327 at 361.991 kb on - strand, within GFF327 at 361.991 kb on - strand, within GFF327 at 361.991 kb on - strand, within GFF327 at 361.991 kb on - strand, within GFF327 at 361.991 kb on - strand, within GFF327 at 361.991 kb on - strand, within GFF327 at 362.050 kb on + strand, within GFF327 at 362.051 kb on - strand, within GFF327 at 362.051 kb on - strand, within GFF327 at 362.068 kb on + strand, within GFF327 at 362.068 kb on + strand, within GFF327 at 362.068 kb on + strand, within GFF327 at 362.068 kb on + strand, within GFF327 at 362.068 kb on + strand, within GFF327 at 362.068 kb on + strand, within GFF327 at 362.068 kb on + strand, within GFF327 at 362.069 kb on - strand, within GFF327 at 362.069 kb on - strand, within GFF327 at 362.069 kb on - strand, within GFF327 at 362.227 kb on + strand at 362.228 kb on - strand at 362.320 kb on - strand, within GFF328 at 362.343 kb on + strand, within GFF328 at 362.344 kb on - strand, within GFF328 at 362.732 kb on - strand, within GFF328 at 362.748 kb on + strand, within GFF328 at 362.748 kb on + strand, within GFF328 at 362.748 kb on + strand, within GFF328 at 362.748 kb on + strand, within GFF328 at 362.748 kb on + strand, within GFF328 at 362.748 kb on + strand, within GFF328 at 362.748 kb on + strand, within GFF328 at 362.748 kb on + strand, within GFF328 at 362.748 kb on + strand, within GFF328 at 362.748 kb on + strand, within GFF328 at 362.748 kb on + strand, within GFF328 at 362.748 kb on + strand, within GFF328 at 362.749 kb on - strand, within GFF328 at 362.749 kb on - strand, within GFF328 at 362.749 kb on - strand, within GFF328 at 362.749 kb on - strand, within GFF328 at 362.749 kb on - strand, within GFF328 at 362.749 kb on - strand, within GFF328 at 362.749 kb on - strand, within GFF328 at 362.749 kb on - strand, within GFF328 at 362.749 kb on - strand, within GFF328 at 362.795 kb on + strand, within GFF328 at 362.889 kb on - strand at 363.098 kb on + strand, within GFF329 at 363.098 kb on + strand, within GFF329 at 363.098 kb on + strand, within GFF329 at 363.098 kb on + strand, within GFF329 at 363.098 kb on + strand, within GFF329 at 363.098 kb on + strand, within GFF329 at 363.099 kb on - strand, within GFF329 at 363.099 kb on - strand, within GFF329 at 363.099 kb on - strand, within GFF329 at 363.099 kb on - strand, within GFF329 at 363.405 kb on - strand, within GFF329
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 361,324 + GFF327 0.10 +0.9 361,324 + GFF327 0.10 -0.7 361,324 + GFF327 0.10 +1.2 361,324 + GFF327 0.10 -0.4 361,325 - GFF327 0.11 -0.1 361,666 + GFF327 0.45 +0.8 361,667 - GFF327 0.46 +0.0 361,667 - GFF327 0.46 -1.3 361,708 + GFF327 0.50 +1.3 361,708 + GFF327 0.50 -0.6 361,708 + GFF327 0.50 +0.9 361,709 - GFF327 0.50 +0.6 361,709 - GFF327 0.50 -1.0 361,709 - GFF327 0.50 +0.0 361,975 + GFF327 0.77 -1.3 361,984 + GFF327 0.78 +0.0 361,984 + GFF327 0.78 -1.2 361,984 + GFF327 0.78 -0.3 361,984 + GFF327 0.78 -0.6 361,984 + GFF327 0.78 -0.2 361,984 + GFF327 0.78 +0.3 361,985 - GFF327 0.78 -0.8 361,985 - GFF327 0.78 -0.4 361,985 - GFF327 0.78 +0.7 361,990 + GFF327 0.79 +0.3 361,990 + GFF327 0.79 +3.1 361,990 + GFF327 0.79 -1.5 361,990 + GFF327 0.79 -0.7 361,990 + GFF327 0.79 -1.3 361,990 + GFF327 0.79 -0.1 361,990 + GFF327 0.79 -0.8 361,990 + GFF327 0.79 -0.3 361,990 + GFF327 0.79 -0.5 361,990 + GFF327 0.79 +0.3 361,990 + GFF327 0.79 -0.7 361,990 + GFF327 0.79 -1.3 361,990 + GFF327 0.79 +0.4 361,990 + GFF327 0.79 +0.2 361,990 + GFF327 0.79 -2.3 361,991 - GFF327 0.79 -0.5 361,991 - GFF327 0.79 +0.9 361,991 - GFF327 0.79 +0.3 361,991 - GFF327 0.79 +0.7 361,991 - GFF327 0.79 -1.6 361,991 - GFF327 0.79 -2.2 361,991 - GFF327 0.79 -0.0 361,991 - GFF327 0.79 +1.0 361,991 - GFF327 0.79 +1.3 361,991 - GFF327 0.79 -0.0 361,991 - GFF327 0.79 +0.5 362,050 + GFF327 0.85 -0.2 362,051 - GFF327 0.85 -0.1 362,051 - GFF327 0.85 +0.1 362,068 + GFF327 0.87 -0.7 362,068 + GFF327 0.87 -0.3 362,068 + GFF327 0.87 -0.5 362,068 + GFF327 0.87 -0.8 362,068 + GFF327 0.87 -0.5 362,068 + GFF327 0.87 +0.8 362,068 + GFF327 0.87 -1.2 362,069 - GFF327 0.87 -0.5 362,069 - GFF327 0.87 -0.2 362,069 - GFF327 0.87 -0.3 362,227 + -0.6 362,228 - -1.5 362,320 - GFF328 0.11 -0.3 362,343 + GFF328 0.14 -0.3 362,344 - GFF328 0.14 -0.7 362,732 - GFF328 0.70 -0.1 362,748 + GFF328 0.72 -0.2 362,748 + GFF328 0.72 -2.3 362,748 + GFF328 0.72 -0.5 362,748 + GFF328 0.72 -1.2 362,748 + GFF328 0.72 -1.3 362,748 + GFF328 0.72 +0.4 362,748 + GFF328 0.72 -0.1 362,748 + GFF328 0.72 -2.6 362,748 + GFF328 0.72 -0.9 362,748 + GFF328 0.72 -0.2 362,748 + GFF328 0.72 +0.3 362,748 + GFF328 0.72 -0.1 362,749 - GFF328 0.73 -1.1 362,749 - GFF328 0.73 -0.3 362,749 - GFF328 0.73 +0.1 362,749 - GFF328 0.73 +1.0 362,749 - GFF328 0.73 -0.1 362,749 - GFF328 0.73 -0.2 362,749 - GFF328 0.73 +1.3 362,749 - GFF328 0.73 -0.9 362,749 - GFF328 0.73 -0.5 362,795 + GFF328 0.79 -1.4 362,889 - -2.0 363,098 + GFF329 0.10 -0.5 363,098 + GFF329 0.10 -0.7 363,098 + GFF329 0.10 +0.1 363,098 + GFF329 0.10 -0.6 363,098 + GFF329 0.10 +1.0 363,098 + GFF329 0.10 -0.0 363,099 - GFF329 0.10 +0.4 363,099 - GFF329 0.10 -0.6 363,099 - GFF329 0.10 +0.0 363,099 - GFF329 0.10 -0.2 363,405 - GFF329 0.29 -0.3
Or see this region's nucleotide sequence