Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3219 and GFF3220 are separated by 24 nucleotides GFF3220 and GFF3221 are separated by 3 nucleotides GFF3221 and GFF3222 are separated by 128 nucleotides GFF3222 and GFF3223 are separated by 1 nucleotides
GFF3219 - hypothetical protein, at 38,717 to 39,154
GFF3219
GFF3220 - no description, at 39,179 to 39,790
GFF3220
GFF3221 - hypothetical protein, at 39,794 to 43,843
GFF3221
GFF3222 - no description, at 43,972 to 44,388
GFF3222
GFF3223 - no description, at 44,390 to 45,007
GFF3223
Position (kb)
39
40
41
42
43
44 Strain fitness (log2 ratio)
-2
-1
0
1 at 38.974 kb on - strand, within GFF3219 at 38.974 kb on - strand, within GFF3219 at 38.984 kb on + strand, within GFF3219 at 38.985 kb on - strand, within GFF3219 at 38.985 kb on - strand, within GFF3219 at 38.985 kb on - strand, within GFF3219 at 38.985 kb on - strand, within GFF3219 at 38.985 kb on - strand, within GFF3219 at 39.045 kb on + strand, within GFF3219 at 39.045 kb on + strand, within GFF3219 at 39.045 kb on + strand, within GFF3219 at 39.045 kb on + strand, within GFF3219 at 39.045 kb on + strand, within GFF3219 at 39.046 kb on - strand, within GFF3219 at 39.147 kb on + strand at 39.180 kb on + strand at 39.181 kb on - strand at 39.636 kb on + strand, within GFF3220 at 39.771 kb on + strand at 39.816 kb on + strand at 40.117 kb on + strand at 40.117 kb on + strand at 40.377 kb on + strand, within GFF3221 at 40.457 kb on - strand, within GFF3221 at 40.457 kb on - strand, within GFF3221 at 40.541 kb on + strand, within GFF3221 at 40.828 kb on + strand, within GFF3221 at 40.829 kb on - strand, within GFF3221 at 40.829 kb on - strand, within GFF3221 at 41.535 kb on + strand, within GFF3221 at 41.583 kb on + strand, within GFF3221 at 41.583 kb on + strand, within GFF3221 at 41.583 kb on + strand, within GFF3221 at 41.584 kb on - strand, within GFF3221 at 41.685 kb on + strand, within GFF3221 at 41.685 kb on + strand, within GFF3221 at 41.686 kb on - strand, within GFF3221 at 41.828 kb on - strand, within GFF3221 at 42.093 kb on + strand, within GFF3221 at 42.105 kb on + strand, within GFF3221 at 42.105 kb on + strand, within GFF3221 at 42.106 kb on - strand, within GFF3221 at 42.135 kb on + strand, within GFF3221 at 42.444 kb on + strand, within GFF3221 at 42.445 kb on - strand, within GFF3221 at 42.445 kb on - strand, within GFF3221 at 42.445 kb on - strand, within GFF3221 at 42.498 kb on + strand, within GFF3221 at 42.498 kb on + strand, within GFF3221 at 42.498 kb on + strand, within GFF3221 at 42.498 kb on + strand, within GFF3221 at 42.498 kb on + strand, within GFF3221 at 42.498 kb on + strand, within GFF3221 at 42.540 kb on + strand, within GFF3221 at 42.793 kb on - strand, within GFF3221 at 42.793 kb on - strand, within GFF3221 at 42.793 kb on - strand, within GFF3221 at 43.023 kb on + strand, within GFF3221 at 43.024 kb on - strand, within GFF3221 at 43.024 kb on - strand, within GFF3221 at 43.024 kb on - strand, within GFF3221 at 43.024 kb on - strand, within GFF3221 at 43.044 kb on + strand, within GFF3221 at 43.044 kb on + strand, within GFF3221 at 43.044 kb on + strand, within GFF3221 at 43.045 kb on - strand, within GFF3221 at 43.080 kb on + strand, within GFF3221 at 43.081 kb on - strand, within GFF3221 at 43.081 kb on - strand, within GFF3221 at 43.153 kb on - strand, within GFF3221 at 43.206 kb on + strand, within GFF3221 at 43.320 kb on + strand, within GFF3221 at 43.320 kb on + strand, within GFF3221 at 43.320 kb on + strand, within GFF3221 at 43.320 kb on + strand, within GFF3221 at 43.320 kb on + strand, within GFF3221 at 43.320 kb on + strand, within