Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3023 and GFF3024 are separated by 13 nucleotides GFF3024 and GFF3025 overlap by 13 nucleotides GFF3025 and GFF3026 overlap by 4 nucleotides GFF3026 and GFF3027 are separated by 20 nucleotides
GFF3023 - C4-dicarboxylate transport protein, at 451,223 to 452,497
GFF3023
GFF3024 - Aspartate racemase (EC 5.1.1.13), at 452,511 to 453,245
GFF3024
GFF3025 - Proton/sodium-glutamate symport protein, at 453,233 to 454,702
GFF3025
GFF3026 - KEGG: type VI secretion system protein, at 454,699 to 455,172
GFF3026
GFF3027 - T6SS component TssK (ImpJ/VasE), at 455,193 to 456,515
GFF3027
Position (kb) 453
454
455 Strain fitness (log2 ratio) -1
0
1
2 at 452.234 kb on + strand, within GFF3023 at 452.234 kb on + strand, within GFF3023 at 452.358 kb on - strand, within GFF3023 at 452.490 kb on - strand at 452.495 kb on + strand at 452.495 kb on + strand at 452.917 kb on - strand, within GFF3024 at 453.311 kb on + strand at 453.312 kb on - strand at 453.312 kb on - strand at 453.356 kb on + strand at 453.356 kb on + strand at 453.356 kb on + strand at 453.356 kb on + strand at 453.356 kb on + strand at 453.356 kb on + strand at 453.357 kb on - strand at 453.357 kb on - strand at 453.357 kb on - strand at 453.627 kb on - strand, within GFF3025 at 453.815 kb on + strand, within GFF3025 at 453.815 kb on + strand, within GFF3025 at 453.816 kb on - strand, within GFF3025 at 453.816 kb on - strand, within GFF3025 at 454.196 kb on + strand, within GFF3025 at 454.196 kb on + strand, within GFF3025 at 454.197 kb on - strand, within GFF3025 at 454.197 kb on - strand, within GFF3025 at 454.199 kb on + strand, within GFF3025 at 454.200 kb on - strand, within GFF3025 at 454.232 kb on + strand, within GFF3025 at 454.233 kb on - strand, within GFF3025 at 454.662 kb on - strand at 454.976 kb on - strand, within GFF3026 at 455.071 kb on + strand, within GFF3026 at 455.071 kb on + strand, within GFF3026 at 455.071 kb on + strand, within GFF3026 at 455.072 kb on - strand, within GFF3026 at 455.072 kb on - strand, within GFF3026 at 455.518 kb on - strand, within GFF3027 at 455.518 kb on - strand, within GFF3027
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 452,234 + GFF3023 0.79 +0.3 452,234 + GFF3023 0.79 -0.9 452,358 - GFF3023 0.89 -0.2 452,490 - -0.3 452,495 + +0.1 452,495 + -0.9 452,917 - GFF3024 0.55 -0.2 453,311 + -0.3 453,312 - -0.2 453,312 - +0.4 453,356 + +0.6 453,356 + -1.2 453,356 + -0.5 453,356 + +1.3 453,356 + -0.1 453,356 + -0.1 453,357 - -0.5 453,357 - -0.6 453,357 - +0.3 453,627 - GFF3025 0.27 -0.1 453,815 + GFF3025 0.40 +0.9 453,815 + GFF3025 0.40 -0.3 453,816 - GFF3025 0.40 -0.8 453,816 - GFF3025 0.40 -1.2 454,196 + GFF3025 0.66 +0.6 454,196 + GFF3025 0.66 -0.1 454,197 - GFF3025 0.66 +0.5 454,197 - GFF3025 0.66 -0.4 454,199 + GFF3025 0.66 +0.5 454,200 - GFF3025 0.66 +0.3 454,232 + GFF3025 0.68 +2.1 454,233 - GFF3025 0.68 -0.7 454,662 - +1.0 454,976 - GFF3026 0.58 +0.4 455,071 + GFF3026 0.78 -1.0 455,071 + GFF3026 0.78 -0.5 455,071 + GFF3026 0.78 -0.4 455,072 - GFF3026 0.79 +0.3 455,072 - GFF3026 0.79 +0.0 455,518 - GFF3027 0.25 -0.6 455,518 - GFF3027 0.25 +1.0
Or see this region's nucleotide sequence