Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF295 and GFF296 are separated by 10 nucleotides GFF296 and GFF297 are separated by 3 nucleotides GFF297 and GFF298 overlap by 4 nucleotides GFF298 and GFF299 overlap by 4 nucleotides
GFF295 - Ribonuclease G, at 329,644 to 331,170
GFF295
GFF296 - Septum formation protein Maf, at 331,181 to 331,783
GFF296
GFF297 - FIG00978250: hypothetical protein, at 331,787 to 332,500
GFF297
GFF298 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase (EC 2.1.1.177), at 332,497 to 332,964
GFF298
GFF299 - Ribosomal silencing factor RsfA, at 332,961 to 333,647
GFF299
Position (kb)
331
332
333 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 331.049 kb on + strand at 331.050 kb on - strand at 331.050 kb on - strand at 331.186 kb on + strand at 331.187 kb on - strand at 331.187 kb on - strand at 331.187 kb on - strand at 331.187 kb on - strand at 331.238 kb on + strand at 331.251 kb on + strand, within GFF296 at 331.252 kb on - strand, within GFF296 at 331.302 kb on + strand, within GFF296 at 331.302 kb on + strand, within GFF296 at 331.302 kb on + strand, within GFF296 at 331.302 kb on + strand, within GFF296 at 331.302 kb on + strand, within GFF296 at 331.303 kb on - strand, within GFF296 at 331.303 kb on - strand, within GFF296 at 331.303 kb on - strand, within GFF296 at 331.303 kb on - strand, within GFF296 at 331.303 kb on - strand, within GFF296 at 331.303 kb on - strand, within GFF296 at 331.303 kb on - strand, within GFF296 at 331.303 kb on - strand, within GFF296 at 331.342 kb on - strand, within GFF296 at 331.632 kb on + strand, within GFF296 at 331.632 kb on + strand, within GFF296 at 331.632 kb on + strand, within GFF296 at 331.632 kb on + strand, within GFF296 at 331.632 kb on + strand, within GFF296 at 331.632 kb on + strand, within GFF296 at 331.633 kb on - strand, within GFF296 at 331.633 kb on - strand, within GFF296 at 331.633 kb on - strand, within GFF296 at 331.633 kb on - strand, within GFF296 at 331.633 kb on - strand, within GFF296 at 331.633 kb on - strand, within GFF296 at 331.633 kb on - strand, within GFF296 at 331.633 kb on - strand, within GFF296 at 331.703 kb on + strand, within GFF296 at 331.703 kb on + strand, within GFF296 at 331.703 kb on + strand, within GFF296 at 331.704 kb on - strand, within GFF296 at 331.704 kb on - strand, within GFF296 at 331.704 kb on - strand, within GFF296 at 331.704 kb on - strand, within GFF296 at 331.767 kb on + strand at 331.767 kb on + strand at 331.767 kb on + strand at 331.768 kb on - strand at 331.768 kb on - strand at 332.095 kb on + strand, within GFF297 at 332.095 kb on + strand, within GFF297 at 332.095 kb on + strand, within GFF297 at 332.095 kb on + strand, within GFF297 at 332.095 kb on + strand, within GFF297 at 332.096 kb on - strand, within