GFF3221 at 43.321 kb on - strand, within GFF3221 at 43.321 kb on - strand, within GFF3221 at 43.321 kb on - strand, within GFF3221 at 43.321 kb on - strand, within GFF3221 at 43.321 kb on - strand, within GFF3221 at 43.321 kb on - strand, within GFF3221 at 43.321 kb on - strand, within GFF3221 at 43.327 kb on - strand, within GFF3221 at 43.398 kb on + strand, within GFF3221 at 43.398 kb on + strand, within GFF3221 at 43.398 kb on + strand, within GFF3221 at 43.399 kb on - strand, within GFF3221 at 43.399 kb on - strand, within GFF3221 at 43.399 kb on - strand, within GFF3221 at 43.399 kb on - strand, within GFF3221 at 43.512 kb on + strand at 43.513 kb on - strand at 43.621 kb on - strand at 43.677 kb on + strand at 43.677 kb on + strand at 43.677 kb on + strand at 43.678 kb on - strand at 43.678 kb on - strand at 43.678 kb on - strand at 43.678 kb on - strand at 43.831 kb on + strand at 44.001 kb on + strand at 44.072 kb on + strand, within GFF3222
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 38,974 - GFF3219 0.59 -1.0 38,974 - GFF3219 0.59 -0.7 38,984 + GFF3219 0.61 +0.3 38,985 - GFF3219 0.61 -0.4 38,985 - GFF3219 0.61 -1.0 38,985 - GFF3219 0.61 -0.3 38,985 - GFF3219 0.61 +0.9 38,985 - GFF3219 0.61 -1.0 39,045 + GFF3219 0.75 -0.3 39,045 + GFF3219 0.75 +0.3 39,045 + GFF3219 0.75 -0.4 39,045 + GFF3219 0.75 +1.5 39,045 + GFF3219 0.75 -0.7 39,046 - GFF3219 0.75 +0.7 39,147 + +0.8 39,180 + -0.3 39,181 - -0.6 39,636 + GFF3220 0.75 -1.3 39,771 + -0.1 39,816 + -1.5 40,117 + +1.4 40,117 + +0.1 40,377 + GFF3221 0.14 -1.3 40,457 - GFF3221 0.16 -1.0 40,457 - GFF3221 0.16 -0.6 40,541 + GFF3221 0.18 -0.8 40,828 + GFF3221 0.26 -1.3 40,829 - GFF3221 0.26 -0.8 40,829 - GFF3221 0.26 +0.3 41,535 + GFF3221 0.43 +0.7 41,583 + GFF3221 0.44 -0.5 41,583 + GFF3221 0.44 +0.7 41,583 + GFF3221 0.44 +0.4 41,584 - GFF3221 0.44 -0.9 41,685 + GFF3221 0.47 -0.5 41,685 + GFF3221 0.47 +1.2 41,686 - GFF3221 0.47 +1.0 41,828 - GFF3221 0.50 -0.5 42,093 + GFF3221 0.57 +0.1 42,105 + GFF3221 0.57 -0.5 42,105 + GFF3221 0.57 +0.1 42,106 - GFF3221 0.57 -1.8 42,135 + GFF3221 0.58 -0.3 42,444 + GFF3221 0.65 -1.2 42,445 - GFF3221 0.65 -1.6 42,445 - GFF3221 0.65 -0.5 42,445 - GFF3221 0.65 -0.2 42,498 + GFF3221 0.67 -0.4 42,498 + GFF3221 0.67 +0.9 42,498 + GFF3221 0.67 +0.1 42,498 + GFF3221 0.67 -0.2 42,498 + GFF3221 0.67 +1.0 42,498 + GFF3221 0.67 +0.1 42,540 + GFF3221 0.68 -0.1 42,793 - GFF3221 0.74 +0.1 42,793 - GFF3221 0.74 -0.4 42,793 - GFF3221 0.74 +0.4 43,023 + GFF3221 0.80 -0.3 43,024 - GFF3221 0.80 +0.6 43,024 - GFF3221 0.80 -0.4 43,024 - GFF3221 0.80 -1.3 43,024 - GFF3221 0.80 -1.8 43,044 + GFF3221 0.80 -1.3 43,044 + GFF3221 0.80 -1.0 43,044 + GFF3221 0.80 -0.6 43,045 - GFF3221 0.80 -0.1 43,080 + GFF3221 0.81 -0.5 43,081 - GFF3221 0.81 -0.2 43,081 - GFF3221 0.81 +0.9 43,153 - GFF3221 0.83 -2.1 43,206 + GFF3221 0.84 -1.3 43,320 + GFF3221 0.87 -0.5 43,320 + GFF3221 0.87 +1.3 43,320 + GFF3221 0.87 -1.0 43,320 + GFF3221 0.87 +0.7 43,320 + GFF3221 0.87 +0.3 43,320 + GFF3221 0.87 -1.5 43,321 - GFF3221 0.87 -1.2 43,321 - GFF3221 0.87 -0.7 43,321 - GFF3221 0.87 -0.3 43,321 - GFF3221 0.87 +1.4 43,321 - GFF3221 0.87 -0.6 43,321 - GFF3221 0.87 -1.0 43,321 - GFF3221 0.87 -0.8 43,327 - GFF3221 0.87 +0.3 43,398 + GFF3221 0.89 -0.5 43,398 + GFF3221 0.89 -1.6 43,398 + GFF3221 0.89 +0.5 43,399 - GFF3221 0.89 +0.1 43,399 - GFF3221 0.89 +1.4 43,399 - GFF3221 0.89 +0.1 43,399 - GFF3221 0.89 -0.4 43,512 + +0.4 43,513 - -1.1 43,621 - +0.1 43,677 + -0.5 43,677 + +0.4 43,677 + -0.5 43,678 - -0.7 43,678 - +0.4 43,678 - -0.5 43,678 - +0.4 43,831 + +0.3 44,001 + -1.8 44,072 + GFF3222 0.24 -0.3
Or see this region's nucleotide sequence