GFF297 at 332.096 kb on - strand, within GFF297 at 332.096 kb on - strand, within GFF297 at 332.105 kb on + strand, within GFF297 at 332.106 kb on - strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.149 kb on - strand, within GFF297 at 332.149 kb on - strand, within GFF297 at 332.149 kb on - strand, within GFF297 at 332.149 kb on - strand, within GFF297 at 332.150 kb on - strand, within GFF297 at 332.150 kb on - strand, within GFF297 at 332.454 kb on + strand at 332.454 kb on + strand at 332.454 kb on + strand at 332.510 kb on + strand at 332.510 kb on + strand at 332.510 kb on + strand at 332.510 kb on + strand at 332.510 kb on + strand at 332.511 kb on - strand at 332.511 kb on - strand at 332.511 kb on - strand at 332.511 kb on - strand at 332.543 kb on + strand at 332.810 kb on + strand, within GFF298 at 332.810 kb on + strand, within GFF298 at 332.810 kb on + strand, within GFF298 at 332.810 kb on + strand, within GFF298 at 332.810 kb on + strand, within GFF298 at 332.810 kb on + strand, within GFF298 at 332.810 kb on + strand, within GFF298 at 332.811 kb on - strand, within GFF298 at 332.811 kb on - strand, within GFF298 at 332.811 kb on - strand, within GFF298 at 332.811 kb on - strand, within GFF298 at 332.897 kb on + strand, within GFF298 at 332.897 kb on + strand, within GFF298 at 332.898 kb on - strand, within GFF298 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.334 kb on + strand, within GFF299 at 333.334 kb on + strand, within GFF299 at 333.334 kb on + strand, within GFF299 at 333.334 kb on + strand, within GFF299 at 333.334 kb on + strand, within GFF299 at 333.334 kb on + strand, within GFF299 at 333.334 kb on + strand, within GFF299 at 333.334 kb on + strand, within GFF299 at 333.335 kb on - strand, within GFF299 at 333.335 kb on - strand, within GFF299 at 333.335 kb on - strand, within GFF299 at 333.335 kb on - strand, within GFF299 at 333.335 kb on - strand, within GFF299
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 331,049 + -1.0 331,050 - -1.0 331,050 - -1.9 331,186 + -0.5 331,187 - -0.7 331,187 - -0.2 331,187 - +0.2 331,187 - +0.3 331,238 + +0.1 331,251 + GFF296 0.12 +0.9 331,252 - GFF296 0.12 -0.6 331,302 + GFF296 0.20 +1.0 331,302 + GFF296 0.20 +0.3 331,302 + GFF296 0.20 -0.0 331,302 + GFF296 0.20 +0.1 331,302 + GFF296 0.20 -1.3 331,303 - GFF296 0.20 -0.5 331,303 - GFF296 0.20 -0.7 331,303 - GFF296 0.20 -0.5 331,303 - GFF296 0.20 -0.2 331,303 - GFF296 0.20 -0.8 331,303 - GFF296 0.20 +0.0 331,303 - GFF296 0.20 -0.4 331,303 - GFF296 0.20 -1.9 331,342 - GFF296 0.27 -1.0 331,632 + GFF296 0.75 +0.9 331,632 + GFF296 0.75 +0.1 331,632 + GFF296 0.75 -0.8 331,632 + GFF296 0.75 +1.3 331,632 + GFF296 0.75 -0.2 331,632 + GFF296 0.75 -0.0 331,633 - GFF296 0.75 -0.5 331,633 - GFF296 0.75 -0.6 331,633 - GFF296 0.75 -1.0 331,633 - GFF296 0.75 -0.4 331,633 - GFF296 0.75 +0.4 331,633 - GFF296 0.75 -0.3 331,633 - GFF296 0.75 -0.9 331,633 - GFF296 0.75 -1.6 331,703 + GFF296 0.87 +0.2 331,703 + GFF296 0.87 -0.6 331,703 + GFF296 0.87 -0.6 331,704 - GFF296 0.87 -2.8 331,704 - GFF296 0.87 -0.3 331,704 - GFF296 0.87 +0.5 331,704 - GFF296 0.87 +0.3 331,767 + +1.0 331,767 + +0.1 331,767 + -0.8 331,768 - +0.0 331,768 - -1.2 332,095 + GFF297 0.43 -0.1 332,095 + GFF297 0.43 +1.3 332,095 + GFF297 0.43 -0.3 332,095 + GFF297 0.43 -0.3 332,095 + GFF297 0.43 +0.2 332,096 - GFF297 0.43 +0.5 332,096 - GFF297 0.43 +2.3 332,096 - GFF297 0.43 -1.0 332,105 + GFF297 0.45 -0.0 332,106 - GFF297 0.45 +0.5 332,148 + GFF297 0.51 -0.2 332,148 + GFF297 0.51 -0.7 332,148 + GFF297 0.51 -0.5 332,148 + GFF297 0.51 -0.4 332,148 + GFF297 0.51 +0.5 332,148 + GFF297 0.51 -0.8 332,148 + GFF297 0.51 -0.0 332,148 + GFF297 0.51 +0.9 332,148 + GFF297 0.51 -0.1 332,148 + GFF297 0.51 -0.7 332,148 + GFF297 0.51 -0.0 332,149 - GFF297 0.51 +0.3 332,149 - GFF297 0.51 +0.0 332,149 - GFF297 0.51 -0.7 332,149 - GFF297 0.51 +0.4 332,150 - GFF297 0.51 +0.3 332,150 - GFF297 0.51 -0.3 332,454 + -0.0 332,454 + -0.6 332,454 + +0.5 332,510 + -0.3 332,510 + -1.0 332,510 + -0.2 332,510 + -1.1 332,510 + +0.8 332,511 - -0.1 332,511 - -1.1 332,511 - +0.2 332,511 - +0.1 332,543 + +0.1 332,810 + GFF298 0.67 +0.2 332,810 + GFF298 0.67 -0.4 332,810 + GFF298 0.67 -0.2 332,810 + GFF298 0.67 -0.1 332,810 + GFF298 0.67 +0.2 332,810 + GFF298 0.67 -0.6 332,810 + GFF298 0.67 -0.1 332,811 - GFF298 0.67 -0.5 332,811 - GFF298 0.67 -1.0 332,811 - GFF298 0.67 +0.1 332,811 - GFF298 0.67 +0.4 332,897 + GFF298 0.85 -0.7 332,897 + GFF298 0.85 +1.3 332,898 - GFF298 0.86 +0.1 333,331 + GFF299 0.54 +0.4 333,331 + GFF299 0.54 -0.5 333,331 + GFF299 0.54 -1.0 333,331 + GFF299 0.54 -2.2 333,331 + GFF299 0.54 +0.3 333,331 + GFF299 0.54 -1.0 333,331 + GFF299 0.54 -1.0 333,331 + GFF299 0.54 -0.0 333,331 + GFF299 0.54 -0.9 333,331 + GFF299 0.54 -2.0 333,331 + GFF299 0.54 -0.7 333,331 + GFF299 0.54 -0.3 333,331 + GFF299 0.54 -0.5 333,331 + GFF299 0.54 +0.0 333,332 - GFF299 0.54 -1.0 333,332 - GFF299 0.54 -0.5 333,332 - GFF299 0.54 +0.5 333,332 - GFF299 0.54 -0.5 333,332 - GFF299 0.54 -0.3 333,332 - GFF299 0.54 -1.2 333,332 - GFF299 0.54 -1.6 333,332 - GFF299 0.54 -1.0 333,332 - GFF299 0.54 -1.0 333,332 - GFF299 0.54 +0.5 333,332 - GFF299 0.54 +0.2 333,332 - GFF299 0.54 -1.0 333,334 + GFF299 0.54 +0.3 333,334 + GFF299 0.54 -0.1 333,334 + GFF299 0.54 -0.3 333,334 + GFF299 0.54 +0.1 333,334 + GFF299 0.54 +0.7 333,334 + GFF299 0.54 -0.0 333,334 + GFF299 0.54 -2.7 333,334 + GFF299 0.54 -0.8 333,335 - GFF299 0.54 +0.9 333,335 - GFF299 0.54 +2.3 333,335 - GFF299 0.54 -0.6 333,335 - GFF299 0.54 +0.4 333,335 - GFF299 0.54 +0.2
Or see this region's nucleotide sequence